Gene Symbol: glk
Description: glucokinase
Alias: ECK2384, JW2385
Species: Escherichia coli str. K-12 substr. MG1655
Products:     glk

Top Publications

  1. Asensio C, Sols A. Utilization and phosphorylation of sugars by Escherichia coli. Rev Esp Fisiol. 1958;14:269-75 pubmed
  2. Bettenbrock K, Fischer S, Kremling A, Jahreis K, Sauter T, Gilles E. A quantitative approach to catabolite repression in Escherichia coli. J Biol Chem. 2006;281:2578-84 pubmed
    ..The different phenomena affecting the phosphorylation level of EIIACrr, the key regulation molecule for inducer exclusion and catabolite repression in enteric bacteria, can now be explained quantitatively. ..
  3. Zhang X, Jantama K, Moore J, Jarboe L, Shanmugam K, Ingram L. Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli. Proc Natl Acad Sci U S A. 2009;106:20180-5 pubmed publisher
    ..This glucose transport function was replaced by increased expression of the GalP permease (galP) and glucokinase (glk)...
  4. Fukuda Y, Yamaguchi S, Shimosaka M, Murata K, Kimura A. Cloning of the glucokinase gene in Escherichia coli B. J Bacteriol. 1983;156:922-5 pubmed
    A gene for glucokinase (Glk) in Escherichia coli B was cloned onto vector plasmid pBR322, and the hybrid plasmid obtained was designated pGK100. The gene for Glk was located in the central MluI fragment (0.82 megadalton) of the 6...
  5. Miller B. The mutability of enzyme active-site shape determinants. Protein Sci. 2007;16:1965-8 pubmed
    ..These findings establish non-hydrogen bonding shape determinants as highly appealing targets for widespread substitution during efforts to redesign the catalytic properties of natural enzymes. ..
  6. Asensio C, Avigad G, Horecker B. PREFERENTIAL GALACTOSE UTILIZATION IN A MUTANT STRAIN OF E. COLI. Arch Biochem Biophys. 1963;103:299-309 pubmed
  7. Hernández Montalvo V, Martinez A, Hernández Chávez G, Bolivar F, Valle F, Gosset G. Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products. Biotechnol Bioeng. 2003;83:687-94 pubmed was to study the effect of different gene expression levels, of galactose permease (GalP) and glucokinase (Glk), on glucose internalization and phosphorylation in a E. coli PTS(-) strain...
  8. Curtis S, Epstein W. Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. J Bacteriol. 1975;122:1189-99 pubmed
    ..The locus of mutations to loss of mannosephosphotransferase, mpt, is between the eda and fadD genes. Mutations to loss of glucokinase, glk, are between the ptsI and dsd genes.
  9. Oh M, Liao J. DNA microarray detection of metabolic responses to protein overproduction in Escherichia coli. Metab Eng. 2000;2:201-9 pubmed
    ..Upon induction, these strains shared several common responses, such as the upregulation of glk and the heat shock genes as well as the downregulation of fba, ppc, atpA, and gnd...

More Information


  2. Lengsfeld C, Schönert S, Dippel R, Boos W. Glucose- and glucokinase-controlled mal gene expression in Escherichia coli. J Bacteriol. 2009;191:701-12 pubmed publisher
    ..We report here a novel aspect of mal gene regulation: the effect of cytoplasmic glucose and glucokinase (Glk) on the activity and the expression of MalT. Amylomaltase (MalQ) is essential for the metabolism of maltose...
  3. Ogawa T, Mori H, Tomita M, Yoshino M. Inhibitory effect of phosphoenolpyruvate on glycolytic enzymes in Escherichia coli. Res Microbiol. 2007;158:159-63 pubmed
    ..PEP, a potent inhibitor of E. coli glucokinase, unlike most eukaryotic hexokinases, can act as a signal molecule controlling glucose uptake and glycolytic flux in cells. ..
  4. Lunin V, Li Y, Schrag J, Iannuzzi P, Cygler M, Matte A. Crystal structures of Escherichia coli ATP-dependent glucokinase and its complex with glucose. J Bacteriol. 2004;186:6915-27 pubmed
    ..200/0.271 and to 2.2-A resolution (glucose complex) with final Rwork/Rfree factors of 0.193/0.265. E. coli GlK is a homodimer of 321 amino acid residues...
  5. Miller B, Raines R. Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases. Biochemistry. 2004;43:6387-92 pubmed
    ..Our findings suggest that modern genomes harbor a wealth of latent enzyme activities and that extant metabolic pathways are equivocal, in contrast to their usual depiction. ..
  6. Arora K, Pedersen P. Glucokinase of Escherichia coli: induction in response to the stress of overexpressing foreign proteins. Arch Biochem Biophys. 1995;319:574-8 pubmed
    ..coli proteins, function in the stabilization of newly synthesized proteins. ..
  7. Brun Y, Breton R, Lanouette P, Lapointe J. Precise mapping and comparison of two evolutionarily related regions of the Escherichia coli K-12 chromosome. Evolution of valU and lysT from an ancestral tRNA operon. J Mol Biol. 1990;214:825-43 pubmed the 52 minute region has permitted precise mapping of most of the 18 genes in this region with the order nupC-glk- less than (alaW beta-ala W alpha)-1 kb- less than gltX-0...
  8. Ishii N, Suga Y, Hagiya A, Watanabe H, Mori H, Yoshino M, et al. Dynamic simulation of an in vitro multi-enzyme system. FEBS Lett. 2007;581:413-20 pubmed
    ..coli glycolytic enzymes. Thus, the results indicate that kinetic parameters can be determined without using an undesirable tuning process. ..
  9. Kawai S, Mukai T, Mori S, Mikami B, Murata K. Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family. J Biosci Bioeng. 2005;99:320-30 pubmed
  10. Wang Q, Wu C, Chen T, Chen X, Zhao X. Expression of galactose permease and pyruvate carboxylase in Escherichia coli ptsG mutant increases the growth rate and succinate yield under anaerobic conditions. Biotechnol Lett. 2006;28:89-93 pubmed
    ..The ptsG mutant (TUQ2), from wild type E. coli W1485, and E. coli galP (encoding galactose permease) and glk (encoding glucose kinase) gene expression plasmids were constructed...
  11. Siddiquee K, Arauzo Bravo M, Shimizu K. Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli. FEMS Microbiol Lett. 2004;235:25-33 pubmed
    ..RT-PCR revealed that the glycolytic genes such as glk, pgi, pfkA and tpiA were down regulated, that ppc, pckA, maeB and mdh genes were strongly up-regulated, and that ..
  12. Meyer D, Schneider Fresenius C, Horlacher R, Peist R, Boos W. Molecular characterization of glucokinase from Escherichia coli K-12. J Bacteriol. 1997;179:1298-306 pubmed
    b>glk, the structural gene for glucokinase of Escherichia coli, was cloned and sequenced. Overexpression of glk resulted in the synthesis of a cytoplasmic protein with a molecular weight of 35,000...