Gene Symbol: ECs0482
Description: protoheme IX farnesyltransferase
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Saiki K, Mogi T, Anraku Y. Heme O biosynthesis in Escherichia coli: the cyoE gene in the cytochrome bo operon encodes a protoheme IX farnesyltransferase. Biochem Biophys Res Commun. 1992;189:1491-7 pubmed
    ..Since the CyoE protein contains the putative allylic polyprenyldiphosphate binding domain, we concluded that the cyoE gene encodes a novel enzyme, protoheme IX farnesyltransferase, essential for heme O biosynthesis. ..
  2. Chepuri V, Lemieux L, Au D, Gennis R. The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases. J Biol Chem. 1990;265:11185-92 pubmed
    ..coli oxidase and the aa3-type cytochrome c oxidases must have very similar structures, at least in the vicinity of the catalytic centers, and they are very likely to have similar mechanisms for bioenergetic coupling (proton pumping). ..
  3. Saiki K, Mogi T, Ogura K, Anraku Y. In vitro heme O synthesis by the cyoE gene product from Escherichia coli. J Biol Chem. 1993;268:26041-4 pubmed
    ..coli encodes not only subunits of the cytochrome bo complex but also heme O synthase that is specifically required for functional expression of the bo-type quinol oxidase. Heme O seems to be an intermediate in heme A biosynthesis. ..
  4. Saiki K, Mogi T, Hori H, Tsubaki M, Anraku Y. Identification of the functional domains in heme O synthase. Site-directed mutagenesis studies on the cyoE gene of the cytochrome bo operon in Escherichia coli. J Biol Chem. 1993;268:26927-34 pubmed
    ..Based on the above observations, we suggest that the conserved amino acid residues present in the cytoplasmic loops II/III and IV/V are part of the catalytic center of heme O synthase. ..
  5. Chepuri V, Gennis R. The use of gene fusions to determine the topology of all of the subunits of the cytochrome o terminal oxidase complex of Escherichia coli. J Biol Chem. 1990;265:12978-86 pubmed