Gene Symbol: ECs4374
Description: arsenical pump membrane protein
Species: Escherichia coli O157:H7 str. Sakai
Wu J, Tisa L, Rosen B. Membrane topology of the ArsB protein, the membrane subunit of an anion-translocating ATPase. J Biol Chem. 1992;267:12570-6 pubmed
..The location of a periplasmic loop was determined from V8 protease digestion of an ArsA-ArsB chimera. From analysis of data from 26 fusions, a topological model of the ArsB protein with 12 membrane-spanning regions is proposed. ..
Tisa L, Rosen B. Molecular characterization of an anion pump. The ArsB protein is the membrane anchor for the ArsA protein. J Biol Chem. 1990;265:190-4 pubmed
Rosen B. The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase. Res Microbiol. 1990;141:336-41 pubmed
..This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. ..
San Francisco M, Tisa L, Rosen B. Identification of the membrane component of the anion pump encoded by the arsenical resistance operon of R-factor R773. Mol Microbiol. 1989;3:15-21 pubmed
..In addition to the previously identified ArsA and ArsC proteins, the cells synthesized an inner membrane protein with an apparent mass of 36 kD identified as the ArsB protein. ..
Diorio C, Cai J, Marmor J, Shinder R, DuBow M. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria. J Bacteriol. 1995;177:2050-6 pubmed
Chen C, Misra T, Silver S, Rosen B. Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon. J Biol Chem. 1986;261:15030-8 pubmed
..A model is proposed in which these gene products form an anion translocating ATPase for extrusion of arsenite and arsenate from resistant cells. ..
Bruhn D, Li J, Silver S, Roberto F, Rosen B. The arsenical resistance operon of IncN plasmid R46. FEMS Microbiol Lett. 1996;139:149-53 pubmed
..The R46 system contains only the second known versions of arsA and arsD, after those of plasmid R773. Western blot analysis identified the R46 proteins using antibodies against R773 ArsA, ArsD and ArsR. ..