Gene Symbol: ECs3395
Description: scaffold protein
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Muhlenhoff U, Lill R. Biogenesis of iron-sulfur proteins in eukaryotes: a novel task of mitochondria that is inherited from bacteria. Biochim Biophys Acta. 2000;1459:370-82 pubmed
    ..This review summarizes recent developments in our understanding of the biogenesis of iron-sulfur proteins both within bacteria and eukaryotes. ..
  2. Silberg J, Hoff K, Tapley T, Vickery L. The Fe/S assembly protein IscU behaves as a substrate for the molecular chaperone Hsc66 from Escherichia coli. J Biol Chem. 2001;276:1696-700 pubmed
    ..These results support a role for IscU as a substrate for Hsc66 and suggest a specialized function for Hsc66 in the assembly, stabilization, or transfer of Fe/S clusters formed on IscU. ..
  3. Urbina H, Silberg J, Hoff K, Vickery L. Transfer of sulfur from IscS to IscU during Fe/S cluster assembly. J Biol Chem. 2001;276:44521-6 pubmed publisher
    ..The findings with IscSDelta376-413 provide additional support for a mechanism of sulfur transfer involving a direct interaction between IscS and IscU and suggest that the C-terminal region of IscS may be important for binding IscU...
  4. Tokumoto U, Nomura S, Minami Y, Mihara H, Kato S, Kurihara T, et al. Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. J Biochem. 2002;131:713-9 pubmed
    ..Thus, the sequential association and dissociation among the IscS, IscU, IscA, HscB, HscA, Fdx, and ORF3 proteins may be a critical process in the assembly of Fe-S clusters...
  5. Hoff K, Cupp Vickery J, Vickery L. Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system. J Biol Chem. 2003;278:37582-9 pubmed publisher
    ..The results are discussed in the context of a structural model based on the crystallographic structure of the DnaK peptide-binding domain...
  6. Takahashi Y, Nakamura M. Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J Biochem. 1999;126:917-26 pubmed
    ..We propose a model in which the fdx gene product functions as an intermediate site for Fe-S cluster assembly. ..
  7. Hoff K, Silberg J, Vickery L. Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli. Proc Natl Acad Sci U S A. 2000;97:7790-5 pubmed
    ..These results establish a direct and specific role for the Hsc66/Hsc20 chaperone system in functioning with isc gene components for the assembly of iron-sulfur cluster proteins. ..
  8. Tokumoto U, Takahashi Y. Genetic analysis of the isc operon in Escherichia coli involved in the biogenesis of cellular iron-sulfur proteins. J Biochem. 2001;130:63-71 pubmed
    ..The results presented here indicate crucial roles for IscU, HscB, HscA, and Fdx as central components of the assembler machinery and also provide evidence for interactions among them...
  9. Schwartz C, Giel J, Patschkowski T, Luther C, Ruzicka F, Beinert H, et al. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci U S A. 2001;98:14895-900 pubmed publisher

More Information


  1. Nuth M, Yoon T, Cowan J. Iron-sulfur cluster biosynthesis: characterization of iron nucleation sites for assembly of the [2Fe-2S]2+ cluster core in IscU proteins. J Am Chem Soc. 2002;124:8774-5 pubmed
    ..The latter observation reflects the low binding affinity of persulfido IscU for iron ion...
  2. Frazzon J, Fick J, Dean D. Biosynthesis of iron-sulphur clusters is a complex and highly conserved process. Biochem Soc Trans. 2002;30:680-5 pubmed
  3. Nakamura M, Saeki K, Takahashi Y. Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hs cA-fdx-ORF3 gene cluster. J Biochem. 1999;126:10-8 pubmed
    ..These results indicate that the proteins encoded by the gene cluster are involved in the assembly of the Fe-S clusters in a wide variety of Fe-S proteins. ..
  4. Kato S, Mihara H, Kurihara T, Takahashi Y, Tokumoto U, Yoshimura T, et al. Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: implications for the mechanism of iron-sulfur cluster assembly. Proc Natl Acad Sci U S A. 2002;99:5948-52 pubmed publisher
  5. Smith A, Agar J, Johnson K, Frazzon J, Amster I, Dean D, et al. Sulfur transfer from IscS to IscU: the first step in iron-sulfur cluster biosynthesis. J Am Chem Soc. 2001;123:11103-4 pubmed
  6. Kurihara T, Mihara H, Kato S, Yoshimura T, Esaki N. Assembly of iron-sulfur clusters mediated by cysteine desulfurases, IscS, CsdB and CSD, from Escherichia coli. Biochim Biophys Acta. 2003;1647:303-9 pubmed
  7. Hoff K, Ta D, Tapley T, Silberg J, Vickery L. Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU. J Biol Chem. 2002;277:27353-9 pubmed publisher
  8. Zheng L, Cash V, Flint D, Dean D. Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii. J Biol Chem. 1998;273:13264-72 pubmed
    ..These results show that the cysE-like gene located upstream from iscS is not essential for cell growth and that the cysE-like gene and the iscSUA-hscBA-fdx genes are contained within separate transcription units. ..