L arabinose isomerase


Gene Symbol: L arabinose isomerase
Description: L-arabinose isomerase
Alias: ECK0063, JW0061
Species: Escherichia coli str. K-12 substr. MG1655
Products:     L arabinose isomerase

Top Publications

  1. Wallace L, Eiserling F, Wilcox G. The shape of L-arabinose isomerase from Escherichia coli. J Biol Chem. 1978;253:3717-20 pubmed
    ..These measurements are consisting with the interpretation of the profiles as representing two different orientations of the L-arabinose isomerase. ..
  2. De Muynck C, Van der Borght J, De Mey M, De Maeseneire S, Van Bogaert I, Beauprez J, et al. Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli. Appl Microbiol Biotechnol. 2007;76:1051-7 pubmed
    ..Hence, the mutant clones could be screened on a minimal medium with L-ribose as the sole carbon source. Through the screening, two first-generation mutants were isolated, which expressed a small amount of L-ribose isomerase activity. ..
  3. Lee D, Huo L, Schleif R. Repression of the araBAD promoter from araO1. J Mol Biol. 1992;224:335-41 pubmed
  4. Kim P, Yoon S, Roh H, Choi J. High production of D-tagatose, a potential sugar substitute, using immobilized L-arabinose isomerase. Biotechnol Prog. 2001;17:208-10 pubmed
    ..The process was repeated two more times with production of 104.1 and 103.5 g-tagatose/L. D-Tagatose production using an immobilized L-arabinose isomerase has a high potential for commercial application. ..
  5. Patrick J, Lee N. Purification and properties of an L-arabinose isomerase from Escherichia coli. J Biol Chem. 1968;243:4312-8 pubmed
  6. Banerjee S, Anderson F, Farber G. The evolution of sugar isomerases. Protein Eng. 1995;8:1189-95 pubmed
    ..This work also suggests that unless convergent evolution has been demonstrated, the mechanism of one enzyme may not give any insight into the mechanism of a second enzyme catalyzing the same reaction. ..
  7. Reeder T, Schleif R. Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon. J Bacteriol. 1991;173:7765-71 pubmed
    ..S. Naom, S. J. Morton, D. R. F. Leach, and R. G. Lloyd, Nucleic Acids Res. 17:8033-8044, 1989). The carboxyl region of SbcC has an amino acid sequence consistent with this region of SbcC forming an extended alpha-helical coiled-coil. ..
  8. Ibanez E, Gimenez R, Pedraza T, Baldoma L, Aguilar J, Badia J. Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose. J Bacteriol. 2000;182:4625-7 pubmed
    ..Similarity of YiaR to several 3-epimerases suggested that this protein could catalyze the conversion of L-xylulose-5-phosphate into L-ribulose-5-phosphate, thus completing the pathway between L-xylulose and the general metabolism. ..
  9. Lee N, Gielow W, Martin R, Hamilton E, Fowler A. The organization of the araBAD operon of Escherichia coli. Gene. 1986;47:231-44 pubmed
    ..The most pronounced difference, found in the araD gene, is the deletion of a single nt in the S. typhimurium sequence. ..

More Information


  1. Schleif R, Greenblatt J, Davis R. Dual control of arabinose genes on transducing phage lambda-dara. J Mol Biol. 1971;59:127-50 pubmed
  2. Patrick J, Lee N, Barnes N, Englesberg E. Coordination of enzyme synthesis in the L-arabinose operon in Escherichia coli. I. The effect of manganous ion on the synthesis of L-arabinose isomerase. J Biol Chem. 1971;246:5102-6 pubmed
  3. Roh H, Kim P, Park Y, Choi J. Bioconversion of D-galactose into D-tagatose by expression of L-arabinose isomerase. Biotechnol Appl Biochem. 2000;31 ( Pt 1):1-4 pubmed
    ..coli with pTC101, pTC105 and pTC106, tagatose was produced from galactose in 9.9, 7.1 and 6.9% yields respectively. The enzyme extract of E. coli with the plasmid pTC101 also converted galactose into tagatose with a 96.4% yield. ..
  4. Casadaban M. Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions. Proc Natl Acad Sci U S A. 1975;72:809-13 pubmed
    ..These fusions extend the techniques available for studies on the lac operon to studies on the ara operon. It should be possible to fuse other operons by this method. ..
  5. Johnson C, Schleif R. In vivo induction kinetics of the arabinose promoters in Escherichia coli. J Bacteriol. 1995;177:3438-42 pubmed
    ..5, 5, and 1, respectively. These results provide a basis for subsequent studies to determine the mechanism(s) by which AraC protein activates transcription from the different arabinose promoters. ..
  6. Ebright R, Beckwith J. The catabolite gene activator protein (CAP) is not required for indole-3-acetic acid to activate transcription of the araBAD operon of Escherichia coli K-12. Mol Gen Genet. 1985;201:51-5 pubmed
    ..However, contrary to the results of Kline et al., we find that the action of IAA does not require CAP. Thus, IAA fully stimulates the transcription of araBAD in a strain which bears a complete deletion of the crp gene. ..
  7. Wade J, Struhl K. Association of RNA polymerase with transcribed regions in Escherichia coli. Proc Natl Acad Sci U S A. 2004;101:17777-82 pubmed
    ..These observations suggest that in vivo association of sigma(70) and NusA with elongating RNAP is regulated by growth conditions. ..
  8. Manjasetty B, Chance M. Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production. J Mol Biol. 2006;360:297-309 pubmed
    ..Further, the crystal structure of ECAI forms a basis for identifying molecular determinants responsible for isomerization of arabinose to ribulose in vivo and galactose to tagatose in vitro. ..
  9. Schleif R. L-arabinose operon messenger of Escherichia coli. Its inducibility and translation efficiency relative to lactose operon messenger. J Mol Biol. 1971;61:275-9 pubmed
  10. Patrick J, Lee N. Subunit structure of L-arabinose isomerase from Escherichia coli. J Biol Chem. 1969;244:4277-83 pubmed
  11. Lee D, Schleif R. In vivo DNA loops in araCBAD: size limits and helical repeat. Proc Natl Acad Sci U S A. 1989;86:476-80 pubmed
    ..As the spacing between araI and araO2 varied, the activity of PBAD oscillated with an 11.1-base-pair periodicity, implying that the in vivo helical repeat of this DNA is 11.1 base pairs per turn. ..
  12. Gross J, Englesberg E. Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r bv transduction with phage Plbt. Virology. 1959;9:314-31 pubmed