Gene Symbol: leuC
Description: 3-isopropylmalate dehydratase large subunit
Alias: ECK0074, JW0071
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Davis M, Calvo J. Isolation and characterization of lambda pleu bacteriophages. J Bacteriol. 1977;129:1078-90 pubmed
    ..One type (e.g., lambda pleu9) transduces leuD, leuC, and leuB strains to prototrophy. The other type (e.g., lambda pleu 13) transduces leuA strains to prototrophy...
  2. Chen H, Lawrence C, Bryan S, Moses R. Aphidicolin inhibits DNA polymerase II of Escherichia coli, an alpha-like DNA polymerase. Nucleic Acids Res. 1990;18:7185-6 pubmed
  3. Somers J, Amzallag A, Middleton R. Genetic fine structure of the leucine operon of Escherichia coli K-12. J Bacteriol. 1973;113:1268-72 pubmed
    ..1.1). It is concluded that the gross structure of the leucine operon in E. coli is closely similar to, if not identical with, the gross structure of the leucine operon in Salmonella typhimurium. ..
  4. Yang H, Kessler D. Genetic analysis of the leucine region in Escherichia coli B-r: gene-enzyme assignments. J Bacteriol. 1974;117:63-72 pubmed
    ..These studies have identified four genes in the leucine region having the same order as found in Salmonella typhimurium: ara... leuDCBA. ..
  5. Hare R, Walker S, Dorman T, Greene J, Guzman L, Kenney T, et al. Genetic footprinting in bacteria. J Bacteriol. 2001;183:1694-706 pubmed
    ..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system. ..
  6. Kirino H, Aoki M, Aoshima M, Hayashi Y, Ohba M, Yamagishi A, et al. Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus. Eur J Biochem. 1994;220:275-81 pubmed
    ..Two residues of the E. coli dehydrogenase, Glu256 and Met259, were replaced with the corresponding residues from the thermophile sequence. The resulted mutant enzyme was more resistant to heat than the wild-type enzyme...
  7. Yang C, Shapiro B, Hung S, Mjolsness E, Hatfield G. A mathematical model for the branched chain amino acid biosynthetic pathways of Escherichia coli K12. J Biol Chem. 2005;280:11224-32 pubmed
    ..This model simulates the results of experimental measurements. ..
  8. Jang S, Imlay J. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. J Biol Chem. 2007;282:929-37 pubmed
    ..The extreme vulnerability of these enzymes may explain why many organisms, including mammals, deploy H2O2 to suppress microbial growth. ..
  9. Macomber L, Imlay J. The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity. Proc Natl Acad Sci U S A. 2009;106:8344-9 pubmed publisher
    ..Copper efflux by dedicated export systems, chelation by glutathione, and cluster repair by assembly systems all enhance the resistance of cells to this metal. ..

More Information


  1. Fultz P, Kwoh D, Kemper J. Salmonella typhimurium newD and Escherichia coli leuC genes code for a functional isopropylmalate isomerase in Salmonella typhimurium-Escherichia coli hybrids. J Bacteriol. 1979;137:1253-62 pubmed
    ..which is capable of replacing the missing leuD polypeptide in the isopropylmalate isomerase, a complex of the leuC and leuD gene product. Mutations in the supQ gene are required to make the newD protein available...
  2. Fultz P, Kemper J. Wild-type isopropylmalate isomerase in Salmonella typhimurium is composed of two different subunits. J Bacteriol. 1981;148:210-9 pubmed
    ..It is a complex enzyme composed of two subunits which are coded for by two genes of the leucine operon, leuC and leuD...
  3. Kim Y, Park J, Cho J, Cho K, Park Y, Lee J. Proteomic response analysis of a threonine-overproducing mutant of Escherichia coli. Biochem J. 2004;381:823-9 pubmed
    ..coli mutant. ..