dnaJ

Summary

Gene Symbol: dnaJ
Description: chaperone Hsp40, DnaK co-chaperone
Alias: ECK0015, JW0014, faa, groP, grpC
Species: Escherichia coli str. K-12 substr. MG1655
Products:     dnaJ

Top Publications

  1. Gur E, Katz C, Ron E. All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins. FEBS Lett. 2005;579:1935-9 pubmed
    b>DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well...
  2. Srinivasan S, Gillies A, Chang L, Thompson A, Gestwicki J. Molecular chaperones DnaK and DnaJ share predicted binding sites on most proteins in the E. coli proteome. Mol Biosyst. 2012;8:2323-33 pubmed publisher
    In Escherichia coli, the molecular chaperones DnaK and DnaJ cooperate to assist the folding of newly synthesized or unfolded polypeptides. DnaK and DnaJ bind to hydrophobic motifs in these proteins and they also bind to each other...
  3. Gamer J, Bujard H, Bukau B. Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32. Cell. 1992;69:833-42 pubmed
    ..This report presents evidence for the physical association of DnaK, DnaJ, and GrpE chaperones with sigma 32 in vivo...
  4. Maki J, Schnobrich D, Culver G. The DnaK chaperone system facilitates 30S ribosomal subunit assembly. Mol Cell. 2002;10:129-38 pubmed
    ..Purified DnaK, its cochaperones DnaJ and GrpE, and ATP can facilitate reconstitution of functional 30S subunits under otherwise nonpermissive conditions...
  5. Wada M, Sekine K, Itikawa H. Participation of the dnaK and dnaJ gene products in phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase of Escherichia coli K-12. J Bacteriol. 1986;168:213-20 pubmed
    The heat shock proteins DnaK and DnaJ of Escherichia coli participate in phosphorylation of both glutaminyl-tRNA synthetase and threonyl-tRNA synthetase...
  6. Saito H, Uchida H. Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12. J Mol Biol. 1977;113:1-25 pubmed
  7. Wild J, Altman E, Yura T, Gross C. DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli. Genes Dev. 1992;6:1165-72 pubmed
    ..We report results indicating that the DnaK and DnaJ heat shock proteins are also involved in the export of several proteins, most likely by acting as their chaperones...
  8. Houry W. Chaperone-assisted protein folding in the cell cytoplasm. Curr Protein Pept Sci. 2001;2:227-44 pubmed
    ..2) The Hsp 70 system consisting of DnaK (Hsp 70), its cofactor DnaJ (Hsp 40), and the nucleotide exchange factor GrpE...
  9. Kluck C, Patzelt H, Genevaux P, Brehmer D, Rist W, Schneider Mergener J, et al. Structure-function analysis of HscC, the Escherichia coli member of a novel subfamily of specialized Hsp70 chaperones. J Biol Chem. 2002;277:41060-9 pubmed
    ..The HscC ATPase was not affected by the nucleotide exchange factor of DnaK GrpE and stimulated 8-fold by DjlC, a DnaJ protein with a putative transmembrane domain, but not by other DnaJ proteins tested...

More Information

Publications100

  1. Shi Y, Tang W, Hao S, Wang C. Contributions of cysteine residues in Zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone DnaJ. Biochemistry. 2005;44:1683-9 pubmed
    Escherichia coli DnaJ, possessing both chaperone and thiol-disulfide oxidoreductase activities, is a homodimeric Hsp40 protein...
  2. Genevaux P, Schwager F, Georgopoulos C, Kelley W. Scanning mutagenesis identifies amino acid residues essential for the in vivo activity of the Escherichia coli DnaJ (Hsp40) J-domain. Genetics. 2002;162:1045-53 pubmed
    The DnaJ (Hsp40) cochaperone regulates the DnaK (Hsp70) chaperone by accelerating ATP hydrolysis in a cycle closely linked to substrate binding and release...
  3. Gamer J, Multhaup G, Tomoyasu T, McCarty J, Rudiger S, Schönfeld H, et al. A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32. EMBO J. 1996;15:607-17 pubmed
    The chaperone system formed by DnaK, DnaJ and GrpE mediates stress-dependent negative modulation of the Escherichia coli heat shock response, probably through association with the heat shock promoter-specific sigma32 subunit of RNA ..
  4. Liberek K, Marszalek J, Ang D, Georgopoulos C, Zylicz M. Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK. Proc Natl Acad Sci U S A. 1991;88:2874-8 pubmed
    The products of the Escherichia coli dnaK, dnaJ, and grpE heat shock genes have been previously shown to be essential for bacteriophage lambda DNA replication at all temperatures and for bacterial survival under certain conditions...
  5. Wetzstein M, Schumann W. Promoters of major Escherichia coli heat shock genes seem non-functional in Bacillus subtilis. FEMS Microbiol Lett. 1990;60:55-8 pubmed
    ..subtilis. This indicates that E. coli heat shock promoters are nonfunctional in B. subtilis. ..
  6. Martinez Yamout M, Legge G, Zhang O, Wright P, Dyson H. Solution structure of the cysteine-rich domain of the Escherichia coli chaperone protein DnaJ. J Mol Biol. 2000;300:805-18 pubmed
    The solution structure of the cysteine-rich (CR) domain of Escherichia coli DnaJ has been solved by NMR methods. The structure of a 79 residue CR domain construct shows a novel fold with an overall V-shaped extended beta-hairpin topology...
  7. Tatsuta T, Tomoyasu T, Bukau B, Kitagawa M, Mori H, Karata K, et al. Heat shock regulation in the ftsH null mutant of Escherichia coli: dissection of stability and activity control mechanisms of sigma32 in vivo. Mol Microbiol. 1998;30:583-93 pubmed
    ..In addition, CbpA, an analogue of DnaJ, was demonstrated to have overlapping functions with DnaJ in both the activity and the stability control of sigma32. ..
  8. Nardo L, Nikas G, Makrigiannakis A, Sinatra F, Nardo F. Synchronous expression of pinopodes and alpha v beta 3 and alpha 4 beta 1 integrins in the endometrial surface epithelium of normally menstruating women during the implantation window. J Reprod Med. 2003;48:355-61 pubmed
    ..These results indicate a coordinated, synchronous change in morphology and adhesion molecule expression of endometrial epithelial cells on day LH +8 of a normal menstrual cycle. ..
  9. Zhu X, Zhao X, Burkholder W, Gragerov A, Ogata C, Gottesman M, et al. Structural analysis of substrate binding by the molecular chaperone DnaK. Science. 1996;272:1606-14 pubmed
    ..This domain is rotated in the molecules of a second crystal lattice, which suggests a model of conformation-dependent substrate binding that features a latch mechanism for maintaining long lifetime complexes. ..
  10. Georgopoulos C. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase. Proc Natl Acad Sci U S A. 1971;68:2977-81 pubmed
    ..This suggests that the groN mutation produces a structural change in the bacterial RNA polymerase such that it can no longer interact properly with the phage N product. ..
  11. Blumberg H, Silver P. A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast. Nature. 1991;349:627-30 pubmed
    ..In Escherichia coli, the heat-shock protein DnaJ and the Hsp70 homologue, DnaK, act together to disassemble a protein complex involved in bacteriophage lambda ..
  12. Puvirajesinghe T, ElAntak L, Lignon S, Franche N, Ilbert M, Ansaldi M. DnaJ (Hsp40 protein) binding to folded substrate impacts KplE1 prophage excision efficiency. J Biol Chem. 2012;287:14169-77 pubmed publisher
    ..We have previously shown that DnaJ promotes KplE1 excision by increasing the affinity of TorI for its site-specific recombination DNA target...
  13. Straus D, Walter W, Gross C. DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of sigma 32. Genes Dev. 1990;4:2202-9 pubmed
    ..coli heat shock gene expression. We report that two other heat shock proteins, DnaJ and GrpE, are also involved in the negative regulation of heat shock gene expression...
  14. Al Refaii A, Alix J. Ribosome biogenesis is temperature-dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ. Mol Microbiol. 2009;71:748-62 pubmed publisher
    In Escherichia coli strains carrying null mutations in either the dnaK or dnaJ genes, the late stages of 30S and 50S ribosomal subunit biogenesis are slowed down in a temperature-dependent manner...
  15. Ohki R, Kawamata T, Katoh Y, Hosoda F, Ohki M. Escherichia coli dnaJ deletion mutation results in loss of stability of a positive regulator, CRP. J Biol Chem. 1992;267:13180-4 pubmed
    The dnaJ deletion mutant K7052(lambda dnaK) has a temperature-sensitive defect in the synthesis of beta-galactosidase...
  16. Kim S, Sharma S, Hoskins J, Wickner S. Interaction of the DnaK and DnaJ chaperone system with a native substrate, P1 RepA. J Biol Chem. 2002;277:44778-83 pubmed
    ..chaperone of Escherichia coli interacts with protein substrates in an ATP-dependent manner, in conjunction with DnaJ and GrpE co-chaperones, to carry out protein folding, protein remodeling, and assembly and disassembly of ..
  17. Perales Calvo J, Muga A, Moro F. Role of DnaJ G/F-rich domain in conformational recognition and binding of protein substrates. J Biol Chem. 2010;285:34231-9 pubmed publisher
    b>DnaJ from Escherichia coli is a Type I Hsp40 that functions as a cochaperone of DnaK (Hsp70), stimulating its ATPase activity and delivering protein substrates. How DnaJ binds protein substrates is still poorly understood...
  18. Wickner S, Hoskins J, McKenney K. Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA. Nature. 1991;350:165-7 pubmed
    ..The Escherichia coli hsp70 analogue, DnaK, and two other heat-shock proteins, DnaJ and GrpE, are required for cell viability at high temperatures and are involved in DNA replication of phage lambda ..
  19. Schroder H, Langer T, Hartl F, Bukau B. DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage. EMBO J. 1993;12:4137-44 pubmed
    ..We investigated the roles of the DnaK (Hsp70), DnaJ and GrpE chaperones of Escherichia coli in prevention and repair of thermally induced protein damage using firefly ..
  20. Liberek K, Wall D, Georgopoulos C. The DnaJ chaperone catalytically activates the DnaK chaperone to preferentially bind the sigma 32 heat shock transcriptional regulator. Proc Natl Acad Sci U S A. 1995;92:6224-8 pubmed
    ..Recently, we have shown that the DnaK and DnaJ proteins can compete with RNA polymerase for binding to the sigma 32 transcription factor in the presence of ATP, ..
  21. Horne B, Li T, Genevaux P, Georgopoulos C, Landry S. The Hsp40 J-domain stimulates Hsp70 when tethered by the client to the ATPase domain. J Biol Chem. 2010;285:21679-88 pubmed publisher
    The Escherichia coli Hsp40 DnaJ uses its J-domain (Jd) to couple ATP hydrolysis and client protein capture in Hsp70 DnaK...
  22. Mogk A, Deuerling E, Vorderwülbecke S, Vierling E, Bukau B. Small heat shock proteins, ClpB and the DnaK system form a functional triade in reversing protein aggregation. Mol Microbiol. 2003;50:585-95 pubmed
    ..The DnaK requirement for growth is increasingly higher for delta ibpAB, delta clpB, and the double delta ibpAB delta clpB mutant cells, establishing the positions of sHsps and ClpB in this chaperone triade. ..
  23. de Crouy Chanel A, Kohiyama M, Richarme G. A novel function of Escherichia coli chaperone DnaJ. Protein-disulfide isomerase. J Biol Chem. 1995;270:22669-72 pubmed
    ..The DnaJ protein of Escherichia coli and the DnaJ-like proteins of eukaryotes are known as molecular chaperones and specific ..
  24. Noguchi A, Ikeda A, Mezaki M, Fukumori Y, Kanemori M. DnaJ-promoted binding of DnaK to multiple sites on ?32 in the presence of ATP. J Bacteriol. 2014;196:1694-703 pubmed publisher
    ..Two ?(32) mutants, L201D ?(32) and I54A ?(32), which have reduced affinities for DnaK and DnaJ (Hsp40), respectively, were used to further characterize DnaK-?(32) complex formation...
  25. Gur E, Biran D, Shechter N, Genevaux P, Georgopoulos C, Ron E. The Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation. J Bacteriol. 2004;186:7236-42 pubmed
    The DnaJ (Hsp40) protein of Escherichia coli serves as a cochaperone of DnaK (Hsp70), whose activity is involved in protein folding, protein targeting for degradation, and rescue of proteins from aggregates. Two other E...
  26. Grudniak A, WÅ‚odkowska J, Wolska K. Chaperone DnaJ Influences the Formation of Biofilm by Escherichia coli. Pol J Microbiol. 2015;64:279-83 pubmed
    b>DnaJ chaperone, a member of the so called DnaK-DnaJ-GrpE chaperone machine plays an important role in cell physiology. The ability of Escherichia coli ΔdnaJ mutant to form biofilm was studied...
  27. Grimshaw J, Jelesarov I, Schönfeld H, Christen P. Reversible thermal transition in GrpE, the nucleotide exchange factor of the DnaK heat-shock system. J Biol Chem. 2001;276:6098-104 pubmed
    DnaK, a Hsp70 acting in concert with its co-chaperones DnaJ and GrpE, is essential for Escherichia coli to survive environmental stress, including exposure to elevated temperatures...
  28. Acebron S, Martín I, Del Castillo U, Moro F, Muga A. DnaK-mediated association of ClpB to protein aggregates. A bichaperone network at the aggregate surface. FEBS Lett. 2009;583:2991-6 pubmed publisher
    ..We analyzed here the interaction of DnaJ/DnaK and ClpB with protein aggregates...
  29. Nian R, Kim D, Tan L, Kim C, Choe W. Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase. Biotechnol Prog. 2009;25:1078-85 pubmed publisher
  30. Grudniak A, Kuć M, Wolska K. Role of Escherichia coli DnaK and DnaJ chaperones in spontaneous and induced mutagenesis and their effect on UmuC stability. FEMS Microbiol Lett. 2005;242:361-6 pubmed
    ..Reduced UmuC stability was demonstrated only in the DeltadnaKdnaJ mutant. ..
  31. Sunshine M, Feiss M, Stuart J, Yochem J. A new host gene (groPC) necessary for lambda DNA replication. Mol Gen Genet. 1977;151:27-34 pubmed
    ..The gro mutation, groPC259, is recessive to wild type and maps between threonine (thr) and diaminopimelate (dapB) on the E. coli chromosome. The possibility that the groPC gene is concerned with host DNA replication is discussed. ..
  32. Yamagata H, Taguchi N, Daishima K, Mizushima S. Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli. Mol Gen Genet. 1983;192:10-4 pubmed
    ..The mutation was mapped in the dnaJ-rpsT-ileS-dapB region by interrupted mating with various Hfr strains and P1 phage transduction...
  33. Paetz W, Nass G. Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12. Eur J Biochem. 1973;35:331-7 pubmed
  34. Mel kina O, Gorianin I, Manukhov I, Zavil gel skiĭ G. [Trigger factor dependent refolding of bacterial luciferases in Escherichia coli cells: kinetics, efficiency and effect of the bichaperone system, DnaKJE-ClpB]. Mol Biol (Mosk). 2013;47:492-7 pubmed
    ..The negative effect of the ClpB protein on the TF-dependent refolding was shown: in Escherichia coli clpB::kan TF-dependent refolding is more efficient than in the E. coli clpB+. ..
  35. Linke K, Wolfram T, Bussemer J, Jakob U. The roles of the two zinc binding sites in DnaJ. J Biol Chem. 2003;278:44457-66 pubmed
    All type I DnaJ (Hsp40) homologues share the presence of two highly conserved zinc centers...
  36. Thomas J, Baneyx F. Protein folding in the cytoplasm of Escherichia coli: requirements for the DnaK-DnaJ-GrpE and GroEL-GroES molecular chaperone machines. Mol Microbiol. 1996;21:1185-96 pubmed
    ..investigated the influence of mutations in the sigma(32) heat-shock transcription factor and the DnaK-DnaJ-GrpE and GroEL-GroES molecular chaperone machines on the folding of preS2-beta-galactosidase...
  37. Nam S, Walsh M. Characterization of interactions between Escherichia coli molecular chaperones and immobilized caseins. Prep Biochem Biotechnol. 2003;33:321-39 pubmed
    ..Western analysis identified five E. coli molecular chaperones including DnaK, DnaJ, GrpE, GroEL, and GroES in eluates...
  38. Lund P. Microbial molecular chaperones. Adv Microb Physiol. 2001;44:93-140 pubmed
    ..Other examples of regulation of molecular chaperones will also be discussed. Finally, the likely future research directions for molecular chaperone biology in the post-genomic era will be briefly evaluated. ..
  39. Hoffmann H, Lyman S, Lu C, Petit M, Echols H. Activity of the Hsp70 chaperone complex--DnaK, DnaJ, and GrpE--in initiating phage lambda DNA replication by sequestering and releasing lambda P protein. Proc Natl Acad Sci U S A. 1992;89:12108-11 pubmed
    ..In the disassembly pathway, a set of Escherichia coli heat shock proteins termed the Hsp70 complex--DnaK, DnaJ, and GrpE--act with ATP to release lambda P protein from the nucleo-protein complex, freeing the DnaB helicase for ..
  40. Hansen S, Lewis K, Vulic M. Role of global regulators and nucleotide metabolism in antibiotic tolerance in Escherichia coli. Antimicrob Agents Chemother. 2008;52:2718-26 pubmed publisher
    ..The genes affected in these strains were dnaJ and dnaK (chaperones), apaH (diadenosine tetraphosphatase), surA (peptidyl-prolyl cis-trans isomerase), fis and hns ..
  41. Ying B, Taguchi H, Ueda H, Ueda T. Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system. Biochem Biophys Res Commun. 2004;320:1359-64 pubmed
    ..In addition, both systems also acted as chaperones after translation had been stopped. In contrast, the GroEL/ES system showed little or no co- or post-translational assistance in folding. ..
  42. Greene M, Steede N, Landry S. Domain-specific spectroscopy of 5-hydroxytryptophan-containing variants of Escherichia coli DnaJ. Biochim Biophys Acta. 2000;1480:267-77 pubmed
    Tryptophan-containing variants of Escherichia coli DnaJ protein were constructed in order to examine the hypothetical domain structure by fluorescence quenching and denaturant-induced unfolding...
  43. Wall D, Zylicz M, Georgopoulos C. The NH2-terminal 108 amino acids of the Escherichia coli DnaJ protein stimulate the ATPase activity of DnaK and are sufficient for lambda replication. J Biol Chem. 1994;269:5446-51 pubmed
    The Escherichia coli heat shock proteins DnaK and DnaJ function cooperatively as molecular chaperones. Central to their biochemical functions is the ability of DnaJ to interact with DnaK and to stimulate its ATPase activity...
  44. Sugimoto S, Saruwatari K, Higashi C, Sonomoto K. The proper ratio of GrpE to DnaK is important for protein quality control by the DnaK-DnaJ-GrpE chaperone system and for cell division. Microbiology. 2008;154:1876-85 pubmed publisher
    ..An in vitro luciferase-refolding activity assay using purified DnaK, DnaJ and GrpE proteins demonstrated that high concentrations of GrpE significantly inhibited DnaK-mediated refolding...
  45. Zolkiewski M. ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli. J Biol Chem. 1999;274:28083-6 pubmed
    ..02% of the native activity) because of extensive aggregation. Conventional chaperone systems (GroEL/GroES and DnaK/DnaJ/GrpE) or ClpB alone did not reactivate luciferase under those conditions...
  46. Rokney A, Shagan M, Kessel M, Smith Y, Rosenshine I, Oppenheim A. E. coli transports aggregated proteins to the poles by a specific and energy-dependent process. J Mol Biol. 2009;392:589-601 pubmed publisher
    ..The latter steps require the proton motive force, activities of the DnaK and DnaJ chaperones, and MreB...
  47. de Marco A. Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. Nat Protoc. 2007;2:2632-9 pubmed
    Eight combinations of molecular chaperones (e.g., DnaK/DnaJ/GrpE/ClpB) are co-expressed with the target recombinant protein to compare their effectiveness in improving its soluble yield...
  48. Zzaman S, Reddy J, Bastia D. The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation. J Biol Chem. 2004;279:50886-94 pubmed
    ..Here we show that the chaperone DnaK along with its co-chaperone DnaJ and the nucleotide exchange factor GrpE were needed to activate WT pi and caused it to initiate replication in ..
  49. Chuang S, Blattner F. Characterization of twenty-six new heat shock genes of Escherichia coli. J Bacteriol. 1993;175:5242-52 pubmed
    ..eight examined new proteins were found to be similar to those of the four most studied heat shock proteins, DnaK, DnaJ, GroEL (MopA), and GroES (MopB)...
  50. El Hage A, Sbai M, Alix J. The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli. Mol Gen Genet. 2001;264:796-808 pubmed
    ..Interestingly, overproduction of GroES/GroEL can partially compensate for a lack of DnaK/DnaJ at 44 degrees C.
  51. Zietkiewicz S, Krzewska J, Liberek K. Successive and synergistic action of the Hsp70 and Hsp100 chaperones in protein disaggregation. J Biol Chem. 2004;279:44376-83 pubmed
    ..Bacterial Hsp70 (DnaK), its co-chaperones (DnaJ and GrpE), and Hsp100 (ClpB) were incubated with aggregated GFP, and the increase in GFP fluorescence was monitored...
  52. Jubete Y, Maurizi M, Gottesman S. Role of the heat shock protein DnaJ in the lon-dependent degradation of naturally unstable proteins. J Biol Chem. 1996;271:30798-803 pubmed
    We have investigated the role of DnaJ in protein degradation by examining the degradation of intrinsically unstable proteins by Lon protease in vivo...
  53. Madhusudan S, Paukner A, Klingen Y, Schnetz K. Independent regulation of H-NS-mediated silencing of the bgl operon at two levels: upstream by BglJ and LeuO and downstream by DnaKJ. Microbiology. 2005;151:3349-59 pubmed
    ..Together, the data show that the two levels of bgl silencing by H-NS are regulated independently. ..
  54. Ohki M, Tamura F, Nishimura S, Uchida H. Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product. J Biol Chem. 1986;261:1778-81 pubmed
    The dnaJ and dnaK genes are essential for replication of Escherichia coli DNA, and they constitute an operon, dnaJ being downstream from dnaK. The amount of the dnaJ protein in E...
  55. Chandrangsu P, Wang L, Choi S, Gourse R. Suppression of a dnaKJ deletion by multicopy dksA results from non-feedback-regulated transcripts that originate upstream of the major dksA promoter. J Bacteriol. 2012;194:1437-46 pubmed publisher
  56. Nillegoda N, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, et al. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. elife. 2017;6: pubmed publisher
    ..This fundamental change in J-protein biology during the prokaryote-to-eukaryote transition allows for increased fine-tuning and broadening of Hsp70 function in eukaryotes. ..
  57. Landry S. Structure and energetics of an allele-specific genetic interaction between dnaJ and dnaK: correlation of nuclear magnetic resonance chemical shift perturbations in the J-domain of Hsp40/DnaJ with binding affinity for the ATPase domain of Hsp70/DnaK. Biochemistry. 2003;42:4926-36 pubmed
    The molecular chaperone machine composed of Escherichia coli Hsp70/DnaK and Hsp40/DnaJ binds and releases client proteins in cycles of ATP-dependent protein folding, membrane translocation, disassembly, and degradation...
  58. Hu B, Tomita M. The Hsp70 chaperone system maintains high concentrations of active proteins and suppresses ATP consumption during heat shock. Syst Synth Biol. 2007;1:47-58 pubmed publisher
    ..The transition from the T to R state is catalyzed by the synergistic action of the substrate and DnaJ cochaperones...
  59. Ohki R, Morita R, Kawamata T, Uchida H, Ohki M. A complete deletion mutant of the Escherichia coli dnaKdnaJ operon. Biochim Biophys Acta. 1989;1009:94-8 pubmed
    Southern hydridization analyses of genomic DNAs from various dnaJ mutants of Escherichia coli showed that mutant K7052, which has well characterized dnaK706 and dnaJ705 double mutantions, is a deletion mutant. The deletion is about 8...
  60. Arsene F, Tomoyasu T, Bukau B. The heat shock response of Escherichia coli. Int J Food Microbiol. 2000;55:3-9 pubmed
    ..Major HSPs are molecular chaperones, including DnaK, DnaJ and GrpE, and GroEL and GroES, and proteases. They constitute the two major chaperone systems of E...
  61. Genevaux P, Georgopoulos C, Kelley W. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol Microbiol. 2007;66:840-57 pubmed
    ..members of both the DnaK (Hsp70) family and their partner J-domain protein (JDP) cochaperone, belonging to the DnaJ (Hsp40) family. Escherichia coli K-12 encodes three Hsp70 genes and six JDP genes...
  62. Bukau B, Horwich A. The Hsp70 and Hsp60 chaperone machines. Cell. 1998;92:351-66 pubmed
  63. Zylicz M, Yamamoto T, McKittrick N, Sell S, Georgopoulos C. Purification and properties of the dnaJ replication protein of Escherichia coli. J Biol Chem. 1985;260:7591-8 pubmed
    The Escherichia coli dnaJ gene was originally discovered because mutations in it blocked bacteriophage lambda DNA replication...
  64. Shirai Y, Akiyama Y, Ito K. Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones. J Bacteriol. 1996;178:1141-5 pubmed
    ..These results suggest that FtsH functions can be somehow compensated for when the cellular concentrations of some molecular chaperones increase. ..
  65. Huang K, Flanagan J, Prestegard J. The influence of C-terminal extension on the structure of the "J-domain" in E. coli DnaJ. Protein Sci. 1999;8:203-14 pubmed
    Two different recombinant constructs of the N-terminal domain in Escherichia coli DnaJ were uniformly labeled with nitrogen-15 and carbon-13...
  66. Cajo G, Horne B, Kelley W, Schwager F, Georgopoulos C, Genevaux P. The role of the DIF motif of the DnaJ (Hsp40) co-chaperone in the regulation of the DnaK (Hsp70) chaperone cycle. J Biol Chem. 2006;281:12436-44 pubmed
    To perform effectively as a molecular chaperone, DnaK (Hsp70) necessitates the assistance of its DnaJ (Hsp40) co-chaperone partner, which efficiently stimulates its intrinsically weak ATPase activity and facilitates its interaction with ..
  67. Zhao X, Braun A, Braun J. Biological roles of neural J proteins. Cell Mol Life Sci. 2008;65:2385-96 pubmed publisher
    ..We are beginning to gain some insights into the neuronal network of J proteins. Here, we highlight recent advances in our understanding of how select J proteins harness Hsp70 s for fundamentally important conformational work in neurons. ..
  68. Itikawa H, Fujita H, Wada M. High temperature induction of a stringent response in the dnaK(Ts) and dnaJ(Ts) mutants of Escherichia coli. J Biochem. 1986;99:1719-24 pubmed
    The cellular concentrations of ppGpp in the dnaK(Ts) and dnaJ(Ts) mutants of Escherichia coli were examined, since the thermosensitive RNA synthesis of these mutants is relaxed by an additional mutation in the relA gene...
  69. Barnett M, Nagy M, Kedzierska S, Zolkiewski M. The amino-terminal domain of ClpB supports binding to strongly aggregated proteins. J Biol Chem. 2005;280:34940-5 pubmed
    ..dehydrogenase (G6PDH) by ClpB and its N-terminally truncated variant ClpBDeltaN in the presence of DnaK, DnaJ, and GrpE...
  70. Tomoyasu T, Ogura T, Tatsuta T, Bukau B. Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli. Mol Microbiol. 1998;30:567-81 pubmed
    ..This hypothesis was tested by altering the modulator concentration in cells expressing dnaK, dnaJ and ftsH from IPTG and arabinose-controlled promoters...
  71. Champ S, Puvirajesinghe T, Perrody E, Menouni R, Genevaux P, Ansaldi M. Chaperone-assisted excisive recombination, a solitary role for DnaJ (Hsp40) chaperone in lysogeny escape. J Biol Chem. 2011;286:38876-85 pubmed publisher
    ..In this work, we identified the host-encoded stress-responsive molecular chaperone DnaJ (Hsp40) as an active participant in KplE1 prophage excision...
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    The effect of mutations in dnaK and dnaJ genes on the expression of two operons that are part of cysteine regulon was determined using Escherichia coli strains harboring cysPTWA::lacZ and cysJIH::lacZ fusions...
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    ..The 44 kDa ATPase fragment does not show biphasic kinetics for ATP binding, and does not show fluorescence changes that suggest conformational changes of the type seen in Hsc70 and the 60 kDa fragment. ..
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    We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA...
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    ..Several heat shock proteins are synthesized in the absence of sigma 32, indicating that there are additional mechanisms controlling the synthesis of some heat shock proteins. ..
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    DnaK-DnaJ-GrpE and GroEL-GroES are the best-characterized molecular chaperone systems in the cytoplasm of Escherichia coli...
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    DnaK, a Hsp70 homolog of Escherichia coli, together with its co-chaperones DnaJ and GrpE protects denatured proteins from aggregation and promotes their refolding by an ATP-consuming mechanism...
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    ..of the sigma(32) subunit of RNA polymerase by reversible association with the DnaK chaperone, mediated by the DnaJ cochaperone. Here we identified two distinct sites in sigma(32) as binding sites for DnaK and DnaJ...
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    ..Our results are consistent with a model in which the N-domain of ClpB95 maximizes substrate processing under conditions where the cellular supply of free DnaK-DnaJ is limiting.
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    We examined the role of two Escherichia coli heat shock proteins, the dnaK and dnaJ gene products, during the initiation of lambda dv DNA replication in vitro...
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    b>DnaJ is a molecular chaperone, which not only binds to its various protein substrates, but can also activate the DnaK cochaperone to bind to its various protein substrates as well...
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    ..The DnaK-DnaJ-GrpE chaperone machinery of V...
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    The DnaK chaperone of Escherichia coli is known to interact with the J domains of DnaJ, CbpA, and DjlA...
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    ..The substrate binding properties of DnaK are controlled by its two cochaperones DnaJ (Hsp40) and GrpE. DnaJ stimulates the hydrolysis of DnaK-bound ATP, and GrpE accelerates ADP/ATP exchange...
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    ..We show here that the sequential action of two Escherichia coli chaperone systems, ClpB and DnaK-DnaJ-GrpE, can efficiently solubilize excess amounts of protein aggregates and refold them into active proteins...