Gene Symbol: ECs5153
Description: adenylosuccinate synthetase
Species: Escherichia coli O157:H7 str. Sakai
Poland B, Fromm H, Honzatko R. Crystal structures of adenylosuccinate synthetase from Escherichia coli complexed with GDP, IMP hadacidin, NO3-, and Mg2+. J Mol Biol. 1996;264:1013-27 pubmed
..A mechanism is proposed for the two-step reaction governed by the synthetase, in which His41 and Asp13 are essential catalytic side-chains. ..
Liu F, Dong Q, Fromm H. Site-directed mutagenesis of the phosphate-binding consensus sequence in Escherichia coli adenylosuccinate synthetase. J Biol Chem. 1992;267:2388-92 pubmed
..These findings suggest that the glycine-rich sequence in adenylosuccinate synthetase functions differently relative to those in other GTP binding proteins or enzymes. ..
Poland B, Lee S, Subramanian M, Siehl D, Anderson R, Fromm H, et al
. Refined crystal structure of adenylosuccinate synthetase from Escherichia coli complexed with hydantocidin 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin. Biochemistry. 1996;35:15753-9 pubmed
..Hydantocidin 5'-phosphate, GDP, HPO4(2-), and Mg2+ may represent a set of synergistic inhibitors even more effective than the combination of IMP, GDP, NO3-, and Mg2+. ..
Choe J, Poland B, Fromm H, Honzatko R. Mechanistic implications from crystalline complexes of wild-type and mutant adenylosuccinate synthetases from Escherichia coli. Biochemistry. 1999;38:6953-61 pubmed
Hou Z, Cashel M, Fromm H, Honzatko R. Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase. J Biol Chem. 1999;274:17505-10 pubmed
..Conceivably, synthetase activity in vivo during the stringent response may be sensitive to the relative concentrations of several effectors, which together exercise precise control over the de novo synthesis of AMP. ..
Silva M, Poland B, Hoffman C, Fromm H, Honzatko R. Refined crystal structures of unligated adenylosuccinate synthetase from Escherichia coli. J Mol Biol. 1995;254:431-46 pubmed
..The structural elements of the p21 ras and G alpha proteins responsible for binding Mg2+ are either absent from the synthetase or unavailable for the coordination of metal cations. ..
Hou Z, Wang W, Fromm H, Honzatko R. IMP Alone Organizes the Active Site of Adenylosuccinate Synthetase from Escherichia coli. J Biol Chem. 2002;277:5970-6 pubmed
Dong Q, Liu F, Myers A, Fromm H. Evidence for an arginine residue at the substrate binding site of Escherichia coli adenylosuccinate synthetase as studied by chemical modification and site-directed mutagenesis. J Biol Chem. 1991;266:12228-33 pubmed
..In addition, kcat of the R147L mutant decreased by a factor of 1.3 x 10(4). On the bases of these observations, it is suggested that Arg147 is critical for enzyme catalysis...
Dong Q, Fromm H. Chemical modification of adenylosuccinate synthetase from Escherichia coli by pyridoxal 5'-phosphate. Identification of an active site lysyl residue. J Biol Chem. 1990;265:6235-40 pubmed
..Replacing the Lys140 with Ile140 by site-directed mutagenesis resulted in total loss of the activity. These results suggest that Lys140 may play an important role in enzymatic activity. ..