hfq

Summary

Gene Symbol: hfq
Description: RNA-binding protein Hfq
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Le Derout J, Folichon M, Briani F, Deh G, R gnier P, Hajnsdorf E. Hfq affects the length and the frequency of short oligo(A) tails at the 3' end of Escherichia coli rpsO mRNAs. Nucleic Acids Res. 2003;31:4017-23 pubmed
    ..and distribution of poly(A) tails at the 3'-end of rpsO transcripts in vivo in the presence and absence of Hfq, a host factor that in vitro stimulates the activity of PAP I, and found that Hfq affects all three parameters...
  2. Hajnsdorf E, Regnier P. Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I. Proc Natl Acad Sci U S A. 2000;97:1501-5 pubmed
    ..Here, we show that host factor Hfq is also involved in poly(A) tail metabolism...
  3. Wachi M, Takada A, Nagai K. Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant. Biochem Biophys Res Commun. 1999;264:525-9 pubmed
    We found that 82- and 76-kDa proteins in the outer-membrane fractions were overproduced in the hfq::cat mutant cells when grown in synthetic media...
  4. Moll I, Leitsch D, Steinhauser T, Bl si U. RNA chaperone activity of the Sm-like Hfq protein. EMBO Rep. 2003;4:284-9 pubmed publisher
    The Escherichia coli Sm-like host factor I (Hfq) protein is thought to function in post-transcriptional regulation by modulating the function of small regulatory RNAs. Hfq also interferes with ribosome binding on E...
  5. Muffler A, Fischer D, Hengge Aronis R. The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli. Genes Dev. 1996;10:1143-51 pubmed
    ..Here, we identify the hfq-encoded RNA-binding protein HF-I, which has been known previously only as a host factor for the replication of ..
  6. Su Q, Schuppli D, Tsui HcT -, Winkler M, Weber H. Strongly reduced phage Qbeta replication, but normal phage MS2 replication in an Escherichia coli K12 mutant with inactivated Qbeta host factor (hfq) gene. Virology. 1997;227:211-4 pubmed
    RNA phage production was observed in Escherichia coli hfq mutant strains transformed with plasmids containing genomic cDNA of phages Qbeta or MS2...
  7. Vytvytska O, Jakobsen J, Balcunaite G, Andersen J, Baccarini M, von Gabain A. Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability. Proc Natl Acad Sci U S A. 1998;95:14118-23 pubmed
    ..An RNA-binding protein with affinity for the 5' untranslated region ompA was purified and identified as Hfq, a host factor initially recognized for its function in phage Qbeta replication...
  8. Nakao H, Watanabe H, Nakayama S, Takeda T. yst gene expression in Yersinia enterocolitica is positively regulated by a chromosomal region that is highly homologous to Escherichia coli host factor 1 gene (hfq). Mol Microbiol. 1995;18:859-65 pubmed
    ..The yrp gene mutation caused decreased negative supercoiling of plasmids, as did hfq. The yrp gene could similarly complement Y-ST production in two other silent strains of Y. enterocolitica...
  9. Muffler A, Traulsen D, Fischer D, Lange R, Hengge Aronis R. The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the sigmaS subunit of RNA polymerase in Escherichia coli. J Bacteriol. 1997;179:297-300 pubmed
    The hfq-encoded RNA-binding protein HF-I has long been known as a host factor for phage Qbeta RNA replication and has recently been shown to be essential for translation of rpoS, which encodes the sigmaS subunit of RNA polymerase...

More Information

Publications63

  1. Roop R, Gee J, Robertson G, Richardson J, Ng W, Winkler M. Brucella stationary-phase gene expression and virulence. Annu Rev Microbiol. 2003;57:57-76 pubmed publisher
    ..The RNA binding protein host factor I (HF-I) encoded by the hfq gene is required for the efficient translation of the stationary-phase sigma factor RpoS in many bacteria, and a ..
  2. Hengge Aronis R. Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli. Mol Microbiol. 1996;21:887-93 pubmed
    ..Taken together, sigma S function is clearly not confined to stationary phase. Rather, sigma S may be regarded as a sigma factor associated with general stress conditions. ..
  3. Schuppli D, Georgijevic J, Weber H. Synergism of mutations in bacteriophage Qbeta RNA affecting host factor dependence of Qbeta replicase. J Mol Biol. 2000;295:149-54 pubmed
    We have recently shown that Escherichia coli cells deficient in Hfq protein (i.e...
  4. Schulz E, Barabas O. Structure of an Escherichia coli Hfq:RNA complex at 0.97 Å resolution. Acta Crystallogr F Struct Biol Commun. 2014;70:1492-7 pubmed publisher
    ..A central player in this process is the RNA chaperone Hfq, which facilitates the annealing of sRNAs with their target mRNAs...
  5. Mass E, Majdalani N, Gottesman S. Regulatory roles for small RNAs in bacteria. Curr Opin Microbiol. 2003;6:120-4 pubmed
    ..These include many that require the RNA-binding protein Hfq for their activity; most of these RNAs act by pairing with their target mRNAs...
  6. Ali Azam T, Iwata A, Nishimura A, Ueda S, Ishihama A. Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid. J Bacteriol. 1999;181:6361-70 pubmed
    ..DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid ..
  7. Tsui H, Feng G, Winkler M. Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12. J Bacteriol. 1997;179:7476-87 pubmed
    ..we tested whether the amounts of MutS, MutL, and MutH were regulated by two global regulators, RpoS (sigma38) and Hfq (HF-I [putative RNA chaperone]), which are involved in stationary-phase transition...
  8. Kajitani M, Ishihama A. Identification and sequence determination of the host factor gene for bacteriophage Q beta. Nucleic Acids Res. 1991;19:1063-6 pubmed
    ..The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and ..
  9. Hirsch M, Elliott T. Role of ppGpp in rpoS stationary-phase regulation in Escherichia coli. J Bacteriol. 2002;184:5077-87 pubmed
    ..of other genes that have been suggested as regulators of rpoS were tested, including dksA, dsrA, barA, ppkx, and hfq. With the exception of the dksA mutant, which had a modest defect in Luria-Bertani medium, none of these mutants ..
  10. Sonnleitner E, Hagens S, Rosenau F, Wilhelm S, Habel A, J ger K, et al. Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1. Microb Pathog. 2003;35:217-28 pubmed
    The Sm-like protein Hfq has been implicated in the regulation of sigmaS-dependent and sigmaS-independent genes in E. coli and in the regulation of virulence factors in both, Yersinia enterocolitica and Brucella abortus...
  11. Shi X, Bennett G. Plasmids bearing hfq and the hns-like gene stpA complement hns mutants in modulating arginine decarboxylase gene expression in Escherichia coli. J Bacteriol. 1994;176:6769-75 pubmed
    ..Another group was found to carry the hfq gene, which encodes the host factor HF-1 for bacteriophage Q beta replication...
  12. Zhang A, Wassarman K, Ortega J, Steven A, Storz G. The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs. Mol Cell. 2002;9:11-22 pubmed
    The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhlA and rpoS mRNA translation...
  13. Kajitani M, Kato A, Wada A, Inokuchi Y, Ishihama A. Regulation of the Escherichia coli hfq gene encoding the host factor for phage Q beta. J Bacteriol. 1994;176:531-4 pubmed
    The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome...
  14. Vasil eva I, Garber M. [The regulatory role of the Hfq protein in bacterial cells]. Mol Biol (Mosk). 2002;36:970-7 pubmed
    b>Hfq (HF1) is a polyfunctional translational regulator of numerous bacterial mRNAs...
  15. Rajkumari K, Gowrishankar J. In vivo expression from the RpoS-dependent P1 promoter of the osmotically regulated proU operon in Escherichia coli and Salmonella enterica serovar Typhimurium: activation by rho and hns mutations and by cold stress. J Bacteriol. 2001;183:6543-50 pubmed publisher
    ..In cells lacking the RNA-binding regulator protein Hfq, induction of E...
  16. Ishihama A. Modulation of the nucleoid, the transcription apparatus, and the translation machinery in bacteria for stationary phase survival. Genes Cells. 1999;4:135-43 pubmed
    ..The major DNA-binding proteins, Fis, HU and Hfq, in the exponential-phase nucleoid are replaced by a single stationary-phase protein Dps, thereby compacting the ..
  17. Brown L, Elliott T. Mutations that increase expression of the rpoS gene and decrease its dependence on hfq function in Salmonella typhimurium. J Bacteriol. 1997;179:656-62 pubmed
    ..Previous work with rpoS-lac protein fusions has suggested that translation of rpoS requires hfq function...
  18. Brown L, Elliott T. Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene. J Bacteriol. 1996;178:3763-70 pubmed
    ..Here we report that host factor I (HF-I), the product of the hfq gene, is required for efficient expression of rpoS in S. typhimurium...
  19. Vassilieva I, Nikulin A, Blasi U, Moll I, Garber M. Crystallization of Hfq protein: a bacterial gene-expression regulator. Acta Crystallogr D Biol Crystallogr. 2003;59:1061-3 pubmed
    b>Hfq protein from Escherichia coli (EcoHfq) has been overproduced in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique...
  20. Ruiz N, Silhavy T. Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion. J Bacteriol. 2003;185:5984-92 pubmed
    ..This upregulation of rpoS translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA)...
  21. Tsui H, Feng G, Winkler M. Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered Esigma32-specific promoters during heat shock. J Bacteriol. 1996;178:5719-31 pubmed
    The amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of Escherichia coli contains genes that are important for diverse cellular functions, including DNA mismatch repair (mutL), tRNA modification (miaA), pleiotropic regulation (hfq), and ..
  22. Lease R, Belfort M. Riboregulation by DsrA RNA: trans-actions for global economy. Mol Microbiol. 2000;38:667-72 pubmed
    ..Additionally, the RNA chaperone protein Hfq is involved with DsrA regulation, as well as with other small RNAs that also act on RpoS to co-ordinate stress ..
  23. Loewen P, Hu B, Strutinsky J, Sparling R. Regulation in the rpoS regulon of Escherichia coli. Can J Microbiol. 1998;44:707-17 pubmed
    ..Translation of rpoS mRNA is controlled by a cascade of interacting factors, including Hfq, H-NS, dsrA RNA, LeuO, and oxyS RNA that seem to modulate the stability of a region of secondary structure in the ..
  24. Schuppli D, Miranda G, Tsui H, Winkler M, Sogo J, Weber H. Altered 3'-terminal RNA structure in phage Qbeta adapted to host factor-less Escherichia coli. Proc Natl Acad Sci U S A. 1997;94:10239-42 pubmed
    The RNA phage Qbeta requires for the replication of its genome an RNA binding protein called Qbeta host factor or Hfq protein...
  25. M ller T, Franch T, H jrup P, Keene D, B chinger H, Brennan R, et al. Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction. Mol Cell. 2002;9:23-30 pubmed
    The bacterial Hfq protein modulates the stability or the translation of mRNAs and has recently been shown to interact with small regulatory RNAs in E. coli...
  26. Mass E, Escorcia F, Gottesman S. Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli. Genes Dev. 2003;17:2374-83 pubmed publisher
    ..RyhB requires the RNA chaperone Hfq. In the absence of Hfq, RyhB is unstable, even when general transcription is inhibited; degradation is dependent ..
  27. Sukhodolets M, Garges S. Interaction of Escherichia coli RNA polymerase with the ribosomal protein S1 and the Sm-like ATPase Hfq. Biochemistry. 2003;42:8022-34 pubmed publisher
    We report evidence that ribosomal protein S1 and nucleic acid-binding protein Hfq copurify in molar ratios with RNA polymerase (RNAP)...
  28. Sun X, Zhulin I, Wartell R. Predicted structure and phyletic distribution of the RNA-binding protein Hfq. Nucleic Acids Res. 2002;30:3662-71 pubmed
    b>Hfq, a bacterial RNA-binding protein, was recently shown to contain the Sm1 motif, a characteristic of Sm and LSm proteins that function in RNA processing events in archaea and eukaryotes...
  29. Ueno H, Yonesaki T. Role of Escherichia coli Hfq in late-gene silencing of bacteriophage T4 dmd mutant. Genes Genet Syst. 2002;77:301-8 pubmed
    ..Here we show that the host hfq gene is involved in the rapid mRNA degradation in a dmd mutant...
  30. Zhang A, Altuvia S, Tiwari A, Argaman L, Hengge Aronis R, Storz G. The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein. EMBO J. 1998;17:6061-8 pubmed
    ..This repression is dependent on the hfq-encoded RNA-binding protein (also denoted host factor I, HF-I)...
  31. Vytvytska O, Moll I, Kaberdin V, von Gabain A, Blasi U. Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. Genes Dev. 2000;14:1109-18 pubmed
    ..Recently, host factor I (Hfq/HF1) was shown to be involved in the regulation of ompA mRNA stability under slow growth conditions...
  32. Arluison V, Derreumaux P, Allemand F, Folichon M, Hajnsdorf E, R gnier P. Structural Modelling of the Sm-like Protein Hfq from Escherichia coli. J Mol Biol. 2002;320:705-12 pubmed
    The Hfq polypeptide of Escherichia coli is a nucleic acid-binding protein involved in the expression of many proteins...
  33. Donahue W, Jarrell K. A BLAST from the past: ancient origin of human Sm proteins. Mol Cell. 2002;9:7-8 pubmed
    In this issue of Molecular Cell, Zhang et al. and Møller et al. independently report studies of the E. coli Hfq protein, revealing significant sequence similarities with human Sm proteins...
  34. Azam T, Ishihama A. Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999;274:33105-13 pubmed
    ..DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid ..
  35. Moll I, Afonyushkin T, Vytvytska O, Kaberdin V, Bl si U. Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA. 2003;9:1308-14 pubmed
    The Escherichia coli RNA chaperone Hfq was discovered originally as an accessory factor of the phage Qbeta replicase...
  36. Robertson G, Roop R. The Brucella abortus host factor I (HF-I) protein contributes to stress resistance during stationary phase and is a major determinant of virulence in mice. Mol Microbiol. 1999;34:690-700 pubmed
    ..In order to evaluate this relationship, we examined the in vitro and in vivo characteristics of an isogenic hfq mutant constructed from virulent Brucella abortus 2308...
  37. Hengge Aronis R. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase. Microbiol Mol Biol Rev. 2002;66:373-95, table of contents pubmed
    ..rpoS translation is controlled by several proteins (Hfq and HU) and small regulatory RNAs that probably affect the secondary structure of rpoS mRNA...
  38. Kaminski P, Desnoues N, Elmerich C. The expression of nifA in Azorhizobium caulinodans requires a gene product homologous to Escherichia coli HF-I, an RNA-binding protein involved in the replication of phage Q beta RNA. Proc Natl Acad Sci U S A. 1994;91:4663-7 pubmed
    ..The similarity can be extended to the function since hfq, the structural gene for HF-I, complemented the A. caulinodans mutant. The corresponding gene in A...
  39. Tsui H, Leung H, Winkler M. Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12. Mol Microbiol. 1994;13:35-49 pubmed
    The region immediately downstream from the miaA tRNA modification gene at 94.8 min contains the hfq gene and the hflA region, which are important in the bacteriophage Q beta and lambda life cycles...
  40. Sonnleitner E, Moll I, Bl si U. Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa. Microbiology. 2002;148:883-91 pubmed publisher
    The 102 aa Hfq protein of Escherichia coli (Hfq(Ec)) was first described as a host factor required for phage Qbeta replication. More recently, Hfq was shown to affect the stability of several E...
  41. Mass E, Gottesman S. A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli. Proc Natl Acad Sci U S A. 2002;99:4620-5 pubmed publisher
    ..RyhB requires the RNA-binding protein, Hfq, for activity...
  42. Tsui H, Winkler M. Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation. Biochimie. 1994;76:1168-77 pubmed
    The complex amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of E coli contains important genes for several fundamental cellular processes, including cell-wall hydrolysis (amiB), DNA repair (mutL), tRNA modification (miaA) and proteolysis (..
  43. Takada A, Wachi M, Kaidow A, Takamura M, Nagai K. DNA binding properties of the hfq gene product of Escherichia coli. Biochem Biophys Res Commun. 1997;236:576-9 pubmed
    ..N-terminal amino acid residues of the 15-kDa protein was identical with the host factor I (HF-I) encoded by the hfq gene at 95 min on the Escherichia coli chromosome map, which is known to be required for bacteriophage Qbeta ..
  44. Drepper T, Raabe K, Giaourakis D, Gendrullis M, Masepohl B, Klipp W. The Hfq-like protein NrfA of the phototrophic purple bacterium Rhodobacter capsulatus controls nitrogen fixation via regulation of nifA and anfA expression. FEMS Microbiol Lett. 2002;215:221-7 pubmed
    ..the nif (nitrogen fixation) regulatory factor NrfA of Azorhizobium caulinodans and the nucleoid-associated protein Hfq of Escherichia coli. Mutational analysis revealed that, in contrast to the situation in A...
  45. Nogueira T, Springer M. Post-transcriptional control by global regulators of gene expression in bacteria. Curr Opin Microbiol. 2000;3:154-8 pubmed
    ..This is the case for Hfq (HF-1), which is involved in the regulation of an increasing number of genes in Escherichia coli, and CsrA (RsmA) ..
  46. Brescia C, Mikulecky P, Feig A, Sledjeski D. Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure. RNA. 2003;9:33-43 pubmed
    ..DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo...
  47. Cunning C, Brown L, Elliott T. Promoter substitution and deletion analysis of upstream region required for rpoS translational regulation. J Bacteriol. 1998;180:4564-70 pubmed
    ..Regulation of these fusions required the function of hfq, encoding the RNA-binding protein host factor I (HF-I)...
  48. Takada A, Wachi M, Nagai K. Negative regulatory role of the Escherichia coli hfq gene in cell division. Biochem Biophys Res Commun. 1999;266:579-83 pubmed
    We found that the hfq::cat mutant strain produced minicells at high frequency...
  49. Kaminski P, Elmerich C. The control of Azorhizobium caulinodans nifA expression by oxygen, ammonia and by the HF-I-like protein, NrfA. Mol Microbiol. 1998;28:603-13 pubmed
    ..NrfA also restores the defect in rpoS translation to an Escherichia coli hfq mutant, indicating that HF-I and NrfA have similar activities in both A. caulinodans and E. coli.
  50. Vassilieva I, Rouzanov M, Zelinskaya N, Moll I, Bl si U, Garber M. Cloning, purification, and crystallization of a bacterial gene expression regulator--Hfq protein from Escherichia coli. Biochemistry (Mosc). 2002;67:1293-7 pubmed
    Thermostable RNA-binding protein Hfq (also denoted HF1) is a multifunctional expression regulator of many bacterial genes...
  51. Azam T, Hiraga S, Ishihama A. Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells. 2000;5:613-26 pubmed
    ..Here we show that the majority of Hfq is present in cytoplasm together with ribosomal proteins L7/L12 and RMF. The DNA-binding proteins of E...
  52. Klauck E, Böhringer J, Hengge Aronis R. The LysR-like regulator LeuO in Escherichia coli is involved in the translational regulation of rpoS by affecting the expression of the small regulatory DsrA-RNA. Mol Microbiol. 1997;25:559-69 pubmed
    ..Regulatory factors involved in this control are the RNA-binding Hfq (HF-I) protein, the histone-like protein H-NS and the small regulatory DsrA-RNA (with the last being specifically ..
  53. Sledjeski D, Whitman C, Zhang A. Hfq is necessary for regulation by the untranslated RNA DsrA. J Bacteriol. 2001;183:1997-2005 pubmed
    ..b>Hfq is an E. coli protein necessary for the in vitro and in vivo replication of the RNA phage Qbeta...
  54. Sauter C, Basquin J, Suck D. Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli. Nucleic Acids Res. 2003;31:4091-8 pubmed
    The Hfq protein was discovered in Escherichia coli in the early seventies as a host factor for the Qbeta phage RNA replication...