Gene Symbol: glgB
Description: 1,4-alpha-glucan branching enzyme
Alias: ECK3418, JW3395
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Abad M, Binderup K, Rios Steiner J, Arni R, Preiss J, Geiger J. The X-ray crystallographic structure of Escherichia coli branching enzyme. J Biol Chem. 2002;277:42164-70 pubmed
    ..It appears that there are two binding modes for oligosaccharides in these structures depending on the identity and location of this aromatic residue. ..
  2. Mikkelsen R, Binderup K, Preiss J. Tyrosine residue 300 is important for activity and stability of branching enzyme from Escherichia coli. Arch Biochem Biophys. 2001;385:372-7 pubmed
    ..Y300F also showed lower relative activity at elevated temperatures compared to wild-type. Thus, these results show that Tyr residue 300 in E. coli branching enzyme is important for activity and thermostability of the enzyme. ..
  3. Binderup K, Preiss J. Glutamate-459 is important for Escherichia coli branching enzyme activity. Biochemistry. 1998;37:9033-7 pubmed
    ..Changing Glu-459 to Ala, Lys, or Gln lowered the specific activities and altered the preferred substrate from amylose to amylopectin. ..
  4. Boyer C, Preiss J. Biosynthesis of bacterial glycogen. Purification and properties of the Escherichia coli b alpha-1,4,-glucan: alpha-1,4-glucan 6-glycosyltansferase. Biochemistry. 1977;16:3693-9 pubmed
  5. Boyer L, Roussel X, Courseaux A, Ndjindji O, Lancelon Pin C, Putaux J, et al. Expression of Escherichia coli glycogen branching enzyme in an Arabidopsis mutant devoid of endogenous starch branching enzymes induces the synthesis of starch-like polyglucans. Plant Cell Environ. 2016;39:1432-47 pubmed publisher
    ..Here, we have substituted the activity of BEs in Arabidopsis with that of the Escherichia coli glycogen BE (GlgB)...
  6. Binderup K, Mikkelsen R, Preiss J. Limited proteolysis of branching enzyme from Escherichia coli. Arch Biochem Biophys. 2000;377:366-71 pubmed
    ..Furthermore, a branching enzyme with 112 residues deleted at the amino terminal was constructed by recombinant technology and found to have properties identical to those of the proteolyzed enzyme. ..
  7. Romeo T, Kumar A, Preiss J. Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes. Gene. 1988;70:363-76 pubmed
    The nucleotide sequences of the Escherichia coli genome between the glycogen biosynthetic genes glgB and glgC, and 1170 bp of DNA which follows glgA have been determined...
  8. Guan H, Li P, Imparl Radosevich J, Preiss J, Keeling P. Comparing the properties of Escherichia coli branching enzyme and maize branching enzyme. Arch Biochem Biophys. 1997;342:92-8 pubmed
    ..The significance of minimum chain-length requirement by SBE is discussed in setting the invariant size of amylopectin cluster size (9 nm). ..
  9. Romeo T, Moore J, Smith J. A simple method for cloning genes involved in glucan biosynthesis: isolation of structural and regulatory genes for glycogen synthesis in Escherichia coli. Gene. 1991;108:23-9 pubmed
    ..The method could be made specific for a desired glg gene by using a recipient strain that is defective in the gene of interest. ..

More Information


  1. Devillers C, Piper M, Ballicora M, Preiss J. Characterization of the branching patterns of glycogen branching enzyme truncated on the N-terminus. Arch Biochem Biophys. 2003;418:34-8 pubmed
  2. Wang L, Regina A, Butardo V, Kosar Hashemi B, Larroque O, Kahler C, et al. Influence of in situ progressive N-terminal is still controversial truncation of glycogen branching enzyme in Escherichia coli DH5α on glycogen structure, accumulation, and bacterial viability. BMC Microbiol. 2015;15:96 pubmed publisher
    ..A series of Escherichia coli DH5α mutants were created with glgB genes that were in situ progressively N-terminus truncated...
  3. Salmond G, Lutkenhaus J, Donachie W. Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell envelope gene murG. J Bacteriol. 1980;144:438-40 pubmed
    ..This group is in a larger cluster of at least 10 genes, all of which are involved in some way with cell envelope growth. ..
  4. Binderup K, Mikkelsen R, Preiss J. Truncation of the amino terminus of branching enzyme changes its chain transfer pattern. Arch Biochem Biophys. 2002;397:279-85 pubmed
    ..native or truncated branching enzyme were individually expressed in a branching enzyme-deficient mutant, AC71 (glgB(-))...
  5. Abad M, Binderup K, Preiss J, Geiger J. Crystallization and preliminary X-ray diffraction studies of Escherichia coli branching enzyme. Acta Crystallogr D Biol Crystallogr. 2002;58:359-61 pubmed
    ..44, b = 102.58, c = 185.41 A, beta = 91.38 degrees. A native data set with 99.6% completeness, an overall R(merge) of 0.086 and I/sigma(I) of 10.43 was obtained. ..
  6. Latil Damotte M, Lares C. Relative order of glg mutations affecting glycogen biosynthesis in Escherichia coli K12. Mol Gen Genet. 1977;150:325-8 pubmed
    ..coli can be mapped at three different loci, glg A, glg B and glg C lying between asd and mal A. Transduction tests suggest the following order for the genes in this region: mal A--glg A--glg C--glg B--asd. ..
  7. Baecker P, Greenberg E, Preiss J. Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-D-(1, 4-alpha-D-glucano)-transferase as deduced from the nucleotide sequence of the glg B gene. J Biol Chem. 1986;261:8738-43 pubmed
    ..The deduced amino acid sequence was also consistent with the amino-terminal amino acid sequence and the amino acid sequence analysis of various peptides obtained from CNBr degradation of purified branching enzyme. ..
  8. Binderup K, Libessart N, Preiss J. Slow-binding inhibition of branching enzyme by the pseudooligosaccharide BAY e4609. Arch Biochem Biophys. 2000;374:73-8 pubmed
    ..We discuss how these results parallel the inhibition of alpha-amylase by acarbose and the significance of branching enzyme as belonging to the amylolytic family. ..
  9. Hilden I, Leggio L, Larsen S, Poulsen P. Characterization and crystallization of an active N-terminally truncated form of the Escherichia coli glycogen branching enzyme. Eur J Biochem. 2000;267:2150-5 pubmed