Gene Symbol: leuD
Description: 3-isopropylmalate dehydratase small subunit
Alias: ECK0073, JW0070
Species: Escherichia coli str. K-12 substr. MG1655
Fultz P, Kemper J. Wild-type isopropylmalate isomerase in Salmonella typhimurium is composed of two different subunits. J Bacteriol. 1981;148:210-9 pubmed
..It is a complex enzyme composed of two subunits which are coded for by two genes of the leucine operon, leuC and leuD. The two polypeptides have been shown to copurify through successive ammonium sulfate fractionations and have been ..
Davis M, Calvo J. Isolation and characterization of lambda pleu bacteriophages. J Bacteriol. 1977;129:1078-90 pubmed
..One type (e.g., lambda pleu9) transduces leuD, leuC, and leuB strains to prototrophy. The other type (e.g...
Somers J, Amzallag A, Middleton R. Genetic fine structure of the leucine operon of Escherichia coli K-12. J Bacteriol. 1973;113:1268-72 pubmed
..1.1). It is concluded that the gross structure of the leucine operon in E. coli is closely similar to, if not identical with, the gross structure of the leucine operon in Salmonella typhimurium. ..
Yang H, Kessler D. Genetic analysis of the leucine region in Escherichia coli B-r: gene-enzyme assignments. J Bacteriol. 1974;117:63-72 pubmed
..These studies have identified four genes in the leucine region having the same order as found in Salmonella typhimurium: ara... leuDCBA. ..
Fultz P, Kwoh D, Kemper J. Salmonella typhimurium newD and Escherichia coli leuC genes code for a functional isopropylmalate isomerase in Salmonella typhimurium-Escherichia coli hybrids. J Bacteriol. 1979;137:1253-62 pubmed
The supQ newD gene substitution system in Salmonella typhimurium restores leucine prototrophy to leuD mutants by providing the newD gene product which is capable of replacing the missing leuD polypeptide in the isopropylmalate isomerase, ..
Hare R, Walker S, Dorman T, Greene J, Guzman L, Kenney T, et al
. Genetic footprinting in bacteria. J Bacteriol. 2001;183:1694-706 pubmed
..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system. ..
Yang C, Shapiro B, Hung S, Mjolsness E, Hatfield G. A mathematical model for the branched chain amino acid biosynthetic pathways of Escherichia coli K12. J Biol Chem. 2005;280:11224-32 pubmed
..This model simulates the results of experimental measurements. ..
Jang S, Imlay J. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. J Biol Chem. 2007;282:929-37 pubmed
..The extreme vulnerability of these enzymes may explain why many organisms, including mammals, deploy H2O2 to suppress microbial growth. ..
Macomber L, Imlay J. The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity. Proc Natl Acad Sci U S A. 2009;106:8344-9 pubmed publisher
..Copper efflux by dedicated export systems, chelation by glutathione, and cluster repair by assembly systems all enhance the resistance of cells to this metal. ..