Gene Symbol: gnd
Description: 6-phosphogluconate dehydrogenase, decarboxylating
Alias: ECK2024, JW2011
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Peyru G, Fraenkel D. Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli. J Bacteriol. 1968;95:1272-8 pubmed
    ..affecting the constitutive enzymes glucose-6-phosphate dehydrogenase (zwf) and gluconate-6-phosphate dehydrogenase (gnd), and the inducible enzyme gluconate-6-phosphate dehydrase (edd), were determined by conjugation and transduction ..
  2. Pease A, Wolf R. Determination of the growth rate-regulated steps in expression of the Escherichia coli K-12 gnd gene. J Bacteriol. 1994;176:115-22 pubmed
    ..Previous work with gnd-lac operon and protein fusion strains indicated that two steps in the expression of the gnd gene are subject to ..
  3. Nasoff M, Wolf R. Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli. J Bacteriol. 1980;143:731-41 pubmed
    Expression of the gene gnd of Escherichia coli, which encodes 6-phosphogluconate dehydrogenase, is regulated by growth rate...
  4. Neuhard J, Thomassen E. Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene. J Bacteriol. 1976;126:999-1001 pubmed
    ..The lack of uridine kinase makes a positive selection possible for these strains. Due to the dcd mutation, P2 eductants show large alterations in their deoxyribonucleoside triphosphate pools. ..
  5. Thomson J, Gerstenberger P, Goldberg D, Gociar E, Orozco de Silva A, Fraenkel D. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J Bacteriol. 1979;137:502-6 pubmed
    ..phosphoglucose isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf), gluconate-6-phosphate dehydrogenase (gnd), enolase (eno), phosphoglycerate kinase (pgk), and fructose-1,6-P2 aldolase (fda)...
  6. Veronese F, Boccu E, Fontana A. Isolation and properties of 6-phosphogluconate dehydrogenase from Escherichia coli. Some comparisons with the thermophilic enzyme from Bacillus stearothermophilus. Biochemistry. 1976;15:4026-33 pubmed
    ..The results show that the mesophilic and thermophilic enzymes are very similar and that differences in thermal stability depend on subtle differences in the architectures of the proteins. ..
  7. Miller R, Dykhuizen D, Hartl D. Fitness effects of a deletion mutation increasing transcription of the 6-phosphogluconate dehydrogenase gene in Escherichia coli. Mol Biol Evol. 1988;5:691-703 pubmed
    ..The selectively favored mutation, designated gnd+ (862), occurred in the gene gnd coding for 6-phosphogluconate dehydrogenase, used in gluconate metabolism...
  8. Barcak G, Wolf R. Comparative nucleotide sequence analysis of growth-rate-regulated gnd alleles from natural isolates of Escherichia coli and from Salmonella typhimurium LT-2. J Bacteriol. 1988;170:372-9 pubmed
    A comparative study of gnd genes from Escherichia coli strains isolated from natural populations and laboratory strains and from Salmonella typhimurium was undertaken. In the accompanying paper (G. J. Barcak and R. E. Wolf, Jr., J...
  9. Chen Y, Ko T, Chen W, Lo L, Lin C, Wang A. Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism. J Struct Biol. 2010;169:25-35 pubmed publisher
    ..We propose that the catalysis follows an ordered binding mechanism with alternating conformational changes in the corresponding subunits, involving several related amino acid residues. ..

More Information


  1. Wolf R, Cool J. Mapping of insertion mutations in gnd of Escherichia coli with deletions defining the ends of the gene. J Bacteriol. 1980;141:1222-9 pubmed
    A genetic map was prepared for gnd, the gene of Escherichia coli which encodes the metabolically regulated 6-phosphogluconate dehydrogenase...
  2. Siddiquee K, Arauzo Bravo M, Shimizu K. Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli. FEMS Microbiol Lett. 2004;235:25-33 pubmed
    ..and mdh genes were strongly up-regulated, and that the oxidative pentose phosphate pathway genes such as zwf and gnd were significantly up-regulated in the pykF mutant...
  3. Wolf R, Shea F. Combined use of strain construction and affinity chromatography in the rapid, high-yield purification of 6-phosphogluconate dehydrogenase from Escherichia coli. J Bacteriol. 1979;138:171-5 pubmed
    ..Fructose 1,6-diphosphate is an inhibitor of enzyme activity. ..
  4. Nasoff M, Baker H, Wolf R. DNA sequence of the Escherichia coli gene, gnd, for 6-phosphogluconate dehydrogenase. Gene. 1984;27:253-64 pubmed
    Expression of gnd of Escherichia coli, which encodes 6-phosphogluconate dehydrogenase, an enzyme of the hexose monophosphate shunt, is subject to growth rate-dependent regulation and is gene dosage-dependent: the level of the enzyme ..
  5. Chang J, Green C, Wolf R. Inhibition of translation initiation on Escherichia coli gnd mRNA by formation of a long-range secondary structure involving the ribosome binding site and the internal complementary sequence. J Bacteriol. 1995;177:6560-7 pubmed
    Previous research has indicated that the growth rate-dependent regulation of Escherichia coli gnd expression involves the internal complementary sequence (ICS), a negative control site that lies within the 6-phosphogluconate ..
  6. Zhao J, Baba T, Mori H, Shimizu K. Global metabolic response of Escherichia coli to gnd or zwf gene-knockout, based on 13C-labeling experiments and the measurement of enzyme activities. Appl Microbiol Biotechnol. 2004;64:91-8 pubmed
    ..glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were constructed by disrupting the zwf and gnd genes and were grown in minimal media with two different carbon sources, such as glucose or pyruvate...
  7. Fraenkel D. Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J Bacteriol. 1968;95:1267-71 pubmed
    ..Thus, these enzymes are not essential for glucose metabolism in E. coli. ..
  8. Sly L, Doelle H. 6-phosphogluconate dehydrogenase in cell free extracts of Escherichia coli K-12. Arch Mikrobiol. 1968;63:214-23 pubmed
  9. Sandoval J, Arenas F, García J, Díaz Vásquez W, Valdivia González M, Sabotier M, et al. Escherichia coli 6-phosphogluconate dehydrogenase aids in tellurite resistance by reducing the toxicant in a NADPH-dependent manner. Microbiol Res. 2015;177:22-7 pubmed publisher
    ..The enzyme responsible for toxicant reduction was identified as 6-phosphogluconate dehydrogenase (Gnd)...
  10. Sunshine M, Kelly B. Extent of host deletions associated with bacteriophage P2-mediated eduction. J Bacteriol. 1971;108:695-704 pubmed
    ..H, the his operon, a suppressor of the recBC phenotype (sbcB), the gene for gluconate-6-phosphate dehydrogenase (gnd), a locus involved in cell wall synthesis (rfb), and in some cases all or part of genes involved in ..
  11. Jiao Z, Baba T, Mori H, Shimizu K. Analysis of metabolic and physiological responses to gnd knockout in Escherichia coli by using C-13 tracer experiment and enzyme activity measurement. FEMS Microbiol Lett. 2003;220:295-301 pubmed
    The physiological and metabolic responses to gnd knockout in Escherichia coli K-12 was quantitatively investigated by using the (13)C tracer experiment (GC-MS/NMR) together with the enzyme activity analysis...
  12. Lee J, Shin H, Lee Y. Metabolic engineering of pentose phosphate pathway in Ralstoniaeutropha for enhanced biosynthesis of poly-beta-hydroxybutyrate. Biotechnol Prog. 2003;19:1444-9 pubmed
    ..The gnd gene encoding 6-phosphogluconate dehydrogenase (6PGDH) and the tktA gene encoding the transketolase (TK) in PP ..