kup

Summary

Gene Symbol: kup
Description: potassium transporter
Alias: ECK3741, JW5609, trkD
Species: Escherichia coli str. K-12 substr. MG1655
Products:     kup

Top Publications

  1. Rhoads D, Epstein W. Cation transport in Escherichia coli. IX. Regulation of K transport. J Gen Physiol. 1978;72:283-95 pubmed
    ..net K uptake after osmotic upshock are reported for the four K transport systems of Escherichia coli: Kdp, TrkA, TrkD, and TrkF. Energy requirements for K exchange are reported for the Kdp and TrkA systems...
  2. Buurman E, McLaggan D, Naprstek J, Epstein W. Multiple paths for nonphysiological transport of K+ in Escherichia coli. J Bacteriol. 2004;186:4238-45 pubmed
    ..These results support the view that TrkF is composed of multiple, "aberrant" K+ transport activities, i.e., paths that, regardless of their physiological function, allow K+ to cross the cell membrane by a uniport process. ..
  3. Trchounian A, Bagramyan K, Poladian A. Formate hydrogenlyase is needed for proton-potassium exchange through the F0F1-ATPase and the TrkA system in anaerobically grown and glycolysing Escherichia coli. Curr Microbiol. 1997;35:201-6 pubmed
    ..H2 production was observed in the trkA trkD kdpA mutant...
  4. Rhoads D, Woo A, Epstein W. Discrimination between Rb+ and K+ by Escherichia coli. Biochim Biophys Acta. 1977;469:45-51 pubmed
    ..The two moderate affinity systems, the high-rate TrkA system (Km = 1.5 mM) and the moderate rate TrkD system (Km = 0.5 mM), discriminate against a 86Rb+ tracer by approximately 10-fold and 25-fold, respectively...
  5. Kirakosyan G, Bagramyan K, Trchounian A. Redox sensing by Escherichia coli: effects of dithiothreitol, a redox reagent reducing disulphides, on bacterial growth. Biochem Biophys Res Commun. 2004;325:803-6 pubmed
  6. Zakharyan E, Trchounian A. K+ influx by Kup in Escherichia coli is accompanied by a decrease in H+ efflux. FEMS Microbiol Lett. 2001;204:61-4 pubmed
    Escherichia coli accumulates K+ by means of multiple uptake systems of which Kup is the major transport system at acidic pH. In cells grown under fermentative conditions at pH 5...
  7. Trchounian A, Ohanjayan E, Zakharyan E. Regulation of intracellular pH and proton-potassium exchange in fermenting Escherichia coli grown anaerobically in alkaline medium. Membr Cell Biol. 1998;12:67-78 pubmed
    ..with the defect in the alpha-subunit of H(+)-ATPase F0F1 complex (uncA) or in the TrkA system of K+ uptake (trkA trkD)...
  8. Ukai H, Matsuzawa H, Ito K, Yamada M, Nishimura A. ftsE(Ts) affects translocation of K+-pump proteins into the cytoplasmic membrane of Escherichia coli. J Bacteriol. 1998;180:3663-70 pubmed
    ..We expressed alkaline phosphatase (PhoA) fusion proteins of KdpA, Kup, and TrkH, all of which proved functional in vivo as K+ ion pumps, in the mutant cells...
  9. Stumpe S, Bakker E. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed
    ..cells without rapid K+-uptake systems did not recover, cells containing either the Trk systems or the overproduced Kup system slowly reversed the effects of protamine, and growth resumed after the cells had reached their original ..

More Information

Publications20

  1. Stumpe -, Bakker -. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed
    ..cells without rapid K+-uptake systems did not recover, cells containing either the Trk systems or the overproduced Kup system slowly reversed the effects of protamine, and growth resumed after the cells had reached their original ..
  2. Bossemeyer D, Borchard A, Dosch D, Helmer G, Epstein W, Booth I, et al. K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane. J Biol Chem. 1989;264:16403-10 pubmed
    ..These results support the model, previously based solely on genetic data, that Trk is a multisubunit complex and implicates the products of the other trk genes in the normal binding of TrkA to the complex in the cytoplasmic membrane...
  3. Schleyer M, Bakker E. Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus. J Bacteriol. 1993;175:6925-31 pubmed
    The kup (formerly trkD) gene from Escherichia coli encodes a minor K(+)-uptake system. The gene is located just upstream of the rbsDACBK operon at 84.5 min on the chromosome and is transcribed clockwise...
  4. Epstein W, Buurman E, McLaggan D, Naprstek J. Multiple mechanisms, roles and controls of K+ transport in Escherichia coli. Biochem Soc Trans. 1993;21:1006-10 pubmed
  5. Meury J, Lebail S, Kepes A. Opening of potassium channels in Escherichia coli membranes by thiol reagents and recovery of potassium tightness. Eur J Biochem. 1980;113:33-8 pubmed
    ..a derepressed kdp uptake system working in the micromolar range of the K+ concentration is responsible for the intracellular pool of K+ and when, in a medium millimolar K+ concentration range, the trkA and trkD systems are predominant.
  6. Epstein W, Kim B. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971;108:639-44 pubmed
    ..The trk loci are widely scattered over the E. coli chromosome; none of them is very near any other trk locus or near the kdp genes. ..
  7. Trchounian A, Kobayashi H. Kup is the major K+ uptake system in Escherichia coli upon hyper-osmotic stress at a low pH. FEBS Lett. 1999;447:144-8 pubmed
    ..Such a K+ uptake was investigated using a mutant defective in Kdp and TrkA but with a functional Kup and a mutant defective in Kdp and Kup but having an active TrkA...
  8. Trchounian A, Kobayashi H. K+ uptake by fermenting Escherichia coli cells: pH dependent mode of the TrkA system operating. Biosci Rep. 2000;20:277-88 pubmed
    ..K+ by means of multiple transport systems, of which TrkA is the most prominent at neutral and alkaline pH while Kup is major at acidic pH...
  9. Bossemeyer D, Schlosser A, Bakker E. Specific cesium transport via the Escherichia coli Kup (TrkD) K+ uptake system. J Bacteriol. 1989;171:2219-21 pubmed
    Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity...
  10. Rhoads D, Waters F, Epstein W. Cation transport in Escherichia coli. VIII. Potassium transport mutants. J Gen Physiol. 1976;67:325-41 pubmed
    ..This system is repressed by growth in high concentrations of K. Two constitutive systems, TrkA and TrkD, have Km's of 1.5 and 0.5 mM and Vmax's of 550 and 40 at 37 and 30 degrees C, respectively...
  11. Trchounian A. Ion exchange in facultative anaerobes: does a proton-potassium pump exist in anaerobic Escherichia Coli?. Anaerobe. 1997;3:355-71 pubmed