Gene Symbol: hypD
Description: hydrogenase maturation protein
Alias: ECK2724, JW2699, hydF
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Rossmann R, Sawers G, Bock A. Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulon. Mol Microbiol. 1991;5:2807-14 pubmed
  2. Han K, Park J, Seo H, Ahn K, Lee J. Multiple stressor-induced proteome responses of Escherichia coli BL21(DE3). J Proteome Res. 2008;7:1891-903 pubmed publisher
    ..The quantitative and systematic proteome analyses that we have performed provide more detailed information on E. coli BL21(DE3), a widely used host strain for recombinant protein overexpression. ..
  3. Blokesch M, Bock A. Properties of the [NiFe]-hydrogenase maturation protein HypD. FEBS Lett. 2006;580:4065-8 pubmed
    A mutational screen of amino acid residues of hydrogenase maturation protein HypD from Escherichia coli disclosed that seven conserved cysteine residues located in three different motifs in HypD are essential...
  4. Blokesch M, Albracht S, Matzanke B, Drapal N, Jacobi A, Bock A. The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases. J Mol Biol. 2004;344:155-67 pubmed
    ..absence of carbamoylphosphate, cells accumulate a complex of two hydrogenase maturation proteins, namely HypC and HypD for the synthesis of hydrogenase 3...
  5. Maier T, Bock A. Generation of active [NiFe] hydrogenase in vitro from a nickel-free precursor form. Biochemistry. 1996;35:10089-93 pubmed
    ..The accessory proteins known to be involved in the maturation of HycE in vivo, namely HypB, HypC, HypD, HypE, HypF, and the protease HycI, are required for the in vitro reaction, since processing of HycE did not occur ..
  6. Stripp S, Soboh B, Lindenstrauss U, Braussemann M, Herzberg M, Nies D, et al. HypD is the scaffold protein for Fe-(CN)2CO cofactor assembly in [NiFe]-hydrogenase maturation. Biochemistry. 2013;52:3289-96 pubmed publisher
    NiFe]-hydrogenases bind a NiFe-(CN)2CO cofactor in their catalytic large subunit. The iron-sulfur protein HypD and the small accessory protein HypC play a central role in the generation of the CO and CN(-) ligands...
  7. Blokesch M, Paschos A, Bauer A, Reissmann S, Drapal N, Bock A. Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE. Eur J Biochem. 2004;271:3428-36 pubmed
    ..The results obtained with the HypE variants and also with mutant HypF forms are integrated to explain the complex reaction pattern of protein HypF. ..
  8. Blokesch M, Bock A. Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle. J Mol Biol. 2002;324:287-96 pubmed
    ..The DeltaCarAB mutant accumulated a complex consisting of the hydrogenase maturation proteins HypC and HypD. This complex was resolved upon citrulline addition and followed-up by the appearance of a complex between HypC and ..
  9. Sankar P, Shanmugam K. Hydrogen metabolism in Escherichia coli: biochemical and genetic evidence for a hydF gene. J Bacteriol. 1988;170:5446-51 pubmed
    ..This gene, termed hydF, mapped at 59 min in the E. coli chromosome and resided next to the hydB gene...

More Information


  1. Hopper S, Babst M, Schlensog V, Fischer H, Hennecke H, Bock A. Regulated expression in vitro of genes coding for formate hydrogenlyase components of Escherichia coli. J Biol Chem. 1994;269:19597-604 pubmed
    ..A supramolecular transcription complex is proposed which involves the binding of IHF to a site located between the UAS and the promoter responsible for transcription of the hyc operon. ..
  2. Butland G, Zhang J, Yang W, Sheung A, Wong P, Greenblatt J, et al. Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation. FEBS Lett. 2006;580:677-81 pubmed
    ..The complex also includes HypE and HypD, which interact with each other before joining the larger complex.
  3. Schlensog V, Bock A. Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli. Mol Microbiol. 1990;4:1319-27 pubmed
    ..FHLA appears to bind to the upstream regulatory sequence (URS) in the 5' flanking region of the fdhF gene since activation of fdhF expression was dependent on the presence of the URS. ..
  4. Forzi L, Sawers R. Maturation of [NiFe]-hydrogenases in Escherichia coli. Biometals. 2007;20:565-78 pubmed
    ..What is becoming increasingly clear is that the metabolic origins of the carbonyl group may be different. ..
  5. McGlynn S, Ruebush S, Naumov A, Nagy L, Dubini A, King P, et al. In vitro activation of [FeFe] hydrogenase: new insights into hydrogenase maturation. J Biol Inorg Chem. 2007;12:443-7 pubmed
    ..the heterologously expressed inactive HydA with extracts in which hydrogenase-specific maturation proteins HydE, HydF, and HydG are expressed in concert...
  6. Lutz S, Jacobi A, Schlensog V, Bohm R, Sawers G, Bock A. Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli. Mol Microbiol. 1991;5:123-35 pubmed
    ..Complementation analysis of previously characterized mutants showed that the hypB, hypC and hypD genes have a function in the formation of all three hydrogenase isoenzymes, lesions in hypB being complemented by ..
  7. Kuchenreuther J, Britt R, Swartz J. New insights into [FeFe] hydrogenase activation and maturase function. PLoS ONE. 2012;7:e45850 pubmed publisher
    ..The HydE, HydF, and HydG maturases assist in assembling the H-cluster and maturing hydrogenases into their catalytically active ..
  8. Jacobi A, Rossmann R, Bock A. The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli. Arch Microbiol. 1992;158:444-51 pubmed
    ..The products of three of the genes, hypB, hypD and hypE were found to be essential for the synthesis of all three hydrogenase isoenzymes...