kdpD

Summary

Gene Symbol: kdpD
Description: fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein
Alias: ECK0683, JW0683, kac
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Roe A, McLaggan D, O Byrne C, Booth I. Rapid inactivation of the Escherichia coli Kdp K+ uptake system by high potassium concentrations. Mol Microbiol. 2000;35:1235-43 pubmed
  2. Jung K, Krabusch M, Altendorf K. Cs(+) induces the kdp operon of Escherichia coli by lowering the intracellular K(+) concentration. J Bacteriol. 2001;183:3800-3 pubmed
    ..Moreover, the results imply that the signal transduction cascade mediated by KdpD and KdpE is able to integrate multiple signals.
  3. Jung K, Altendorf K. Towards an understanding of the molecular mechanisms of stimulus perception and signal transduction by the KdpD/KdpE system of Escherichia coli. J Mol Microbiol Biotechnol. 2002;4:223-8 pubmed
    The membrane-bound histidine kinase KdpD is a putative turgor sensor that regulates, together with the response regulator KdpE, expression of the kdpFABC operon...
  4. Nakashima K, Sugiura A, Kanamaru K, Mizuno T. Signal transduction between the two regulatory components involved in the regulation of the kdpABC operon in Escherichia coli: phosphorylation-dependent functioning of the positive regulator, KdpE. Mol Microbiol. 1993;7:109-16 pubmed
    The proteins KdpD and KdpE are crucial to the osmotic regulation of the kdpABC operon that is responsible for the high-affinity K+ ion transport system in Escherichia coli...
  5. Heermann R, Altendorf K, Jung K. The N-terminal input domain of the sensor kinase KdpD of Escherichia coli stabilizes the interaction between the cognate response regulator KdpE and the corresponding DNA-binding site. J Biol Chem. 2003;278:51277-84 pubmed
    The sensor kinase/response regulator system KdpD/KdpE of Escherichia coli regulates expression of the kdpFABC operon, which encodes the high affinity K+ transport system KdpFABC...
  6. Jung K, Veen M, Altendorf K. K+ and ionic strength directly influence the autophosphorylation activity of the putative turgor sensor KdpD of Escherichia coli. J Biol Chem. 2000;275:40142-7 pubmed
    The membrane-bound histidine kinase KdpD is a putative turgor sensor that regulates, together with the response regulator KdpE, the expression of the kdpFABC operon coding for the high affinity K(+)-uptake system KdpFABC of Escherichia ..
  7. Jung K, Altendorf K. Truncation of amino acids 12-128 causes deregulation of the phosphatase activity of the sensor kinase KdpD of Escherichia coli. J Biol Chem. 1998;273:17406-10 pubmed
    ..encodes the structural genes for the high affinity K+ transport complex KdpFABC, is regulated by the sensor kinase KdpD and the response regulator KdpE...
  8. Heermann R, Weber A, Mayer B, Ott M, Hauser E, Gabriel G, et al. The universal stress protein UspC scaffolds the KdpD/KdpE signaling cascade of Escherichia coli under salt stress. J Mol Biol. 2009;386:134-48 pubmed publisher
    The sensor kinase KdpD and the response regulator KdpE control induction of the kdpFABC operon encoding the high-affinity K(+)-transport system KdpFABC in response to K(+) limitation or salt stress...
  9. Zimmann P, Puppe W, Altendorf K. Membrane topology analysis of the sensor kinase KdpD of Escherichia coli. J Biol Chem. 1995;270:28282-8 pubmed
    ..kdpFABC operon, coding for the K(+)-translocating Kdp-ATPase, is under the control of the two regulatory proteins KdpD and KdpE, which belong to the group of sensor kinase/response regulator systems...

More Information

Publications54

  1. Facey S, Kuhn A. The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC. Eur J Biochem. 2003;270:1724-34 pubmed
    b>KdpD is a sensor kinase protein in the inner membrane of Escherichia coli containing four transmembrane regions...
  2. Walderhaug M, Polarek J, Voelkner P, Daniel J, Hesse J, Altendorf K, et al. KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators. J Bacteriol. 1992;174:2152-9 pubmed
    ..Expression requires KdpD, a 99-kDa membrane protein, and KdpE, a 25-kDa soluble cytoplasmic protein...
  3. Jung K, Tjaden B, Altendorf K. Purification, reconstitution, and characterization of KdpD, the turgor sensor of Escherichia coli. J Biol Chem. 1997;272:10847-52 pubmed
    In response to K+ availability or medium osmolality, the sensor kinase KdpD and the response regulator KdpE control the expression of the kdpFABC operon, coding for the high affinity K+-translocating Kdp ATPase of Escherichia coli...
  4. Sugiura A, Nakashima K, Tanaka K, Mizuno T. Clarification of the structural and functional features of the osmoregulated kdp operon of Escherichia coli. Mol Microbiol. 1992;6:1769-76 pubmed
    ..e. in addition to them, a cis-acting sequence located upstream of the -35 region was essential for full activation of the promoter. This upstream sequence was demonstrated to be the target site for the trans-acting activator, KdpE. ..
  5. Zimmann P, Steinbrügge A, Schniederberend M, Jung K, Altendorf K. The extension of the fourth transmembrane helix of the sensor kinase KdpD of Escherichia coli is involved in sensing. J Bacteriol. 2007;189:7326-34 pubmed
    The KdpD sensor kinase and the KdpE response regulator control expression of the kdpFABC operon coding for the KdpFABC high-affinity K+ transport system of Escherichia coli...
  6. Brandon L, Dorus S, Epstein W, Altendorf K, Jung K. Modulation of KdpD phosphatase implicated in the physiological expression of the kdp ATPase of Escherichia coli. Mol Microbiol. 2000;38:1086-92 pubmed
    The KdpD sensor kinase and the KdpE response regulator control the expression of the kdpFABC operon, encoding the KdpFABC high-affinity K+ transport system of Escherichia coli...
  7. Jung K, Altendorf K. Individual substitutions of clustered arginine residues of the sensor kinase KdpD of Escherichia coli modulate the ratio of kinase to phosphatase activity. J Biol Chem. 1998;273:26415-20 pubmed
    ..b>KdpD, the sensor kinase of this system, is a bifunctional enzyme catalyzing the autophosphorylation by ATP and the ..
  8. Puppe W, Zimmann P, Jung K, Lucassen M, Altendorf K. Characterization of truncated forms of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coli. J Biol Chem. 1996;271:25027-34 pubmed
    ..of the kdpFABC operon, coding for the K+-translocating Kdp system, is controlled by the two regulatory proteins, KdpD and KdpE, which belong to the group of sensor kinase/response regulator systems...
  9. Voelkner P, Puppe W, Altendorf K. Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coli. Eur J Biochem. 1993;217:1019-26 pubmed
    b>KdpD and KdpE, proteins that control expression of the kdpFABC operon, are members of the class of sensor kinase/response regulator proteins...
  10. Epstein W, Davies M. Potassium-dependant mutants of Escherichia coli K-12. J Bacteriol. 1970;101:836-43 pubmed
    ..1 mm. The mutants do not appear to have a primary alteration in K transport, and are therefore referred to as K-dependent. The abbreviation kdp is proposed for this class of mutant. ..
  11. Ohwada T, Sagisaka S. An immediate and steep increase in ATP concentration in response to reduced turgor pressure in Escherichia coli B. Arch Biochem Biophys. 1987;259:157-63 pubmed
    ..These results indicated that a major fraction of the stress-increased ATP resulted from de novo synthesis, and that it was mainly dependent upon the reaction of substrate-level phosphorylation, which is regulated by turgor pressure. ..
  12. Sugiura A, Hirokawa K, Nakashima K, Mizuno T. Signal-sensing mechanisms of the putative osmosensor KdpD in Escherichia coli. Mol Microbiol. 1994;14:929-38 pubmed
    The KdpD protein is a membrane-located sensory kinase (or signal transducer) critically involved in the regulation of the kdpABC operon that is responsible for a high-affinity transport system in Escherichia coli...
  13. Jung K, Heermann R, Meyer M, Altendorf K. Effect of cysteine replacements on the properties of the turgor sensor KdpD of Escherichia coli. Biochim Biophys Acta. 1998;1372:311-22 pubmed
    ..This process is controlled by the membrane-bound histidine kinase KdpD and the response regulator KdpE...
  14. Rothenbücher M, Facey S, Kiefer D, Kossmann M, Kuhn A. The cytoplasmic C-terminal domain of the Escherichia coli KdpD protein functions as a K+ sensor. J Bacteriol. 2006;188:1950-8 pubmed
    The KdpD protein is a K(+) sensor kinase located in the cytoplasmic membrane of Escherichia coli. It contains four transmembrane stretches and two short periplasmic loops of 4 and 10 amino acid residues, respectively...
  15. Kremling A, Heermann R, Centler F, Jung K, Gilles E. Analysis of two-component signal transduction by mathematical modeling using the KdpD/KdpE system of Escherichia coli. Biosystems. 2004;78:23-37 pubmed
    A mathematical model for the KdpD/KdpE two-component system is presented and its dynamical behavior is analyzed...
  16. Heermann R, Altendorf K, Jung K. The hydrophilic N-terminal domain complements the membrane-anchored C-terminal domain of the sensor kinase KdpD of Escherichia coli. J Biol Chem. 2000;275:17080-5 pubmed
    The putative turgor sensor KdpD is characterized by a large, N-terminal domain of about 400 amino acids, which is not found in any other known sensor kinase...
  17. Heermann R, Fohrmann A, Altendorf K, Jung K. The transmembrane domains of the sensor kinase KdpD of Escherichia coli are not essential for sensing K+ limitation. Mol Microbiol. 2003;47:839-48 pubmed
    The sensor kinase/response regulator system KdpD/KdpE of Escherichia coli regulates the expression of the kdpFABC operon, which encodes the high affinity K+ transport system KdpFABC...
  18. Lüttmann D, Heermann R, Zimmer B, Hillmann A, Rampp I, Jung K, et al. Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli. Mol Microbiol. 2009;72:978-94 pubmed publisher
    ..Transcription initiation at the kdpFABC promoter is positively controlled by the two-component system KdpD/KdpE in response to K+ availability...
  19. Malli R, Epstein W. Expression of the Kdp ATPase is consistent with regulation by turgor pressure. J Bacteriol. 1998;180:5102-8 pubmed
  20. Sardesai A, Gowrishankar J. trans-acting mutations in loci other than kdpDE that affect kdp operon regulation in Escherichia coli: effects of cytoplasmic thiol oxidation status and nucleoid protein H-NS on kdp expression. J Bacteriol. 2001;183:86-93 pubmed
    ..action of a dual-component phosphorelay regulatory system comprised of a sensor kinase (integral membrane protein), KdpD, and a DNA-binding response regulator (cytoplasmic protein), KdpE...
  21. Bramkamp M, Altendorf K, Greie J. Common patterns and unique features of P-type ATPases: a comparative view on the KdpFABC complex from Escherichia coli (Review). Mol Membr Biol. 2007;24:375-86 pubmed
    ..This review compares generic features of P-type ATPases with the rather unique KdpFABC complex and gives a comprehensive overview of common principles of catalysis as well as of special aspects connected to distinct enzyme functions. ..
  22. Frymier J, Reed T, Fletcher S, Csonka L. Characterization of transcriptional regulation of the kdp operon of Salmonella typhimurium. J Bacteriol. 1997;179:3061-3 pubmed
    ..This result, which has not been reported for Escherichia coli, is inconsistent with the model in which the signal for the induction of the kdp operon is turgor loss. ..
  23. Kefala G, Kwiatkowski W, Esquivies L, Maslennikov I, Choe S. Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli. J Struct Funct Genomics. 2007;8:167-72 pubmed
    ..Mistic fusion methods will likely be instrumental for high-level expression of other integral membrane proteins. ..
  24. Maier K, Hubich S, Liebhart H, Krauss S, Kuhn A, Facey S. An amphiphilic region in the cytoplasmic domain of KdpD is recognized by the signal recognition particle and targeted to the Escherichia coli membrane. Mol Microbiol. 2008;68:1471-84 pubmed publisher
    The sensor protein KdpD of Escherichia coli is composed of a large N-terminal hydrophilic region (aa 1-400), four transmembrane regions (aa 401-498) and a large hydrophilic region (aa 499-894) at the C-terminus...
  25. Nakashima K, Sugiura A, Mizuno T. Functional reconstitution of the putative Escherichia coli osmosensor, KdpD, into liposomes. J Biochem. 1993;114:615-21 pubmed
    ..We present the first in vivo evidence that the putative osmosensor (kinase), KdpD, plays a crucial role in the osmotic regulation of kdpABC expression...
  26. Sutherland L, Cairney J, Elmore M, Booth I, Higgins C. Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium. J Bacteriol. 1986;168:805-14 pubmed
    ..The different mechanisms of proU and kdp regulation reflect the different physiological roles of these two osmoprotectants. ..
  27. Heermann R, Jung K. The complexity of the 'simple' two-component system KdpD/KdpE in Escherichia coli. FEMS Microbiol Lett. 2010;304:97-106 pubmed publisher
    The KdpD/KdpE two-component system of Escherichia coli activates the expression of the kdpFABC operon encoding the high-affinity K(+) uptake system KdpFABC in response to K(+) limitation or salt stress...
  28. Nakashima K, Sugiura A, Momoi H, Mizuno T. Phosphotransfer signal transduction between two regulatory factors involved in the osmoregulated kdp operon in Escherichia coli. Mol Microbiol. 1992;6:1777-84 pubmed
    The proteins KdpD and KdpE are regulatory factors critically involved in the osmotic regulation of the kdpABC operon that is responsible for a high-affinity transport system in Escherichia coli...
  29. Trchounian A. Ion exchange in facultative anaerobes: does a proton-potassium pump exist in anaerobic Escherichia Coli?. Anaerobe. 1997;3:355-71 pubmed
  30. Laermann V, udi E, Kipschull K, Zimmann P, Altendorf K. The sensor kinase KdpD of Escherichia coli senses external K+. Mol Microbiol. 2013;88:1194-204 pubmed publisher
    ..of Escherichia coli is composed of the high-affinity K(+) transporter KdpFABC and the two regulatory proteins KdpD (sensor kinase) and KdpE (response regulator), which constitute a typical two-component system...
  31. Hamann K, Zimmann P, Altendorf K. Reduction of turgor is not the stimulus for the sensor kinase KdpD of Escherichia coli. J Bacteriol. 2008;190:2360-7 pubmed publisher
    Stimulus perception by the KdpD/KdpE two-component system of Escherichia coli is still controversial with respect to the nature of the stimulus that is perceived by the sensor kinase KdpD...
  32. Heermann R, Lippert M, Jung K. Domain swapping reveals that the N-terminal domain of the sensor kinase KdpD in Escherichia coli is important for signaling. BMC Microbiol. 2009;9:133 pubmed publisher
    The KdpD/KdpE two-component system of Escherichia coli regulates expression of the kdpFABC operon encoding the high affinity K+ transport system KdpFABC...
  33. Rhoads D, Laimins L, Epstein W. Functional organization of the kdp genes of Escherichia coli K-12. J Bacteriol. 1978;135:445-52 pubmed
    ..The kdpD gene is a separate transcription unit and is the site of mutations making expression of the kdp genes partially ..
  34. Burmeister M. Chromosomal Location of a Gene Involved in Potassium Ion Uptake in Escherichia coli B. J Bacteriol. 1969;100:796-802 pubmed
    ..The mutation, kac-1, is in strain RD-2, which is deficient in K(+) intake, exchanges cell K(+) for extracellular isotope at a reduced ..
  35. Gowrishankar J. Identification of osmoresponsive genes in Escherichia coli: evidence for participation of potassium and proline transport systems in osmoregulation. J Bacteriol. 1985;164:434-45 pubmed
  36. Stallkamp I, Altendorf K, Jung K. Amino acid replacements in transmembrane domain 1 influence osmosensing but not K+ sensing by the sensor kinase KdpD of Escherichia coli. Arch Microbiol. 2002;178:525-30 pubmed
    ..This process is controlled by the sensor kinase/response regulator system KdpD/KdpE...
  37. Stallkamp I, Dowhan W, Altendorf K, Jung K. Negatively charged phospholipids influence the activity of the sensor kinase KdpD of Escherichia coli. Arch Microbiol. 1999;172:295-302 pubmed
    ..Kdp-ATPase of Escherichia coli, encoded by the kdpFABC operon, is regulated by the membrane-bound sensor kinase KdpD and the soluble response regulator KdpE...
  38. Heermann R, Altendorf K, Jung K. The turgor sensor KdpD of Escherichia coli is a homodimer. Biochim Biophys Acta. 1998;1415:114-24 pubmed
    ..Expression of the corresponding operon is controlled by the membrane-bound sensor kinase KdpD and the cytoplasmic response regulator KdpE. Here, we examine the oligomeric state of KdpD...
  39. Asha H, Gowrishankar J. Regulation of kdp operon expression in Escherichia coli: evidence against turgor as signal for transcriptional control. J Bacteriol. 1993;175:4528-37 pubmed
    ..in Escherichia coli, is encoded by genes of the kdpABC operon, and its expression is regulated by the products of kdpD and kdpE. Loss of cell turgor has been proposed to be the signal which induces kdp expression (L. A. Laimins, D. B...
  40. Polarek J, Williams G, Epstein W. The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli. J Bacteriol. 1992;174:2145-51 pubmed
    The expression of the Kdp system for K+ uptake in Escherichia coli requires the products of two genes, kdpD and kdpE. These genes constitute an operon adjacent to the kdpABC operon that encodes the three membrane protein subunits of Kdp...
  41. Ballal A, Heermann R, Jung K, Gassel M, Apte K, Altendorf K. A chimeric Anabaena/ Escherichia coli KdpD protein (Anacoli KdpD) functionally interacts with E. coli KdpE and activates kdp expression in E. coli. Arch Microbiol. 2002;178:141-8 pubmed
    ..Expression of the operon is regulated by the membrane-bound sensor kinase KdpD and the cytosolic response regulator KdpE. From a nitrogen-fixing cyanobacterium, Anabaena sp...
  42. Altendorf K, Voelkner P, Puppe W. The sensor kinase KdpD and the response regulator KdpE control expression of the kdpFABC operon in Escherichia coli. Res Microbiol. 1994;145:374-81 pubmed
  43. Laimins L, Rhoads D, Epstein W. Osmotic control of kdp operon expression in Escherichia coli. Proc Natl Acad Sci U S A. 1981;78:464-8 pubmed
    ..The kdp operon thus appears to be controlled by changes in a physical force, the turgor pressure. ..
  44. Schniederberend M, Zimmann P, Bogdanov M, Dowhan W, Altendorf K. Influence of K+-dependent membrane lipid composition on the expression of the kdpFABC operon in Escherichia coli. Biochim Biophys Acta. 2010;1798:32-9 pubmed publisher
    The membrane-bound sensor kinase KdpD and the cytoplasmic response regulator KdpE regulate the expression of the kdpFABC operon coding for the high affinity potassium uptake system KdpFABC in Escherichia coli...
  45. Maslennikov I, Klammt C, Hwang E, Kefala G, Okamura M, Esquivies L, et al. Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis. Proc Natl Acad Sci U S A. 2010;107:10902-7 pubmed publisher
    ..The ArcB and QseC TM domains are both two-helical motifs, whereas the KdpD TM domain comprises a four-helical bundle with shorter second and third helices...