Genomes and Genes
Gene Symbol: kup
Description: potassium transporter
Alias: ECK3741, JW5609, trkD
Species: Escherichia coli str. K-12 substr. MG1655
- Stumpe -, Bakker -. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed..cells without rapid K+-uptake systems did not recover, cells containing either the Trk systems or the overproduced Kup system slowly reversed the effects of protamine, and growth resumed after the cells had reached their original ..
- Bossemeyer D, Borchard A, Dosch D, Helmer G, Epstein W, Booth I, et al. K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane. J Biol Chem. 1989;264:16403-10 pubmed..These results support the model, previously based solely on genetic data, that Trk is a multisubunit complex and implicates the products of the other trk genes in the normal binding of TrkA to the complex in the cytoplasmic membrane...
- Schleyer M, Bakker E. Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus. J Bacteriol. 1993;175:6925-31 pubmedThe kup (formerly trkD) gene from Escherichia coli encodes a minor K(+)-uptake system. The gene is located just upstream of the rbsDACBK operon at 84.5 min on the chromosome and is transcribed clockwise...
- Epstein W, Buurman E, McLaggan D, Naprstek J. Multiple mechanisms, roles and controls of K+ transport in Escherichia coli. Biochem Soc Trans. 1993;21:1006-10 pubmed
- Meury J, Lebail S, Kepes A. Opening of potassium channels in Escherichia coli membranes by thiol reagents and recovery of potassium tightness. Eur J Biochem. 1980;113:33-8 pubmed..a derepressed kdp uptake system working in the micromolar range of the K+ concentration is responsible for the intracellular pool of K+ and when, in a medium millimolar K+ concentration range, the trkA and trkD systems are predominant.
- Epstein W, Kim B. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971;108:639-44 pubmed..The trk loci are widely scattered over the E. coli chromosome; none of them is very near any other trk locus or near the kdp genes. ..
- Trchounian A, Kobayashi H. Kup is the major K+ uptake system in Escherichia coli upon hyper-osmotic stress at a low pH. FEBS Lett. 1999;447:144-8 pubmed..Such a K+ uptake was investigated using a mutant defective in Kdp and TrkA but with a functional Kup and a mutant defective in Kdp and Kup but having an active TrkA...
- Trchounian A, Kobayashi H. K+ uptake by fermenting Escherichia coli cells: pH dependent mode of the TrkA system operating. Biosci Rep. 2000;20:277-88 pubmed..K+ by means of multiple transport systems, of which TrkA is the most prominent at neutral and alkaline pH while Kup is major at acidic pH...
- Bossemeyer D, Schlosser A, Bakker E. Specific cesium transport via the Escherichia coli Kup (TrkD) K+ uptake system. J Bacteriol. 1989;171:2219-21 pubmedEscherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity...
- Rhoads D, Waters F, Epstein W. Cation transport in Escherichia coli. VIII. Potassium transport mutants. J Gen Physiol. 1976;67:325-41 pubmed..This system is repressed by growth in high concentrations of K. Two constitutive systems, TrkA and TrkD, have Km's of 1.5 and 0.5 mM and Vmax's of 550 and 40 at 37 and 30 degrees C, respectively...
- Trchounian A. Ion exchange in facultative anaerobes: does a proton-potassium pump exist in anaerobic Escherichia Coli?. Anaerobe. 1997;3:355-71 pubmed