Gene Symbol: hdhA
Description: 7-alpha-hydroxysteroid dehydrogenase, NAD-dependent
Alias: ECK1614, JW1611, hsd, hsdH
Species: Escherichia coli str. K-12 substr. MG1655
Yoshimoto T, Tone H, Honda T, Osatomi K, Kobayashi R, Tsuru D. Sequencing and high expression of aminopeptidase P gene from Escherichia coli HB101. J Biochem. 1989;105:412-6 pubmed
..The molecular weight of the enzyme was estimated to be 49,650 from the nucleotide sequence. The purified enzyme contained 0.2 gram atom of zinc per subunit. The enzyme activity was inhibited by EDTA and activated 5-fold by Mn2+. ..
Tanaka N, Nonaka T, Tanabe T, Yoshimoto T, Tsuru D, Mitsui Y. Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli. Biochemistry. 1996;35:7715-30 pubmed
7 alpha-Hydroxysteroid dehydrogenase (7 alpha-HSDH;1 EC 184.108.40.206) is an NAD+-dependent oxidoreductase belonging to the short-chain dehydrogenase/reductase (SDR) 1 family...
Weber H, Polen T, Heuveling J, Wendisch V, Hengge R. Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity. J Bacteriol. 2005;187:1591-603 pubmed
..Thus, not only the expression of genes within a regulatory network but also the architecture of the network itself can be subject to regulation. ..
Yoshimoto T, Higashi H, Kanatani A, Lin X, Nagai H, Oyama H, et al
. Cloning and sequencing of the 7 alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme. J Bacteriol. 1991;173:2173-9 pubmed
..The enzyme was NAD+ dependent and had a pI of 4.3. The molecular mass was estimated to be 120 kDa by the gel filtration method and 28 kDa by electrophoresis, indicating that the enzyme exists in a tetrameric form. ..
Yoshimoto T, Nagai H, Ito K, Tsuru D. Location of the 7 alpha-hydroxysteroid dehydrogenase gene (hdhA) on the physical map of the Escherichia coli chromosome. J Bacteriol. 1993;175:5730 pubmed
Tanabe T, Tanaka N, Uchikawa K, Kabashima T, Ito K, Nonaka T, et al
. Roles of the Ser146, Tyr159, and Lys163 residues in the catalytic action of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli. J Biochem. 1998;124:634-41 pubmed
The Escherichia coli 7alpha-hydroxysteroid dehydrogenase (7alpha-HSDH; EC 1.1.1...
Weber A, Kögl S, Jung K. Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli. J Bacteriol. 2006;188:7165-75 pubmed
..The protein profiles revealed an induction of 12 proteins (Dps, HchA, HdhA, InfB, OsmC, OsmY, ProX, KatE, PspA, TalA, TktB, and TreF) under osmotic stress in an aerobic milieu...