Gene Symbol: fumB
Description: anaerobic class I fumarate hydratase (fumarase B)
Alias: ECK4115, JW4083
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Kim O, Lux S, Unden G. Anaerobic growth of Escherichia coli on D-tartrate depends on the fumarate carrier DcuB and fumarase, rather than the L-tartrate carrier TtdT and L-tartrate dehydratase. Arch Microbiol. 2007;188:583-9 pubmed
    ..Deletion of fumB demonstrated, that fumarase B is required for growth on D-tartrate...
  2. Guest J, Roberts R. Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12. J Bacteriol. 1983;153:588-96 pubmed
    Two classes of fumarase-transducing phages, lambda fumA and lambda fumB, were isolated from populations of recombinant phages containing HindIII fragments of Escherichia coli DNA; they were isolated by virtue of their ability to ..
  3. Tseng C. Regulation of fumarase (fumB) gene expression in Escherichia coli in response to oxygen, iron and heme availability: role of the arcA, fur, and hemA gene products. FEMS Microbiol Lett. 1997;157:67-72 pubmed
    Three distinct fumarases, FumA, FumB and FumC, have been reported in Escherichia coli...
  4. Bell P, Andrews S, Sivak M, Guest J. Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12. J Bacteriol. 1989;171:3494-503 pubmed
    The nucleotide sequence of a 3,162-base-pair (bp) segment of DNA containing the FNR-regulated fumB gene, which encodes the anaerobic class I fumarase (FUMB) of Escherichia coli, was determined...
  5. Woods S, Schwartzbach S, Guest J. Two biochemically distinct classes of fumarase in Escherichia coli. Biochim Biophys Acta. 1988;954:14-26 pubmed
    ..with strains of Escherichia coli that are amplified for the products of the three fumarase genes, fumA (FUMA), fumB (FUMB) and fumC (FUMC), have shown that there are two distinct classes of fumarase...
  6. Flint D, Tuminello J, Emptage M. The inactivation of Fe-S cluster containing hydro-lyases by superoxide. J Biol Chem. 1993;268:22369-76 pubmed
    ..We report here that E. coli fumarase A, fumarase B, and aconitase are also inactivated in vivo by hyperbaric O2. Thermodynamic parameters for the oxidation of the cluster of aconitase by O2- and O2 are calculated. ..
  7. Tseng C, Yu C, Lin H, Chang C, Kuo J. Oxygen- and growth rate-dependent regulation of Escherichia coli fumarase (FumA, FumB, and FumC) activity. J Bacteriol. 2001;183:461-7 pubmed
    ..While FumB activity was maximal during anaerobic growth (k = 0...
  8. Park S, Gunsalus R. Oxygen, iron, carbon, and superoxide control of the fumarase fumA and fumC genes of Escherichia coli: role of the arcA, fnr, and soxR gene products. J Bacteriol. 1995;177:6255-62 pubmed
    ..Escherichia coli contains three biochemically distinct fumarases. While the fumA and fumB genes encode heat-labile, iron-containing fumarases, the fumC gene product is a heat-stable fumarase which does not ..
  9. Fischer E, Sauer U. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. J Biol Chem. 2003;278:46446-51 pubmed

More Information


  1. Golby P, Kelly D, Guest J, Andrews S. Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic C4-dicarboxylate transporters in Escherichia coli. J Bacteriol. 1998;180:6586-96 pubmed
    ..aspartase gene (aspA), while dcuB is 77 bp upstream of, and has the same polarity as, the anaerobic fumarase gene (fumB)...
  2. van Vugt Lussenburg B, van der Weel L, Hagen W, Hagedoorn P. Biochemical similarities and differences between the catalytic [4Fe-4S] cluster containing fumarases FumA and FumB from Escherichia coli. PLoS ONE. 2013;8:e55549 pubmed publisher
    The highly homologous [4Fe-4S] containing fumarases FumA and FumB, sharing 90% amino acid sequence identity, from Escherichia coli are differentially regulated, which suggests a difference in their physiological function...
  3. Herzberg M, Kaye I, Peti W, Wood T. YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport. J Bacteriol. 2006;188:587-98 pubmed
    ..metR), biotin and thiamine biosynthesis (bioF and thiDFH), anaerobic metabolism (focB, hyfACDR, ttdA, and fumB), and proteins with unknown function (ybfG, yceO, yjhQ, and yjbE); 10 of these genes were verified through mutation ..
  4. Abo Amer A, Munn J, Jackson K, Aktas M, Golby P, Kelly D, et al. DNA interaction and phosphotransfer of the C4-dicarboxylate-responsive DcuS-DcuR two-component regulatory system from Escherichia coli. J Bacteriol. 2004;186:1879-89 pubmed
    ..The DcuR-binding sites of the dcuB, dctA, and frdA genes were located 27, 94, and 86 bp, respectively, upstream of the corresponding +1 sites, and a new promoter was identified for dcuB that responds to DcuR. ..
  5. Flint D. Escherichia coli fumarase A catalyzes the isomerization of enol and keto oxalacetic acid. Biochemistry. 1993;32:799-805 pubmed
    ..Fumarase A and aconitase, two enzymes with 4Fe-4S clusters that bind a linear 4-carbon dicarboxylic acid moiety in the trans conformation during their normal hydro-lyase reaction, do catalyze this isomerization. ..
  6. Flint D. Initial kinetic and mechanistic characterization of Escherichia coli fumarase A. Arch Biochem Biophys. 1994;311:509-16 pubmed
    ..The possible roles of the proteins encoded by the three fumarase genes in E. coli are briefly discussed. ..
  7. Gruer M, Guest J. Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli. Microbiology. 1994;140 ( Pt 10):2531-41 pubmed
  8. Yumoto N, Tokushige M. Characterization of multiple fumarase proteins in Escherichia coli. Biochem Biophys Res Commun. 1988;153:1236-43 pubmed
    ..A. Woods & J.R. Guest (1987) FEMS Microbiol. Lett. 48, 219), the activity could be restored by anaerobic incubation with ferrous ions and SH-compounds. ..
  9. Guest J, Miles J, Roberts R, Woods S. The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC). J Gen Microbiol. 1985;131:2971-84 pubmed
    The fumB gene of Escherichia coli, which complements the fumarase deficiency of a fumA mutant when present in multiple copies, has been located at 93.5 min in the E...
  10. Henson J, Blake N, Marek L. The isolation of fumB mutants of Escherichia coli. J Gen Microbiol. 1987;133:2631-8 pubmed
    ..These suppressor mutants had an IS10 insertion just upstream of the fumB structural gene (min 93.4)...