kefC

Summary

Gene Symbol: kefC
Description: potassium:proton antiporter
Alias: ECK0048, JW0046, trkC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Miller S, Douglas R, Carter P, Booth I. Mutations in the glutathione-gated KefC K+ efflux system of Escherichia coli that cause constitutive activation. J Biol Chem. 1997;272:24942-7 pubmed
    The kefC gene of Escherichia coli encodes a potassium efflux system that is gated by glutathione (GSH) and by GSH adducts. Independently isolated kefC mutations that result in spontaneous activation of the efflux system have been analyzed...
  2. Miller S, Ness L, Wood C, Fox B, Booth I. Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. J Bacteriol. 2000;182:6536-40 pubmed
    A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC...
  3. Fujisawa M, Ito M, Krulwich T. Three two-component transporters with channel-like properties have monovalent cation/proton antiport activity. Proc Natl Acad Sci U S A. 2007;104:13289-94 pubmed
    ..are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity...
  4. Ferguson G, Battista J, Lee A, Booth I. Protection of the DNA during the exposure of Escherichia coli cells to a toxic metabolite: the role of the KefB and KefC potassium channels. Mol Microbiol. 2000;35:113-22 pubmed
    ..The activity of the KefB and KefC potassium channels protects E. coli cells against methylglyoxal and limits the amount of DNA damage...
  5. Munro A, Ritchie G, Lamb A, Douglas R, Booth I. The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli. Mol Microbiol. 1991;5:607-16 pubmed
    The kefC gene of Escherichia coli encodes a potassium-efflux system that is regulated by glutathione metabolites. The close proximity of the E...
  6. Elmore M, Lamb A, Ritchie G, Douglas R, Munro A, Gajewska A, et al. Activation of potassium efflux from Escherichia coli by glutathione metabolites. Mol Microbiol. 1990;4:405-12 pubmed
    ..factor is the formation of specific glutathione metabolites that activate transport systems encoded by the kefB and kefC gene products...
  7. Ness L, Booth I. Different foci for the regulation of the activity of the KefB and KefC glutathione-gated K+ efflux systems. J Biol Chem. 1999;274:9524-30 pubmed
    KefB and KefC are glutathione-gated K+ efflux systems in Escherichia coli, and the proteins exhibit strong similarity at the level of both primary sequence and domain organization...
  8. Ferguson G, McLaggan D, Booth I. Potassium channel activation by glutathione-S-conjugates in Escherichia coli: protection against methylglyoxal is mediated by cytoplasmic acidification. Mol Microbiol. 1995;17:1025-33 pubmed
    Escherichia coli possesses two glutathione-gated potassium channels, KefB and KefC, that are activated by glutathione-S-conjugates formed with methylglyoxal...
  9. Reizer J, Reizer A, Saier M. The putative Na+/H+ antiporter (NapA) of Enterococcus hirae is homologous to the putative K+/H+ antiporter (KefC) of Escherichia coli. FEMS Microbiol Lett. 1992;73:161-3 pubmed
    ..ion porters, the putative Na+/H+ antiporter (NapA) of Enterococcus hirae and the putative K+/H+ antiporter (KefC) of Escherichia coli, are similar in sequence throughout their hydrophobic domains...

More Information

Publications24

  1. Roosild T, Castronovo S, Miller S, Li C, Rasmussen T, Bartlett W, et al. KTN (RCK) domains regulate K+ channels and transporters by controlling the dimer-hinge conformation. Structure. 2009;17:893-903 pubmed publisher
    ..Here we report the structure of the C-terminal, KTN-bearing domain of the E. coli KefC K+ efflux system in association with the ancillary subunit, KefF, which is known to stabilize the conductive state...
  2. Ferguson G, Munro A, Douglas R, McLaggan D, Booth I. Activation of potassium channels during metabolite detoxification in Escherichia coli. Mol Microbiol. 1993;9:1297-303 pubmed
    ..g. Escherichia coli, such glutathione adducts activate one, or both, of a pair of potassium efflux systems KefB and KefC. These systems share many of the properties of cation-translocating channels in eukaryotes...
  3. Douglas R, Ritchie G, Munro A, McLaggan D, Booth I. The K(+)-efflux system, KefC, in Escherichia coli: genetic evidence for oligomeric structure. Mol Membr Biol. 1994;11:55-61 pubmed
    b>KefC is a glutathione-gated K(+)-efflux system that is widespread in Gram-negative bacteria and which plays a role in the protection of cells from the toxic effects of electrophilic reagents, such as N-ethylmaleimide (NEM)...
  4. Ferguson G, Creighton R, Nikolaev Y, Booth I. Importance of RpoS and Dps in survival of exposure of both exponential- and stationary-phase Escherichia coli cells to the electrophile N-ethylmaleimide. J Bacteriol. 1998;180:1030-6 pubmed
    ..Stationary-phase E. coli cells were more resistant to NEM than exponential-phase cells. The KefB and KefC systems were found to play an important role in protecting both exponential- and stationary-phase cells against NEM...
  5. Rhoads D, Waters F, Epstein W. Cation transport in Escherichia coli. VIII. Potassium transport mutants. J Gen Physiol. 1976;67:325-41 pubmed
    ..All transport systems are able to extrude Na during K uptake. The measurements of cell Na suggest that growing cells of E. coli have very low concentrations of Na, considerably lower than indicated by earlier studies. ..
  6. Trchounian A. Ion exchange in facultative anaerobes: does a proton-potassium pump exist in anaerobic Escherichia Coli?. Anaerobe. 1997;3:355-71 pubmed
  7. Epstein W, Kim B. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971;108:639-44 pubmed
    ..The trk loci are widely scattered over the E. coli chromosome; none of them is very near any other trk locus or near the kdp genes. ..
  8. Ferguson G, Tötemeyer S, Maclean M, Booth I. Methylglyoxal production in bacteria: suicide or survival?. Arch Microbiol. 1998;170:209-18 pubmed
    ..the primary route of methylglyoxal detoxification, and the glutathione conjugates formed can activate the KefB and KefC potassium channels...
  9. Booth I, Ferguson G, Miller S, Li C, Gunasekera B, Kinghorn S. Bacterial production of methylglyoxal: a survival strategy or death by misadventure?. Biochem Soc Trans. 2003;31:1406-8 pubmed
    ..The KefB and KefC glutathione-gated K(+) efflux systems are integrated with the activity of the glyoxalase system to regulate the ..
  10. Douglas R, Roberts J, Munro A, Ritchie G, Lamb A, Booth I. The distribution of homologues of the Escherichia coli KefC K(+)-efflux system in other bacterial species. J Gen Microbiol. 1991;137:1999-2005 pubmed
    Using a variety of techniques the distribution of the glutathione-regulated KefC K(+)-transport system among bacterial species was investigated. The presence of similar systems in a number of Gram-negative bacteria was demonstrated...
  11. Ferguson G, Nikolaev Y, McLaggan D, MacLean M, Booth I. Survival during exposure to the electrophilic reagent N-ethylmaleimide in Escherichia coli: role of KefB and KefC potassium channels. J Bacteriol. 1997;179:1007-12 pubmed
    The role of the KefB and KefC potassium efflux systems in protecting Escherichia coli cells against the toxic effects of the electrophile N-ethylmaleimide has been investigated...
  12. Booth I, Epstein W, Giffard P, Rowland G. Roles of the trkB and trkC gene products of Escherichia coli in K+ transport. Biochimie. 1985;67:83-9 pubmed
    Mutations at the trkB and trkC loci of Escherichia coli produce an abnormal efflux of K+. The mutations are partially dominant in diploids and revert frequently by what appears to be intragenic suppression to the null state...
  13. Bakker E, Booth I, Dinnbier U, Epstein W, Gajewska A. Evidence for multiple K+ export systems in Escherichia coli. J Bacteriol. 1987;169:3743-9 pubmed
    The role of the K+ transport systems encoded by the kefB (formerly trkB) and kefC (formerly trkC) genes of Escherichia coli in K+ efflux has been investigated...
  14. Smith D, Calvo J. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 1980;8:2255-74 pubmed
    ..A new procedure is described for 3' end labeling of DNA fragments having blunt ends using E. coli exonuclease III and avian myeloblastoma virus reverse transcriptase. ..
  15. Roosild T, Castronovo S, Healy J, Miller S, Pliotas C, Rasmussen T, et al. Mechanism of ligand-gated potassium efflux in bacterial pathogens. Proc Natl Acad Sci U S A. 2010;107:19784-9 pubmed publisher
    ..We have elucidated the mechanism of gating through structural and functional analysis of Escherichia coli KefC. The revealed mechanism can explain how subtle chemical differences in glutathione derivatives can produce opposite ..