Gene Symbol: gloB
Description: hydroxyacylglutathione hydrolase
Alias: ECK0212, JW0202, yafR
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Thornalley P. The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life. Biochem J. 1990;269:1-11 pubmed
  2. Murata K, Tani K, Kato J, Chibata I. Excretion of glutathione by methylglyoxal-resistant Escherichia coli. J Gen Microbiol. 1980;120:545-7 pubmed
    ..Methylglyoxal resistance appeared to be due to the simultaneous increase in the activities of these two enzyme systems. ..
  3. Cooper R, Anderson A. The formation and catabolism of methylglyoxal during glycolysis in Escherichia coli. FEBS Lett. 1970;11:273-276 pubmed
  4. Misra K, Banerjee A, Ray S, Ray M. Glyoxalase III from Escherichia coli: a single novel enzyme for the conversion of methylglyoxal into D-lactate without reduced glutathione. Biochem J. 1995;305 ( Pt 3):999-1003 pubmed
  5. Ferguson G, Tötemeyer S, Maclean M, Booth I. Methylglyoxal production in bacteria: suicide or survival?. Arch Microbiol. 1998;170:209-18 pubmed
    ..New strategies for antibacterial therapy may be based on undermining the detoxification and defence mechanisms coupled with deregulation of methylglyoxal synthesis. ..
  6. Campos Bermudez V, Leite N, Krog R, Costa Filho A, Soncini F, Oliva G, et al. Biochemical and structural characterization of Salmonella typhimurium glyoxalase II: new insights into metal ion selectivity. Biochemistry. 2007;46:11069-79 pubmed publisher
    ..from Salmonella typhimurium that we demonstrate, on the basis of its activity, to be a glyoxalase II, named GloB. Recombinant GloB expressed in Escherichia coli was purified with variable amounts of iron, zinc, and manganese...
  7. Booth I, Ferguson G, Miller S, Li C, Gunasekera B, Kinghorn S. Bacterial production of methylglyoxal: a survival strategy or death by misadventure?. Biochem Soc Trans. 2003;31:1406-8 pubmed
    ..Excessive production of MG is an adaptive ploy, which, if it fails, has fatal consequences. On this basis one might define MG-induced loss of life as "death by misadventure" rather than suicide! ..
  8. O Young J, Sukdeo N, Honek J. Escherichia coli glyoxalase II is a binuclear zinc-dependent metalloenzyme. Arch Biochem Biophys. 2007;459:20-6 pubmed
    ..Unlike E. coli GlxI, which is non-Zn2+-dependent, Zn2+ activates the E. coli GlxII enzyme, with no evidence for Ni2+ metal utilization. ..
  9. Cooper R. Metabolism of methylglyoxal in microorganisms. Annu Rev Microbiol. 1984;38:49-68 pubmed

More Information


  1. Okado Matsumoto A, Fridovich I. The role of alpha,beta -dicarbonyl compounds in the toxicity of short chain sugars. J Biol Chem. 2000;275:34853-7 pubmed
    ..Moreover, the defensive glyoxalase III is also inactivated by the oxidative stress imposed by the lack of SOD, thereby exacerbating the deleterious effect of sugar oxidation. ..