fabA

Summary

Gene Symbol: fabA
Description: beta-hydroxydecanoyl thioester dehydrase
Alias: ECK0945, JW0937
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases
    Shane C Dillon
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Bioinformatics 5:109. 2004
  2. ncbi Roles of the FabA and FabZ beta-hydroxyacyl-acyl carrier protein dehydratases in Escherichia coli fatty acid biosynthesis
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:27795-801. 1996
  3. ncbi Structure of a dehydratase-isomerase from the bacterial pathway for biosynthesis of unsaturated fatty acids: two catalytic activities in one active site
    M Leesong
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Structure 4:253-64. 1996
  4. pmc Crystal structures of acyl carrier protein in complex with two catalytic partners show a dynamic role in cellular metabolism
    Sean A Newmister
    Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109 USA
    Chembiochem 15:1079-81. 2014
  5. ncbi Escherichia coli beta-hydroxydecanoyl thioester dehydrase reacts with native C10 acyl-acyl-carrier proteins of plant and bacterial origin
    D J Guerra
    Institute of Biological Chemistry, Washington State University, Pullman 99164 6340
    Arch Biochem Biophys 280:336-45. 1990
  6. pmc Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli
    R Sugai
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 183:5523-8. 2001
  7. ncbi A new mechanism of transcriptional regulation: release of an activator triggered by small molecule binding
    M F Henry
    Department of Microbiology, University of Illinois, Urbana 61801
    Cell 70:671-9. 1992
  8. pmc Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters
    Youjun Feng
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 80:195-218. 2011
  9. doi Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation
    Lian Hua Luo
    Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580 185, Jeonbuk, South Korea
    Biotechnol Lett 31:1867-71. 2009
  10. pmc Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB
    Youjun Feng
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 284:29526-35. 2009

Research Grants

Scientific Experts

  • C O Rock
  • STEPHEN W contact WHITE
  • Lakshmi S Pidugu
  • Shane C Dillon
  • Youjun Feng
  • Yong Mei Zhang
  • Sean A Newmister
  • John E Cronan
  • Lian Hua Luo
  • Yeon Seok Kim
  • Maria C Mansilla
  • Rie Sugai
  • Hedia Marrakchi
  • David H Sherman
  • R Sugai
  • L McCue
  • Pil Soo Seo
  • Sun Yeon Heo
  • Dae Hyuk Kim
  • Chul Ho Kim
  • Jeong Woo Seo
  • Ji Hyun Park
  • Han Na Hong
  • Jiho Min
  • Man Bock Gu
  • Kyeong Seo Park
  • Hajime Tokuda
  • Larisa E Cybulski
  • J E Cronan
  • Hisayo Shimizu
  • Daniela Albanesi
  • Ken ichi Nishiyama
  • Diego de Mendoza
  • R J Heath
  • M Leesong
  • V Derbyshire
  • M P Ryan
  • C Carmack
  • K Nishiyama
  • H Tokuda
  • H Shimizu
  • W Thompson
  • J S Liu
  • C E Lawrence
  • K Saito
  • J M Schwab
  • B S Henderson
  • J L Smith
  • C C DiRusso
  • M F Henry
  • J R Gillig
  • K Magnuson
  • M Ohkuma
  • A Hamajima
  • A Kawaguchi
  • T H Teeri
  • S Ohmori
  • I Murakoshi
  • S Jackowski
  • T L Heimert
  • A K Metzger
  • W D Nunn
  • A Sharma
  • D J Guerra
  • W B Li
  • K Giffin
  • J A Browse
  • M Narasimhan
  • R Coleman
  • D Clark
  • D de Mendoza

Detail Information

Publications31

  1. pmc The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases
    Shane C Dillon
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Bioinformatics 5:109. 2004
    The Hotdog fold was initially identified in the structure of Escherichia coli FabA and subsequently in 4-hydroxybenzoyl-CoA thioesterase from Pseudomonas sp. strain CBS...
  2. ncbi Roles of the FabA and FabZ beta-hydroxyacyl-acyl carrier protein dehydratases in Escherichia coli fatty acid biosynthesis
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:27795-801. 1996
    There are two genes, fabA and fabZ, encoding beta-hydroxyacyl-acyl carrier protein (ACP) dehydratases that function in the dissociated, type II fatty acid synthase system of Escherichia coli...
  3. ncbi Structure of a dehydratase-isomerase from the bacterial pathway for biosynthesis of unsaturated fatty acids: two catalytic activities in one active site
    M Leesong
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Structure 4:253-64. 1996
    ..The aim of this work is to elucidate mechanisms for the two enzymatic reactions, which occur in an unusual bifunctional active site, and to understand the specificity of the enzyme for substrates with 10-carbon fatty acyl chains...
  4. pmc Crystal structures of acyl carrier protein in complex with two catalytic partners show a dynamic role in cellular metabolism
    Sean A Newmister
    Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109 USA
    Chembiochem 15:1079-81. 2014
    ..These studies highlight key interactions of transient ACP-partner complexes and define the dynamic movements of ACP that facilitate substrate delivery and trigger complex dissociation. ..
  5. ncbi Escherichia coli beta-hydroxydecanoyl thioester dehydrase reacts with native C10 acyl-acyl-carrier proteins of plant and bacterial origin
    D J Guerra
    Institute of Biological Chemistry, Washington State University, Pullman 99164 6340
    Arch Biochem Biophys 280:336-45. 1990
    ..J. Guerra, J. B. Ohlrogge, and M. Frentzen, 1986, Plant Physiol. 82, 448-453)...
  6. pmc Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli
    R Sugai
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 183:5523-8. 2001
    ..Taken together, these results suggest that an increase in membrane fluidity due to the increase in unsaturated fatty acids compensates for the absence of the SecG function, especially at low temperature...
  7. ncbi A new mechanism of transcriptional regulation: release of an activator triggered by small molecule binding
    M F Henry
    Department of Microbiology, University of Illinois, Urbana 61801
    Cell 70:671-9. 1992
    The FadR protein of E. coli activates transcription of the fabA gene, a key enzyme of fatty acid synthesis...
  8. pmc Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters
    Youjun Feng
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 80:195-218. 2011
    ..FabR represses expression of the two genes, fabA and fabB, required for unsaturated fatty acid synthesis and has been reported to require the presence of an ..
  9. doi Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation
    Lian Hua Luo
    Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580 185, Jeonbuk, South Korea
    Biotechnol Lett 31:1867-71. 2009
    ..in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding beta-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli...
  10. pmc Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB
    Youjun Feng
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 284:29526-35. 2009
    ..The key players are the fabA and fabB genes...
  11. pmc Analysis of proteins with the 'hot dog' fold: prediction of function and identification of catalytic residues of hypothetical proteins
    Lakshmi S Pidugu
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    BMC Struct Biol 9:37. 2009
    ....
  12. ncbi Gene expression analysis and classification of mode of toxicity of polycyclic aromatic hydrocarbons (PAHs) in Escherichia coli
    Yeon Seok Kim
    College of Life Sciences and Biotechnology, Korea University, Anam Dong, Sungbuk Gu, Seoul 136 701, South Korea
    Chemosphere 66:1243-8. 2007
    ..In this study, therefore, the expression of recA, katG, fabA and grpE genes was used as a representative for DNA, oxidative, membrane and protein damage, respectively, after E...
  13. ncbi The structural biology of type II fatty acid biosynthesis
    Stephen W White
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Annu Rev Biochem 74:791-831. 2005
    ..These structures are also a valuable resource to guide antibacterial drug discovery...
  14. pmc Control of membrane lipid fluidity by molecular thermosensors
    Maria C Mansilla
    Instituto de BiologĂ­a Molecular y Celular de Rosario IBR and Departamento de MicrobiologĂ­a, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina
    J Bacteriol 186:6681-8. 2004
  15. pmc Overexpression of gnsA, a multicopy suppressor of the secG null mutation, increases acidic phospholipid contents by inhibiting phosphatidylethanolamine synthesis at low temperatures
    Rie Sugai
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 186:5968-71. 2004
    ..GnsA overproduction at 20 degrees C specifically inhibited phosphatidylethanolamine synthesis and therefore caused the increase in the proportion of acidic phospholipids...
  16. ncbi Escherichia coli transcription factor that both activates fatty acid synthesis and represses fatty acid degradation
    M F Henry
    Department of Microbiology, University of Illinois, Urbana 61801
    J Mol Biol 222:843-9. 1991
    ..We report that the FadR protein also functions as a positive transcriptional activator of the fabA gene, which encodes the enzyme introducing the double bond of the unsaturated fatty acids of E. coli.
  17. ncbi The application of computational methods to explore the diversity and structure of bacterial fatty acid synthase
    Yong Mei Zhang
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Lipid Res 44:1-10. 2003
    ....
  18. ncbi The FabR (YijC) transcription factor regulates unsaturated fatty acid biosynthesis in Escherichia coli
    Yong Mei Zhang
    Department of Infectious Diseases, Protein Science Division, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 277:15558-65. 2002
    ..fatty acid biosynthesis is a vital facet of Escherichia coli physiology and requires the expression of two genes, fabA and fabB, in the type II fatty acid synthase system...
  19. ncbi Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase
    J E Cronan
    Department of Microbiology, University of Illinios, Urbana 61801
    J Biol Chem 263:4641-6. 1988
    The nucleotide sequence of the fabA gene encoding beta-hydroxydecanoyl thioester dehydrase, a key enzyme of the unsaturated fatty acid synthesis pathway of Escherichia coli, has been determined by the dideoxynucleotide sequencing ..
  20. pmc Phylogenetic footprinting of transcription factor binding sites in proteobacterial genomes
    L McCue
    The Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, NY 12201, USA
    Nucleic Acids Res 29:774-82. 2001
    ..was proven by affinity purification of a putative transcription factor (YijC) bound to such a site upstream of the fabA gene. Predicted regulatory sites for 2097 E.coli genes are available at http://www.wadsworth.org/resnres/bioinfo/.
  21. ncbi Crystallization and preliminary X-ray analysis of beta-hydroxydecanoyl thiol ester dehydrase from Escherichia coli
    A Sharma
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
    J Biol Chem 265:5110-2. 1990
    ..The orthorhombic crystals belong to space group P2(1)2(1)2(1), with cell constants of a = 68.4 A, b = 87.3 A, and c = 60.3 A. Monoclinic crystals are of space group C2, with a = 131.9 A, b = 71.5 A, c = 92.5 A, and beta = 103.5 degrees...
  22. pmc Increased unsaturated fatty acid production associated with a suppressor of the fabA6(Ts) mutation in Escherichia coli
    C O Rock
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Bacteriol 178:5382-7. 1996
    ..Subcloning and physical mapping localized the new gene (called sfa for suppressor of fabA) at 1,070 kb on the E. coli chromosome...
  23. ncbi Regulation of transcription of genes required for fatty acid transport and unsaturated fatty acid biosynthesis in Escherichia coli by FadR
    C C DiRusso
    Department of Biochemistry, University of Tennessee, Memphis 38163
    Mol Microbiol 7:311-22. 1993
    ..In the present work we investigate FadR interaction with the fabA and fadL promoters...
  24. pmc Regulation of fatty acid biosynthesis in Escherichia coli
    K Magnuson
    Department of Microbiology, University of Illinois, Urbana 61801
    Microbiol Rev 57:522-42. 1993
    ..Pursuing such questions should reveal additional regulatory mechanisms of fatty acid synthesis and, hopefully, the role of fatty acid synthesis and other cellular processes in the global control of cellular growth...
  25. ncbi Expression of the Escherichia coli fabA gene encoding beta-hydroxydecanoyl thioester dehydrase and transport to chloroplasts in transgenic tobacco
    K Saito
    Faculty of Pharmaceutical Sciences, Laboratory of Molecular Biology and Biotechnology in Research Center of Medicinal Resources, Chiba University, Japan
    Transgenic Res 4:60-9. 1995
    The fabA gene of Escherichia coli encodes beta-hydroxydecanoyl thioester dehydrase (HDDase), a pivotal enzyme in the biosynthesis of the unsaturated fatty acid cis-vaccenic acid, through the anaerobic pathway...
  26. pmc Role for fadR in unsaturated fatty acid biosynthesis in Escherichia coli
    W D Nunn
    J Bacteriol 154:554-60. 1983
    ..comparable to those of fadR+ strains is phenotypically asymptomatic unless the fadR strain also carries a lesion in fabA, the structural gene for beta-hydroxydecanoyl-thioester dehydrase...

Research Grants2

  1. STRUCTURAL STUDIES ON RIBOSOMAL COMPONENTS
    Stephen White; Fiscal Year: 2002
    ..Future work will improve this image to accurately orient the IF3 molecule, and additional factors will be added to build up the complete initiation complex. ..
  2. Development of DHPS as a Bioterrorism Therapeutic Target
    Stephen White; Fiscal Year: 2005
    ..The institutions have complementary programs in infectious diseases with a strong emphasis on studying bioterrorism. ..