fhuA

Summary

Gene Symbol: fhuA
Description: ferrichrome outer membrane transporter
Alias: ECK0149, JW0146, tonA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Ferguson A, Braun V, Fiedler H, Coulton J, Diederichs K, Welte W. Crystal structure of the antibiotic albomycin in complex with the outer membrane transporter FhuA. Protein Sci. 2000;9:956-63 pubmed
    ..conjugate albomycin and the siderophore phenylferricrocin, in complex with the active outer membrane transporter FhuA from Escherichia coli...
  2. Ferguson A, Ködding J, Walker G, Bos C, Coulton J, Diederichs K, et al. Active transport of an antibiotic rifamycin derivative by the outer-membrane protein FhuA. Structure. 2001;9:707-16 pubmed
    b>FhuA, an integral membrane protein of Escherichia coli, actively transports ferrichrome and the structurally related antibiotic albomycin across the outer membrane...
  3. Braun M, Endriss F, Killmann H, Braun V. In vivo reconstitution of the FhuA transport protein of Escherichia coli K-12. J Bacteriol. 2003;185:5508-18 pubmed
    The FhuA protein in the outer membrane of Escherichia coli actively transports ferrichrome and the antibiotics albomycin and rifamycin CGP 4832 and serves as a receptor for the phages T1, T5, and phi80 and for colicin M and microcin J25...
  4. de Cristóbal R, Solbiati J, Zenoff A, Vincent P, Salomon R, Yuzenkova J, et al. Microcin J25 uptake: His5 of the MccJ25 lariat ring is involved in interaction with the inner membrane MccJ25 transporter protein SbmA. J Bacteriol. 2006;188:3324-8 pubmed
    ..MccJ25 enters cells after binding to specific membrane transporters: FhuA in the outer membrane and SbmA in the inner membrane...
  5. Destoumieux Garzón D, Duquesne S, Peduzzi J, Goulard C, Desmadril M, Letellier L, et al. The iron-siderophore transporter FhuA is the receptor for the antimicrobial peptide microcin J25: role of the microcin Val11-Pro16 beta-hairpin region in the recognition mechanism. Biochem J. 2005;389:869-76 pubmed
    The role of the outer-membrane iron transporter FhuA as a potential receptor for the antimicrobial peptide MccJ25 (microcin J25) was studied through a series of in vivo and in vitro experiments...
  6. Braun V, Gross R, Koster W, Zimmermann L. Plasmid and chromosomal mutants in the iron(III)-aerobactin transport system of Escherichia coli. Use of streptonigrin for selection. Mol Gen Genet. 1983;192:131-9 pubmed
    ..The genes fhuC,D,B were required for uptake of ferric aerobactin. Mutations in the fhuA gene usually decreased expression of the subsequent fhuC,D,B genes to such an extent that growth on ferric ..
  7. Killmann H, Braun V. Conversion of the coprogen transport protein FhuE and the ferrioxamine B transport protein FoxA into ferrichrome transport proteins. FEMS Microbiol Lett. 1998;161:59-67 pubmed
    The FhuA protein of Escherichia coli K-12 transports ferrichrome and the structurally related antibiotic albomycin across the outer membrane and serves as a receptor for the phages T1, T5, and phi 80 and for colicin M...
  8. Howard S, Herrmann C, Stratilo C, Braun V. In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli. J Bacteriol. 2001;183:5885-95 pubmed
    The siderophore transport activities of the two outer membrane proteins FhuA and FecA of Escherichia coli require the proton motive force of the cytoplasmic membrane...
  9. Killmann H, Videnov G, Jung G, Schwarz H, Braun V. Identification of receptor binding sites by competitive peptide mapping: phages T1, T5, and phi 80 and colicin M bind to the gating loop of FhuA. J Bacteriol. 1995;177:694-8 pubmed
    Previously we proposed a transmembrane model of the FhuA receptor protein in the outer membrane of Escherichia coli...

More Information

Publications74

  1. Endriss F, Braun V. Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coli. J Bacteriol. 2004;186:4818-23 pubmed
    Precise deletions of cell surface-exposed loops of FhuA resulted in mutants of Escherichia coli with distinct phenotypes. Deletion of loop 3 or 11 inactivated ferrichrome transport activity...
  2. Sansom M. Membrane proteins: A tale of barrels and corks. Curr Biol. 1999;9:R254-7 pubmed
    Crystal structures have been solved for two bacterial outer membrane proteins, FhuA and FepA, which mediate active transport of chelated iron...
  3. Kadner R, Heller K, Coulton J, Braun V. Genetic control of hydroxamate-mediated iron uptake in Escherichia coli. J Bacteriol. 1980;143:256-64 pubmed
    ..and unable to utilize ferrichrome and its analogs, although able to respond to the hydroxamate rhodotorulic acid (FhuA phenotype)...
  4. Koster W, Braun V. Iron (III) hydroxamate transport into Escherichia coli. Substrate binding to the periplasmic FhuD protein. J Biol Chem. 1990;265:21407-10 pubmed
    ..Binding depended on transport across the outer membrane mediated by the FhuA receptor and the TonB protein...
  5. Braun V, Killmann H, Benz R. Energy-coupled transport through the outer membrane of Escherichia coli small deletions in the gating loop convert the FhuA transport protein into a diffusion channel. FEBS Lett. 1994;346:59-64 pubmed
    Active transport of Fe3+ as ferrichrome complex through the outer membrane of Escherichia coli is mediated by the FhuA outer membrane protein and the TonB-ExbB-ExbD protein complex in the cytoplasmic membrane...
  6. Kadner R, Heller K. Mutual inhibition of cobalamin and siderophore uptake systems suggests their competition for TonB function. J Bacteriol. 1995;177:4829-35 pubmed
    ..inhibit ferrichrome uptake in wild-type cells, in which the amount of the outer membrane ferrichrome transporter FhuA far exceeds that of the cobalamin transporter BtuB, CN-Cbl does inhibit ferrichrome uptake when BtuB is ..
  7. Molloy M, Herbert B, Slade M, Rabilloud T, Nouwens A, Williams K, et al. Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem. 2000;267:2871-81 pubmed
    ..Furthermore, we show that like the E. coli iron receptors FhuE and FhuA, the expression of YbiL is markedly increased by iron limitation, suggesting a putative role for this protein in ..
  8. Udho E, Jakes K, Finkelstein A. TonB-dependent transporter FhuA in planar lipid bilayers: partial exit of its plug from the barrel. Biochemistry. 2012;51:6753-9 pubmed publisher
    ..presence of urea, trypsin can cleave the amino-terminal 67 residues of the plug of the TonB-dependent transporter FhuA, as assessed by gel shift and mass spectrometry assays...
  9. Faraldo Gómez J, Smith G, Sansom M. Molecular dynamics simulations of the bacterial outer membrane protein FhuA: a comparative study of the ferrichrome-free and bound states. Biophys J. 2003;85:1406-20 pubmed
    b>FhuA is one of the more complex members of the superfamily of bacterial outer membrane proteins...
  10. Mdzinarashvili T, Khvedelidze M, Ivanova A, Mrevlishvili G, Kutateladze M, Balarjishvili N, et al. Biophysics of T5, IRA phages, Escherichia coli outer membrane protein FhuA and T5-FhuA interaction. Eur Biophys J. 2006;35:231-8 pubmed
    ..strain, we have shown that these phages need different receptors to penetrate into the bacterial cell precisely FhuA serves as receptor only for T5 phage...
  11. Carmel G, Hellstern D, Henning D, Coulton J. Insertion mutagenesis of the gene encoding the ferrichrome-iron receptor of Escherichia coli K-12. J Bacteriol. 1990;172:1861-9 pubmed
    The ferrichrome-iron receptor of Escherichia coli K-12 encoded by the fhuA gene is a multifunctional outer membrane receptor with an Mr of 78,000...
  12. Koedding J, Howard P, Kaufmann L, Polzer P, Lustig A, Welte W. Dimerization of TonB is not essential for its binding to the outer membrane siderophore receptor FhuA of Escherichia coli. J Biol Chem. 2004;279:9978-86 pubmed
    b>FhuA belongs to a family of specific siderophore transport systems located in the outer membrane of Escherichia coli...
  13. Braun M, Killmann H, Maier E, Benz R, Braun V. Diffusion through channel derivatives of the Escherichia coli FhuA transport protein. Eur J Biochem. 2002;269:4948-59 pubmed
    b>FhuA is a multifunctional protein in the outer membrane of Escherichia coli that actively transports [Fe3+]ferrichrome, the antibiotics albomycin and rifamycin CGP 4832, and mediates sensitivity of cells to the unrelated phages T5, T1, ..
  14. Burkhardt R, Braun V. Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins. Mol Gen Genet. 1987;209:49-55 pubmed
    ..This study completes the nucleotide sequence of the fhu operon which consists of the four genes fhuA fhuC fhuD fhuB arranged in this order on the E. coli chromosome and transcribed from fhuA to fhuB.
  15. Bellomio A, Vincent P, de Arcuri B, Farias R, Morero R. Microcin J25 has dual and independent mechanisms of action in Escherichia coli: RNA polymerase inhibition and increased superoxide production. J Bacteriol. 2007;189:4180-6 pubmed
    Microcin J25 (MccJ25) uptake by Escherichia coli requires the outer membrane receptor FhuA and the inner membrane proteins TonB, ExbD, ExbB, and SbmA...
  16. Braun V, Wolff H. Characterization of the receptor protein for phage T5 and colicin M in the outer membrane of E. coli B. FEBS Lett. 1973;34:77-80 pubmed
  17. Jacoboni I, Martelli P, Fariselli P, De Pinto V, Casadio R. Prediction of the transmembrane regions of beta-barrel membrane proteins with a neural network-based predictor. Protein Sci. 2001;10:779-87 pubmed
    ..In addition, protein topology is assigned on the basis of the location of the longest loops in the models. We propose this as a general method to fill the gap of the prediction of beta-barrel membrane proteins. ..
  18. Charan H, Kinzel J, Glebe U, Anand D, Garakani T, Zhu L, et al. Grafting PNIPAAm from ?-barrel shaped transmembrane nanopores. Biomaterials. 2016;107:115-23 pubmed publisher
    ..a class of transmembrane proteins and grow polymer chains from the ferric hydroxamate uptake protein component A (FhuA; a ?-barrel transmembrane protein of Escherichia coli)...
  19. Koster W, Braun V. Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the fhuB gene and identification of the protein. Mol Gen Genet. 1986;204:435-42 pubmed
    The genes fhuA, fhuC and fhuD encode products involved in the transport of ferrichrome into cells of Escherichia coli. The nucleotide sequence of an additional locus contiguous to fhuD and formerly designated fhuB was determined...
  20. Luckey M, Neilands J. Iron transport in Salmonella typhimurium LT-2: prevention, by ferrichrome, of adsorption of bacteriophages ES18 and ES18.h1 to a common cell envelope receptor. J Bacteriol. 1976;127:1036-7 pubmed
    ..h1 to cells of Salmonella typhimurium. Studies of albomycin-resistant mutants defective in both ferrichrome utilization and ES18.h1 adsorption suggest a sid gene may specify a receptor for both. ..
  21. Luckey M, Pollack J, Wayne R, Ames B, Neilands J. Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J Bacteriol. 1972;111:731-8 pubmed
    ..Genetic and physiological data indicate that S. typhimurium has several transport systems of high specificity for a variety of siderochromes produced by other microorganisms. ..
  22. Bonhivers M, Ghazi A, Boulanger P, Letellier L. FhuA, a transporter of the Escherichia coli outer membrane, is converted into a channel upon binding of bacteriophage T5. EMBO J. 1996;15:1850-6 pubmed
    The Escherichia coli outer membrane protein FhuA catalyzes the transport of Fe3+(-)ferrichrome and is the receptor of phage T5 and phi 80. The purified protein inserted into planar lipid bilayers showed no channel activity...
  23. Khursigara C, De Crescenzo G, Pawelek P, Coulton J. Kinetic analyses reveal multiple steps in forming TonB-FhuA complexes from Escherichia coli. Biochemistry. 2005;44:3441-53 pubmed
    b>FhuA, an outer membrane receptor of Escherichia coli, facilitates transport of hydroxamate siderophores and siderophore-antibiotic conjugates...
  24. James K, Hancock M, Moreau V, Molina F, Coulton J. TonB induces conformational changes in surface-exposed loops of FhuA, outer membrane receptor of Escherichia coli. Protein Sci. 2008;17:1679-88 pubmed publisher
    b>FhuA, outer membrane receptor of Escherichia coli, transports hydroxamate-type siderophores into the periplasm. Cytoplasmic membrane-anchored TonB transduces energy to FhuA to facilitate siderophore transport...
  25. Fecker L, Braun V. Cloning and expression of the fhu genes involved in iron(III)-hydroxamate uptake by Escherichia coli. J Bacteriol. 1983;156:1301-14 pubmed
    ..and complementation studies between plasmid fragments and chromosomal mutants at least four loci in the order fhuA fhuC fhuD fhuB were found. The genetic products were determined in maxicells and minicells...
  26. Coulton J, Mason P, DuBow M. Molecular cloning of the ferrichrome-iron receptor of Escherichia coli K-12. J Bacteriol. 1983;156:1315-21 pubmed
    ..This protein of Mr 78,000 is the product of the fhuA (previously called tonA) gene, located at 3.5 min on the E. coli chromosome...
  27. Etz H, Minh D, Schellack C, Nagy E, Meinke A. Bacterial phage receptors, versatile tools for display of polypeptides on the cell surface. J Bacteriol. 2001;183:6924-35 pubmed
    ..tag or multiple copies of the myc epitope were inserted into loops 4 and 5 of the ferrichrome and phage T5 receptor FhuA. Fluorescence-activated cell sorting analysis showed that peptides of up to 250 amino acids were efficiently ..
  28. Moeck G, Ratcliffe M, Coulton J. Topological analysis of the Escherichia coli ferrichrome-iron receptor by using monoclonal antibodies. J Bacteriol. 1995;177:6118-25 pubmed
    Ferrichrome-iron transport in Escherichia coli is initiated by the outer membrane receptor FhuA. Thirty-five anti-FhuA monoclonal antibodies (MAbs) were isolated to examine the surface accessibility of FhuA sequences and their ..
  29. Samsonov V, Samsonov V, Sineoky S. DcrA and dcrB Escherichia coli genes can control DNA injection by phages specific for BtuB and FhuA receptors. Res Microbiol. 2002;153:639-46 pubmed
    ..work it is demonstrated that the DcrA and DcrB proteins, coordinately with another outer membrane receptor protein, FhuA, are also involved in an early stage of development of a newly isolated virulent phage, C6...
  30. Dykhuizen D, Dean A. Evolution of specialists in an experimental microcosm. Genetics. 2004;167:2015-26 pubmed
    ..The fate of this balanced polymorphism was tracked over evolutionary time by monitoring the frequency of fhuA-, a linked neutral genetic marker that confers resistance to the bacteriophage T5...
  31. Khursigara C, De Crescenzo G, Pawelek P, Coulton J. Enhanced binding of TonB to a ligand-loaded outer membrane receptor: role of the oligomeric state of TonB in formation of a functional FhuA.TonB complex. J Biol Chem. 2004;279:7405-12 pubmed
    The ferric hydroxymate uptake (FhuA) receptor from Escherichia coli facilitates transport of siderophores ferricrocin and ferrichrome and siderophore-antibiotic conjugates such as albomycin and rifamycin CGP 4832...
  32. Socias S, Severinov K, Salomon R. The Ile13 residue of microcin J25 is essential for recognition by the receptor FhuA, but not by the inner membrane transporter SbmA. FEMS Microbiol Lett. 2009;301:124-9 pubmed publisher
    Entry of the peptide antibiotic microcin J25 (MccJ25) into target cells is mediated by the outer membrane receptor FhuA and the inner membrane protein SbmA. The latter also transports MccB17 into the cell cytoplasm...
  33. Boulanger P, le Maire M, Bonhivers M, Dubois S, Desmadril M, Letellier L. Purification and structural and functional characterization of FhuA, a transporter of the Escherichia coli outer membrane. Biochemistry. 1996;35:14216-24 pubmed
    The Escherichia coli outer membrane ferrichrome transporter FhuA was purified chromatographically in a neutral detergent (octyl glucoside or dodecyl maltoside). The amount of dodecyl maltoside bound to the protein (1.2 +/- 0...
  34. Braun V, Braun M. Active transport of iron and siderophore antibiotics. Curr Opin Microbiol. 2002;5:194-201 pubmed
    ..Crystal structures of two outer membrane transport proteins, FhuA and FepA, and biochemical data reveal strong long-range conformational changes of the proteins upon binding of Fe(3+..
  35. Hantke K. Cloning of the repressor protein gene of iron-regulated systems in Escherichia coli K12. Mol Gen Genet. 1984;197:337-41 pubmed
    ..The synthesis of the outer membrane proteins fiu, fepA, fecA, fhuA, fhuE and cir is derepressed at low iron concentrations in the medium or constitutive in a fur mutant...
  36. Killmann H, Braun V. An aspartate deletion mutation defines a binding site of the multifunctional FhuA outer membrane receptor of Escherichia coli K-12. J Bacteriol. 1992;174:3479-86 pubmed
    The FhuA protein of the outer membrane serves as a receptor for phages T5, T1, and phi 80, for colicin M, for the antibiotic albomycin, and for ferrichrome and related siderophores...
  37. Killmann H, Herrmann C, Torun A, Jung G, Braun V. TonB of Escherichia coli activates FhuA through interaction with the beta-barrel. Microbiology. 2002;148:3497-509 pubmed
    b>FhuA is a multifunctional protein in the outer membrane of Escherichia coli that actively transports Fe(3+)-ferrichrome and the antibiotics albomycin and rifamycin CGP 4832, and serves as a receptor for the unrelated phages T5, T1, phi80 ..
  38. Pawelek P, Croteau N, Ng Thow Hing C, Khursigara C, Moiseeva N, Allaire M, et al. Structure of TonB in complex with FhuA, E. coli outer membrane receptor. Science. 2006;312:1399-402 pubmed
    ..The outer membrane receptor FhuA from Escherichia coli mediates TonB-dependent import of ferrichrome. We report the 3...
  39. Braun V, Pramanik A, Gwinner T, Köberle M, Bohn E. Sideromycins: tools and antibiotics. Biometals. 2009;22:3-13 pubmed publisher
    ..Crystal structures of albomycin bound to the outer membrane transporter FhuA and the periplasmic binding protein FhuD have been determined...
  40. Killmann H, Braun V. Energy-dependent receptor activities of Escherichia coli K-12: mutated TonB proteins alter FhuA receptor activities to phages T5, T1, phi 80 and to colicin M. FEMS Microbiol Lett. 1994;119:71-6 pubmed
    The activity of the FhuA receptor in the outer membrane of Escherichia coli is dependent on the TonB, ExbB and ExbD proteins which are anchored to the cytoplasmic membrane...
  41. Ramakrishnan M, Pocanschi C, Kleinschmidt J, Marsh D. Association of spin-labeled lipids with beta-barrel proteins from the outer membrane of Escherichia coli. Biochemistry. 2004;43:11630-6 pubmed
    ..The outer membrane protein OmpA and the ferrichrome-iron receptor FhuA from the outer membrane of Escherichia coli were reconstituted in bilayers of dimyristoylphosphatidylglycerol...
  42. Clarke T, Tari L, Vogel H. Structural biology of bacterial iron uptake systems. Curr Top Med Chem. 2001;1:7-30 pubmed
    ..Elucidation of the structures of the Escherichia coli outer membrane siderophore transport proteins FepA and FhuA have provided the first insights into the conformational changes required for ligand transport through the ..
  43. Killmann H, Benz R, Braun V. Conversion of the FhuA transport protein into a diffusion channel through the outer membrane of Escherichia coli. EMBO J. 1993;12:3007-16 pubmed
    The FhuA receptor protein is involved in energy-coupled transport of Fe3+ via ferrichrome through the outer membrane of Escherichia coli...
  44. Borloo J, De Smet L, Vergauwen B, Van Beeumen J, Devreese B. A beta-galactosidase-based bacterial two-hybrid system to assess protein-protein interactions in the correct cellular environment. J Proteome Res. 2007;6:2587-95 pubmed
    ..These benefits demonstrate the relevance of the method as a powerful new tool in the broad spectrum of interaction assessment methods. ..
  45. Letellier L, Locher K, Plançon L, Rosenbusch J. Modeling ligand-gated receptor activity. FhuA-mediated ferrichrome efflux from lipid vesicles triggered by phage T5. J Biol Chem. 1997;272:1448-51 pubmed
    An in vitro assay of iron-ferrichrome translocation across the FhuA protein of outer membranes from Escherichia coli has been devised...
  46. Ferguson A, Hofmann E, Coulton J, Diederichs K, Welte W. Siderophore-mediated iron transport: crystal structure of FhuA with bound lipopolysaccharide. Science. 1998;282:2215-20 pubmed
    b>FhuA, the receptor for ferrichrome-iron in Escherichia coli, is a member of a family of integral outer membrane proteins, which, together with the energy-transducing protein TonB, mediate the active transport of ferric siderophores across ..
  47. Eisenhauer H, Shames S, Pawelek P, Coulton J. Siderophore transport through Escherichia coli outer membrane receptor FhuA with disulfide-tethered cork and barrel domains. J Biol Chem. 2005;280:30574-80 pubmed
    The hydroxamate siderophore receptor FhuA is a TonB-dependent outer membrane protein of Escherichia coli composed of a C-terminal 22-stranded beta-barrel occluded by an N-terminal globular cork domain...
  48. Ramakrishnan M, Qu J, Pocanschi C, Kleinschmidt J, Marsh D. Orientation of beta-barrel proteins OmpA and FhuA in lipid membranes. Chain length dependence from infrared dichroism. Biochemistry. 2005;44:3515-23 pubmed
    The outer-membrane proteins OmpA and FhuA of Escherichia coli are monomeric beta-barrels of widely differing size...
  49. Hoegy F, Celia H, Mislin G, Vincent M, Gallay J, Schalk I. Binding of iron-free siderophore, a common feature of siderophore outer membrane transporters of Escherichia coli and Pseudomonas aeruginosa. J Biol Chem. 2005;280:20222-30 pubmed publisher
    ..b>FhuA, the ferrichrome OMT in Escherichia coli, and FptA, the pyochelin (Pch) OMT in Pseudomonas aeruginosa, were both ..
  50. Braun V, Hantke K, Stauder W. Identification of the sid outer membrane receptor protein in Salmonella typhimurium SL1027. Mol Gen Genet. 1977;155:227-9 pubmed
    ..coli protein mediate translocation of the iron complex ferrichrome and the structurally analogous antibiotic albomycin through the outer membrane no cross-reactivity exists in binding the phages T5, T1 and ES18 or colicin M. ..
  51. Braun V. FhuA (TonA), the career of a protein. J Bacteriol. 2009;191:3431-6 pubmed publisher
  52. Plan on L, Janmot C, le Maire M, Desmadril M, Bonhivers M, Letellier L, et al. Characterization of a high-affinity complex between the bacterial outer membrane protein FhuA and the phage T5 protein pb5. J Mol Biol. 2002;318:557-69 pubmed publisher
    ..8 kDa) and the outer membrane iron-transporter FhuA. A histidine-tagged form of pb5 was overproduced and purified...
  53. Locher K, Rees B, Koebnik R, Mitschler A, Moulinier L, Rosenbusch J, et al. Transmembrane signaling across the ligand-gated FhuA receptor: crystal structures of free and ferrichrome-bound states reveal allosteric changes. Cell. 1998;95:771-8 pubmed
    b>FhuA protein facilitates ligand-gated transport of ferrichrome-bound iron across Escherichia coli outer membranes. X-ray analysis at 2.7 A resolution reveals two distinct conformations in the presence and absence of ferrichrome...
  54. Endriss F, Braun M, Killmann H, Braun V. Mutant analysis of the Escherichia coli FhuA protein reveals sites of FhuA activity. J Bacteriol. 2003;185:4683-92 pubmed
    The FhuA outer membrane protein of Escherichia coli actively transports ferrichrome, albomycin, and rifamycin CGP 4832, and confers sensitivity to microcin J25, colicin M, and the phages T1, T5, and phi80...
  55. Braun V, Schaller K, Wolff H. A common receptor protein for phage T5 and colicin M in the outer membrane of Escherichia coli B. Biochim Biophys Acta. 1973;323:87-97 pubmed
  56. Killmann H, Braun M, Herrmann C, Braun V. FhuA barrel-cork hybrids are active transporters and receptors. J Bacteriol. 2001;183:3476-87 pubmed
    The crystal structure of Escherichia coli FhuA reveals a beta-barrel domain that is closed by a globular cork domain...
  57. Carter D, Gagnon J, Damlaj M, Mandava S, Makowski L, Rodi D, et al. Phage display reveals multiple contact sites between FhuA, an outer membrane receptor of Escherichia coli, and TonB. J Mol Biol. 2006;357:236-51 pubmed
    The ferric hydroxamate uptake receptor FhuA from Escherichia coli transports siderophores across the outer membrane (OM)...
  58. Chakraborty R, Storey E, van der Helm D. Molecular mechanism of ferricsiderophore passage through the outer membrane receptor proteins of Escherichia coli. Biometals. 2007;20:263-74 pubmed
    ..The crystal structures of three outer membrane ferricsiderophore receptor proteins FepA, FhuA and FecA from Escherichia coli and two FpvA and FptA from Pseudomonas aeruginosa have recently been solved...
  59. Langenscheid J, Killmann H, Braun V. A FhuA mutant of Escherichia coli is infected by phage T1-independent of TonB. FEMS Microbiol Lett. 2004;234:133-7 pubmed
    Infection of Escherichia coli K-12 by phages T1 and phi 80 requires the FhuA outer membrane protein and the TonB protein. Mutations in the N-terminal globular domain close to the predicted channel in the beta-barrel of FhuA were created...
  60. Gaspar M, Santos M, Krauter K, Winkelmann G. Molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(A-B) deletion mutants of Escherichia coli. Biometals. 1999;12:209-18 pubmed
    ..This could be proven by using a delta (fhuA-B) E...
  61. Braun V, Endriss F. Energy-coupled outer membrane transport proteins and regulatory proteins. Biometals. 2007;20:219-31 pubmed
    b>FhuA and FecA are two examples of energy-coupled outer membrane import proteins of gram-negative bacteria...
  62. Sauter A, Braun V. Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12. J Bacteriol. 2004;186:5303-10 pubmed
    ..Comparison of the properties of the FecA, FhuA, FepA, and BtuB transporters indicates that although they have very similar crystal structures, the details of ..
  63. Mademidis A, Koster W. Transport activity of FhuA, FhuC, FhuD, and FhuB derivatives in a system free of polar effects, and stoichiometry of components involved in ferrichrome uptake. Mol Gen Genet. 1998;258:156-65 pubmed
    The Escherichia coli fhu operon, composed of the fhuA, C, D, and B genes, is essential for the utilization of ferric siderophores of the hydroxamate type and for the uptake of the antibiotic albomycin...
  64. Coulton J, Mason P, Cameron D, Carmel G, Jean R, Rode H. Protein fusions of beta-galactosidase to the ferrichrome-iron receptor of Escherichia coli K-12. J Bacteriol. 1986;165:181-92 pubmed
    The fusion-generating phage lambda plac Mu1 was used to produce fusions of lacZ to fhuA, the gene encoding the ferrichrome-iron receptor (FhuA protein) in the outer membrane of Escherichia coli K-12...
  65. Moeck G, Letellier L. Characterization of in vitro interactions between a truncated TonB protein from Escherichia coli and the outer membrane receptors FhuA and FepA. J Bacteriol. 2001;183:2755-64 pubmed
    ..H(6)-'TonB captured both the ferrichrome-iron receptor FhuA and the ferric enterobactin receptor FepA from detergent-solubilized outer membranes in vitro...