| ECs4704Gene Symbol: ECs4704 Description: branched-chain amino acid aminotransferase Species: Escherichia coli O157:H7 str. Sakai Okada K, Hirotsu K, Hayashi H, Kagamiyama H. Structures of Escherichia coli branched-chain amino acid aminotransferase and its complexes with 4-methylvalerate and 2-methylleucine: induced fit and substrate recognition of the enzyme. Biochemistry. 2001;40:7453-63 pubmed Pagel J, Winkelman J, Adams C, Hatfield G. DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli. J Mol Biol. 1992;224:919-35 pubmed Inoue K, Kuramitsu S, Aki K, Watanabe Y, Takagi T, Nishigai M, et al. Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties. J Biochem. 1988;104:777-84 pubmed ..Each subunit bound approximately 1 mol of pyridoxal 5'-phosphate. A lysyl residue, which forms a Schiff base with the aldehyde group of the pyridoxal 5'-phosphate, was identified in the primary structure of the enzyme. .. Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H. Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence. J Biochem. 1985;97:993-9 pubmed ..C., et al. (1979) J. Bacteriol. 139, 339-345). From the deduced amino acid sequence, the secondary structure was predicted... Lawther R, Nichols B, Zurawski G, Hatfield G. The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12. Nucleic Acids Res. 1979;7:2289-301 pubmed ..These results are consistent with the suggestion that the ilvEDA operon is regulated by an attenuator mechanism and that the attenuator region lies before the ilvG gene. .. Lawther R, Wek R, Lopes J, Pereira R, Taillon B, Hatfield G. The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 1987;15:2137-55 pubmed ..This new information contributes to a more complete understanding of the overall control of the biosynthesis of isoleucine and valine... Lawther R, Calhoun D, Adams C, Hauser C, Gray J, Hatfield G. Molecular basis of valine resistance in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1981;78:922-5 pubmed Parekh B, Sheridan S, Hatfield G. Effects of integration host factor and DNA supercoiling on transcription from the ilvPG promoter of Escherichia coli. J Biol Chem. 1996;271:20258-64 pubmed ..Therefore, IHF binding does not activate transcription simply by increasing the local negative supercoiling of the DNA helix in the downstream promoter region or by differential binding to relaxed and supercoiled DNA templates. .. Okada K, Hirotsu K, Sato M, Hayashi H, Kagamiyama H. Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5 A resolution. J Biochem. 1997;121:637-41 pubmed ..Although the main chain conformation of the active site is homologous to that of D-amino acid aminotransferase, many of the active site residues are different between them. ..
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