galP

Summary

Gene Symbol: galP
Description: D-galactose transporter
Alias: ECK2938, JW2910, Pgal
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Roy S, Lim H, Liu M, Adhya S. Asynchronous basepair openings in transcription initiation: CRP enhances the rate-limiting step. EMBO J. 2004;23:869-75 pubmed
    ..cAMP.CRP, which activates transcription at galP1, enhanced the rate-limiting step. ..
  2. Martin G, Seamon K, Brown F, Shanahan M, Roberts P, Henderson P. Forskolin specifically inhibits the bacterial galactose-H+ transport protein, GalP. J Biol Chem. 1994;269:24870-7 pubmed
    ..Here we show that forskolin is a remarkably specific inhibitor of energized D-galactose transport by the GalP sugar-H+ symport protein of Escherichia coli...
  3. Steel A, Cairns M, Walmsley A, Henderson P. Mutagenesis of the galactose-H+ symporter, GalP, of Escherichia coli. Biochem Soc Trans. 1994;22:277S pubmed
  4. Martin G, Rutherford N, Henderson P, Walmsley A. Kinetics and thermodynamics of the binding of forskolin to the galactose-H+ transport protein, GalP, of Escherichia coli. Biochem J. 1995;308 ( Pt 1):261-8 pubmed
    The binding of the transport inhibitor, forskolin, to the galactose-H+ symporter, GalP, of Escherichia coli was evaluated by equilibrium and time-resolved fluorescence measurements...
  5. Ward A, O Reilly J, Rutherford N, Ferguson S, Hoyle C, Palmer S, et al. Expression of prokaryotic membrane transport proteins in Escherichia coli. Biochem Soc Trans. 1999;27:893-9 pubmed
  6. Zhang X, Jantama K, Moore J, Jarboe L, Shanmugam K, Ingram L. Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli. Proc Natl Acad Sci U S A. 2009;106:20180-5 pubmed publisher
    ..This glucose transport function was replaced by increased expression of the GalP permease (galP) and glucokinase (glk)...
  7. Walmsley A, Martin G, Henderson P. 8-Anilino-1-naphthalenesulfonate is a fluorescent probe of conformational changes in the D-galactose-H+ symport protein of Escherichia coli. J Biol Chem. 1994;269:17009-19 pubmed
    The binding of sugars and antibiotics to the overexpressed D-galactose-H+ symport protein (GalP) can be monitored from changes in the fluorescence of 8-anilino-1-naphthalenesulfonate (ANS) equilibrated with inside-out vesicles...
  8. McDonald T, Henderson P. Cysteine residues in the D-galactose-H+ symport protein of Escherichia coli: effects of mutagenesis on transport, reaction with N-ethylmaleimide and antibiotic binding. Biochem J. 2001;353:709-17 pubmed
    The galactose-H(+) membrane-transport protein, GalP, of Escherichia coli is similar in substrate specificity and susceptibility to cytochalasin B and forskolin, to the human GLUT1 sugar-transport protein; furthermore, they are about 30% ..
  9. Cairns M, McDonald T, Horne P, Henderson P, Baldwin S. Cytochalasin B as a probe of protein structure and substrate recognition by the galactose/H+ transporter of Escherichia coli. J Biol Chem. 1991;266:8176-83 pubmed
    ..suggested that cytochalasin B might be a useful tool for investigation of the galactose/H+ symport protein (GalP) of Escherichia coli. Equilibrium binding studies using membranes from a GalP-constitutive (GalPc) strain of E...

More Information

Publications37

  1. Sanderson N, Martin G, Rutherford N, Henderson P. Purification, reconstitution and circular dichroism of the galactose-H+ transport protein [GalP-(His)6] of Escherichia coli. Biochem Soc Trans. 1997;25:471S pubmed
  2. Henderson P, Giddens R, Jones Mortimer M. Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. Biochem J. 1977;162:309-20 pubmed
    ..K., from whom copies can be obtained on the terms indicated in Biochem. J. (1977), 161,1. ..
  3. Patching S, Henderson P, Herbert R, Middleton D. Solid-state NMR spectroscopy detects interactions between tryptophan residues of the E. coli sugar transporter GalP and the alpha-anomer of the D-glucose substrate. J Am Chem Soc. 2008;130:1236-44 pubmed publisher
    ..provided insight into the binding site for the substrate D-glucose in the Escherichia coli sugar transport protein GalP. Cross-polarization magic-angle spinning (CP-MAS) SSNMR spectra of GalP in its natural membrane at 4 degrees C ..
  4. McDonald T, Walmsley A, Martin G, Henderson P. The role of tryptophans 371 and 395 in the binding of antibiotics and the transport of sugars by the D-galactose-H+ symport protein (GalP) from Escherichia coli. J Biol Chem. 1995;270:30359-70 pubmed
    The interactions between the D-galactose-H+ symporter (GalP) from Escherichia coli and the inhibitory antibiotics, cytochalasin B and forskolin, and the substrates, D-galactose and H+, have been investigated for the wild-type protein and ..
  5. El Qaidi S, Allemand F, Oberto J, Plumbridge J. Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. Mol Microbiol. 2009;71:146-57 pubmed publisher
    ..We have found that NagC, binds to a single, high-affinity site overlapping the promoter of galP (galactose permease) gene and that expression of galP is repressed by a combination of NagC, GalR and GalS...
  6. Appleyard A, Herbert R, Henderson P, Watts A, Spooner P. Selective NMR observation of inhibitor and sugar binding to the galactose-H(+) symport protein GalP, of Escherichia coli. Biochim Biophys Acta. 2000;1509:55-64 pubmed
    ..of the transport inhibitor forskolin, synthetically labelled with (13)C, to the galactose-H(+) symport protein GalP, overexpressed in its native inner membranes from Escherichia coli, was studied using cross-polarization magic ..
  7. Henderson P, Maiden M. Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes. Philos Trans R Soc Lond B Biol Sci. 1990;326:391-410 pubmed
    ..for D-xylose/H+ symport (XylE), L-arabinose/H+ symport (AraE), and part of the protein for D-galactose/H+ symport (GalP) have been determined...
  8. Wang Q, Wu C, Chen T, Chen X, Zhao X. Expression of galactose permease and pyruvate carboxylase in Escherichia coli ptsG mutant increases the growth rate and succinate yield under anaerobic conditions. Biotechnol Lett. 2006;28:89-93 pubmed
    ..The ptsG mutant (TUQ2), from wild type E. coli W1485, and E. coli galP (encoding galactose permease) and glk (encoding glucose kinase) gene expression plasmids were constructed...
  9. Patching S, Herbert R, O Reilly J, Brough A, Henderson P. Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli. J Am Chem Soc. 2004;126:86-7 pubmed
    ..This is largely solved for the bacterial galactose-H+ symport protein GalP by growing the producing organism Escherichia coli on 13C-depleted glucose (13C </= 0...
  10. Zheng H, Taraska J, Merz A, Gonen T. The prototypical H+/galactose symporter GalP assembles into functional trimers. J Mol Biol. 2010;396:593-601 pubmed publisher
    ..Galactose permease (GalP) is the closest bacterial homolog of human facilitated glucose transporters...
  11. Mowla R, Wang Y, Ma S, Venter H. Kinetic analysis of the inhibition of the drug efflux protein AcrB using surface plasmon resonance. Biochim Biophys Acta Biomembr. 2018;1860:878-886 pubmed publisher
    ..This article is part of a Special Issue entitled: Beyond the Structure-Function Horizon of Membrane Proteins edited by Ute Hellmich, Rupak Doshi and Benjamin McIlwain. ..
  12. McDonald T, Walmsley A, Henderson P. Asparagine 394 in putative helix 11 of the galactose-H+ symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar. J Biol Chem. 1997;272:15189-99 pubmed
    The galactose-H+ symport protein (GalP) of Escherichia coli is very similar to the human glucose transport protein, GLUT1, and both contain a highly conserved Asn residue in predicted helix 11 that is different in a cytochalasin B-..
  13. Venter H, Ashcroft A, Keen J, Henderson P, Herbert R. Molecular dissection of membrane-transport proteins: mass spectrometry and sequence determination of the galactose-H+ symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13C]histidine and (15)NH(3). Biochem J. 2002;363:243-52 pubmed
    The molecular mass of the galactose-H(+) symport protein GalP, as its histidine-tagged derivative GalP(His)(6), has been determined by electrospray MS (ESI-MS) with an error of <0.02%...
  14. Hubert A, Henderson P, Marsh D. Lipid-protein interactions in Escherichia coli membranes over-expressing the sugar-H(+) symporter, GalP EPR of spin-labelled lipids. Biochim Biophys Acta. 2003;1611:243-8 pubmed
    The D-galactose-H(+) symport protein (GalP) of Escherichia coli is a homologue of the human glucose transport protein, GLUT1. After amplified expression of the GalP transporter in E...
  15. MacPherson A, Jones Mortimer M, Horne P, Henderson P. Identification of the GalP galactose transport protein of Escherichia coli. J Biol Chem. 1983;258:4390-6 pubmed
    Escherichia coli strains have been isolated with a transposon 10 insertion or an amber mutation inactivating the galP gene, which specifies the galactose-H+ (GalP) transport system...
  16. Hernández Montalvo V, Martinez A, Hernández Chávez G, Bolivar F, Valle F, Gosset G. Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products. Biotechnol Bioeng. 2003;83:687-94 pubmed
    ..The purpose of this work was to study the effect of different gene expression levels, of galactose permease (GalP) and glucokinase (Glk), on glucose internalization and phosphorylation in a E. coli PTS(-) strain...
  17. Maiden M, Davis E, Baldwin S, Moore D, Henderson P. Mammalian and bacterial sugar transport proteins are homologous. Nature. 1987;325:641-3 pubmed
    ..There is little apparent homology with the lactose-H+ (LacY) or melibiose-Na+ (MelB) transport proteins of E. coli. ..
  18. Findlay H, Rutherford N, Henderson P, Booth P. Unfolding free energy of a two-domain transmembrane sugar transport protein. Proc Natl Acad Sci U S A. 2010;107:18451-6 pubmed publisher
    ..We determine the unfolding free energy of the two-domain, Escherichia coli galactose transporter, GalP; a bacterial homologue of human glucose transporters. GalP is reversibly unfolded by urea...
  19. Saidijam M, Bettaney K, Szakonyi G, Psakis G, Shibayama K, Suzuki S, et al. Active membrane transport and receptor proteins from bacteria. Biochem Soc Trans. 2005;33:867-72 pubmed
  20. Walmsley A, Lowe A, Henderson P. The kinetics and thermodynamics of the binding of cytochalasin B to sugar transporters. Eur J Biochem. 1994;221:513-22 pubmed
    The kinetics of the binding of cytochalasin B to the proton-linked L-arabinose (AraE) and D-galactose (GalP) symporters from Escherichia coli and to the human erythrocyte glucose transporter (GLUT1) have been investigated by exploiting ..
  21. Henderson P, McDonald T, Steel A, Litherland G, Cairns M, Martin G. The variability of kinetic parameters for sugar transport in different mutants of the galactose-H+ symport protein, GalP, of Escherichia coli. Biochem Soc Trans. 1994;22:643-6 pubmed
  22. Spooner P, Rutherford N, Watts A, Henderson P. NMR observation of substrate in the binding site of an active sugar-H+ symport protein in native membranes. Proc Natl Acad Sci U S A. 1994;91:3877-81 pubmed
    ..have been adopted to observe directly the characteristics of substrate binding to the galactose-H+ symport protein GalP, in its native environment, the inner membranes of Escherichia coli...
  23. Weickert M, Adhya S. The galactose regulon of Escherichia coli. Mol Microbiol. 1993;10:245-51 pubmed
    ..The gal regulon has the potential to coordinate galactose metabolism and transport in a highly efficient manner, under a wide variety of conditions of galactose availability. ..
  24. Henderson P, Martin G, McDonald T, Steel A, Walmsley A. Dissection of discrete kinetic events in the binding of antibiotics and substrates to the galactose-H+ symport protein, GalP, of Escherichia coli. Antonie Van Leeuwenhoek. 1994;65:349-58 pubmed
    b>GalP is the membrane protein responsible for H(+)-driven uptake of galactose into Escherichia coli...
  25. Martin G, Walmsley A, Henderson P. The interaction of forskolin with the galactose-H+ transport protein (GalP) of Escherichia coli. Biochem Soc Trans. 1994;22:278S pubmed
  26. Sanderson N, Qi D, Steel A, Henderson P. Effect of the D32N and N300F mutations on the activity of the bacterial sugar transport protein, GalP. Biochem Soc Trans. 1998;26:S306 pubmed
  27. Patching S, Psakis G, Baldwin S, Baldwin J, Henderson P, Middleton D. Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR. Mol Membr Biol. 2008;25:474-84 pubmed publisher
    ..time to examine the relative substrate-binding affinities of mutant forms of the Escherichia coli sugar transporter GalP in membrane preparations...
  28. Marsh D, Henderson P. Specific spin labelling of the sugar-H(+) symporter, GalP, in cell membranes of Escherichia coli: site mobility and overall rotational diffusion of the protein. Biochim Biophys Acta. 2001;1510:464-73 pubmed
    The D-galactose-H(+) symport protein (GalP) of Escherichia coli is a homologue of the human glucose transport protein, GLUT1. After amplified expression of the GalP transporter in E...