Gene Symbol: ftsY
Description: Signal Recognition Particle (SRP) receptor
Alias: ECK3448, JW3429
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Parlitz R, Eitan A, Stjepanovic G, Bahari L, Bange G, Bibi E, et al. Escherichia coli signal recognition particle receptor FtsY contains an essential and autonomous membrane-binding amphipathic helix. J Biol Chem. 2007;282:32176-84 pubmed
    ..coli membrane protein biogenesis is mediated by a signal recognition particle and its membrane-associated receptor (FtsY). Although crucial for its function, it is still not clear how FtsY interacts with the membrane...
  2. Lu Y, Qi H, Hyndman J, Ulbrandt N, Teplyakov A, Tomasevic N, et al. Evidence for a novel GTPase priming step in the SRP protein targeting pathway. EMBO J. 2001;20:6724-34 pubmed
    ..SRP pathway GTPases, we mutated an invariant glycine in Escherichia coli SRP54 and SRalpha orthologs ('Ffh' and 'FtsY', respectively) that resides at the N-GTPase domain interface. A G257A mutation in Ffh produced a lethal phenotype...
  3. De Leeuw E, Poland D, Mol O, Sinning I, Ten Hagen Jongman C, Oudega B, et al. Membrane association of FtsY, the E. coli SRP receptor. FEBS Lett. 1997;416:225-9 pubmed
    b>FtsY, the Escherichia coli homologue of the eukaryotic SRP receptor (SR alpha), is located both in the cytoplasm and in the inner membrane of E. coli...
  4. Moser C, Mol O, Goody R, Sinning I. The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain. Proc Natl Acad Sci U S A. 1997;94:11339-44 pubmed
    ..to the inner membrane in Escherichia coli is achieved by the signal recognition particle (SRP) and its receptor (FtsY). In E. coli SRP consists of only one polypeptide (Ffh), and a 4.5S RNA...
  5. Ulbrandt N, Newitt J, Bernstein H. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins. Cell. 1997;88:187-96 pubmed
    ..coli preproteins and some IMPs can utilize SRP-independent targeting pathways effectively, the structural features of a subset of IMPs have required the conservation of an SRP-based targeting machinery. ..
  6. de Gier J, Mansournia P, Valent Q, Phillips G, Luirink J, von Heijne G. Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle. FEBS Lett. 1996;399:307-9 pubmed
    ..coli SRP. Depletion of either component strongly affected the insertion of both Lep and Lep-inv into the cytoplasmic membrane. This indicates that SRP is required for the assembly of cytoplasmic membrane proteins in E. coli. ..
  7. Egea P, Shan S, Napetschnig J, Savage D, Walter P, Stroud R. Substrate twinning activates the signal recognition particle and its receptor. Nature. 2004;427:215-21 pubmed
    ..This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery...
  8. Bradshaw N, Neher S, Booth D, Walter P. Signal sequences activate the catalytic switch of SRP RNA. Science. 2009;323:127-30 pubmed publisher
    ..Thus, SRP RNA is a molecular switch that renders the SRP-SR GTPase engine responsive to signal peptide recruitment, coupling GTP hydrolysis to productive protein targeting. ..
  9. Angelini S, Deitermann S, Koch H. FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. EMBO Rep. 2005;6:476-81 pubmed
    ..signal-recognition particle (SRP) requires the specific interaction of the SRP-ribosome nascent chain complex with FtsY, the bacterial SRP receptor (SR)...

More Information


  1. Zhang X, Schaffitzel C, Ban N, Shan S. Multiple conformational switches in a GTPase complex control co-translational protein targeting. Proc Natl Acad Sci U S A. 2009;106:1754-9 pubmed publisher
    ..Thus, the SRP and SR GTPases, without recruiting external regulatory factors, constitute a self-sufficient system that provides exquisite spatial and temporal control of a complex cellular process. ..
  2. Luirink J, Ten Hagen Jongman C, van der Weijden C, Oudega B, High S, Dobberstein B, et al. An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY. EMBO J. 1994;13:2289-96 pubmed
    ..In this study we have investigated the function of E. coli FtsY which shares sequence similarity with the alpha-subunit of the eukaryotic SRP receptor ('docking protein') in the ..
  3. Angelini S, Boy D, Schiltz E, Koch H. Membrane binding of the bacterial signal recognition particle receptor involves two distinct binding sites. J Cell Biol. 2006;174:715-24 pubmed
    Cotranslational protein targeting in bacteria is mediated by the signal recognition particle (SRP) and FtsY, the bacterial SRP receptor (SR)...
  4. Millman J, Andrews D. A site-specific, membrane-dependent cleavage event defines the membrane binding domain of FtsY. J Biol Chem. 1999;274:33227-34 pubmed
    ..However, the Escherichia coli signal recognition particle receptor, FtsY, differs significantly at the amino terminus from the eukaryote homologue alpha-subunit of the signal recognition ..
  5. Powers T, Walter P. Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases. Science. 1995;269:1422-4 pubmed
    The Escherichia coli guanosine triphosphate (GTP)-binding proteins Ffh and FtsY have been proposed to catalyze the cotranslational targeting of proteins to the bacterial plasma membrane...
  6. Mircheva M, Boy D, Weiche B, Hucke F, Graumann P, Koch H. Predominant membrane localization is an essential feature of the bacterial signal recognition particle receptor. BMC Biol. 2009;7:76 pubmed publisher
    ..The prokaryotic SR lacks the SRbeta subunit and consists of only the SRalpha homologue FtsY. Strikingly, although FtsY requires membrane contact for functionality, cell fractionation studies have localized ..
  7. Bornemann T, Jöckel J, Rodnina M, Wintermeyer W. Signal sequence-independent membrane targeting of ribosomes containing short nascent peptides within the exit tunnel. Nat Struct Mol Biol. 2008;15:494-9 pubmed publisher
    ..targeted to the translocon in the plasma membrane by the signal recognition particle (SRP) and the SRP receptor, FtsY. Here we show using a purified system that membrane targeting does not require an exposed signal-anchor sequence, ..
  8. Buskiewicz I, Jöckel J, Rodnina M, Wintermeyer W. Conformation of the signal recognition particle in ribosomal targeting complexes. RNA. 2009;15:44-54 pubmed publisher
    ..particle (SRP) binds to ribosomes synthesizing inner membrane proteins and, by interaction with the SRP receptor, FtsY, targets them to the translocon at the membrane...
  9. Herskovits A, Bochkareva E, Bibi E. New prospects in studying the bacterial signal recognition particle pathway. Mol Microbiol. 2000;38:927-39 pubmed
    ..The bacterial SRP system consists of at least two proteins and an RNA molecule (termed Ffh, FtsY and 4.5S RNA, respectively, in Escherichia coli)...
  10. Bernstein H, Poritz M, Strub K, Hoben P, Brenner S, Walter P. Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle. Nature. 1989;340:482-6 pubmed
    ..coli protein, ftsY, which resembles SR alpha...
  11. Bahari L, Parlitz R, Eitan A, Stjepanovic G, Bochkareva E, Sinning I, et al. Membrane targeting of ribosomes and their release require distinct and separable functions of FtsY. J Biol Chem. 2007;282:32168-75 pubmed
    The mechanism underlying the interaction of the Escherichia coli signal recognition particle (SRP) receptor FtsY with the cytoplasmic membrane is not fully understood...
  12. Peluso P, Shan S, Nock S, Herschlag D, Walter P. Role of SRP RNA in the GTPase cycles of Ffh and FtsY. Biochemistry. 2001;40:15224-33 pubmed
    ..5S RNA ribonucleoprotein complex and the FtsY protein, respectively, form a unique complex in which both Ffh and FtsY act as GTPase activating proteins for one ..
  13. Neher S, Bradshaw N, Floor S, Gross J, Walter P. SRP RNA controls a conformational switch regulating the SRP-SRP receptor interaction. Nat Struct Mol Biol. 2008;15:916-23 pubmed
    ..These results demonstrate that the N-terminal helices of SRP and SR are autoinhibitory for complex formation in the absence of SRP RNA, suggesting a mechanism for RNA-mediated coordination of the SRP-SR interaction. ..
  14. Jagath J, Rodnina M, Lentzen G, Wintermeyer W. Interaction of guanine nucleotides with the signal recognition particle from Escherichia coli. Biochemistry. 1998;37:15408-13 pubmed
    ..Binding of both GTP and GDP to Ffh was independent of Mg2+ ions. The data suggest that, at conditions in vivo, (i) there will be rapid spontaneous GDP-GTP exchange, and (ii) the GTP-bound form of Ffh, or of SRP, will be predominant. ..
  15. Braig D, Bär C, Thumfart J, Koch H. Two cooperating helices constitute the lipid-binding domain of the bacterial SRP receptor. J Mol Biol. 2009;390:401-13 pubmed publisher
    ..requires the specific interaction of the signal recognition particle (SRP)-ribosome-nascent chain complex with FtsY, the bacterial SRP receptor...
  16. Kusters R, Lentzen G, Eppens E, van Geel A, van der Weijden C, Wintermeyer W, et al. The functioning of the SRP receptor FtsY in protein-targeting in E. coli is correlated with its ability to bind and hydrolyse GTP. FEBS Lett. 1995;372:253-8 pubmed
    In this study, we have established that FtsY, the E. coli homolog of the mammalian signal recognition particle (SRP) receptor, is a GTP-binding protein which displays intrinsic GTPase activity...
  17. Powers T, Walter P. Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor. EMBO J. 1997;16:4880-6 pubmed
    The Ffh-4.5S ribonucleoprotein particle (RNP) and FtsY from Escherichia coli are homologous to essential components of the mammalian signal recognition particle (SRP) and SRP receptor, respectively. The ability of these E...
  18. Seluanov A, Bibi E. FtsY, the prokaryotic signal recognition particle receptor homologue, is essential for biogenesis of membrane proteins. J Biol Chem. 1997;272:2053-5 pubmed
    ..Unexpectedly, despite this variance, E. coli contains essential genes encoding Ffh and FtsY with a significant similarity to proteins of the eukaryotic SRP machinery...
  19. Koch H, Hengelage T, Neumann Haefelin C, Macfarlane J, Hoffschulte H, Schimz K, et al. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli. Mol Biol Cell. 1999;10:2163-73 pubmed
    ..5S RNA, and FtsY. The integration of two membrane proteins into inside-out plasma membrane vesicles of E...
  20. Tian H, Boyd D, Beckwith J. A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery. Proc Natl Acad Sci U S A. 2000;97:4730-5 pubmed
    ..Although both the ffs and the secA locus mutations interfere with membrane protein assembly, only the latter also reduces export of a protein containing a cleavable signal sequence. ..
  21. Shan S, Stroud R, Walter P. Mechanism of association and reciprocal activation of two GTPases. PLoS Biol. 2004;2:e320 pubmed
    ..During this process, two GTPases, one in SRP and one in the SRP receptor (named Ffh and FtsY in bacteria, respectively), form a complex in which both proteins reciprocally activate the GTPase reaction of one ..
  22. Muller M, Koch H, Beck K, Schafer U. Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane. Prog Nucleic Acid Res Mol Biol. 2001;66:107-57 pubmed
    ..occurs during synthesis at the ribosome and leads to a cotranslational targeting to SecYE that is mediated by FtsY and the hydrolysis of GTP...
  23. de Gier J, Luirink J. Biogenesis of inner membrane proteins in Escherichia coli. Mol Microbiol. 2001;40:314-22 pubmed
    ..Here, we review and discuss recent advances in our understanding of the biogenesis of inner membrane proteins in E. coli. ..
  24. de Gier J, Scotti P, Saaf A, Valent Q, Kuhn A, Luirink J, et al. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proc Natl Acad Sci U S A. 1998;95:14646-51 pubmed
    ..The observed change in assembly mechanism when the hydrophobicity of the procoat signal peptide is increased demonstrates that the assembly of an inner membrane protein can be rerouted between different pathways. ..
  25. De Leeuw E, te Kaat K, Moser C, Menestrina G, Demel R, De Kruijff B, et al. Anionic phospholipids are involved in membrane association of FtsY and stimulate its GTPase activity. EMBO J. 2000;19:531-41 pubmed
    b>FtsY, the Escherichia coli homologue of the eukaryotic signal recognition particle (SRP) receptor alpha-subunit, is located in both the cytoplasm and inner membrane...
  26. Macfarlane J, Muller M. The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal-recognition particle. Eur J Biochem. 1995;233:766-71 pubmed
    ..These results suggest a major function of the bacterial SRP in the targeting and subsequent integration of hydrophobic membrane proteins as opposed to SecA mediating the post-translational targeting of secretory proteins. ..
  27. Buskiewicz I, Deuerling E, Gu S, Jöckel J, Rodnina M, Bukau B, et al. Trigger factor binds to ribosome-signal-recognition particle (SRP) complexes and is excluded by binding of the SRP receptor. Proc Natl Acad Sci U S A. 2004;101:7902-6 pubmed
    ..Furthermore, we show that binding of the SRP receptor, FtsY, to ribosome-bound SRP excludes TF from the ribosome...
  28. Luirink J, von Heijne G, Houben E, de Gier J. Biogenesis of inner membrane proteins in Escherichia coli. Annu Rev Microbiol. 2005;59:329-55 pubmed
    ..This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. ..
  29. Eitan A, Bibi E. The core Escherichia coli signal recognition particle receptor contains only the N and G domains of FtsY. J Bacteriol. 2004;186:2492-4 pubmed
    ..domain (approximately 200 amino acid residues) of the Escherichia coli signal recognition particle (SRP) receptor, FtsY, is required for membrane targeting...
  30. Weiche B, Bürk J, Angelini S, Schiltz E, Thumfart J, Koch H. A cleavable N-terminal membrane anchor is involved in membrane binding of the Escherichia coli SRP receptor. J Mol Biol. 2008;377:761-73 pubmed publisher
    ..receptor lacks a membrane-tethering SRP receptor (SR) beta subunit and is composed of only the SR alpha homologue FtsY. FtsY is a modular protein composed of three domains...
  31. Montoya G, Svensson C, Luirink J, Sinning I. Crystal structure of the NG domain from the signal-recognition particle receptor FtsY. Nature. 1997;385:365-8 pubmed
    ..Here we report the structure of the GTPase-containing portion of FtsY, the functional homologue of the SRP receptor of Escherichia coli, at 2.2 A resolution without bound nucleotide...
  32. Peluso P, Herschlag D, Nock S, Freymann D, Johnson A, Walter P. Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor. Science. 2000;288:1640-3 pubmed
    ..5S RNA ribonucleoprotein complex (Ffh/4.5S RNP; the SRP) and the FtsY protein (the SRP receptor). We have quantified the effects of 4...
  33. van der Laan M, Bechtluft P, Kol S, Nouwen N, Driessen A. F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis. J Cell Biol. 2004;165:213-22 pubmed
    ..Remarkably, signal recognition particle and its membrane-bound receptor FtsY are not required for the membrane insertion of F0c. In conclusion, a novel membrane protein insertion pathway in E...
  34. van Bloois E, ten Hagen Jongman C, Luirink J. Flexibility in targeting and insertion during bacterial membrane protein biogenesis. Biochem Biophys Res Commun. 2007;362:727-33 pubmed
    ..The modified model IMPs were analyzed for altered routing using an in vivo protease mapping approach. The data suggest a facultative use of different combinations of factors. ..
  35. van Stelten J, Silva F, Belin D, Silhavy T. Effects of antibiotics and a proto-oncogene homolog on destruction of protein translocator SecY. Science. 2009;325:753-6 pubmed publisher
    ..Intriguingly, YccA is a functional homolog of the proto-oncogene product Bax Inhibitor-1, which may share a similar mechanism of action in regulating apoptosis upon prolonged secretion stress. ..
  36. Lam V, Akopian D, Rome M, Henningsen D, Shan S. Lipid activation of the signal recognition particle receptor provides spatial coordination of protein targeting. J Cell Biol. 2010;190:623-35 pubmed publisher
    ..that phospholipid binding drives important conformational rearrangements that activate the bacterial SRP receptor FtsY and the SRP-FtsY complex...
  37. Ataide S, Schmitz N, Shen K, Ke A, Shan S, Doudna J, et al. The crystal structure of the signal recognition particle in complex with its receptor. Science. 2011;331:881-6 pubmed publisher
    ..This rearrangement provides a mechanism for coupling GTP hydrolysis to the handover of cargo to the translocon. ..
  38. von Loeffelholz O, Jiang Q, Ariosa A, Karuppasamy M, Huard K, Berger I, et al. Ribosome-SRP-FtsY cotranslational targeting complex in the closed state. Proc Natl Acad Sci U S A. 2015;112:3943-8 pubmed publisher
    ..In Escherichia coli, the SRP and its receptor FtsY bind to ribosome-nascent chain complexes with signal sequences and undergo a series of distinct conformational ..
  39. Kuruma Y, Nishiyama K, Shimizu Y, Muller M, Ueda T. Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins. Biotechnol Prog. 2005;21:1243-51 pubmed
    ..integration of an inner membrane protein (MtlA) into U-INVs was achieved in the presence of only SRP (Ffh) and SR (FtsY)...
  40. Wood H, Luirink J, Tollervey D. Evolutionary conserved nucleotides within the E.coli 4.5S RNA are required for association with P48 in vitro and for optimal function in vivo. Nucleic Acids Res. 1992;20:5919-25 pubmed
    ..On the other hand, nucleotides within the single-stranded regions bulged from the stem were found to be important both for the binding of P48 to the RNA and for optimal function of the RNA in vivo. ..
  41. Yi L, Celebi N, Chen M, Dalbey R. Sec/SRP requirements and energetics of membrane insertion of subunits a, b, and c of the Escherichia coli F1F0 ATP synthase. J Biol Chem. 2004;279:39260-7 pubmed
    ..Taken together, the in vivo data suggest that subunits a and b are inserted by the Sec/SRP pathway with the help of YidC, and subunit c is integrated into the membrane by the novel YidC pathway. ..
  42. Maeda I, Hirose N, Yamashiro H, Ichibori A, Ohshima S, Fujimoto T, et al. Comparative study of the N-terminal hydrophilic region in Streptomyces lividans and E. coli FtsY. Curr Microbiol. 2003;47:22-5 pubmed
    Streptomyces lividans FtsY (SlFtsY) was cloned and overexpressed in Escherichia coli. Analysis of the amino acid (aa) sequence showed a concentration of hydrophilic aa's in the N-terminal half region of SlFtsY as observed in that of E...
  43. Zheng N, Gierasch L. Domain interactions in E. coli SRP: stabilization of M domain by RNA is required for effective signal sequence modulation of NG domain. Mol Cell. 1997;1:79-87 pubmed
    ..The other domain of Fth (NG) is a GTPase, which binds and is coordinately regulated by its receptor, FtsY. We find that the helical M domain is inherently flexible. Binding of 4...
  44. Suzuma S, Hayashi K, Nakamura K, Yamane K. Analysis of Escherichia coli 4.5S RNA binding affinity to Ffh and EF-G. FEMS Microbiol Lett. 1999;180:271-7 pubmed
    ..15 microM and 1.5 microM, respectively. Moreover, we also show that very little EF-G can form a stable complex with 4.5S RNA in vivo, whereas almost all Ffh binds to 4.5S RNA. ..
  45. Focia P, Gawronski Salerno J, Coon J, Freymann D. Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site. J Mol Biol. 2006;360:631-43 pubmed
    The signal recognition particle (SRP) GTPases Ffh and FtsY play a central role in co-translational targeting of proteins, assembling in a GTP-dependent manner to generate the SRP targeting complex at the membrane...
  46. Luirink J, High S, Wood H, Giner A, Tollervey D, Dobberstein B. Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex. Nature. 1992;359:741-3 pubmed
    ..Here we show that the P48/4.5S RNA ribonucleoprotein complex interacts specifically with the signal sequence of a nascent secretory protein and therefore is a signal recognition particle. ..
  47. Andersen E, Rosenblad M, Larsen N, Westergaard J, Burks J, Wower I, et al. The tmRDB and SRPDB resources. Nucleic Acids Res. 2006;34:D163-8 pubmed publisher
    ..All alignments can be easily examined using a new exploratory browser. The databases provide links to high-resolution structures and serve as depositories for structures obtained by molecular modeling...
  48. Yosef I, Bochkareva E, Adler J, Bibi E. Membrane protein biogenesis in Ffh- or FtsY-depleted Escherichia coli. PLoS ONE. 2010;5:e9130 pubmed publisher
    ..for biogenesis of membrane proteins and contains two essential proteins: the SRP subunit Ffh and the SRP-receptor FtsY. Scattered in vivo studies have raised the possibility that expression of membrane proteins is inhibited in cells ..
  49. Grudnik P, Bange G, Sinning I. Protein targeting by the signal recognition particle. Biol Chem. 2009;390:775-82 pubmed publisher
    ..SRP54 and FtsY, two multidomain proteins with guanosine triphosphatase (GTPase) activity, are the central elements of the SRP ..
  50. Dong H, Jiang J, Li Y. The distinct anchoring mechanism of FtsY from different microbes. Curr Microbiol. 2009;59:336-40 pubmed publisher
    The SRP receptor FtsY, which is involved in targeting and translocating membrane protein, is generally composed of the N-terminal domain and the NG domain...
  51. Tian H, Beckwith J. Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway. J Bacteriol. 2002;184:111-8 pubmed
    ..coli signal recognition particle pathway: ffh, ffs, and ftsY, which encode Ffh, 4.5S RNA, and FtsY, respectively...
  52. Breukink E. Cell biology. Lethal traffic jam. Science. 2009;325:684-5 pubmed publisher
  53. Kuhn P, Weiche B, Sturm L, Sommer E, Drepper F, Warscheid B, et al. The bacterial SRP receptor, SecA and the ribosome use overlapping binding sites on the SecY translocon. Traffic. 2011;12:563-78 pubmed publisher
    ..By site-directed cross-linking and fluorescence resonance energy transfer (FRET) analyses, we show that FtsY, the bacterial SR? homologue, binds to the exposed C4/C5 loops of SecY, the central component of the bacterial Sec ..
  54. Vicente M, Rico A. The order of the ring: assembly of Escherichia coli cell division components. Mol Microbiol. 2006;61:5-8 pubmed
    ..In addition, back-recruitment of all late proteins except FtsN into the division ring occurs even in the absence of proteins incorporated at earlier stages, i.e. FtsA or FtsQ. ..
  55. Marrichi M, Camacho L, Russell D, DeLisa M. Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria. J Biol Chem. 2008;283:35223-35 pubmed publisher
  56. Maeda I, Hirata A, Shoji M, Ueda S, Yoshida K. Functional substitution of the transient membrane-anchor domain in Escherichia coli FtsY with an N-terminal hydrophobic segment of Streptomyces lividans FtsY. FEMS Microbiol Lett. 2008;287:85-90 pubmed publisher
    b>FtsY is a signal recognition particle receptor in Escherichia coli that mediates the targeting of integral membrane proteins to translocons by interacting with both signal recognition particle (SRP)-nascent polypeptide-ribosome complexes ..
  57. Gill D, Hatfull G, Salmond G. A new cell division operon in Escherichia coli. Mol Gen Genet. 1986;205:134-45 pubmed
    ..This common domain is indicative of a nucleotide-binding site. ..
  58. Bürk J, Weiche B, Wenk M, Boy D, Nestel S, Heimrich B, et al. Depletion of the signal recognition particle receptor inactivates ribosomes in Escherichia coli. J Bacteriol. 2009;191:7017-26 pubmed publisher
    ..In the current study, we have analyzed how bacteria like Escherichia coli respond to a gradual depletion of FtsY, the bacterial SRP receptor...
  59. Chen S, Fan Y, Shen X, Sun P, Jiang G, Shen Y, et al. A molecular modeling study of the interaction between SRP-receptor complex and peptide translocon. Biochem Biophys Res Commun. 2008;377:346-350 pubmed publisher
    ..showing how nascent peptide inserts into peptide translocon with the help of SRP protein Ffh and its receptor FtsY, is desired to facilitate our studies...
  60. De Leeuw E, Graham B, Phillips G, Ten Hagen Jongman C, Oudega B, Luirink J. Molecular characterization of Escherichia coli FtsE and FtsX. Mol Microbiol. 1999;31:983-93 pubmed
    The genes ftsE and ftsX are organized in one operon together with ftsY. FtsY codes for the receptor of the signal recognition particle (SRP) that functions in targeting a subset of inner membrane proteins...
  61. Gill D, Salmond G. The Escherichia coli cell division proteins FtsY, FtsE and FtsX are inner membrane-associated. Mol Gen Genet. 1987;210:504-8 pubmed
    The cell division genes ftsY, ftsE and ftsX form an operon mapping at 76 min on the Escherichia coli chromosome. The protein products of these genes have been identified previously...
  62. Ezraty B, Grimaud R, El Hassouni M, Moinier D, Barras F. Methionine sulfoxide reductases protect Ffh from oxidative damages in Escherichia coli. EMBO J. 2004;23:1868-77 pubmed
    ..We conclude that MsrA and MsrB are required to repair Ffh oxidized by reactive oxygen species produced by aerobic metabolism, establishing an as-yet undescribed link between protein targeting and oxidation. ..
  63. Gill D, Salmond G. The identification of the Escherichia coli ftsY gene product: an unusual protein. Mol Microbiol. 1990;4:575-83 pubmed
    ..Although FtsE and FtsX have been identified using SDS-PAGE, the FtsY protein has not...
  64. Chu F, Shan S, Moustakas D, Alber F, Egea P, Stroud R, et al. Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry. Proc Natl Acad Sci U S A. 2004;101:16454-9 pubmed
    ..proteins to the bacterial plasma membrane, the specific interaction between Ffh (the protein component of SRP) and FtsY (the SRP receptor) is known to be essential for the efficiency and fidelity of this process...
  65. Buskiewicz I, Kubarenko A, Peske F, Rodnina M, Wintermeyer W. Domain rearrangement of SRP protein Ffh upon binding 4.5S RNA and the SRP receptor FtsY. RNA. 2005;11:947-57 pubmed
    ..a protein, Ffh, that recognizes the signal peptide emerging from the ribosome and the SRP receptor at the membrane, FtsY. In the present work, we studied the interactions between the NG and M domains in Ffh and their rearrangements upon ..
  66. Poritz M, Bernstein H, Strub K, Zopf D, Wilhelm H, Walter P. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle. Science. 1990;250:1111-7 pubmed
    ..Protein synthesis did not cease until very late, an indication that 4.5S RNA probably does not have a direct role in this process. ..