Gene Symbol: hscB
Description: co-chaperone HscB
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Lelivelt M, Kawula T. Hsc66, an Hsp70 homolog in Escherichia coli, is induced by cold shock but not by heat shock. J Bacteriol. 1995;177:4900-7 pubmed
    ..Mapping of the transcription initiation site showed that hscA was cotranscribed with an upstream dnaJ-like gene, hscB; thus, hscB was also cold inducible...
  2. Vickery L, Silberg J, Ta D. Hsc66 and Hsc20, a new heat shock cognate molecular chaperone system from Escherichia coli. Protein Sci. 1997;6:1047-56 pubmed
    The hscA and hscB genes of Escherichia coli encode novel chaperone and co-chaperone proteins, designated Hsc66 and Hsc20, respectively. We have overproduced and purified Hsc66 and Hsc20 in high yield in E...
  3. Zheng L, Cash V, Flint D, Dean D. Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii. J Biol Chem. 1998;273:13264-72 pubmed
    ..vinelandii is that E. coli genes previously designated as hscB, hscA, and fdx are located immediately downstream from, and are probably co-transcribed with, the iscSUA genes...
  4. Tokumoto U, Takahashi Y. Genetic analysis of the isc operon in Escherichia coli involved in the biogenesis of cellular iron-sulfur proteins. J Biochem. 2001;130:63-71 pubmed
    ..Strains with mutations in the iscU, hscB, hscA, and fdx genes also exhibited conspicuous phenotypical consequences almost identical to one another...
  5. Cupp Vickery J, Vickery L. Crystal structure of Hsc20, a J-type Co-chaperone from Escherichia coli. J Mol Biol. 2000;304:835-45 pubmed
    ..Thus, Hsc20, in addition to its role in the regulation of the ATPase activity of Hsc66, may also function as a rigid scaffold to facilitate positioning of the protein substrates targeted to Hsc66. ..
  6. Hoff K, Ta D, Tapley T, Silberg J, Vickery L. Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU. J Biol Chem. 2002;277:27353-9 pubmed publisher
  7. Hoff K, Cupp Vickery J, Vickery L. Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system. J Biol Chem. 2003;278:37582-9 pubmed publisher
    Hsc66 (HscA) and Hsc20 (HscB) from Escherichia coli comprise a specialized chaperone system that selectively binds the iron-sulfur cluster template protein IscU...
  8. Silberg J, Hoff K, Vickery L. The Hsc66-Hsc20 chaperone system in Escherichia coli: chaperone activity and interactions with the DnaK-DnaJ-grpE system. J Bacteriol. 1998;180:6617-24 pubmed
    ..Together these results suggest that Hsc66-Hsc20 and DnaK-DnaJ-GrpE comprise separate molecular chaperone systems with distinct, nonoverlapping cellular functions. ..
  9. Tokumoto U, Nomura S, Minami Y, Mihara H, Kato S, Kurihara T, et al. Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. J Biochem. 2002;131:713-9 pubmed
    ..vitro binding and kinetic experiments, interactions were observed for the combinations of IscS and IscU, IscU and HscB, IscU and HscA, and HscB and HscA...

More Information


  1. Cupp Vickery J, Vickery L. Crystallization and preliminary X-ray crystallographic properties of Hsc20, a J-motif co-chaperone protein from Escherichia coli. Protein Sci. 1997;6:2028-30 pubmed
    ..6 A3/Da and a solvent content of approximately 53% in the crystals. Structure determination by isomorphous replacement is in progress. ..
  2. Nakamura M, Saeki K, Takahashi Y. Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hs cA-fdx-ORF3 gene cluster. J Biochem. 1999;126:10-8 pubmed
    ..We examined the coexpression of the E. coli ORF1-ORF2-iscS-iscU-iscA-hscB-hsc A-fdx-ORF3 gene cluster, which has recently been suggested to be involved in the formation or repair of Fe-S ..
  3. Campos García J, G Ordóñez L, Soberón Chávez G. The Pseudomonas aeruginosa hscA gene encodes Hsc66, a DnaK homologue. Microbiology. 2000;146 ( Pt 6):1429-35 pubmed
    ..characterized the transcriptional arrangement of hscA, showing that it forms part of an operon with the upstream hscB gene, and that it is also expressed from its own promoter. These results are compatible with the P...
  4. Sun G, Gargus J, Ta D, Vickery L. Identification of a novel candidate gene in the iron-sulfur pathway implicated in ataxia-susceptibility: human gene encoding HscB, a J-type co-chaperone. J Hum Genet. 2003;48:415-9 pubmed publisher
    ..most likely specifically involved in the same sub-process in iron-sulfur cluster assembly as one gene, designated hscB, in bacteria...
  5. Takahashi Y, Nakamura M. Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J Biochem. 1999;126:917-26 pubmed
    ..In Escherichia coli, genes in the ORF1-ORF2-iscS-iscU-iscA-hscB-hsc A-fdx-ORF3 cluster (the isc gene cluster) should be involved in the assembly of the Fe-S cluster since its ..
  6. Hoff K, Silberg J, Vickery L. Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli. Proc Natl Acad Sci U S A. 2000;97:7790-5 pubmed
    ..and purified the IscU protein from Escherichia coli and have studied its interactions with the hscA and hscB gene products Hsc66 and Hsc20...