Gene Symbol: ilvM
Description: acetolactate synthase 2 small subunit
Alias: ECK3761, JW3742
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Guardiola J, De Felice M, Lamberti A, Iaccarino M. The acetolactate synthase isoenzymes of Escherichia coli K-12. Mol Gen Genet. 1977;156:17-25 pubmed
  2. Sameshima J, Wek R, Hatfield G. Overlapping transcription and termination of the convergent ilvA and ilvY genes of Escherichia coli. J Biol Chem. 1989;264:1224-31 pubmed
    ..These experiments demonstrate that the converging transcripts from the ilvA and ilvY genes terminate in the coding region of the opposing gene. ..
  3. Hill C, Pang S, Duggleby R. Purification of Escherichia coli acetohydroxyacid synthase isoenzyme II and reconstitution of active enzyme from its individual pure subunits. Biochem J. 1997;327 ( Pt 3):891-8 pubmed
    ..isoenzymes and the present study concerns isoenzyme II, whose large and small subunits are encoded by the ilvG and ilvM genes respectively. Cloning these genes into a plasmid vector and overexpression in E...
  4. Pagel J, Winkelman J, Adams C, Hatfield G. DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli. J Mol Biol. 1992;224:919-35 pubmed
  5. Vyazmensky M, Zherdev Y, Slutzker A, Belenky I, Kryukov O, Barak Z, et al. Interactions between large and small subunits of different acetohydroxyacid synthase isozymes of Escherichia coli. Biochemistry. 2009;48:8731-7 pubmed publisher
    ..nonspecific interaction possible between LSUs and SSUs of different isozymes? To our surprise, the AHAS II SSU ilvM is able to activate the LSUs of all three of the isozymes, and the truncated AHAS III SSUs ilvH-Delta80, ilvH-..
  6. Bar Ilan A, Balan V, Tittmann K, Golbik R, Vyazmensky M, Hübner G, et al. Binding and activation of thiamin diphosphate in acetohydroxyacid synthase. Biochemistry. 2001;40:11946-54 pubmed
    ..The biosynthetic activity of AHAS also involves many other factors beyond the binding and deprotonation of ThDP; changes in the ligands to ThDP can have interesting and unexpected effects on the reaction. ..
  7. Ibdah M, Bar Ilan A, Livnah O, Schloss J, Barak Z, Chipman D. Homology modeling of the structure of bacterial acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis. Biochemistry. 1996;35:16282-91 pubmed
    ..A role for the C terminus of the enzyme polypeptide in determination on the reaction pathway is also possible. ..
  8. LaRossa R, Schloss J. The sulfonylurea herbicide sulfometuron methyl is an extremely potent and selective inhibitor of acetolactate synthase in Salmonella typhimurium. J Biol Chem. 1984;259:8753-7 pubmed
    ..They have acetolactate synthase activity that is insensitive to sulfometuron methyl because of mutations in or near ilvG, the structural gene for acetolactate synthase isozyme II. ..
  9. Dailey F, Cronan J. Acetohydroxy acid synthase I, a required enzyme for isoleucine and valine biosynthesis in Escherichia coli K-12 during growth on acetate as the sole carbon source. J Bacteriol. 1986;165:453-60 pubmed
    ..AHAS III was expressed during growth on acetate or oleate but was somehow unable to produce sufficient amounts of alpha-aceto-alpha-hydroxybutyrate and alpha-acetolactate to allow growth. ..

More Information


  1. Lu M, Umbarger H. Effects of deletion and insertion mutations in the ilvM gene of Escherichia coli. J Bacteriol. 1987;169:600-4 pubmed
    A plasmid was constructed that carried the ilvG and ilvM genes and the associated promoter and leader regions derived from the K-12 strain of Escherichia coli...
  2. Lopes J, Lawther R. Analysis and comparison of the internal promoter, pE, of the ilvGMEDA operons from Escherichia coli K-12 and Salmonella typhimurium. Nucleic Acids Res. 1986;14:2779-98 pubmed
    ..coli K-12 and S. typhimurium; pE is located in the distal end of the ilvM coding sequence; the pE sequence is highly conserved in the two bacteria; the amino acid sequence of the ilvM gene ..
  3. Wek R, Sameshima J, Hatfield G. Rho-dependent transcriptional polarity in the ilvGMEDA operon of wild-type Escherichia coli K12. J Biol Chem. 1987;262:15256-61 pubmed
    ..These transcriptional experiments provide in vitro confirmation for the latent rho-dependent termination site model of transcriptional polarity. ..
  4. Wek R, Hatfield G. Examination of the internal promoter, PE, in the ilvGMEDA operon of E. coli K-12. Nucleic Acids Res. 1986;14:2763-77 pubmed
    The ilvGMEDA operon of Escherichia coli K-12 contains an internal promoter, PE, in the distal portion of the ilvM gene immediately upstream from the ilvE gene...
  5. Cox J, Cox B, Fidanza V, Calhoun D. The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12. Gene. 1987;56:185-98 pubmed
    ..coli. The sequence at the boundary of the ilvA and ilvD genes is TAATAATG, so that the second TAA stop codon of ilvD overlaps the ATG initiation codon of ilvA. ..
  6. Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H. Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence. J Biochem. 1985;97:993-9 pubmed
    ..C., et al. (1979) J. Bacteriol. 139, 339-345). From the deduced amino acid sequence, the secondary structure was predicted...
  7. Parekh B, Hatfield G. Growth rate-related regulation of the ilvGMEDA operon of Escherichia coli K-12 is a consequence of the polar frameshift mutation in the ilvG gene of this strain. J Bacteriol. 1997;179:2086-8 pubmed
    ..Thus, the growth rate-related expression of the ilvGMEDA operon in E. coli K-12 is the fortuitous consequence of the polar frameshift mutation in the ilvG gene of this strain. ..
  8. Engel S, Vyazmensky M, Geresh S, Barak Z, Chipman D. Acetohydroxyacid synthase: a new enzyme for chiral synthesis of R-phenylacetylcarbinol. Biotechnol Bioeng. 2003;83:833-40 pubmed
    ..Acetohydroxyacid synthase isozyme I is particularly efficient. The reaction is not limited to condensation of pyruvate with benzaldehyde and other aromatic aldehydes may be used. ..
  9. Vinogradov M, Kaplun A, Vyazmensky M, Engel S, Golbik R, Tittmann K, et al. Monitoring the acetohydroxy acid synthase reaction and related carboligations by circular dichroism spectroscopy. Anal Biochem. 2005;342:126-33 pubmed
    ..This enzyme catalyzes the condensation of two molecules of glyoxylate to chiral tartronic acid semialdehyde. The use of circular dichroism also identifies the product of glyoxylate carboligase as being in the (R) configuration. ..
  10. Huang F, Coppola G, Calhoun D. Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12. J Bacteriol. 1992;174:4871-7 pubmed
  11. Lawther R, Wek R, Lopes J, Pereira R, Taillon B, Hatfield G. The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 1987;15:2137-55 pubmed
    ..This new information contributes to a more complete understanding of the overall control of the biosynthesis of isoleucine and valine...
  12. Schloss J, Van Dyk D, Vasta J, Kutny R. Purification and properties of Salmonella typhimurium acetolactate synthase isozyme II from Escherichia coli HB101/pDU9. Biochemistry. 1985;24:4952-9 pubmed
    ..3 kDa), purified acetolactate synthase contained a smaller subunit (9.7 kDa; designated here as the ilvM gene product)...
  13. Driver R, Lawther R. Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12. J Bacteriol. 1985;162:598-606 pubmed
    ..This genomic analysis demonstrates that the ilv restriction site map that was established previously by the analysis of recombinant bacteriophage and plasmids is identical to that on the genome. ..
  14. Grant G. The ACT domain: a small molecule binding domain and its role as a common regulatory element. J Biol Chem. 2006;281:33825-9 pubmed