Gene Symbol: ispG
Description: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Alias: gcpE
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Seemann M, Bui B, Wolff M, Tritsch D, Campos N, Boronat A, et al. Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein. Angew Chem Int Ed Engl. 2002;41:4337-9 pubmed
  2. McAteer S, Coulson A, McLennan N, Masters M. The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis. J Bacteriol. 2001;183:7403-7 pubmed publisher
    LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis...
  3. Hecht S, Eisenreich W, Adam P, Amslinger S, Kis K, Bacher A, et al. Studies on the nonmevalonate pathway to terpenes: the role of the GcpE (IspG) protein. Proc Natl Acad Sci U S A. 2001;98:14837-42 pubmed publisher
    ..The additional expression of the gcpE gene afforded 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as an intermediate of the nonmevalonate pathway...
  4. Rohdich F, Zepeck F, Adam P, Hecht S, Kaiser J, Laupitz R, et al. The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: studies on the mechanisms of the reactions catalyzed by IspG and IspH protein. Proc Natl Acad Sci U S A. 2003;100:1586-91 pubmed publisher
    Earlier in vivo studies have shown that the sequential action of the IspG and IspH proteins is essential for the reductive transformation of 2C-methyl-d-erythritol 2,4-cyclodiphosphate into dimethylallyl diphosphate and isopentenyl ..
  5. Sauret G eto S, Ramos Valdivia A, Ib ez E, Boronat A, Rodr guez Concepci n M. Identification of lethal mutations in Escherichia coli genes encoding enzymes of the methylerythritol phosphate pathway. Biochem Biophys Res Commun. 2003;307:408-15 pubmed
    ..Besides confirming the role of residues involved in catalysis, our results define regions within several of the proteins with a potential key role for enzyme function...
  6. Baker J, Parker J. Sequence and characterization of the Escherichia coli genome between the ndk and gcpE genes. FEMS Microbiol Lett. 1994;121:293-6 pubmed
    The region of the chromosome immediately upstream of the Escherichia coli gene gcpE has been cloned and sequenced...
  7. Rather P, Solinsky K, Paradise M, Parojcic M. aarC, an essential gene involved in density-dependent regulation of the 2'-N-acetyltransferase in Providencia stuartii. J Bacteriol. 1997;179:2267-73 pubmed
    ..The predicted AarC protein exhibited 88% amino acid homology to the previously identified GcpE protein of Escherichia coli and 86% homology to a gene product from Haemophilus influenzae. The E...
  8. Altincicek B, Moll J, Campos N, Foerster G, Beck E, Hoeffler J, et al. Cutting edge: human gamma delta T cells are activated by intermediates of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis. J Immunol. 2001;166:3655-8 pubmed
    ..coli extracts to stimulate gamma delta T cell proliferation is abrogated when genes coding for essential enzymes of the MEP pathway, dxr or gcpE, are disrupted or deleted from the bacterial genome.
  9. Hintz M, Reichenberg A, Altincicek B, Bahr U, Gschwind R, Kollas A, et al. Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli. FEBS Lett. 2001;509:317-22 pubmed
    The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-D-erythritol 4-phosphate pathway in Escherichia coli...

More Information


  1. Querol J, Campos N, Imperial S, Boronat A, Rodr guez Concepci n M. Functional analysis of the Arabidopsis thaliana GCPE protein involved in plastid isoprenoid biosynthesis. FEBS Lett. 2002;514:343-6 pubmed
    ..after its discovery, most of the Escherichia coli genes involved in the pathway have been identified, including gcpE. In this work, we have identified an Arabidopsis thaliana protein with homology to the product of this gene...
  2. Campos N, Rodríguez Concepción M, Seemann M, Rohmer M, Boronat A. Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli. FEBS Lett. 2001;488:170-3 pubmed
    ..Using bioinformatic and genetic approaches, we have identified gcpE as a novel gene of the MEP pathway...
  3. Baker J, Franklin D, Parker J. Sequence and characterization of the gcpE gene of Escherichia coli. FEMS Microbiol Lett. 1992;73:175-80 pubmed
    In Escherichia coli, the gene gcpE encodes a protein of unknown function and lies immediately upstream of the hisS gene for histidyl-tRNA synthetase...
  4. Altincicek B, Kollas A, Sanderbrand S, Wiesner J, Hintz M, Beck E, et al. GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli. J Bacteriol. 2001;183:2411-6 pubmed
    ..In this work, we demonstrate that the gcpE gene of Escherichia coli is involved in this pathway. E...