leuO

Summary

Gene Symbol: leuO
Description: global transcription factor
Alias: ECK0078, JW0075
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Stratmann T, Pul U, Wurm R, Wagner R, Schnetz K. RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants. Mol Microbiol. 2012;83:1109-23 pubmed publisher
    The LysR-type transcription factor LeuO is involved in regulation of pathogenicity determinants and stress responses in Enterobacteriaceae, and acts as antagonist of the global repressor H-NS...
  2. Shimada T, Bridier A, Briandet R, Ishihama A. Novel roles of LeuO in transcription regulation of E.?coli genome: antagonistic interplay with the universal silencer H-NS. Mol Microbiol. 2011;82:378-97 pubmed publisher
    b>LeuO, the regulator of leucine biosynthesis operon of Escherichia coli, is involved in the regulation of as yet unspecified genes affecting the stress response and pathogenesis expression...
  3. Westra E, Pul U, Heidrich N, Jore M, Lundgren M, Stratmann T, et al. H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO. Mol Microbiol. 2010;77:1380-93 pubmed publisher
    ..In addition, it is demonstrated that LeuO, a LysR-type transcription factor, binds to two sites flanking the casA promoter and the H-NS nucleation site, ..
  4. Shimada T, Yamamoto K, Ishihama A. Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux. J Bacteriol. 2009;191:4562-71 pubmed publisher
    b>LeuO, a LysR family transcription factor, exists in a wide variety of bacteria of the family Enterobacteriaceae and is involved in the regulation of as yet unidentified genes affecting the stress response and pathogenesis expression...
  5. Chen C, Chou M, Huang C, Majumder A, Wu H. A cis-spreading nucleoprotein filament is responsible for the gene silencing activity found in the promoter relay mechanism. J Biol Chem. 2005;280:5101-12 pubmed
    ..role in a promoter relay mechanism for the coordinated expression of genes in the Salmonella typhimurium ilvIH-leuO-leuABCD gene cluster...
  6. Ohta T, Ueguchi C, Mizuno T. rpoS function is essential for bgl silencing caused by C-terminally truncated H-NS in Escherichia coli. J Bacteriol. 1999;181:6278-83 pubmed
    ..These isolated mutations were mapped to five loci on the chromosome. Among these loci, three appeared to be leuO, hns, and bglJ, which were previously characterized, while the other two were novel...
  7. Chen C, Ghole M, Majumder A, Wang Z, Chandana S, Wu H. LeuO-mediated transcriptional derepression. J Biol Chem. 2003;278:38094-103 pubmed
    To understand the coordination of gene expression in the Salmonella typhimurium ilvIH-leuO-leuABCD gene cluster, we had previously identified a 72-bp AT-rich (78% A+T) DNA sequence element, AT4, which was capable of silencing ..
  8. Repoila F, Gottesman S. Signal transduction cascade for regulation of RpoS: temperature regulation of DsrA. J Bacteriol. 2001;183:4012-23 pubmed
    ..Furthermore, two activating regions and a site for LeuO negative regulation were identified in the dsrA promoter...
  9. Breddermann H, Schnetz K. Correlation of Antagonistic Regulation of leuO Transcription with the Cellular Levels of BglJ-RcsB and LeuO in Escherichia coli. Front Cell Infect Microbiol. 2016;6:106 pubmed
    b>LeuO is a conserved and pleiotropic transcription regulator, antagonist of the nucleoid-associated silencer protein H-NS, and important for pathogenicity and multidrug resistance in Enterobacteriaceae...

More Information

Publications24

  1. Stratmann T, Madhusudan S, Schnetz K. Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO. J Bacteriol. 2008;190:926-35 pubmed
    ..The LysR-type transcription factor LeuO counteracts this repression...
  2. Repoila F, Gottesman S. Temperature sensing by the dsrA promoter. J Bacteriol. 2003;185:6609-14 pubmed
    ..These results show a complex interdependence of different regions in the promoter for temperature regulation. ..
  3. Fang M, Wu H. Suppression of leu-500 mutation in topA+ Salmonella typhimurium strains. The promoter relay at work. J Biol Chem. 1998;273:29929-34 pubmed
    ..addition, suppression of the chromosomal leu-500 mutation correlates with the transcription activities of ilvIH and leuO rather than the TopA level in the topA+ strain...
  4. Fang M, Majumder A, Tsai K, Wu H. ppGpp-dependent leuO expression in bacteria under stress. Biochem Biophys Res Commun. 2000;276:64-70 pubmed publisher
    Despite the known potential transcription regulatory role of leuO gene product, LeuO, the condition when leuO expresses during bacterial growth cycle remains unclear...
  5. Chen C, Wu H. LeuO protein delimits the transcriptionally active and repressive domains on the bacterial chromosome. J Biol Chem. 2005;280:15111-21 pubmed
    b>LeuO protein relieves bacterial gene silencer AT8-mediated transcriptional repression as part of a promoter relay mechanism found in the ilvIH-leuO-leuABCD gene cluster...
  6. Fang M, Wu H. A promoter relay mechanism for sequential gene activation. J Bacteriol. 1998;180:626-33 pubmed
    ..genes are sequentially expressed in a position-dependent manner: the ilvIH promoter (pilvIH) activates a cryptic leuO promoter (pleuO) located between the two divergently arrayed ilvIH and leu-500 promoters...
  7. Shi X, Bennett G. Effects of multicopy LeuO on the expression of the acid-inducible lysine decarboxylase gene in Escherichia coli. J Bacteriol. 1995;177:810-4 pubmed
    ..A previously sequenced open reading frame, leuO, proves to be the gene that causes the hns-complementing phenotype...
  8. Majumder A, Fang M, Tsai K, Ueguchi C, Mizuno T, Wu H. LeuO expression in response to starvation for branched-chain amino acids. J Biol Chem. 2001;276:19046-51 pubmed
    The recently identified role of LeuO in the regulation of transcription has prompted us to search for the specific function(s) of LeuO in bacterial physiology. The cryptic nature of expression of leuO has previously limited such analysis...
  9. Chen C, Fang M, Majumder A, Wu H. A 72-base pair AT-rich DNA sequence element functions as a bacterial gene silencer. J Biol Chem. 2001;276:9478-85 pubmed
    We have previously demonstrated that sequential activation of the bacterial ilvIH-leuO-leuABCD gene cluster involves a promoter-relay mechanism...
  10. Ueguchi C, Ohta T, Seto C, Suzuki T, Mizuno T. The leuO gene product has a latent ability to relieve bgl silencing in Escherichia coli. J Bacteriol. 1998;180:190-3 pubmed
    ..In this case, the insertion mutation was found to be just in front of the leuO gene encoding a putative LysR-like DNA-binding protein...
  11. Hertzberg K, Gemmill R, Jones J, Calvo J. Cloning of an EcoRI-generated fragment of the leucine operon of Salmonella typhimurium. Gene. 1980;8:135-52 pubmed
    ..and derepression and from prototrophic strains derepressed for the leucine operon as a result of mutations in leuO, leuS, and flrB...
  12. Henikoff S, Haughn G, Calvo J, Wallace J. A large family of bacterial activator proteins. Proc Natl Acad Sci U S A. 1988;85:6602-6 pubmed
    ..The DNA database searching procedure and other methods used in this study should be useful in detecting other groups of related proteins. ..
  13. Haughn G, Wessler S, Gemmill R, Calvo J. High A + T content conserved in DNA sequences upstream of leuABCD in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1986;166:1113-7 pubmed
    ..Deletion of the A + T-rich sequences upstream of the E. coli leu operon does not markedly affect the strength of the leu promoter in vivo. ..
  14. Madhusudan S, Paukner A, Klingen Y, Schnetz K. Independent regulation of H-NS-mediated silencing of the bgl operon at two levels: upstream by BglJ and LeuO and downstream by DnaKJ. Microbiology. 2005;151:3349-59 pubmed
    ..disrupting rpoS and crl were obtained, as well as mutations mapping upstream of the open reading frames of bglJ, leuO and dnaK. In rpoS and crl mutants, bgl promoter activity is known to be higher...
  15. Klauck E, Böhringer J, Hengge Aronis R. The LysR-like regulator LeuO in Escherichia coli is involved in the translational regulation of rpoS by affecting the expression of the small regulatory DsrA-RNA. Mol Microbiol. 1997;25:559-69 pubmed
    ..the identification of an additional factor involved in the regulation of rpoS translation, the LysR-like regulator LeuO. Tn10-8 decreases rpoS translation predominantly at low growth temperature...