Gene Symbol: leuA
Description: 2-isopropylmalate synthase
Alias: ECK0076, JW0073
Species: Escherichia coli str. K-12 substr. MG1655
Products:     leuA

Top Publications

  1. Chance M, Fiser A, Sali A, Pieper U, Eswar N, Xu G, et al. High-throughput computational and experimental techniques in structural genomics. Genome Res. 2004;14:2145-54 pubmed publisher
    ..This projected resource will provide structural biology information important to understanding the function of most proteins of the cell...
  2. Somers J, Amzallag A, Middleton R. Genetic fine structure of the leucine operon of Escherichia coli K-12. J Bacteriol. 1973;113:1268-72 pubmed
    ..1.1). It is concluded that the gross structure of the leucine operon in E. coli is closely similar to, if not identical with, the gross structure of the leucine operon in Salmonella typhimurium. ..
  3. Wessler S, Calvo J. Control of leu operon expression in Escherichia coli by a transcription attenuation mechanism. J Mol Biol. 1981;149:579-97 pubmed
  4. Gemmill R, Jones J, Haughn G, Calvo J. Transcription initiation sites of the leucine operons of Salmonella typhimurium and Escherichia coli. J Mol Biol. 1983;170:39-59 pubmed
    ..vivo and demonstrate that the site of premature termination is between the promoter and the first structural gene (leuA)...
  5. Chen C, Fang M, Majumder A, Wu H. A 72-base pair AT-rich DNA sequence element functions as a bacterial gene silencer. J Biol Chem. 2001;276:9478-85 pubmed
    ..Furthermore, an increased gene-silencing effect was observed with a tandemly repeated AT4 dimer. The possible mechanism of AT4-mediated gene silencing in bacteria is discussed. ..
  6. Hare R, Walker S, Dorman T, Greene J, Guzman L, Kenney T, et al. Genetic footprinting in bacteria. J Bacteriol. 2001;183:1694-706 pubmed
    ..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system. ..
  7. Calvo J, Kalyanpur M, Stevens C. 2-Isopropylmalate and 3-isopropylmalate as intermediates in leucine biosynthesis. Biochemistry. 1962;1:1157-61 pubmed
  8. Davis M, Calvo J. Isolation and characterization of lambda pleu bacteriophages. J Bacteriol. 1977;129:1078-90 pubmed
    ..none of the enzymes coded for by the leucine operon is synthesized due to an insertion of phage lambda into cistron leuA. The orientation of lambda in the chromosome is ara leuDCB lambda JAN leuA...
  9. Park J, Lee K, Kim T, Lee S. Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation. Proc Natl Acad Sci U S A. 2007;104:7797-802 pubmed
    ..The ilvA, leuA, and panB genes were deleted to make more precursors available for L-valine biosynthesis...

More Information


  1. Fredriksson A, Nystrom T. Conditional and replicative senescence in Escherichia coli. Curr Opin Microbiol. 2006;9:612-8 pubmed
    ..Thus, bacterial physiology might entail both conditional and mandatory aging processes. ..
  2. Fang M, Wu H. A promoter relay mechanism for sequential gene activation. J Bacteriol. 1998;180:626-33 pubmed
    ..This is the first example of several related genes communicating via a promoter relay mechanism for their coordinated expression. ..
  3. Fang M, Wu H. Suppression of leu-500 mutation in topA+ Salmonella typhimurium strains. The promoter relay at work. J Biol Chem. 1998;273:29929-34 pubmed
    ..It appears that the leu-500 suppression in a topA- strain is due to the constant ilvIH transcription activity. ..
  4. Yang C, Shapiro B, Hung S, Mjolsness E, Hatfield G. A mathematical model for the branched chain amino acid biosynthetic pathways of Escherichia coli K12. J Biol Chem. 2005;280:11224-32 pubmed
    ..This model simulates the results of experimental measurements. ..
  5. Soini J, Falschlehner C, Liedert C, Bernhardt J, Vuoristo J, Neubauer P. Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110. Microb Cell Fact. 2008;7:30 pubmed publisher
    ..Therefore we believe that our finding is relevant for recombinant protein production but also may even have implications for the physiology of E. coli under oxygen limitation in general. ..
  6. Genschik P, Billy E, Swianiewicz M, Filipowicz W. The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea. EMBO J. 1997;16:2955-67 pubmed
    ..The conservation of the RNA 3'-terminal phosphate cyclase among Eucarya, Bacteria and Archaea argues that the enzyme performs an important function in RNA metabolism. ..
  7. Stieglitz B, Calvo J. Distribution of the isopropylmalate pathway to leucine among diverse bacteria. J Bacteriol. 1974;118:935-41 pubmed
    ..Taken together with other data, these results suggest that the isopropylmalate pathway is widespread among organisms that can synthesize leucine. ..
  8. Bartkus J, Tyler B, Calvo J. Transcription attenuation-mediated control of leu operon expression: influence of the number of Leu control codons. J Bacteriol. 1991;173:1634-41 pubmed
    ..Given two Leu control codons, the operon responds with nearly maximum output over a wide range of leucine limitation, and that outcome does not change much with increasing numbers of control codons. ..