Gene Symbol: ispG
Description: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Species: Escherichia coli O157:H7 str. Sakai
Seemann M, Bui B, Wolff M, Tritsch D, Campos N, Boronat A, et al
. Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein. Angew Chem Int Ed Engl. 2002;41:4337-9 pubmed
McAteer S, Coulson A, McLennan N, Masters M. The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis. J Bacteriol. 2001;183:7403-7 pubmed publisher
LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis...
Hecht S, Eisenreich W, Adam P, Amslinger S, Kis K, Bacher A, et al
. Studies on the nonmevalonate pathway to terpenes: the role of the GcpE (IspG) protein. Proc Natl Acad Sci U S A. 2001;98:14837-42 pubmed publisher
..The additional expression of the gcpE gene afforded 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as an intermediate of the nonmevalonate pathway...
Rohdich F, Zepeck F, Adam P, Hecht S, Kaiser J, Laupitz R, et al
. The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: studies on the mechanisms of the reactions catalyzed by IspG and IspH protein. Proc Natl Acad Sci U S A. 2003;100:1586-91 pubmed publisher
Earlier in vivo studies have shown that the sequential action of the IspG and IspH proteins is essential for the reductive transformation of 2C-methyl-d-erythritol 2,4-cyclodiphosphate into dimethylallyl diphosphate and isopentenyl ..
Sauret G eto S, Ramos Valdivia A, Ib ez E, Boronat A, Rodr guez Concepci n M. Identification of lethal mutations in Escherichia coli genes encoding enzymes of the methylerythritol phosphate pathway. Biochem Biophys Res Commun. 2003;307:408-15 pubmed
..Besides confirming the role of residues involved in catalysis, our results define regions within several of the proteins with a potential key role for enzyme function...
Baker J, Parker J. Sequence and characterization of the Escherichia coli genome between the ndk and gcpE genes. FEMS Microbiol Lett. 1994;121:293-6 pubmed
The region of the chromosome immediately upstream of the Escherichia coli gene gcpE has been cloned and sequenced...
Rather P, Solinsky K, Paradise M, Parojcic M. aarC, an essential gene involved in density-dependent regulation of the 2'-N-acetyltransferase in Providencia stuartii. J Bacteriol. 1997;179:2267-73 pubmed
..The predicted AarC protein exhibited 88% amino acid homology to the previously identified GcpE protein of Escherichia coli and 86% homology to a gene product from Haemophilus influenzae. The E...
Altincicek B, Moll J, Campos N, Foerster G, Beck E, Hoeffler J, et al
. Cutting edge: human gamma delta T cells are activated by intermediates of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis. J Immunol. 2001;166:3655-8 pubmed
..coli extracts to stimulate gamma delta T cell proliferation is abrogated when genes coding for essential enzymes of the MEP pathway, dxr or gcpE, are disrupted or deleted from the bacterial genome.
Hintz M, Reichenberg A, Altincicek B, Bahr U, Gschwind R, Kollas A, et al
. Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli. FEBS Lett. 2001;509:317-22 pubmed
The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-D-erythritol 4-phosphate pathway in Escherichia coli...