fecI

Summary

Gene Symbol: fecI
Description: RNA polymerase sigma-19 factor, fec operon-specific; ECF sigma factor
Alias: ECK4283, JW4253
Species: Escherichia coli str. K-12 substr. MG1655
Products:     fecI

Top Publications

  1. Maeda H, Jishage M, Nomura T, Fujita N, Ishihama A. Two extracytoplasmic function sigma subunits, sigma(E) and sigma(FecI), of Escherichia coli: promoter selectivity and intracellular levels. J Bacteriol. 2000;182:1181-4 pubmed
    ..selectivity of two extracytoplasmic function (ECF) subfamily sigma subunits, sigma(E) (sigma(24)) and sigma(FecI) (sigma(18)), of Escherichia coli RNA polymerase was analyzed by using an in vitro transcription system and various ..
  2. Visca P, Leoni L, Wilson M, Lamont I. Iron transport and regulation, cell signalling and genomics: lessons from Escherichia coli and Pseudomonas. Mol Microbiol. 2002;45:1177-90 pubmed
    ..Two of these proteins, PvdS from Pseudomonas aeruginosa and FecI from Escherichia coli K-12, have been studied extensively...
  3. Enz S, Braun V, Crosa J. Transcription of the region encoding the ferric dicitrate-transport system in Escherichia coli: similarity between promoters for fecA and for extracytoplasmic function sigma factors. Gene. 1995;163:13-8 pubmed
    ..Furthermore, transcription of fecA is dependent on the presence of FecI. The promoter region for the fecA mRNA, a likely site of action for FecI, is not related to the consensus promoter ..
  4. Braun V, Mahren S, Sauter A. Gene regulation by transmembrane signaling. Biometals. 2005;18:507-17 pubmed
    ..receiver in the periplasm and as a signal transmitter across the cytoplasmic membrane into the cytoplasm, where the FecI sigma factor is activated to bind RNA polymerase and specifically initiate transcription of the fecABCDE transport ..
  5. Braun V, Mahren S. Transmembrane transcriptional control (surface signalling) of the Escherichia coli Fec type. FEMS Microbiol Rev. 2005;29:673-84 pubmed
    ..avium, and Ralstonia solanacearum. The genomes of P. putida, P. aeruginosa, Nitrosomonas europaea, Bacteroides thetaiotaomicron and Caulobacter crescentus predict the existence of many more such transcriptional regulatory devices. ..
  6. Hughes K, Mathee K. The anti-sigma factors. Annu Rev Microbiol. 1998;52:231-86 pubmed
    ..The range of cell processes regulated by anti-sigma factors is highly varied and includes bacteriophage phage growth, sporulation, stress response, flagellar biosynthesis, pigment production, ion transport, and virulence. ..
  7. Missiakas D, Raina S. The extracytoplasmic function sigma factors: role and regulation. Mol Microbiol. 1998;28:1059-66 pubmed
    ..Presumably, an on-and-off switch of the anti-sigma activity leads to the release of the sigma factor and thereby to the co-ordinate transcription of the specific regulon it governs. ..
  8. Enz S, Mahren S, Stroeher U, Braun V. Surface signaling in ferric citrate transport gene induction: interaction of the FecA, FecR, and FecI regulatory proteins. J Bacteriol. 2000;182:637-46 pubmed
    ..FecA initiates a signal that is transmitted by FecR across the cytoplasmic membrane into the cytoplasm where FecI, the sigma factor, is activated...
  9. Ochs M, Angerer A, Enz S, Braun V. Surface signaling in transcriptional regulation of the ferric citrate transport system of Escherichia coli: mutational analysis of the alternative sigma factor FecI supports its essential role in fec transport gene transcription. Mol Gen Genet. 1996;250:455-65 pubmed
    ..FecR transmits the signal across the cytoplasmic membrane and activates FecI located in the cytoplasm. FecI belongs to a subgroup of sigma factors that respond to extracytoplasmic stimuli...

More Information

Publications32

  1. Ochs M, Veitinger S, Kim I, Welz D, Angerer A, Braun V. Regulation of citrate-dependent iron transport of Escherichia coli: fecR is required for transcription activation by FecI. Mol Microbiol. 1995;15:119-32 pubmed
    ..Two regulatory genes, fecI and fecR, located upstream of the fecABCDE transport genes, are required for induction...
  2. Braun V, Mahren S, Sauter A. Gene regulation by transmembrane signaling. Biometals. 2006;19:103-13 pubmed
    ..receiver in the periplasm and as a signal transmitter across the cytoplasmic membrane into the cytoplasm, where the FecI sigma factor is activated to bind RNA polymerase and specifically initiate transcription of the fecABCDE transport ..
  3. Enz S, Brand H, Orellana C, Mahren S, Braun V. Sites of interaction between the FecA and FecR signal transduction proteins of ferric citrate transport in Escherichia coli K-12. J Bacteriol. 2003;185:3745-52 pubmed
    ..protein FecA loaded with ferric citrate, transmits the signal across the cytoplasmic membrane, and converts FecI in the cytoplasm to an active sigma factor...
  4. Mahren S, Enz S, Braun V. Functional interaction of region 4 of the extracytoplasmic function sigma factor FecI with the cytoplasmic portion of the FecR transmembrane protein of the Escherichia coli ferric citrate transport system. J Bacteriol. 2002;184:3704-11 pubmed
    ..a signal that is transmitted by FecR across the cytoplasmic membrane into the cytoplasm, where the sigma factor FecI directs the RNA polymerase to the promoter upstream of the fecABCDE genes...
  5. Yoshida M, Kashiwagi K, Shigemasa A, Taniguchi S, Yamamoto K, Makinoshima H, et al. A unifying model for the role of polyamines in bacterial cell growth, the polyamine modulon. J Biol Chem. 2004;279:46008-13 pubmed
    ..In this study we found that the synthesis of FecI and Fis was also stimulated by polyamines at the level of translation...
  6. Brooks B, Buchanan S. Signaling mechanisms for activation of extracytoplasmic function (ECF) sigma factors. Biochim Biophys Acta. 2008;1778:1930-45 pubmed
    ..pathways regulate sigma(E)-mediated expression of periplasmic stress response genes in Escherichia coli and FecI-mediated expression of iron-citrate transport genes in E. coli...
  7. Staudenmaier H, Van Hove B, Yaraghi Z, Braun V. Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli. J Bacteriol. 1989;171:2626-33 pubmed
    ..The close homology between the proteins of the two iron transport systems and of the vitamin B12 transport system indicates a common evolution for all three systems. ..
  8. Mahren S, Braun V. The FecI extracytoplasmic-function sigma factor of Escherichia coli interacts with the beta' subunit of RNA polymerase. J Bacteriol. 2003;185:1796-802 pubmed
    ..citrate transport system of Escherichia coli K-12 is mediated by the extracytoplasmic-function (ECF) sigma factor FecI, which is activated by ferric citrate in the growth medium...
  9. Igarashi K, Kashiwagi K. Polyamine Modulon in Escherichia coli: genes involved in the stimulation of cell growth by polyamines. J Biochem. 2006;139:11-6 pubmed
    ..adenylate cyclase (Cya), RNA polymerase sigma(38) subunit (RpoS), transcription factor of iron transport operon (FecI), and transcription factor of growth-related genes including rRNA and some kinds of tRNA synthesis (Fis) was ..
  10. Angerer A, Enz S, Ochs M, Braun V. Transcriptional regulation of ferric citrate transport in Escherichia coli K-12. Fecl belongs to a new subfamily of sigma 70-type factors that respond to extracytoplasmic stimuli. Mol Microbiol. 1995;18:163-74 pubmed
    ..Presumably, a conformational change is transmitted in a TonB-dependent manner to the FecR protein. FecR activates FecI, and FecI activates transcription of the fecABCDE transport genes...
  11. Braun V, Mahren S, Ogierman M. Regulation of the FecI-type ECF sigma factor by transmembrane signalling. Curr Opin Microbiol. 2003;6:173-80 pubmed
    ..Three specific proteins are involved: FecA in the outer membrane, FecR in the cytoplasmic membrane, and FecI in the cytoplasm...
  12. Braun V. Surface signaling: novel transcription initiation mechanism starting from the cell surface. Arch Microbiol. 1997;167:325-31 pubmed
    ..The signal is then transduced across the cytoplasmic membrane by the FecR protein, which in turn activates the FecI sigma-factor that directs the RNA polymerase core-enzyme to the fec transport gene promoter...
  13. Mahren S, Schnell H, Braun V. Occurrence and regulation of the ferric citrate transport system in Escherichia coli B, Klebsiella pneumoniae, Enterobacter aerogenes, and Photorhabdus luminescens. Arch Microbiol. 2005;184:175-86 pubmed
    ..The FecI sigma factor is only active in the presence of FecR, which transfers the signal across the cytoplasmic membrane...
  14. Van Hove B, Staudenmaier H, Braun V. Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12. J Bacteriol. 1990;172:6749-58 pubmed
    ..The five transport genes fecABCDE form an operon and are transcribed from fecA to fecE. Two genes, termed fecI and fecR, that mediate induction by iron(III) dicitrate have been identified upstream of fecA...
  15. Arechaga I, Miroux B, Runswick M, Walker J. Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript. FEBS Lett. 2003;547:97-100 pubmed
    ..Green fluorescent protein fusions with N- and C-ends of uncB helped to stabilize the mRNA and to obtain high yields of protein. ..
  16. Veitinger S, Braun V. Localization of the entire fec region at 97.3 minutes on the Escherichia coli K-12 chromosome. J Bacteriol. 1992;174:3838-9 pubmed
  17. Pressler U, Staudenmaier H, Zimmermann L, Braun V. Genetics of the iron dicitrate transport system of Escherichia coli. J Bacteriol. 1988;170:2716-24 pubmed
    ..In Mu d1 insertion mutants upstream of fecA, the fec genes were not inducible by iron limitation and citrate, indicating a regulatory region, termed fecI, which controls fec gene expression.
  18. Harle C, Kim I, Angerer A, Braun V. Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. EMBO J. 1995;14:1430-8 pubmed
    ..the outer membrane, while the regulatory protein FecR transmits the signal across the cytoplasmic membrane to FecI in the cytoplasm...
  19. Stiefel A, Mahren S, Ochs M, Schindler P, Enz S, Braun V. Control of the ferric citrate transport system of Escherichia coli: mutations in region 2.1 of the FecI extracytoplasmic-function sigma factor suppress mutations in the FecR transmembrane regulatory protein. J Bacteriol. 2001;183:162-70 pubmed
    ..by FecA across the outer membrane and by FecR across the cytoplasmic membrane into the cytoplasm, where the FecI extracytoplasmic-function (ECF) sigma factor becomes active...
  20. Wagegg W, Braun V. Ferric citrate transport in Escherichia coli requires outer membrane receptor protein fecA. J Bacteriol. 1981;145:156-63 pubmed
    ..E. coli W83-24 and Salmonella typhimurium, which are devoid of a citrate-dependent iron transport system, lacked the FecA protein. It is proposed that the FecA protein participates in the transport of ferric citrate. ..
  21. Lonetto M, Brown K, Rudd K, Buttner M. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci U S A. 1994;91:7573-7 pubmed
    ..coli FecI, and Bacillus subtilis SigX, a protein of unknown function...
  22. Staron A, Sofia H, Dietrich S, Ulrich L, Liesegang H, Mascher T. The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family. Mol Microbiol. 2009;74:557-81 pubmed publisher
    ..This classification, together with the web tool ECFfinder and the information stored in the Microbial Signal Transduction (MiST) database, provides a comprehensive resource for the analysis of ECF sigma factor-dependent gene regulation. ..
  23. Enz S, Mahren S, Menzel C, Braun V. Analysis of the ferric citrate transport gene promoter of Escherichia coli. J Bacteriol. 2003;185:2387-91 pubmed
    b>FecI, an extracytoplasmic-function sigma factor, is required for initiation of transcription of the ferric citrate transport genes...