fabD

Summary

Gene Symbol: fabD
Description: malonyl-CoA-[acyl-carrier-protein] transacylase
Alias: ECK1078, JW1078, tfpA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Serre L, Verbree E, Dauter Z, Stuitje A, Derewenda Z. The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component. J Biol Chem. 1995;270:12961-4 pubmed
    ..Two other residues, Arg-117 and Glu-11, are also located in the active site, although their function is not clear. ..
  2. Xu H, Real L, Bailey M. An array of Escherichia coli clones over-expressing essential proteins: a new strategy of identifying cellular targets of potent antibacterial compounds. Biochem Biophys Res Commun. 2006;349:1250-7 pubmed
    ..Target identification via target protein over-expression was demonstrated using both mixed clone and individual clone assay formats. ..
  3. Oefner C, Schulz H, D Arcy A, Dale G. Mapping the active site of Escherichia coli malonyl-CoA-acyl carrier protein transacylase (FabD) by protein crystallography. Acta Crystallogr D Biol Crystallogr. 2006;62:613-8 pubmed
    Malonyl-CoA-acyl carrier protein transacylase (FabD; EC 2.3.1...
  4. Zhang Y, Marrakchi H, White S, Rock C. The application of computational methods to explore the diversity and structure of bacterial fatty acid synthase. J Lipid Res. 2003;44:1-10 pubmed
  5. Zhang Y, Cronan J. Transcriptional analysis of essential genes of the Escherichia coli fatty acid biosynthesis gene cluster by functional replacement with the analogous Salmonella typhimurium gene cluster. J Bacteriol. 1998;180:3295-303 pubmed
    ..encoding several key fatty acid biosynthetic enzymes (called the fab cluster) are clustered in the order plsX-fabH-fabD-fabG-acpP-fabF at min 24 of the Escherichia coli chromosome...
  6. Zhang Y, Cronan J. Polar allele duplication for transcriptional analysis of consecutive essential genes: application to a cluster of Escherichia coli fatty acid biosynthetic genes. J Bacteriol. 1996;178:3614-20 pubmed
    ..By use of this method (called polar allele duplication), we demonstrate that the promoter located immediately upstream of the coding sequence is sufficient for synthesis of this very abundant protein. ..
  7. Verwoert I, Verhagen E, van der Linden K, Verbree E, Nijkamp H, Stuitje A. Molecular characterization of an Escherichia coli mutant with a temperature-sensitive malonyl coenzyme A-acyl carrier protein transacylase. FEBS Lett. 1994;348:311-6 pubmed
    ..coli strain LA2-89, carrying the fabD89 allele, was shown to result from the presence of an amber mutation in the fabD gene, at codon position 257, in combination with the supE44 genotype of this strain...
  8. Verwoert I, Verbree E, van der Linden K, Nijkamp H, Stuitje A. Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase. J Bacteriol. 1992;174:2851-7 pubmed
    The Escherichia coli fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase (MCT) was cloned by complementation of a thermosensitive E. coli fabD mutant (fabD89). Expression of the fabD gene in an appropriate E...
  9. Bouquin N, Tempete M, Holland I, Seror S. Resistance to trifluoroperazine, a calmodulin inhibitor, maps to the fabD locus in Escherichia coli. Mol Gen Genet. 1995;246:628-37 pubmed
    ..Subsequent subcloning, complementation analysis, marker rescue mapping and sequencing, identified tfpA as fabD, encoding the 35 kDa, malonyl-coenzyme A transacylase (MCT) enzyme, required for the initial step in the elongation ..

More Information

Publications18

  1. Podkovyrov S, Larson T. Lipid biosynthetic genes and a ribosomal protein gene are cotranscribed. FEBS Lett. 1995;368:429-31 pubmed
    ..plsX gene encoding a protein involved in membrane lipid synthesis and several fatty acid biosynthetic genes (fabH, fabD and fabG) are cotranscribed...
  2. Joshi V, Wakil S. Studies on the mechanism of fatty acid synthesis. XXVI. Purification and properties of malonyl-coenzyme A--acyl carrier protein transacylase of Escherichia coli. Arch Biochem Biophys. 1971;143:493-505 pubmed
  3. Ruch F, Vagelos P. Characterization of a malonyl-enzyme intermediate and identification of the malonyl binding site in malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli. J Biol Chem. 1973;248:8095-106 pubmed
  4. Garwin J, Klages A, Cronan J. Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli. J Biol Chem. 1980;255:11949-56 pubmed
    ..fabF mutants of E. coli lack synthase II. The fabF locus was mapped at min 24.5 of the E. coli genetic map and the clockwise map order was found to be pyrC, fabD, fabF, purB.
  5. Podkovyrov S, Larson T. Identification of promoter and stringent regulation of transcription of the fabH, fabD and fabG genes encoding fatty acid biosynthetic enzymes of Escherichia coli. Nucleic Acids Res. 1996;24:1747-52 pubmed
    ..By using primer extension analysis we identified the fabH promoter responsible for transcription of the fabH, fabD and fabG genes encoding fatty acid biosynthetic enzymes...
  6. Ruch F, Vagelos P. The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier protein transacylase. J Biol Chem. 1973;248:8086-94 pubmed
  7. White S, Zheng J, Zhang Y, Rock -. The structural biology of type II fatty acid biosynthesis. Annu Rev Biochem. 2005;74:791-831 pubmed
    ..These structures are also a valuable resource to guide antibacterial drug discovery. ..
  8. Rawlings M, Cronan J. The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes. J Biol Chem. 1992;267:5751-4 pubmed
    ..The ACP gene (called acpP) was located on the genetic map between fabF and fabD which encode two fatty acid biosynthetic enzymes, 3-ketoacyl-ACP synthase II and malonyl CoA-ACP transacylase, ..
  9. Magnuson K, Oh W, Larson T, Cronan J. Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli. FEBS Lett. 1992;299:262-6 pubmed
    ..Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids. ..