Gene Symbol: hpt
Description: hypoxanthine phosphoribosyltransferase
Alias: ECK0124, JW5009
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hochstadt J. Hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase from enteric bacteria. Methods Enzymol. 1978;51:549-58 pubmed
  2. Page M, Burton K. The location of purine phosphoribosyltransferase activities in Escherichia coli. Biochem J. 1978;174:717-25 pubmed
  3. Jochimsen B, Nygaard P, Vestergaard T. Location on the chromosome of Escherichia coli of genes governing purine metabolism. Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt). Mol Gen Genet. 1975;143:85-91 pubmed
    ..The gene gsk encoding guanosine kinase was cotransducible with purE and shown to be located at 13 min. The gene hpt encoding hypoxanthine phosphoribosyltransferase was cotransducible with tonA indicating a location at 3 min...
  4. Holden J, Harriman P, Wall J. Escherichia coli mutants deficient in guanine-xanthine phosphoribosyltransferase. J Bacteriol. 1976;126:1141-8 pubmed
    ..Thus, the gpt gene is only one of at least two genes responsible for guanine PRTase activity in E. coli. ..
  5. Brikun I, Sukhodolets V. [Characteristics of the phenotypic manifestation of hpt and gpt mutations blocking 6-oxypurine utilization and their effect on the expression of catabolite-sensitive genes in the cells of an Escherichia coli K-12 purine auxotroph]. Genetika. 1985;21:902-13 pubmed
    ..of utilization of hypoxanthine, guanine and xanthine by means of phosphoribosyltransferases, as a result of the hpt and gpt mutations, have the Rel phenotype...
  6. Bruce I, Hardy J, Stacey K. Potentiation by purines of the growth-inhibitory effects of sulphonamides on Escherichia coli K12 and the location of the gene which mediates this effect. J Gen Microbiol. 1984;130:2489-95 pubmed
    ..This result suggests that these lysogens are heterozygous for psp and that the wild-type allele is dominant. It is probable that psp is a regulatory gene, affecting some rate-limiting step in the biosynthesis of methionine. ..
  7. Levine R, Taylor M. Selection for purine regulatory mutants in an E. coli hypoxanthine phosphoribosyl transferase-guanine phosphoribosyl transferase double mutant. Mol Gen Genet. 1981;181:313-8 pubmed
    ..led to the isolation of a hypoxanthine phosphoribosyl transferase-guanine phosphoribosyl transferase double mutant (hpt- gpt-) that is extremely sensitive to adenine...
  8. Brikun I, Sukhodolets V. [Phenotypic properties of Escherichia coli K-12 purine auxotrophs with disordered synthesis of guanine, xanthine and hypoxanthine phosphoribosyltransferases]. Genetika. 1981;17:932-5 pubmed
    ..blocked for reutilization of guanine, xanthine and hypoxanthine via phosphoribosiltransferase reactions (purD hpt gpt) were studied...
  9. Brikun I, Livshits V, Strauss M. [Genetic study of the mutations impairing guanine, xanthine and hypoxanthines assimilation in a purine auxotroph of Escherichia coli K-12]. Genetika. 1981;17:258-67 pubmed
    The hpt gene is responsible for the synthesis of hypoxanthine-guanine phosphoribosiltransferase...

More Information


  1. Izu H, Ito S, Elias M, Yamada M. Differential control by IHF and cAMP of two oppositely oriented genes, hpt and gcd, in Escherichia coli: significance of their partially overlapping regulatory elements. Mol Genet Genomics. 2002;266:865-72 pubmed
    The hpt gene, which encodes hypoxanthine phosphoribosyltransferase, is located next to, but transcribed in the opposite direction to, the gcd gene, which codes for a membrane-bound glucose dehydrogenase, at 3...
  2. Guddat L, Vos S, Martin J, Keough D, de Jersey J. Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase. Protein Sci. 2002;11:1626-38 pubmed
    ..However, hypoxanthine does not have a 2-exocyclic atom and the HPRT-IMP structure suggests that hypoxanthine is likely to occupy a different position in the purine-binding pocket. ..
  3. Scism R, Stec D, Bachmann B. Synthesis of nucleotide analogues by a promiscuous phosphoribosyltransferase. Org Lett. 2007;9:4179-82 pubmed
    ..This system offers an efficient and rapid method for nucleotide analogue synthesis with 100% beta-selectivity, providing analytically pure product in a single purification step. ..