Gene Symbol: ilvE
Description: branched-chain amino acid aminotransferase
Alias: ECK3762, JW5606
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Lawther R, Wek R, Lopes J, Pereira R, Taillon B, Hatfield G. The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 1987;15:2137-55 pubmed
    ..This new information contributes to a more complete understanding of the overall control of the biosynthesis of isoleucine and valine...
  2. Lawther R, Nichols B, Zurawski G, Hatfield G. The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12. Nucleic Acids Res. 1979;7:2289-301 pubmed
    The DNA sequence of the 570 base pairs that precedes and includes the beginning of the ilvE gene shows no evidence of a leader-attenuator region...
  3. Lopes J, Soliman N, Smith P, Lawther R. Transcriptional polarity enhances the contribution of the internal promoter, ilvEp, in the expression of the ilvGMEDA operon in wild-type Escherichia coli K12. Mol Microbiol. 1989;3:1039-51 pubmed
    ..typhimurium. This analysis suggests that ilvEp contributes as much as 90% of ilvEDA expression in wild-type E. coli K12 and only 15% in wild-type S. typhimurium when grown under non-repressing conditions. ..
  4. Uzan M, Favre R, Gallay E, Caro L. Genetical and structural analysis of a group of lambda ilv and lambda rho transducing phages. Mol Gen Genet. 1981;182:462-70 pubmed
    ..Finally, we have found that one of the lambda ilv phages does not have the classical structure of a transducing phage. ..
  5. Inoue K, Kuramitsu S, Aki K, Watanabe Y, Takagi T, Nishigai M, et al. Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties. J Biochem. 1988;104:777-84 pubmed
    b>ilvE gene of Escherichia coli was inserted into the region downstream of the tac promotor. As a result, the branched-chain amino acid aminotransferase was overproduced by about a hundred-fold in E. coli W3110...
  6. Berg C, Wang M, Vartak N, Liu L. Acquisition of new metabolic capabilities: multicopy suppression by cloned transaminase genes in Escherichia coli K-12. Gene. 1988;65:195-202 pubmed
  7. Driver R, Lawther R. Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12. J Bacteriol. 1985;162:598-606 pubmed
    ..This genomic analysis demonstrates that the ilv restriction site map that was established previously by the analysis of recombinant bacteriophage and plasmids is identical to that on the genome. ..
  8. Subrahmanyam C, Noti J, Umbarger H. Regulation of ilvEDA expression occurs upstream of ilvG in Escherichia coli: additional evidence for an ilvGEDA operon. J Bacteriol. 1980;144:279-90 pubmed
    ..These experiments indicate that ilv-specific control over ilvE, ilvD, and ilvA expression is dependent on these genes being continguous with deoxyribonucleic acid that lies ..
  9. Wek R, Hatfield G. Examination of the internal promoter, PE, in the ilvGMEDA operon of E. coli K-12. Nucleic Acids Res. 1986;14:2763-77 pubmed
    ..coli K-12 contains an internal promoter, PE, in the distal portion of the ilvM gene immediately upstream from the ilvE gene...

More Information


  1. Parekh B, Hatfield G. Growth rate-related regulation of the ilvGMEDA operon of Escherichia coli K-12 is a consequence of the polar frameshift mutation in the ilvG gene of this strain. J Bacteriol. 1997;179:2086-8 pubmed
    In Escherichia coli K-12 the intracellular levels of threonine deaminase and transaminase B, products of ilvA and ilvE, respectively, in the ilvGMEDA operon, increase with increasing growth rates (S. Pedersen, P. L. Bloch, S. Reeh, and F...
  2. Kline E, Manross D, Warwick M. Multivalent regulation of isoleucine-valine transaminase in an Escherichia coli K-12 ilvA deletion strain. J Bacteriol. 1977;130:951-3 pubmed
    ..This activity was due to transaminase B, specified by the ilvE structural gene.
  3. Goto M, Miyahara I, Hayashi H, Kagamiyama H, Hirotsu K. Crystal structures of branched-chain amino acid aminotransferase complexed with glutamate and glutarate: true reaction intermediate and double substrate recognition of the enzyme. Biochemistry. 2003;42:3725-33 pubmed publisher
    ..In the histidinol-phosphate aminotransferase, the acidic and basic side chains are located at different sites and interact with different residues of the disordered loop...
  4. Adelberg E, Umbarger H. Isoleucine and valine metabolism in Escherichia coli. V. alpha-Ketoisovaleric acid accumulation. J Biol Chem. 1953;205:475-82 pubmed
  5. Baez M, Patin D, Calhoun D. Deletion mapping of the ilvGOEDAC genes of Escherichia coli K-12. Mol Gen Genet. 1979;169:289-97 pubmed
    ..Thus, the ilvO gene, that is identified by cis-dominant regulatory mutations that result in increased ilvG and ilvEDA expression, is situated between and may be contiguous with ilvG and ilvEDA. ..
  6. Okada K, Hirotsu K, Sato M, Hayashi H, Kagamiyama H. Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5 A resolution. J Biochem. 1997;121:637-41 pubmed
    ..Although the main chain conformation of the active site is homologous to that of D-amino acid aminotransferase, many of the active site residues are different between them. ..
  7. Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H. Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence. J Biochem. 1985;97:993-9 pubmed
    The ilvE gene of the Escherichia coli K-12 ilvGEDA operon, which encodes branched-chain amino acid aminotransferase [EC], was cloned. The nucleotide sequence of 1.5 kilobase pairs containing the gene was determined...
  8. Rudman D, Meister A. Transamination in Escherichia coli. J Biol Chem. 1953;200:591-604 pubmed
  9. Cox J, Cox B, Fidanza V, Calhoun D. The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12. Gene. 1987;56:185-98 pubmed
    ..coli. The sequence at the boundary of the ilvA and ilvD genes is TAATAATG, so that the second TAA stop codon of ilvD overlaps the ATG initiation codon of ilvA. ..
  10. Huang F, Coppola G, Calhoun D. Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12. J Bacteriol. 1992;174:4871-7 pubmed
    ..6 and 1.1 kb that correspond to the products of the ilvEDA and ilvE genes and two relatively unstable transcripts of 6.7 and 3...
  11. Pagel J, Winkelman J, Adams C, Hatfield G. DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli. J Mol Biol. 1992;224:919-35 pubmed
  12. Jackson J, Henderson E. Mutation affecting regulation of synthesis of acetohydroxy acid synthetase in Escherichia coli K-12. J Bacteriol. 1975;121:504-10 pubmed
    ..Evidence is presented that suggests the presence of multiple genetic regions for synthesis and control of the valine-sensitive AHAS activity. ..
  13. Lee Peng F, Hermodson M, Kohlhaw G. Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity. J Bacteriol. 1979;139:339-45 pubmed
    Transaminase B (branched-chain amino acid aminotransferase, EC, the ilvE gene product, was purified to apparent homogeneity from an Escherichia coli K-12 strain which carries the ilvE gene both on the host chromosome and on a ..
  14. Gelfand D, Steinberg R. Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J Bacteriol. 1977;130:429-40 pubmed
    ..In ilvE- strains, which lack the branched-chain amino acid aminotransferase, the presence of either the tyrosine-..
  15. Wek R, Sameshima J, Hatfield G. Rho-dependent transcriptional polarity in the ilvGMEDA operon of wild-type Escherichia coli K12. J Biol Chem. 1987;262:15256-61 pubmed
    ..These transcriptional experiments provide in vitro confirmation for the latent rho-dependent termination site model of transcriptional polarity. ..
  16. Calhoun D, Wallen J, Traub L, Gray J, Kung H. Internal promoter in the ilvGEDA transcription unit of Escherichia coli K-12. J Bacteriol. 1985;161:128-32 pubmed
    ..pG, preceding ilvG; (ii) the relative activity of pE promoter, previously postulated to be located between ilvG and ilvE; and (iii) the effect of the frameshift site present in the wild-type ilvG gene by comparison with mutant ..
  17. Sameshima J, Wek R, Hatfield G. Overlapping transcription and termination of the convergent ilvA and ilvY genes of Escherichia coli. J Biol Chem. 1989;264:1224-31 pubmed
    ..These experiments demonstrate that the converging transcripts from the ilvA and ilvY genes terminate in the coding region of the opposing gene. ..
  18. Berg C, Shaw K, Berg D. The ilvG gene is expressed in Escherichia coli K-12. Gene. 1980;12:165-70 pubmed
    ..We hypothesize that ilvO- mutations are ilvG frameshift mutations which render AHAS II valine resistant and enhance transcription of distal genes. ..
  19. Gray J, Bennett D, Umbarger H, Calhoun D. Physical and genetic localization of ilv regulatory sites in lambda ilv bacteriophages. J Bacteriol. 1982;149:1071-81 pubmed
    ..gene and, in addition, bacterial DNA that extends to a termination point between ilvA and ilvC, within ilvD, within ilvE, or within ilvG...
  20. Adams C, Lawther R, Hatfield G. The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity. Biochem Biophys Res Commun. 1979;89:650-8 pubmed
  21. Yang C, Shapiro B, Hung S, Mjolsness E, Hatfield G. A mathematical model for the branched chain amino acid biosynthetic pathways of Escherichia coli K12. J Biol Chem. 2005;280:11224-32 pubmed
    ..This model simulates the results of experimental measurements. ..
  22. Asada K, Nakatani S, Takanami M. Cloning of the contiguous 165-kilobase-pair region around the terminus of Escherichia coli K-12 DNA replication. J Bacteriol. 1985;163:398-400 pubmed
    ..The cloned area spanned about 165 kilobase pairs, corresponding to the 29.7-to-33.2-min region of the genetic map of the E. coli chromosome. ..
  23. Xian M, Alaux S, Sagot E, Gefflaut T. Chemoenzymatic synthesis of glutamic acid analogues: substrate specificity and synthetic applications of branched chain aminotransferase from Escherichia coli. J Org Chem. 2007;72:7560-6 pubmed
    ..The usefulness of BCAT was demonstrated through the synthesis of several 3- and 4-substituted derivatives. ..