glnL

Summary

Gene Symbol: glnL
Description: sensory histidine kinase in two-component regulatory system with GlnG
Alias: ECK3862, JW3840, glnR, ntrB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Jiang P, Ninfa A. Regulation of autophosphorylation of Escherichia coli nitrogen regulator II by the PII signal transduction protein. J Bacteriol. 1999;181:1906-11 pubmed
    The nitrogen regulator II (NRII or NtrB)-NRI (NtrC) two-component signal transduction system regulates the transcription of nitrogen-regulated genes in Escherichia coli...
  2. Pioszak A, Ninfa A. Genetic and biochemical analysis of phosphatase activity of Escherichia coli NRII (NtrB) and its regulation by the PII signal transduction protein. J Bacteriol. 2003;185:1299-315 pubmed
    Mutant forms of Escherichia coli NRII (NtrB) were isolated that retained wild-type NRII kinase activity but were defective in the PII-activated phosphatase activity of NRII...
  3. Ninfa A, Bennett R. Identification of the site of autophosphorylation of the bacterial protein kinase/phosphatase NRII. J Biol Chem. 1991;266:6888-93 pubmed
    ..have established that the Escherichia coli protein kinase/phosphatase nitrogen regulator II (NRII also known as NtrB) becomes autophosphorylated on a histidine residue when incubated with ATP...
  4. Ninfa A, Magasanik B. Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli. Proc Natl Acad Sci U S A. 1986;83:5909-13 pubmed
    ..requires the glnG gene product, NRI, as well as the rpoN(glnF) gene product, sigma60, and is regulated by the glnL gene product, NRII...
  5. Jiang P, Peliska J, Ninfa A. Reconstitution of the signal-transduction bicyclic cascade responsible for the regulation of Ntr gene transcription in Escherichia coli. Biochemistry. 1998;37:12795-801 pubmed
    ..enzyme (UTase/UR), its substrate the signal transduction protein PII, and the kinase/phosphatase NRII (NtrB), which is a PII receptor that phosphorylates and dephosphorylates NRI...
  6. Keener J, Kustu S. Protein kinase and phosphoprotein phosphatase activities of nitrogen regulatory proteins NTRB and NTRC of enteric bacteria: roles of the conserved amino-terminal domain of NTRC. Proc Natl Acad Sci U S A. 1988;85:4976-80 pubmed
    ..Both phosphorylation and dephosphorylation of NTRC occur in the presence of the NTRB protein (ntrB product)...
  7. Pioszak A, Jiang P, Ninfa A. The Escherichia coli PII signal transduction protein regulates the activities of the two-component system transmitter protein NRII by direct interaction with the kinase domain of the transmitter module. Biochemistry. 2000;39:13450-61 pubmed
    ..These results indicate that PII controls NRII by interaction with the conserved kinase domain of the transmitter module. ..
  8. Pioszak A, Ninfa A. Mechanism of the PII-activated phosphatase activity of Escherichia coli NRII (NtrB): how the different domains of NRII collaborate to act as a phosphatase. Biochemistry. 2003;42:8885-99 pubmed
  9. Jiang P, Peliska J, Ninfa A. The regulation of Escherichia coli glutamine synthetase revisited: role of 2-ketoglutarate in the regulation of glutamine synthetase adenylylation state. Biochemistry. 1998;37:12802-10 pubmed
    ..factor NRI (NtrC) in a reconstituted bicyclic system containing NRI, the bifunctional kinase/phosphatase NRII (NtrB), PII, and the UTase/UR...

More Information

Publications48

  1. Jiang P, Atkinson M, Srisawat C, Sun Q, Ninfa A. Functional dissection of the dimerization and enzymatic activities of Escherichia coli nitrogen regulator II and their regulation by the PII protein. Biochemistry. 2000;39:13433-49 pubmed
    The dimeric two-component system transmitter protein NRII (NtrB) of Escherichia coli, product of glnL (ntrB), controls transcription of nitrogen-regulated genes by catalyzing the phosphorylation and dephosphorylation of the transcription ..
  2. Atkinson M, Ninfa A. Mutational analysis of the bacterial signal-transducing protein kinase/phosphatase nitrogen regulator II (NRII or NtrB). J Bacteriol. 1993;175:7016-23 pubmed
    The signal-transducing kinase/phosphatase nitrogen regulator II (NRII or NtrB) is required for the efficient positive and negative regulation of glnA, encoding glutamine synthetase, and the Ntr regulon in response to the availability of ..
  3. Reitzer L, Magasanik B. Expression of glnA in Escherichia coli is regulated at tandem promoters. Proc Natl Acad Sci U S A. 1985;82:1979-83 pubmed
    ..We also provide evidence that the function of the glnL product is to mediate the interconversion of NRI between a form capable of activating glnAp2 and an inactive form ..
  4. Ninfa A, Selinsky S, Perry N, Atkins S, Xiu Song Q, Mayo A, et al. Using two-component systems and other bacterial regulatory factors for the fabrication of synthetic genetic devices. Methods Enzymol. 2007;422:488-512 pubmed
    ..systems for synthetic biology applications and describes our use of the Escherichia coli NRI/NRII (NtrC/NtrB) two-component system for the construction of a synthetic genetic oscillator and toggle switch for E. coli...
  5. Verhamme D, Arents J, Postma P, Crielaard W, Hellingwerf K. Investigation of in vivo cross-talk between key two-component systems of Escherichia coli. Microbiology. 2002;148:69-78 pubmed
    ..Cross-talk towards NtrC did occur, however, in a ntrB deletion strain, upon joint activation of Pho, Ntr and Uhp...
  6. Miranda Rios J, Sanchez Pescador R, Urdea M, Covarrubias A. The complete nucleotide sequence of the glnALG operon of Escherichia coli K12. Nucleic Acids Res. 1987;15:2757-70 pubmed
    The nucleotide sequence of the E. coli glnALG operon has been determined. The glnL (ntrB) and glnG (ntrC) genes present a high homology, at the nucleotide and aminoacid levels, with the corresponding genes of Klebsiella pneumoniae...
  7. Jiang P, Peliska J, Ninfa A. Asymmetry in the autophosphorylation of the two-component regulatory system transmitter protein nitrogen regulator II of Escherichia coli. Biochemistry. 2000;39:5057-65 pubmed
    Autophosphorylation of the homodimeric two-component system transmitter protein nitrogen regulator II (NRII; also NtrB) of Escherichia coli is the first step in the activation of nitrogen-regulated (Ntr) gene transcription...
  8. Pioszak A, Ninfa A. Mutations altering the N-terminal receiver domain of NRI (NtrC) That prevent dephosphorylation by the NRII-PII complex in Escherichia coli. J Bacteriol. 2004;186:5730-40 pubmed
    ..The properties of these altered proteins are consistent with the hypothesis that the NRII-PII complex is not a true phosphatase but rather collaborates with NRI approximately P to bring about its dephosphorylation. ..
  9. Nohno T, Saito T, Hong J. Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ). Mol Gen Genet. 1986;205:260-9 pubmed
    ..There were two additional reading frames (glnP and glnQ) downstream of glnH sharing a common promoter. It was concluded that the glnP and glnQ proteins as well as the glnH protein are essential for glutamine permease activity...
  10. Backman K, Chen Y, Magasanik B. Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1981;78:3743-7 pubmed
    ..A region of DNA between glnA and glnG contains genetic information whose loss may result in the inability to reduce expression of glnA and other operons in response to ammonia (the GlnC phenotype). ..
  11. Pahel G, Rothstein D, Magasanik B. Complex glnA-glnL-glnG operon of Escherichia coli. J Bacteriol. 1982;150:202-13 pubmed
    ..The lower level of glnG product originating from transcription initiated at the glnA-distal promoter is not sufficient to activate histidase synthesis, but is sufficient to activate fully and to repress glnA expression. ..
  12. Salinas P, Contreras A. Identification and analysis of Escherichia coli proteins that interact with the histidine kinase NtrB in a yeast two-hybrid system. Mol Genet Genomics. 2003;269:574-81 pubmed
    ..coli Sau3AI Y2H libraries using the nitrogen regulator NtrB as bait...
  13. Sasse Dwight S, Gralla J. Probing the Escherichia coli glnALG upstream activation mechanism in vivo. Proc Natl Acad Sci U S A. 1988;85:8934-8 pubmed
    ..These observations place interesting restrictions on models describing the mechanism by which NRI activates transcription from glnAp2 at a distance. ..
  14. Mutalik V, Venkatesh K. A theoretical steady state analysis indicates that induction of Escherichia coli glnALG operon can display all-or-none behavior. Biosystems. 2007;90:1-19 pubmed
    ..coli glnALG operon to nutrient starvation prevents the premature initiation of transcription and may represent the desperate attempt by the cell to survive in limiting conditions. ..
  15. Kramer G, Weiss V. Functional dissection of the transmitter module of the histidine kinase NtrB in Escherichia coli. Proc Natl Acad Sci U S A. 1999;96:604-9 pubmed
    ..To determine the mechanism of this switch, we analyzed the domain organization of the bifunctional histidine kinase NtrB. Based on sequence alignments with other histidine kinases and a deletion analysis, we defined three separate ..
  16. Atkinson M, Ninfa A. Characterization of Escherichia coli glnL mutations affecting nitrogen regulation. J Bacteriol. 1992;174:4538-48 pubmed
    Nitrogen regulator II (NRII), the product of the Escherichia coli glnL (ntrB) gene, regulates the activation of transcription of glnA and the Ntr regulon by catalyzing the phosphorylation and dephosphorylation of the transcription factor ..
  17. Ninfa E, Atkinson M, Kamberov E, Ninfa A. Mechanism of autophosphorylation of Escherichia coli nitrogen regulator II (NRII or NtrB): trans-phosphorylation between subunits. J Bacteriol. 1993;175:7024-32 pubmed
    Nitrogen regulator II (NRII or NtrB) is a homodimeric signal-transducing protein kinase/phosphatase responsible for the transcriptional regulation of the Ntr regulon in Escherichia coli...
  18. Ueno Nishio S, Mango S, Reitzer L, Magasanik B. Identification and regulation of the glnL operator-promoter of the complex glnALG operon of Escherichia coli. J Bacteriol. 1984;160:379-84 pubmed
    We sequenced the 274-nucleotide intercistronic glnA-glnL region of Escherichia coli to localize regulatory regions postulated from genetic evidence...
  19. McFarland N, McCarter L, Artz S, Kustu S. Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their protein products. Proc Natl Acad Sci U S A. 1981;78:2135-9 pubmed
    The nitrogen regulatory locus "glnR" of Escherichia coli and Salmonella typhimurium is composed of two cistrons, which we propose to call ntrB and ntrC (nitrogen regulation B and C)...
  20. Blauwkamp T, Ninfa A. Physiological role of the GlnK signal transduction protein of Escherichia coli: survival of nitrogen starvation. Mol Microbiol. 2002;46:203-14 pubmed
    ..The specific roles of GlnK during nitrogen starvation were not the result of a distinct function of the protein, as expression of PII from the glnK promoter in cells lacking GlnK restored the wild-type phenotypes. ..
  21. Burillo S, Luque I, Fuentes I, Contreras A. Interactions between the nitrogen signal transduction protein PII and N-acetyl glutamate kinase in organisms that perform oxygenic photosynthesis. J Bacteriol. 2004;186:3346-54 pubmed
  22. Jiang P, Ventura A, Ninfa A. Characterization of the reconstituted UTase/UR-PII-NRII-NRI bicyclic signal transduction system that controls the transcription of nitrogen-regulated (Ntr) genes in Escherichia coli. Biochemistry. 2012;51:9045-57 pubmed publisher
  23. Jiang P, Ninfa A. Alpha-ketoglutarate controls the ability of the Escherichia coli PII signal transduction protein to regulate the activities of NRII (NrB but does not control the binding of PII to NRII. Biochemistry. 2009;48:11514-21 pubmed publisher
    ..regulation was obtained with another PII receptor, the two-component system transmitter protein NRII (NtrB)...
  24. Ninfa A, Ueno Nishio S, Hunt T, Robustell B, Magasanik B. Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli. J Bacteriol. 1986;168:1002-4 pubmed
    We purified the product of glnL (ntrB), NRII, and the product of a mutant glnL allele, NRII2302...
  25. Chen Y, Backman K, Magasanik B. Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli. J Bacteriol. 1982;150:214-20 pubmed
    ..This region defines a gene, glnL, which codes for a trans-acting product; the GlnC mutant produces an altered product...
  26. Martinez Argudo I, Contreras A. PII T-loop mutations affecting signal transduction to NtrB also abolish yeast two-hybrid interactions. J Bacteriol. 2002;184:3746-8 pubmed
    ..of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J...
  27. Atkinson M, Ninfa A. Characterization of the GlnK protein of Escherichia coli. Mol Microbiol. 1999;32:301-13 pubmed
    ..glutamine synthetase adenylyltransferase (ATase) and the kinase/phosphatase nitrogen regulator II (NRII or NtrB), which controls transcription from nitrogen-regulated promoters...
  28. Weiss V, Kramer G, D√ľnnebier T, Flotho A. Mechanism of regulation of the bifunctional histidine kinase NtrB in Escherichia coli. J Mol Microbiol Biotechnol. 2002;4:229-33 pubmed
    b>NtrB is the bifunctional histidine kinase for nitrogen regulation...
  29. MacNeil T, Roberts G, MacNeil D, Tyler B. The products of glnL and glnG are bifunctional regulatory proteins. Mol Gen Genet. 1982;188:325-33 pubmed
  30. Song Y, Peisach D, Pioszak A, Xu Z, Ninfa A. Crystal structure of the C-terminal domain of the two-component system transmitter protein nitrogen regulator II (NRII; NtrB), regulator of nitrogen assimilation in Escherichia coli. Biochemistry. 2004;43:6670-8 pubmed
    The kinase/phosphatase nitrogen regulator II (NRII, NtrB) is a member of the transmitter protein family of conserved two-component signal transduction systems...
  31. Yang X, Ji Y, Schneider B, Reitzer L. Phosphorylation-independent dimer-dimer interactions by the enhancer-binding activator NtrC of Escherichia coli: a third function for the C-terminal domain. J Biol Chem. 2004;279:36708-14 pubmed
    ..We discuss the differences and similarities between the NtrC C-terminal domain and the homologous Fis, which is also capable of intermolecular interactions. ..
  32. Baick J, Yoon J, Namgoong S, Soll D, Kim S, Eom S, et al. Growth inhibition of Escherichia coli during heterologous expression of Bacillus subtilis glutamyl-tRNA synthetase that catalyzes the formation of mischarged glutamyl-tRNA1 Gln. J Microbiol. 2004;42:111-6 pubmed
    ..coli GlutRNA1 Gln, and converts it to the cognate Gln-tRNA1 Gln species. B. subtilis GluRS-dependent Glu-tRNA1 Gln formation may cause growth inhibition in the transformed E. coli strain, possibly due to abnormal protein synthesis. ..
  33. Kamberov E, Atkinson M, Chandran P, Ninfa A. Effect of mutations in Escherichia coli glnL (ntrB), encoding nitrogen regulator II (NRII or NtrB), on the phosphatase activity involved in bacterial nitrogen regulation. J Biol Chem. 1994;269:28294-9 pubmed
    We examined the effects of mutations in glnL, encoding the signal-transducing kinase/phosphatase nitrogen regulator II (NRII), on the regulated phosphatase activity involved in nitrogen regulation...
  34. Reitzer L. Nitrogen assimilation and global regulation in Escherichia coli. Annu Rev Microbiol. 2003;57:155-76 pubmed
    ..Guanosine tetraphosphate appears to control Lrp synthesis. In summary, a network of interacting global regulators that senses different aspects of metabolism integrates nitrogen assimilation with other metabolic processes. ..
  35. Ueno Nishio S, Backman K, Magasanik B. Regulation at the glnL-operator-promoter of the complex glnALG operon of Escherichia coli. J Bacteriol. 1983;153:1247-51 pubmed
    ..We identified the portion of the glnALG operon that contains the promoter and operator of the glnLG suboperon and showed that the product of glnG represses transcription initiated at glnLp. ..
  36. Rocha M, Vazquez M, Garciarrubio A, Covarrubias A. Nucleotide sequence of the glnA-glnL intercistronic region of Escherichia coli. Gene. 1985;37:91-9 pubmed
    ..nt) sequence of a 682-bp fragment containing the 3' end of the glnA gene, the region between the glnA and glnL genes, and the 5' end of the glnL gene from Escherichia coli was determined...
  37. Jiang P, Ninfa A. Sensation and signaling of alpha-ketoglutarate and adenylylate energy charge by the Escherichia coli PII signal transduction protein require cooperation of the three ligand-binding sites within the PII trimer. Biochemistry. 2009;48:11522-31 pubmed publisher
  38. MacNeil T, MacNeil D, Tyler B. Fine-structure deletion map and complementation analysis of the glnA-glnL-glnG region in Escherichia coli. J Bacteriol. 1982;150:1302-13 pubmed
    ..Mutations in Reg- strains were assigned to two genes, glnL and glnG...
  39. Magasanik B. Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation. Biochimie. 1989;71:1005-12 pubmed
    ..The signal is transduced to the modulator by means of 2 additional proteins: uridylytransferase and PII. ..