Gene Symbol: fimH
Description: minor component of type 1 fimbriae
Alias: ECK4311, JW4283
Species: Escherichia coli str. K-12 substr. MG1655
Products:     fimH

Top Publications

  1. Abraham S, Goguen J, Sun D, Klemm P, Beachey E. Identification of two ancillary subunits of Escherichia coli type 1 fimbriae by using antibodies against synthetic oligopeptides of fim gene products. J Bacteriol. 1987;169:5530-6 pubmed
    ..synthesized oligopeptides corresponding to the NH2-terminal stretch of two gene products, designated FimG and FimH, of the fim gene cluster of Escherichia coli...
  2. Munera D, Hultgren S, Fernández L. Recognition of the N-terminal lectin domain of FimH adhesin by the usher FimD is required for type 1 pilus biogenesis. Mol Microbiol. 2007;64:333-46 pubmed
    In this work we discover that a specific recognition of the N-terminal lectin domain of FimH adhesin by the usher FimD is essential for the biogenesis of type 1 pili in Escherichia coli...
  3. Le Trong I, Aprikian P, Kidd B, Forero Shelton M, Tchesnokova V, Rajagopal P, et al. Structural basis for mechanical force regulation of the adhesin FimH via finger trap-like beta sheet twisting. Cell. 2010;141:645-55 pubmed publisher
    The Escherichia coli fimbrial adhesive protein, FimH, mediates shear-dependent binding to mannosylated surfaces via force-enhanced allosteric catch bonds, but the underlying structural mechanism was previously unknown...
  4. Sokurenko E, Courtney H, Ohman D, Klemm P, Hasty D. FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes. J Bacteriol. 1994;176:748-55 pubmed
    ..In this report, we provide evidence that this functional heterogeneity is due to variations in the fimH genes...
  5. Saulino E, Bullitt E, Hultgren S. Snapshots of usher-mediated protein secretion and ordered pilus assembly. Proc Natl Acad Sci U S A. 2000;97:9240-5 pubmed
    ..Specialized pilin subunits play unique roles both in this multimerization and in the final ultrastructure of the adhesive pilus. ..
  6. Bouckaert J, Berglund J, Schembri M, De Genst E, Cools L, Wuhrer M, et al. Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin. Mol Microbiol. 2005;55:441-55 pubmed
    ..coli to uroplakin receptors in the uroepithelium via the FimH adhesin located at the tips of type 1 pili. Blocking of bacterial adhesion is able to prevent infection...
  7. Choudhury D, Thompson A, Stojanoff V, Langermann S, Pinkner J, Hultgren S, et al. X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli. Science. 1999;285:1061-6 pubmed most members of the Enterobacteriaceae family-mediate binding to mannose receptors on host cells through the FimH adhesin. Pilus biogenesis proceeds by way of the chaperone/usher pathway...
  8. Zhou G, Mo W, Sebbel P, Min G, Neubert T, Glockshuber R, et al. Uroplakin Ia is the urothelial receptor for uropathogenic Escherichia coli: evidence from in vitro FimH binding. J Cell Sci. 2001;114:4095-103 pubmed
    ..In addition, it triggers bacterial invasion as well as host urothelial defense. This binding is mediated by the FimH adhesin located at the tip of the bacterial type 1-fimbrium, a filamentous attachment apparatus, and its urothelial ..
  9. Hung C, Bouckaert J, Hung D, Pinkner J, Widberg C, DeFusco A, et al. Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection. Mol Microbiol. 2002;44:903-15 pubmed the colonization of the human urinary tract by pathogenic Escherichia coli is the mannose-sensitive binding of FimH, the adhesin present at the tip of type 1 pili, to the bladder epithelium...

More Information


  1. Sauer F, Remaut H, Hultgren S, Waksman G. Fiber assembly by the chaperone-usher pathway. Biochim Biophys Acta. 2004;1694:259-67 pubmed
    ..Chaperone dissociation releases the folding energy that drives subunit incorporation into the fiber, which grows through a pore formed by the outer-membrane usher. ..
  2. Thomas W, Nilsson L, Forero M, Sokurenko E, Vogel V. Shear-dependent 'stick-and-roll' adhesion of type 1 fimbriated Escherichia coli. Mol Microbiol. 2004;53:1545-57 pubmed true for Escherichia coli attachment to monomannose-coated surfaces via the type 1 fimbrial adhesive subunit, FimH. Raising the shear stress (within the physiologically relevant range) increased accumulation of type 1 fimbriated ..
  3. Westerlund Wikström B, Korhonen T. Molecular structure of adhesin domains in Escherichia coli fimbriae. Int J Med Microbiol. 2005;295:479-86 pubmed
    Crystal structures of FimH, PapG, GafD, and DraE fimbrial adhesin subunits or lectin domains have been resolved. These adhesins bind to different targets and are only distantly related in amino acid sequence...
  4. Hahn E, Wild P, Hermanns U, Sebbel P, Glockshuber R, Häner M, et al. Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J Mol Biol. 2002;323:845-57 pubmed
    ..In situ immuno-electron microscopy experiments showed the minor subunit (FimH) mediating pilus adhesion to bladder epithelial cells to be the distal protein of the pilus tip, which had a spring-..
  5. Chen S, Hung C, Pinkner J, Walker J, Cusumano C, Li Z, et al. Positive selection identifies an in vivo role for FimH during urinary tract infection in addition to mannose binding. Proc Natl Acad Sci U S A. 2009;106:22439-44 pubmed publisher
    b>FimH, the type 1 pilus adhesin of uropathogenic Escherichia coli (UPEC), contains a receptor-binding domain with an acidic binding pocket specific for mannose...
  6. Jones C, Pinkner J, Roth R, Heuser J, Nicholes A, Abraham S, et al. FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae. Proc Natl Acad Sci U S A. 1995;92:2081-5 pubmed
    ..One complex contained only the mannose-binding adhesin, FimH, associated with FimG, a minor component of the type 1 pilus...
  7. Schembri M, Hasman H, Klemm P. Expression and purification of the mannose recognition domain of the FimH adhesin. FEMS Microbiol Lett. 2000;188:147-51 pubmed
    ..These structural organelles mediate specific adhesion to alpha-D-mannosides by virtue of the FimH adhesin...
  8. Nilsson L, Thomas W, Trintchina E, Vogel V, Sokurenko E. Catch bond-mediated adhesion without a shear threshold: trimannose versus monomannose interactions with the FimH adhesin of Escherichia coli. J Biol Chem. 2006;281:16656-63 pubmed
    The FimH protein is the adhesive subunit of Escherichia coli type 1 fimbriae...
  9. Munera D, Palomino C, Fernández L. Specific residues in the N-terminal domain of FimH stimulate type 1 fimbriae assembly in Escherichia coli following the initial binding of the adhesin to FimD usher. Mol Microbiol. 2008;69:911-25 pubmed publisher
    ..b>FimH adhesin initiates and stimulates type 1 fimbriae polymerization by interacting with FimD...
  10. Barnhart M, Sauer F, Pinkner J, Hultgren S. Chaperone-subunit-usher interactions required for donor strand exchange during bacterial pilus assembly. J Bacteriol. 2003;185:2723-30 pubmed
    ..We discovered that the information necessary for targeting the FimC-FimH complex to the usher resides mainly in the FimH protein...
  11. Sule P, Wadhawan T, Carr N, Horne S, Wolfe A, Prüss B. A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants. Lett Appl Microbiol. 2009;49:299-304 pubmed publisher
    ..The ATP assay, in combination with other quantitative and qualitative assays, will allow us to perform genetic studies on the regulatory network that underlies the early steps in E. coli biofilm formation. ..
  12. Feenstra T, Thøgersen M, Wieser E, Peschel A, Ball M, Brandes R, et al. Adhesion of Escherichia coli under flow conditions reveals potential novel effects of FimH mutations. Eur J Clin Microbiol Infect Dis. 2017;36:467-478 pubmed publisher
    b>FimH-mediated adhesion of Escherichia coli to bladder epithelium is a prerequisite for urinary tract infections. FimH is also essential for blood-borne bacterial dissemination, but the mechanisms are poorly understood...
  13. Klemm P, Christiansen G. The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae. Mol Gen Genet. 1990;220:334-8 pubmed
    ..A deletion derivative of FimD gave clues as to which parts of the protein were necessary for outer membrane integration. ..
  14. Harris S, Elliott D, Blake M, Must L, Messenger M, Orndorff P. Isolation and characterization of mutants with lesions affecting pellicle formation and erythrocyte agglutination by type 1 piliated Escherichia coli. J Bacteriol. 1990;172:6411-8 pubmed
    The product of the pilE (also called fimH) gene is a minor component of type 1 pili in Escherichia coli...
  15. Sauer M, Jakob R, Eras J, Baday S, EriÅŸ D, Navarra G, et al. Catch-bond mechanism of the bacterial adhesin FimH. Nat Commun. 2016;7:10738 pubmed publisher
    ..These pathogens attach to host epithelia via the adhesin FimH, a two-domain protein at the tip of type I pili recognizing terminal mannoses on epithelial glycoproteins...
  16. Sokurenko E, Schembri M, Trintchina E, Kjaergaard K, Hasty D, Klemm P. Valency conversion in the type 1 fimbrial adhesin of Escherichia coli. Mol Microbiol. 2001;41:675-86 pubmed
    b>FimH protein is a lectin-like adhesive subunit of type 1, or mannose-sensitive, fimbriae that are found on the surface of most Escherichia coli strains. All naturally occurring FimH variants demonstrate a conserved mannotriose-specific (i...
  17. Krogfelt K, Bergmans H, Klemm P. Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae. Infect Immun. 1990;58:1995-8 pubmed
    ..By direct binding of FimH to D-mannose attached to a carrier protein, we demonstrated that this protein was uniquely responsible for the ..
  18. Nishiyama M, Horst R, Eidam O, Herrmann T, Ignatov O, Vetsch M, et al. Structural basis of chaperone-subunit complex recognition by the type 1 pilus assembly platform FimD. EMBO J. 2005;24:2075-86 pubmed
    ..Together with in vivo complementation studies, we show how this mechanism enables recognition and discrimination of different chaperone-subunit complexes by bacterial pilus assembly platforms. ..
  19. Khan N, Kim Y, Shin S, Kim K. FimH-mediated Escherichia coli K1 invasion of human brain microvascular endothelial cells. Cell Microbiol. 2007;9:169-78 pubmed
    ..Here, we demonstrate that type 1 fimbriae, specifically FimH adhesin is not only an adhesive organelle that provides bacteria with a foothold on brain endothelial cells but ..
  20. Daniels R, Normark S. Twin ushers guide pili across the bacterial outer membrane. Cell. 2008;133:574-6 pubmed publisher
    ..2008) present the crystal structure of the PapC usher translocation domain and images of the FimD usher bound to a pilus translocation intermediate. These new structures provide the first detailed view of a translocase in action. ..
  21. Pratt L, Kolter R. Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol. 1998;30:285-93 pubmed
    ..In addition, we present evidence that type I pili (harbouring the mannose-specific adhesin, FimH) are required for initial surface attachment and that mannose inhibits normal attachment...
  22. Hamrick T, Harris S, Spears P, Havell E, Horton J, Russell P, et al. Genetic characterization of Escherichia coli type 1 pilus adhesin mutants and identification of a novel binding phenotype. J Bacteriol. 2000;182:4012-21 pubmed
    Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized...
  23. Hanson M, Brinton C. Identification and characterization of E. coli type-1 pilus tip adhesion protein. Nature. 1988;332:265-8 pubmed
    ..This protein is antigenically conserved among strains with different pilin serotypes and is located at the pilus tip. ..
  24. Dorman C, Higgins C. Fimbrial phase variation in Escherichia coli: dependence on integration host factor and homologies with other site-specific recombinases. J Bacteriol. 1987;169:3840-3 pubmed
    ..A mechanism by which IHF may exert control over fimA expression is discussed. ..
  25. Gally D, Leathart J, Blomfield I. Interaction of FimB and FimE with the fim switch that controls the phase variation of type 1 fimbriae in Escherichia coli K-12. Mol Microbiol. 1996;21:725-38 pubmed
    ..Furthermore, the different binding affinities of FimB and FimE for the various half-site combinations suggests that the specificity of FimE could arise, in part, from the low affinity of FimE for IRL (off)...
  26. Remaut H, Tang C, Henderson N, Pinkner J, Wang T, Hultgren S, et al. Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell. 2008;133:640-52 pubmed publisher
    ..These structures capture the secretion of a virulence factor across the outer membrane of gram-negative bacteria...
  27. Lee J, Shin S, Teng C, Hong S, Kim K. FimH adhesin of Escherichia coli K1 type 1 fimbriae activates BV-2 microglia. Biochem Biophys Res Commun. 2005;334:917-23 pubmed
    ..In this study, we showed that FimH adhesin, a tip component of type 1 fimbriae of meningitis-causing Escherichia coli K1, activated the murine ..
  28. Weissman S, Beskhlebnaya V, Chesnokova V, Chattopadhyay S, Stamm W, Hooton T, et al. Differential stability and trade-off effects of pathoadaptive mutations in the Escherichia coli FimH adhesin. Infect Immun. 2007;75:3548-55 pubmed
    b>FimH is the tip adhesin of mannose-specific type 1 fimbriae of Escherichia coli, which are critical to the pathogenesis of urinary tract infections...
  29. Dove S, Smith S, Dorman C. Control of Escherichia coli type 1 fimbrial gene expression in stationary phase: a negative role for RpoS. Mol Gen Genet. 1997;254:13-20 pubmed
    ..These data suggest that RpoS has a negative regulatory role which may be indirect, at both the fimA and the fimB promoters. ..
  30. Ronald L, Yakovenko O, Yazvenko N, Chattopadhyay S, Aprikian P, Thomas W, et al. Adaptive mutations in the signal peptide of the type 1 fimbrial adhesin of uropathogenic Escherichia coli. Proc Natl Acad Sci U S A. 2008;105:10937-42 pubmed publisher
    ..we report that naturally occurring mutations in the SP of the adhesive, tip-associated subunit of type 1 fimbriae (FimH) are positively selected in uropathogenic Escherichia coli...
  31. Tchesnokova V, Aprikian P, Kisiela D, Gowey S, Korotkova N, Thomas W, et al. Type 1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli. Infect Immun. 2011;79:3895-904 pubmed publisher
    ..coli express type 1 fimbriae. The fimbrial tip-positioned adhesive protein FimH utilizes a shear force-enhanced, so-called catch-bond mechanism of interaction with its receptor, mannose, where ..
  32. Erilov D, Puorger C, Glockshuber R. Quantitative analysis of nonequilibrium, denaturant-dependent protein folding transitions. J Am Chem Soc. 2007;129:8938-9 pubmed
  33. Aprikian P, Tchesnokova V, Kidd B, Yakovenko O, Yarov Yarovoy V, Trinchina E, et al. Interdomain interaction in the FimH adhesin of Escherichia coli regulates the affinity to mannose. J Biol Chem. 2007;282:23437-46 pubmed
    b>FimH is a mannose-specific adhesin located on the tip of type 1 fimbriae of Escherichia coli that is capable of mediating shear-enhanced bacterial adhesion...
  34. Schembri M, Christiansen G, Klemm P. FimH-mediated autoaggregation of Escherichia coli. Mol Microbiol. 2001;41:1419-30 pubmed
    ..This binding is conferred by the minor fimbrial component FimH. In this study, we have used random mutagenesis to identify variants of the FimH adhesin that confer the ability of ..
  35. Liu Y, Mémet S, Saban R, Kong X, Aprikian P, Sokurenko E, et al. Dual ligand/receptor interactions activate urothelial defenses against uropathogenic E. coli. Sci Rep. 2015;5:16234 pubmed publisher
    ..This activation depends on a dual ligand/receptor system, one between FimH adhesin and uroplakin Ia and another between lipopolysaccharide and Toll-like receptor 4...
  36. McClain M, Blomfield I, Eberhardt K, Eisenstein B. Inversion-independent phase variation of type 1 fimbriae in Escherichia coli. J Bacteriol. 1993;175:4335-44 pubmed
  37. Touaibia M, Wellens A, Shiao T, Wang Q, Sirois S, Bouckaert J, et al. Mannosylated G(0) dendrimers with nanomolar affinities to Escherichia coli FimH. ChemMedChem. 2007;2:1190-201 pubmed
    ..showed these two families of mannosylated clusters as the best ligands known to date toward Escherichia coli K12 FimH with subnanomolar affinities. Tetramer 4 had a K(d) of 0.45 nM...
  38. Brument S, Sivignon A, Dumych T, Moreau N, Roos G, Gu rardel Y, et al. Thiazolylaminomannosides as potent antiadhesives of type 1 piliated Escherichia coli isolated from Crohn's disease patients. J Med Chem. 2013;56:5395-406 pubmed publisher
    ..We developed a set of mannosides to prevent AIEC attachment to the gut by blocking the FimH bacterial adhesin...
  39. Schwan W, Seifert H, Duncan J. Analysis of the fimB promoter region involved in type 1 pilus phase variation in Escherichia coli. Mol Gen Genet. 1994;242:623-30 pubmed
    ..This putative binding protein may be involved in the regulation of fimB, perhaps by acting as a transcriptional activator. ..
  40. Lowe M, Holt S, Eisenstein B. Immunoelectron microscopic analysis of elongation of type 1 fimbriae in Escherichia coli. J Bacteriol. 1987;169:157-63 pubmed
    ..The pattern of labeling on individual fimbrial organelles indicated morphologically that newly synthesized subunits are added to a growing organelle at its base. ..
  41. Vetsch M, Sebbel P, Glockshuber R. Chaperone-independent folding of type 1 pilus domains. J Mol Biol. 2002;322:827-40 pubmed
    ..The previously determined X-ray structure of the complex between the type 1 pilus adhesin FimH and the periplasmic pilus assembly chaperone FimC has shown that FimH consists of a N-terminal lectin domain and a ..
  42. Yakovenko O, Sharma S, Forero M, Tchesnokova V, Aprikian P, Kidd B, et al. FimH forms catch bonds that are enhanced by mechanical force due to allosteric regulation. J Biol Chem. 2008;283:11596-605 pubmed publisher
    The bacterial adhesive protein, FimH, is the most common adhesin of Escherichia coli and mediates weak adhesion at low flow but strong adhesion at high flow...
  43. Nilsson L, Yakovenko O, Tchesnokova V, Thomas W, Schembri M, Vogel V, et al. The cysteine bond in the Escherichia coli FimH adhesin is critical for adhesion under flow conditions. Mol Microbiol. 2007;65:1158-69 pubmed
    Cysteine bonds are found near the ligand-binding sites of a wide range of microbial adhesive proteins, including the FimH adhesin of Escherichia coli...
  44. Schembri M, Sokurenko E, Klemm P. Functional flexibility of the FimH adhesin: insights from a random mutant library. Infect Immun. 2000;68:2638-46 pubmed
    ..This binding is conferred by the minor fimbrial component FimH. Naturally occurring variants of the FimH protein have been selected in nature for their ability to recognize ..
  45. Iebba V, Conte M, Lepanto M, Di Nardo G, Santangelo F, Aloi M, et al. Microevolution in fimH gene of mucosa-associated Escherichia coli strains isolated from pediatric patients with inflammatory bowel disease. Infect Immun. 2012;80:1408-17 pubmed publisher
    ..The majority of E. coli strains possess type 1 fimbriae, whose tip fibrillum protein, FimH, naturally undergoes amino acid replacements, an important process in the adaptation of commensal E...
  46. Anderson B, Ding A, Nilsson L, Kusuma K, Tchesnokova V, Vogel V, et al. Weak rolling adhesion enhances bacterial surface colonization. J Bacteriol. 2007;189:1794-802 pubmed
    ..However, some bacteria, such as Escherichia coli when binding to mannosylated surfaces via the adhesive protein FimH, adhere weakly in a mode that allows them to roll across the surface...
  47. Shin S, Kim K. Human brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH. FEMS Microbiol Lett. 2010;303:156-62 pubmed publisher
    ..contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP synthase beta-..
  48. Ashkar A, Mossman K, Coombes B, Gyles C, MacKenzie R. FimH adhesin of type 1 fimbriae is a potent inducer of innate antimicrobial responses which requires TLR4 and type 1 interferon signalling. PLoS Pathog. 2008;4:e1000233 pubmed publisher
    ..We have recently shown that FimH, the adhesin portion of type 1 fimbria, can induce the innate immune system via TLR4...
  49. Hanson M, Hempel J, Brinton C. Purification of the Escherichia coli type 1 pilin and minor pilus proteins and partial characterization of the adhesin protein. J Bacteriol. 1988;170:3350-8 pubmed
    ..5K and 14.5K proteins. We have determined the amino acid composition and amino-terminal sequence of the adhesion protein. This sequence shows limited homology with the amino-terminal sequences of several E. coli pilins, including type 1. ..
  50. Gally D, Rucker T, Blomfield I. The leucine-responsive regulatory protein binds to the fim switch to control phase variation of type 1 fimbrial expression in Escherichia coli K-12. J Bacteriol. 1994;176:5665-72 pubmed
    ..It is proposed that wrapping of fim DNA around an Lrp complex is required to form a recombination-proficient structure. ..
  51. Shen X, Ren L, Teng Y, Zheng S, Yang X, Guo X, et al. Luteolin decreases the attachment, invasion and cytotoxicity of UPEC in bladder epithelial cells and inhibits UPEC biofilm formation. Food Chem Toxicol. 2014;72:204-11 pubmed publisher
    ..Meanwhile, obvious decreased expression of type 1 fimbriae adhesin fimH gene, lower bacterial surface hydrophobicity and swimming motility, were observed in luteolin-pretreated UPEC...
  52. Krogfelt K, Klemm P. Investigation of minor components of Escherichia coli type 1 fimbriae: protein chemical and immunological aspects. Microb Pathog. 1988;4:231-8 pubmed
    Three minor components of type 1 fimbriae, FimF, FimG and FimH have been characterized...
  53. Saulino E, Thanassi D, Pinkner J, Hultgren S. Ramifications of kinetic partitioning on usher-mediated pilus biogenesis. EMBO J. 1998;17:2177-85 pubmed
    ..The data support a model in which binding of the chaperone-adhesin complex to the usher stabilizes the usher in an assembly-competent conformation and allows initiation of pilus assembly. ..
  54. Mori H, Ito K. The Sec protein-translocation pathway. Trends Microbiol. 2001;9:494-500 pubmed
    ..The detailed protein interactions involved in these processes are being actively studied and a structural understanding of the protein-conducting channel has started to emerge. ..
  55. Klemm P, Christiansen G. Three fim genes required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Mol Gen Genet. 1987;208:439-45 pubmed
    Three novel fim genes of Escherichia coli, fimF, fimG and fimH, were characterized...
  56. Chu D, Blomfield I. Orientational control is an efficient control mechanism for phase switching in the E. coli fim system. J Theor Biol. 2007;244:541-51 pubmed
    ..The main reason for the efficiency of orientational control in regulation of fimbriation levels is that it keeps the system far from its steady state. ..
  57. Orndorff P, Devapali A, Palestrant S, Wyse A, Everett M, Bollinger R, et al. Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber. Infect Immun. 2004;72:1929-38 pubmed
    ..A minor component of the pilus fiber (the product of the fimH gene, termed the adhesin) mediates attachment to a variety of host cell molecules in a mannose inhibitable ..
  58. He S, Yuan Z, Zhu G. [Construction and characterization of type I fimbriae fimH deletion mutant from avian pathogenic Escherichia coli]. Wei Sheng Wu Xue Bao. 2008;48:252-6 pubmed
    ..operon gene clusters in the GenBank,we generated PCR products by using primers with the homologies extension of fimH gene to be deleted and template plasmid pKD3 carrying selectable antibiotic chloramphenicol resistance (cat) gene ..
  59. Blomfield I, McClain M, Eisenstein B. Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants. Mol Microbiol. 1991;5:1439-45 pubmed
    ..To increase the utility of the new deletion strains we also isolated recA derivatives of each mutant. These strains facilitate the isolation, characterization, and manipulation of cloned fimbriae genes from diverse sources. ..
  60. Kawula T, Orndorff P. Rapid site-specific DNA inversion in Escherichia coli mutants lacking the histonelike protein H-NS. J Bacteriol. 1991;173:4116-23 pubmed
    ..Contrary to one current view on how the H-NS protein might act to increase DNA inversion rate, we found no evidence to support the hypothesis that DNA supercoiling affected pilA promoter inversion. ..
  61. Klemm P. FimC, a chaperone-like periplasmic protein of Escherichia coli involved in biogenesis of type 1 fimbriae. Res Microbiol. 1992;143:831-8 pubmed
    ..The fimC gene was sequenced, and the deduced primary structure of the FimC protein was compared to other similar known proteins involved in the biogenesis of various fimbriae. ..
  62. Adiciptaningrum A, Blomfield I, Tans S. Direct observation of type 1 fimbrial switching. EMBO Rep. 2009;10:527-32 pubmed publisher
    ..Analysis of the moment of switching at sub-cell-cycle resolution revealed a correlation between fim switching and cell age, which challenges the traditional idea of phase variation as a random Poissonian phenomenon. ..
  63. Karam M, Oloomi M, Mahdavi M, Habibi M, Bouzari S. Assessment of immune responses of the flagellin (FliC) fused to FimH adhesin of Uropathogenic Escherichia coli. Mol Immunol. 2013;54:32-9 pubmed publisher
    ..In this study, the immunogenicity of flagellin (FliC) of UPEC strain in different vaccine combinations with FimH antigen of UPEC and conventional adjuvant Montanide ISA 206 was assessed...
  64. Abraham J, Freitag C, Clements J, Eisenstein B. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A. 1985;82:5724-7 pubmed
    ..This rearrangement involves an invertible 314-base-pair segment of DNA whose alternating orientation apparently results in the on-and-off activation of a promoter that determines the state of fimbrial expression. ..
  65. Tchesnokova V, Aprikian P, Yakovenko O, Larock C, Kidd B, Vogel V, et al. Integrin-like allosteric properties of the catch bond-forming FimH adhesin of Escherichia coli. J Biol Chem. 2008;283:7823-33 pubmed publisher
    b>FimH is the adhesive subunit of type 1 fimbriae of the Escherichia coli that is composed of a mannose-binding lectin domain and a fimbria-incorporating pilin domain...
  66. Russell P, Orndorff P. Lesions in two Escherichia coli type 1 pilus genes alter pilus number and length without affecting receptor binding. J Bacteriol. 1992;174:5923-35 pubmed
    ..In contrast to previous studies, we found that neither gene product was required for type 1 pilus receptor binding. ..
  67. Bouckaert J, Mackenzie J, de Paz J, Chipwaza B, Choudhury D, Zavialov A, et al. The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of Escherichia coli pathotypes. Mol Microbiol. 2006;61:1556-68 pubmed
    ..adhesion to the high-mannosylated uroplakin Ia glycoprotein receptors of bladder epithelium is mediated by the FimH adhesin...
  68. Forero M, Yakovenko O, Sokurenko E, Thomas W, Vogel V. Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. PLoS Biol. 2006;4:e298 pubmed
    ..3-1.5 microm in length) built from a helically coiled structural subunit, FimA, and an adhesive subunit, FimH, incorporated at the fimbrial tip...