dnaK

Summary

Gene Symbol: dnaK
Description: chaperone Hsp70, with co-chaperone DnaJ
Alias: ECK0014, JW0013, groPAB, groPC, groPF, grpC, grpF, seg
Species: Escherichia coli str. K-12 substr. MG1655
Products:     dnaK

Top Publications

  1. Haslberger T, Weibezahn J, Zahn R, Lee S, Tsai F, Bukau B, et al. M domains couple the ClpB threading motor with the DnaK chaperone activity. Mol Cell. 2007;25:247-60 pubmed
    The AAA(+) chaperone ClpB mediates the reactivation of aggregated proteins in cooperation with the DnaK chaperone system...
  2. Stevens S, Cai S, Pellecchia M, Zuiderweg E. The solution structure of the bacterial HSP70 chaperone protein domain DnaK(393-507) in complex with the peptide NRLLLTG. Protein Sci. 2003;12:2588-96 pubmed
    ..We present the solution structure of the substrate binding domain (residues 393-507) of the Escherichia coli Hsp70, DnaK, that is bound to the peptide NRLLLTG and compare it to the crystal structure of DnaK(389-607) bound to the same ..
  3. Alix J, Nierhaus K. DnaK-facilitated ribosome assembly in Escherichia coli revisited. RNA. 2003;9:787-93 pubmed
    Assembly helpers exist for the formation of ribosomal subunits. Such a function has been suggested for the DnaK system of chaperones (DnaK, DnaJ, GrpE)...
  4. Qi R, Sarbeng E, Liu Q, Le K, Xu X, Xu H, et al. Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP. Nat Struct Mol Biol. 2013;20:900-7 pubmed publisher
    ..These conformational changes, together with our biochemical assays, provide a structural explanation for allosteric coupling in Hsp70 activity. ..
  5. Bukau B, Walker G. Mutations altering heat shock specific subunit of RNA polymerase suppress major cellular defects of E. coli mutants lacking the DnaK chaperone. EMBO J. 1990;9:4027-36 pubmed
    ..In an effort to understand the roles of molecular chaperones such as DnaK in cellular metabolism, we analyzed secondary mutations (sid) that suppress the growth defects of delta dnaK52 ..
  6. McCarty J, Rudiger S, Schönfeld H, Schneider Mergener J, Nakahigashi K, Yura T, et al. Regulatory region C of the E. coli heat shock transcription factor, sigma32, constitutes a DnaK binding site and is conserved among eubacteria. J Mol Biol. 1996;256:829-37 pubmed
    ..the sensing of stress and transmission of this information to sigma32, involves the chaperone system formed by the DnaK, DnaJ and GrpE heat shock proteins...
  7. Al Refaii A, Alix J. Ribosome biogenesis is temperature-dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ. Mol Microbiol. 2009;71:748-62 pubmed publisher
    In Escherichia coli strains carrying null mutations in either the dnaK or dnaJ genes, the late stages of 30S and 50S ribosomal subunit biogenesis are slowed down in a temperature-dependent manner...
  8. Grimshaw J, Jelesarov I, Siegenthaler R, Christen P. Thermosensor action of GrpE. The DnaK chaperone system at heat shock temperatures. J Biol Chem. 2003;278:19048-53 pubmed
    Temperature directly controls functional properties of the DnaK/DnaJ/GrpE chaperone system...
  9. Zolkiewski M. ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli. J Biol Chem. 1999;274:28083-6 pubmed
    ..02% of the native activity) because of extensive aggregation. Conventional chaperone systems (GroEL/GroES and DnaK/DnaJ/GrpE) or ClpB alone did not reactivate luciferase under those conditions...

More Information

Publications86

  1. El Hage A, Sbai M, Alix J. The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli. Mol Gen Genet. 2001;264:796-808 pubmed
    It has been shown that in Escherichia coli the chaperone DnaK is necessary for the late stages of 50S and 30S ribosomal subunit assembly in vivo...
  2. Chang L, Thompson A, Ung P, Carlson H, Gestwicki J. Mutagenesis reveals the complex relationships between ATPase rate and the chaperone activities of Escherichia coli heat shock protein 70 (Hsp70/DnaK). J Biol Chem. 2010;285:21282-91 pubmed publisher
    The Escherichia coli 70-kDa heat shock protein, DnaK, is a molecular chaperone that engages in a variety of cellular activities, including the folding of proteins...
  3. Gamer J, Bujard H, Bukau B. Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32. Cell. 1992;69:833-42 pubmed
    ..This regulatory function has been postulated for Escherichia coli to rely on the direct association of DnaK (Hsp70) with the heat shock transcription factor sigma 32...
  4. Kanemori M, Mori H, Yura T. Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma 32 in Escherichia coli. J Bacteriol. 1994;176:5648-53 pubmed
    ..Evidence further suggests that the DnaK chaperone plays a crucial regulatory role in induction of the heat shock response by abnormal proteins.
  5. Goloubinoff P, Mogk A, Zvi A, Tomoyasu T, Bukau B. Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network. Proc Natl Acad Sci U S A. 1999;96:13732-7 pubmed
    ..We show here that the sequential action of two Escherichia coli chaperone systems, ClpB and DnaK-DnaJ-GrpE, can efficiently solubilize excess amounts of protein aggregates and refold them into active proteins...
  6. Genevaux P, Georgopoulos C, Kelley W. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol Microbiol. 2007;66:840-57 pubmed
    ..Genome sequencing has revealed that many organisms contain multiple members of both the DnaK (Hsp70) family and their partner J-domain protein (JDP) cochaperone, belonging to the DnaJ (Hsp40) family...
  7. Tomoyasu T, Mogk A, Langen H, Goloubinoff P, Bukau B. Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol. Mol Microbiol. 2001;40:397-413 pubmed
    ..folding assistance by chaperones already at 30 degrees C, and showed strong overlap with previously identified DnaK substrates...
  8. Haslberger T, Zdanowicz A, Brand I, Kirstein J, Turgay K, Mogk A, et al. Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments. Nat Struct Mol Biol. 2008;15:641-50 pubmed publisher
    The ring-forming AAA+ chaperone ClpB cooperates with the DnaK chaperone system to reactivate aggregated proteins...
  9. Grimshaw J, Jelesarov I, Schönfeld H, Christen P. Reversible thermal transition in GrpE, the nucleotide exchange factor of the DnaK heat-shock system. J Biol Chem. 2001;276:6098-104 pubmed
    b>DnaK, a Hsp70 acting in concert with its co-chaperones DnaJ and GrpE, is essential for Escherichia coli to survive environmental stress, including exposure to elevated temperatures...
  10. Swain J, Dinler G, Sivendran R, Montgomery D, Stotz M, Gierasch L. Hsp70 chaperone ligands control domain association via an allosteric mechanism mediated by the interdomain linker. Mol Cell. 2007;26:27-39 pubmed
    ..We have studied single- and two-domain versions of the E. coli Hsp70, DnaK, to explore the mechanism of interdomain communication...
  11. Gamer J, Multhaup G, Tomoyasu T, McCarty J, Rudiger S, Schönfeld H, et al. A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32. EMBO J. 1996;15:607-17 pubmed
    The chaperone system formed by DnaK, DnaJ and GrpE mediates stress-dependent negative modulation of the Escherichia coli heat shock response, probably through association with the heat shock promoter-specific sigma32 subunit of RNA ..
  12. Bork P, Sander C, Valencia A. An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. Proc Natl Acad Sci U S A. 1992;89:7290-4 pubmed
    ..A common evolutionary origin for all of the proteins in this class is proposed. ..
  13. Maki J, Schnobrich D, Culver G. The DnaK chaperone system facilitates 30S ribosomal subunit assembly. Mol Cell. 2002;10:129-38 pubmed
    ..Extract activation of in vitro assembly results in association of DnaK/hsp70 chaperone components with pre-30S particles...
  14. El Hage A, Alix J. Authentic precursors to ribosomal subunits accumulate in Escherichia coli in the absence of functional DnaK chaperone. Mol Microbiol. 2004;51:189-201 pubmed
    Escherichia coli dnaK-ts mutants are defective in the late stages of ribosome biogenesis at high temperature...
  15. Miot M, Reidy M, Doyle S, Hoskins J, Johnston D, Genest O, et al. Species-specific collaboration of heat shock proteins (Hsp) 70 and 100 in thermotolerance and protein disaggregation. Proc Natl Acad Sci U S A. 2011;108:6915-20 pubmed publisher
    ..Hsp104 and ClpB collaborate with the Hsp70 and DnaK chaperone systems, respectively, to retrieve and reactivate stress-denatured proteins from aggregates...
  16. Chang L, Bertelsen E, Wisén S, Larsen E, Zuiderweg E, Gestwicki J. High-throughput screen for small molecules that modulate the ATPase activity of the molecular chaperone DnaK. Anal Biochem. 2008;372:167-76 pubmed
    b>DnaK is a molecular chaperone of Escherichia coli that belongs to a family of conserved 70-kDa heat shock proteins...
  17. Zhuravleva A, Clerico E, Gierasch L. An interdomain energetic tug-of-war creates the allosterically active state in Hsp70 molecular chaperones. Cell. 2012;151:1296-307 pubmed publisher
    ..We have trapped this "allosterically active" state for the E. coli Hsp70, DnaK, and identified how interactions among the NBD, the ? subdomain of the SBD, the SBD ?-helical lid, and the ..
  18. Alix J, Guerin M. Mutant DnaK chaperones cause ribosome assembly defects in Escherichia coli. Proc Natl Acad Sci U S A. 1993;90:9725-9 pubmed
    ..the participation of additional factors, we have studied the influence of a chaperone, the product of the gene dnaK, on ribosome assembly in vivo...
  19. Weibezahn J, Tessarz P, Schlieker C, Zahn R, Maglica Z, Lee S, et al. Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB. Cell. 2004;119:653-65 pubmed
    ..of the ClpB (Hsp104) AAA+ chaperone that solubilizes and reactivates aggregated proteins in concert with the DnaK (Hsp70) chaperone system...
  20. Zhu X, Zhao X, Burkholder W, Gragerov A, Ogata C, Gottesman M, et al. Structural analysis of substrate binding by the molecular chaperone DnaK. Science. 1996;272:1606-14 pubmed
    b>DnaK and other members of the 70-kilodalton heat-shock protein (hsp70) family promote protein folding, interaction, and translocation, both constitutively and in response to stress, by binding to unfolded polypeptide segments...
  21. Reidy M, Miot M, Masison D. Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions. Genetics. 2012;192:185-93 pubmed publisher
    ..Our results define cooperative interactions among these components that are specific or interchangeable across life kingdoms and imply Hsp100 family disaggregases possess intrinsic amyloid remodeling activity. ..
  22. Seoh H, Weech M, Zhang N, Squires C. rRNA antitermination functions with heat shock promoters. J Bacteriol. 2003;185:6486-9 pubmed
    ..ability of RNA polymerase elongation complexes that had initiated at three different heat shock promoters, dnaK, groE, and clpB, and then transcribed the antitermination sequence to read through a Rho-dependent terminator...
  23. Maki J, Southworth D, Culver G. Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system. RNA. 2003;9:1418-21 pubmed
    Recently, there has been controversy regarding the ability of the DnaK chaperone system to facilitate Escherichia coli 30S subunit assembly at otherwise nonpermissive conditions...
  24. Rodriguez F, Arsène Ploetze F, Rist W, Rudiger S, Schneider Mergener J, Mayer M, et al. Molecular basis for regulation of the heat shock transcription factor sigma32 by the DnaK and DnaJ chaperones. Mol Cell. 2008;32:347-58 pubmed publisher
    ..is the stress-dependent inhibition of the sigma(32) subunit of RNA polymerase by reversible association with the DnaK chaperone, mediated by the DnaJ cochaperone...
  25. Bertelsen E, Chang L, Gestwicki J, Zuiderweg E. Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate. Proc Natl Acad Sci U S A. 2009;106:8471-6 pubmed publisher
    b>DnaK is the canonical Hsp70 molecular chaperone protein from Escherichia coli...
  26. Arsene F, Tomoyasu T, Bukau B. The heat shock response of Escherichia coli. Int J Food Microbiol. 2000;55:3-9 pubmed
    ..Major HSPs are molecular chaperones, including DnaK, DnaJ and GrpE, and GroEL and GroES, and proteases. They constitute the two major chaperone systems of E...
  27. Doyle S, Hoskins J, Wickner S. Collaboration between the ClpB AAA+ remodeling protein and the DnaK chaperone system. Proc Natl Acad Sci U S A. 2007;104:11138-44 pubmed
    ..ClpB and Hsp104 are able to dissolve protein aggregates in conjunction with the DnaK/Hsp70 chaperone system, although the roles of the individual chaperones in disaggregation are not well understood...
  28. Bukau B, Horwich A. The Hsp70 and Hsp60 chaperone machines. Cell. 1998;92:351-66 pubmed
  29. Naylor D, Stines A, Hoogenraad N, Høj P. Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the Co-chaperones of specific Hsp70 members. J Biol Chem. 1998;273:21169-77 pubmed
    ..the microsomal and two mitochondrial GrpE-like proteins revealed that they bound specifically to Escherichia coli DnaK, and the complexes formed were not disrupted in the presence of 0...
  30. Dougan D, Mogk A, Bukau B. Protein folding and degradation in bacteria: to degrade or not to degrade? That is the question. Cell Mol Life Sci. 2002;59:1607-16 pubmed
    ..Central to this network are two protein families, the AAA+ and the Hsp70 family. The major Hsp70 chaperone. DnaK, efficiently prevents protein aggregation and supports the refolding of damaged proteins...
  31. Buchberger A, Schroder H, Hesterkamp T, Schönfeld H, Bukau B. Substrate shuttling between the DnaK and GroEL systems indicates a chaperone network promoting protein folding. J Mol Biol. 1996;261:328-33 pubmed
    GroEL and DnaK with their cofactors constitute the major chaperone systems promoting protein folding in the Escherichia coli cytosol...
  32. Suh W, Lu C, Gross C. Structural features required for the interaction of the Hsp70 molecular chaperone DnaK with its cochaperone DnaJ. J Biol Chem. 1999;274:30534-9 pubmed
    ..We have explored the interaction between the Escherichia coli Hsp70 family member, DnaK, and its cochaperone partner DnaJ...
  33. Han W, Christen P. cis-Effect of DnaJ on DnaK in ternary complexes with chimeric DnaK/DnaJ-binding peptides. FEBS Lett. 2004;563:146-50 pubmed
    Chimeric peptides, comprising a DnaK-binding sequence of L-amino acid residues (motif k) and an exclusive DnaJ-binding sequence of D-amino acid residues (motif j) connected through a 22-residue linker, were examined as minisubstrates for ..
  34. Regonesi M, Del Favero M, Basilico F, Briani F, Benazzi L, Tortora P, et al. Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach. Biochimie. 2006;88:151-61 pubmed
    ..By this proteomic approach, we show that the chaperone protein DnaK, previously identified as a "minor component" of the degradosome, associates with abnormal complexes ..
  35. Nunes J, Mayer Hartl M, Hartl F, Müller D. Action of the Hsp70 chaperone system observed with single proteins. Nat Commun. 2015;6:6307 pubmed publisher
    In Escherichia coli, the binding of non-native protein substrates to the Hsp70 chaperone DnaK is mediated by the co-chaperone DnaJ. DnaJ accelerates ATP hydrolysis on DnaK, by closing the peptide-binding cleft of DnaK...
  36. Rokney A, Shagan M, Kessel M, Smith Y, Rosenshine I, Oppenheim A. E. coli transports aggregated proteins to the poles by a specific and energy-dependent process. J Mol Biol. 2009;392:589-601 pubmed publisher
    ..The latter steps require the proton motive force, activities of the DnaK and DnaJ chaperones, and MreB...
  37. Zhao X, Braun A, Braun J. Biological roles of neural J proteins. Cell Mol Life Sci. 2008;65:2385-96 pubmed publisher
    ..We are beginning to gain some insights into the neuronal network of J proteins. Here, we highlight recent advances in our understanding of how select J proteins harness Hsp70 s for fundamentally important conformational work in neurons. ..
  38. Hansen S, Lewis K, Vulic M. Role of global regulators and nucleotide metabolism in antibiotic tolerance in Escherichia coli. Antimicrob Agents Chemother. 2008;52:2718-26 pubmed publisher
    ..The genes affected in these strains were dnaJ and dnaK (chaperones), apaH (diadenosine tetraphosphatase), surA (peptidyl-prolyl cis-trans isomerase), fis and hns (global ..
  39. Liberek K, Marszalek J, Ang D, Georgopoulos C, Zylicz M. Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK. Proc Natl Acad Sci U S A. 1991;88:2874-8 pubmed
    The products of the Escherichia coli dnaK, dnaJ, and grpE heat shock genes have been previously shown to be essential for bacteriophage lambda DNA replication at all temperatures and for bacterial survival under certain conditions...
  40. Neidhardt F, VanBogelen R. Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem Biophys Res Commun. 1981;100:894-900 pubmed
  41. McCarty J, Walker G. DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity. Proc Natl Acad Sci U S A. 1991;88:9513-7 pubmed
    b>DnaK, the sole Escherichia coli member of the highly conserved 70-kDa heat shock protein (HSP70) family of proteins, autophosphorylates when incubated with ATP in vitro...
  42. Ohki M, Tamura F, Nishimura S, Uchida H. Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product. J Biol Chem. 1986;261:1778-81 pubmed
    The dnaJ and dnaK genes are essential for replication of Escherichia coli DNA, and they constitute an operon, dnaJ being downstream from dnaK. The amount of the dnaJ protein in E...
  43. Barthel T, Zhang J, Walker G. ATPase-defective derivatives of Escherichia coli DnaK that behave differently with respect to ATP-induced conformational change and peptide release. J Bacteriol. 2001;183:5482-90 pubmed
    ..of the T199S, T199A, and K70A mutations on the biochemical activity and in vivo functioning of Escherichia coli DnaK. Threonine-199 is the site of autophosphorylation of DnaK, and the lysine residue of bovine Hsc70 corresponding to ..
  44. Ferrer M, Chernikova T, Yakimov M, Golyshin P, Timmis K. Chaperonins govern growth of Escherichia coli at low temperatures. Nat Biotechnol. 2003;21:1266-7 pubmed
  45. Wetzstein M, Schumann W. Promoters of major Escherichia coli heat shock genes seem non-functional in Bacillus subtilis. FEMS Microbiol Lett. 1990;60:55-8 pubmed
    ..subtilis. This indicates that E. coli heat shock promoters are nonfunctional in B. subtilis. ..
  46. Echtenkamp P, Wilson D, Shuler M. Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design. Biotechnol Bioeng. 2009;102:902-9 pubmed publisher
    ..In conclusion, gene position, with regard to the C period, and gene function are important factors to incorporate into design criteria for synthetic bacterial genomes. ..
  47. Wild J, Altman E, Yura T, Gross C. DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli. Genes Dev. 1992;6:1165-72 pubmed
    ..We report results indicating that the DnaK and DnaJ heat shock proteins are also involved in the export of several proteins, most likely by acting as their ..
  48. Chiappori F, Merelli I, Milanesi L, Colombo G, Morra G. An atomistic view of Hsp70 allosteric crosstalk: from the nucleotide to the substrate binding domain and back. Sci Rep. 2016;6:23474 pubmed publisher
    ..drives the linker docking by increasing the local dynamical coordination of its C-terminal end: a partially docked DnaK structure is achieved by combining ATP in the NBD and peptide in the SBD...
  49. Martínez Alonso M, Vera A, Villaverde A. Role of the chaperone DnaK in protein solubility and conformational quality in inclusion body-forming Escherichia coli cells. FEMS Microbiol Lett. 2007;273:187-95 pubmed
    ..Therefore, coproducing DnaK or other main chaperones along with the target protein has been a common approach to gain solubility, although with ..
  50. Sugimoto S, Saruwatari K, Higashi C, Sonomoto K. The proper ratio of GrpE to DnaK is important for protein quality control by the DnaK-DnaJ-GrpE chaperone system and for cell division. Microbiology. 2008;154:1876-85 pubmed publisher
    ..but overexpression of GrpE-G122D, which carries the G122D point mutation resulting in impaired interaction with DnaK, did not; this indicated that the effect of GrpE overexpression could be related to the DnaK chaperone function...
  51. Kadibalban A, Bogumil D, Landan G, Dagan T. DnaK-Dependent Accelerated Evolutionary Rate in Prokaryotes. Genome Biol Evol. 2016;8:1590-9 pubmed publisher
    ..Here, we study the impact of DnaK (Hsp70) chaperone on the evolution of its client proteins...
  52. Alfano C, McMacken R. Ordered assembly of nucleoprotein structures at the bacteriophage lambda replication origin during the initiation of DNA replication. J Biol Chem. 1989;264:10699-708 pubmed
    ..The E. coli DnaK heat shock protein can bind to any of these early stage nucleoprotein structures, and in a fourth-stage reaction a ..
  53. Wickner S, Skowyra D, Hoskins J, McKenney K. DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step. Proc Natl Acad Sci U S A. 1992;89:10345-9 pubmed
    We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA...
  54. Slepenkov S, Witt S. Kinetic analysis of interdomain coupling in a lidless variant of the molecular chaperone DnaK: DnaK's lid inhibits transition to the low affinity state. Biochemistry. 2002;41:12224-35 pubmed
    b>DnaK, the Escherichia coli Hsp70, possesses two functional domains, the N- and C-terminal ATPase and peptide-binding domains, respectively...
  55. Al Refaii A, Alix J. Inhibition of chaperone-dependent bacterial ribosome biogenesis. Methods Mol Med. 2008;142:75-85 pubmed publisher
    In Escherichia coli, the molecular chaperone HSP70 (DnaK) is necessary for 30S and 50S ribosomal subunit assembly at temperatures above 37 degrees C...
  56. Chow I, Barnett M, Zolkiewski M, Baneyx F. The N-terminal domain of Escherichia coli ClpB enhances chaperone function. FEBS Lett. 2005;579:4242-8 pubmed
    ..Our results are consistent with a model in which the N-domain of ClpB95 maximizes substrate processing under conditions where the cellular supply of free DnaK-DnaJ is limiting.
  57. Hwang D, Crooke E, Kornberg A. Aggregated dnaA protein is dissociated and activated for DNA replication by phospholipase or dnaK protein. J Biol Chem. 1990;265:19244-8 pubmed
    ..Replication activity of the aggregated form can be restored by treatments with either dnaK protein or phospholipase A2...
  58. Laufen T, Mayer M, Beisel C, Klostermeier D, Mogk A, Reinstein J, et al. Mechanism of regulation of hsp70 chaperones by DnaJ cochaperones. Proc Natl Acad Sci U S A. 1999;96:5452-7 pubmed
    ..We show that DnaJ stimulates ATP hydrolysis by Escherichia coli Hsp70, DnaK, very efficiently to >1000-fold, but only if present at high (micromolar) concentration...
  59. Zmijewski M, Kwiatkowska J, Lipinska B. Complementation studies of the DnaK-DnaJ-GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro. Arch Microbiol. 2004;182:436-49 pubmed
    ..The DnaK-DnaJ-GrpE chaperone machinery of V...
  60. De Spiegeleer P, Sermon J, Lietaert A, Aertsen A, Michiels C. Source of tryptone in growth medium affects oxidative stress resistance in Escherichia coli. J Appl Microbiol. 2004;97:124-33 pubmed
    ..This work highlights the importance of controlling very subtle differences in composition of nonselective growth media in studies on bacterial physiology. ..
  61. Landry S. Structure and energetics of an allele-specific genetic interaction between dnaJ and dnaK: correlation of nuclear magnetic resonance chemical shift perturbations in the J-domain of Hsp40/DnaJ with binding affinity for the ATPase domain of Hsp70/DnaK. Biochemistry. 2003;42:4926-36 pubmed
    The molecular chaperone machine composed of Escherichia coli Hsp70/DnaK and Hsp40/DnaJ binds and releases client proteins in cycles of ATP-dependent protein folding, membrane translocation, disassembly, and degradation...
  62. Liebscher M, Roujeinikova A. Allosteric coupling between the lid and interdomain linker in DnaK revealed by inhibitor binding studies. J Bacteriol. 2009;191:1456-62 pubmed publisher
    The molecular chaperone DnaK assists protein folding and refolding, translocation across membranes, and regulation of the heat shock response...
  63. Patra M, Roy S, Dasgupta R, Basu T. GroEL to DnaK chaperone network behind the stability modulation of σ(32) at physiological temperature in Escherichia coli. FEBS Lett. 2015;589:4047-52 pubmed publisher
    ..factor σ(32) in Escherichia coli has long been known to be modulated only by its own transcribed chaperone DnaK. Very few reports suggest a role for another heat-shock chaperone, GroEL, for maintenance of cellular σ(32) ..
  64. Swain J, Schulz E, Gierasch L. Direct comparison of a stable isolated Hsp70 substrate-binding domain in the empty and substrate-bound states. J Biol Chem. 2006;281:1605-11 pubmed
    ..We have generated a new stable construct of the substrate-binding domain from the Escherichia coli Hsp70, DnaK, which has enabled us to compare the empty and peptide-bound conformations using NMR chemical shift analysis and ..
  65. Bardwell J, Craig E. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous. Proc Natl Acad Sci U S A. 1984;81:848-52 pubmed
    The Escherichia coli dnaK gene is homologous to the major heat shock-induced gene in Drosophila (Hsp70)...
  66. Singh B, Gupta R. Conserved inserts in the Hsp60 (GroEL) and Hsp70 (DnaK) proteins are essential for cellular growth. Mol Genet Genomics. 2009;281:361-73 pubmed publisher
    ..A one aa insert in the E. coli GroEL and a 21-23 insert in the DnaK proteins are specific for most Gram-negative bacteria...
  67. Zolkiewski M, Chesnokova L, Witt S. Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-Chaperone System. Curr Protoc Protein Sci. 2016;83:28.10.1-28.10.18 pubmed publisher
    ..reactivation of an aggregated enzyme glucose-6-phosphate dehydrogenase mediated by ClpB from Escherichia coli in cooperation with another molecular chaperone, DnaK. The procedures for purification of these chaperones are also described.
  68. Tomoyasu T, Ogura T, Tatsuta T, Bukau B. Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli. Mol Microbiol. 1998;30:567-81 pubmed
    ..Modulators are the DnaK chaperone system, which inactivates and destabilizes sigma32, and the FtsH protease, which is largely responsible ..
  69. Witt S. Tethering creates unusual kinetics for ribosome-associated chaperones with nascent chains. Protein Pept Lett. 2009;16:631-4 pubmed
    ..Ribosome-bound chaperones bind nascent chains intramolecularly with rates as large as 10(4) s(-1) in order to keep chains unfolded. ..
  70. Leskovar A, Reinstein J. Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing. Arch Biochem Biophys. 2008;473:16-24 pubmed publisher
    ..The measured quantum yields quantify the increase in fluorescence for (C8)-MABA-ADP, MANT-ATP and (Pgamma)-MABA-ATP as 153%, 93% and 14% when bound to DnaK, ClpB and Trap1, respectively, compared to free in buffer solution.
  71. Otvos L, O I, Rogers M, Consolvo P, Condie B, Lovas S, et al. Interaction between heat shock proteins and antimicrobial peptides. Biochemistry. 2000;39:14150-9 pubmed
    ..to mass spectrometry, Western blot, and fluorescence polarization, the short, proline-rich peptides bound to DnaK, the 70-kDa bacterial heat shock protein, both in solution and on the solid-phase...
  72. Okada S, Okada T, Aimi T, Morinaga T, Itoh T. HSP70 and ribosomal protein L2: novel 5S rRNA binding proteins in Escherichia coli. FEBS Lett. 2000;485:153-6 pubmed
    ..In addition, the interaction of these proteins with 5S rRNA has been confirmed with gel mobility shift assays. ..
  73. Sekhar A, Nagesh J, Rosenzweig R, Kay L. Conformational heterogeneity in the Hsp70 chaperone-substrate ensemble identified from analysis of NMR-detected titration data. Protein Sci. 2017;26:2207-2220 pubmed publisher
    ..We have recently shown by methyl-TROSY NMR methods that the Escherichia coli Hsp70, DnaK, can form multiple bound complexes with a small client protein, hTRF1...
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    ..The role played by the major molecular chaperone teams DnaK-DnaJ-GrpE and GroEL-GroES on the productivity of recombinant streptokinase was experimentally determined...
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    ..Furthermore, we demonstrate that bacterial lysine acetylation is regulated in response to stress stimuli. ..
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    ..Among these factors, the chaperone DnaK is of primary importance for preserving the integrity of epsilon...
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    ..P.DnaB.ori lambda complex. Addition of the DnaK and DnaJ proteins yields a third stage complex containing DnaK, DnaJ, O, P, and DnaB...