Gene Symbol: iap
Description: aminopeptidase in alkaline phosphatase isozyme conversion
Alias: ECK2748, JW2723
Species: Escherichia coli str. K-12 substr. MG1655
Products:     iap

Top Publications

  1. Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol. 1987;169:5429-33 pubmed
    The iap gene in Escherichia coli is responsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions...
  2. Nakata A, Amemura M, Yamaguchi M, Izutani K. Factors affecting the formation of alkaline phosphatase isozymes in Escherichia coli K-12. Biken J. 1977;20:47-55 pubmed
    ..coli genetic map. We tentatively proposed to designate this the iap gene(s), an abbreviation for isozyme of alkaline phosphatase, which plays a role in isozyme formation.
  3. Nakata A, Yamaguchi M, Izutani K, Amemura M. Escherichia coli mutants deficient in the production of alkaline phosphatase isozymes. J Bacteriol. 1978;134:287-94 pubmed
    ..The mutation responsible for the altered isozyme pattern, designated iap, was mapped by P1 transduction in the interval between cysC and srl (at about 58.5 min on the E. coli genetic map)...
  4. Nakata A, Shinagawa H, Kawamata J. Inhibition of alkaline phosphatase isozyme conversion by protease inhibitors in Escherichia coli K-12. FEBS Lett. 1979;105:147-50 pubmed
  5. Nakata A, Kawamata J. Dominance of alkaline phosphatase isozyme gene (IAP+) of Escherichia coli K-12. Biken J. 1980;23:49-51 pubmed
    A mutation (iap-) causing a defect in the conversion of alkaline phosphatase isozymes of Escherichia coli K-12 is recessive to the wild type allele. The product of the iap+ gene is diffusible.
  6. Taschner N, Yagil E, Spira B. A differential effect of sigmaS on the expression of the PHO regulon genes of Escherichia coli. Microbiology. 2004;150:2985-92 pubmed
    ..The interpretation of these observations is based on the competition between sigma(S) and sigma(D) for the binding to the core RNA polymerase. ..
  7. Bucević Popović V, Pavela Vrancic M, Dieckmann R. Metal-ion induced conformational changes in alkaline phosphatase from E. coli assessed by limited proteolysis. Biochimie. 2004;86:403-9 pubmed
    ..A specific shielding of a region distant from the metal-binding site has been demonstrated, implying transmission of conformational changes, induced by metal-ion binding to the adjacent subunit, across the subunit interface. ..
  8. Leyh T, Vogt T, Suo Y. The DNA sequence of the sulfate activation locus from Escherichia coli K-12. J Biol Chem. 1992;267:10405-10 pubmed
    ..During this work, another gene, iap, was partially sequenced and mapped. The activity of ATP sulfurylase is stimulated by an intrinsic GTPase...
  9. Nakata A, Amemura M, Makino K. Unusual nucleotide arrangement with repeated sequences in the Escherichia coli K-12 chromosome. J Bacteriol. 1989;171:3553-6 pubmed
    ..an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region...

More Information


  1. Nakata A, Shinagawa H, Amemura M. Cloning of alkaline phosphatase isozyme gene (iap) of Escherichia coli. Gene. 1982;19:313-9 pubmed
    ..A chromosomal gene, iap, is responsible for alkaline phosphatase isozyme conversion and is assumed to code for a proteolytic enzyme ..
  2. Nakata A, Shinagawa H, Shima H. Alkaline phosphatase isozyme conversion by cell-free extract of Escherichia coli. FEBS Lett. 1984;175:343-8 pubmed
    ..of type 1 isozyme with the supernatant of a sonicated cell-free extract prepared from the cells carrying the cloned iap+ gene on a multi-copy plasmid...
  3. Bachellier S, Clement J, Hofnung M. Short palindromic repetitive DNA elements in enterobacteria: a survey. Res Microbiol. 1999;150:627-39 pubmed
    ..Two repetitions, YPAL1 and YPAL2, found primarily in Yersinia, are described here for the first time. ..
  4. Golovastov V, Mikhaleva N, Nesmeyanova M. Depletion of phosphatidylethanolamine--the major membrane phospholipid of Escherichia coli--depresses posttranslocational modification of alkaline phosphatase in the periplasm. Biochemistry (Mosc). 2002;67:765-9 pubmed
    ..in phosphatidylethanolamine-depleted cells is supposed to be a result of the lower efficiency of secretion of modifying proteinase (product of the iap gene) localized, like alkaline phosphatase, in the periplasm of Escherichia coli.