dnaA

Summary

Gene Symbol: dnaA
Description: chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator
Alias: ECK3694, JW3679, hsm-2
Species: Escherichia coli str. K-12 substr. MG1655
Products:     dnaA

Top Publications

  1. Morigen -, Molina F, Skarstad K. Deletion of the datA site does not affect once-per-cell-cycle timing but induces rifampin-resistant replication. J Bacteriol. 2005;187:3913-20 pubmed
    ..First, newly formed origins are inactivated by sequestration; second, the initiator, DnaA, is inactivated by the Hda protein at active replication forks; and third, the level of free DnaA protein is ..
  2. Makise M, Mima S, Koterasawa M, Tsuchiya T, Mizushima T. Biochemical analysis of DnaA protein with mutations in both Arg328 and Lys372. Biochem J. 2002;362:453-8 pubmed
    The DnaA protein is the initiator of chromosomal DNA replication in Escherichia coli. Acidic phospholipids decrease its affinity for adenine nucleotides, and re-activate the ADP-bound form to the ATP-bound form...
  3. Sekimizu K, Bramhill D, Kornberg A. ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome. Cell. 1987;50:259-65 pubmed
    ATP is bound to dnaA protein with high affinity (KD = 0.03 microM) and hydrolyzed slowly to ADP in the presence of DNA. ADP is also bound tightly to dnaA protein and exchanges with ATP very slowly...
  4. Katayama T, Kubota T, Kurokawa K, Crooke E, Sekimizu K. The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E. coli chromosomal replicase. Cell. 1998;94:61-71 pubmed
    The beta subunit of DNA polymerase III is essential for negative regulation of the initiator protein, DnaA. DnaA inactivation occurs through accelerated hydrolysis of ATP bound to DnaA; the resulting ADP-DnaA fails to initiate replication...
  5. Wegrzyn G, Wegrzyn A, Pankiewicz A, Taylor K. Allele specificity of the Escherichia coli dnaA gene function in the replication of plasmids derived from phage lambda. Mol Gen Genet. 1996;252:580-6 pubmed
    We demonstrate a variation in the effects of seven alleles of the Escherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication of lambda phage-derived plasmids at 30 degrees ..
  6. Castuma C, Crooke E, Kornberg A. Fluid membranes with acidic domains activate DnaA, the initiator protein of replication in Escherichia coli. J Biol Chem. 1993;268:24665-8 pubmed
    Acidic phospholipids in a fluid phase dissociate ADP or ATP tightly bound to DnaA protein and, in the presence of ATP and DNA, can restore an inactive ADP form to full activity (Sekimizu, K., and Kornberg, A. (1988) J. Biol. Chem...
  7. Ozaki S, Kawakami H, Nakamura K, Fujikawa N, Kagawa W, Park S, et al. A common mechanism for the ATP-DnaA-dependent formation of open complexes at the replication origin. J Biol Chem. 2008;283:8351-62 pubmed publisher
    ..Escherichia coli DnaA protein forms a homomultimeric complex with the replication origin (oriC)...
  8. Skarstad K, von Meyenburg K, Hansen F, Boye E. Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles. J Bacteriol. 1988;170:852-8 pubmed
    The synchrony of initiation of chromosomal replication in single cells was determined in ten different dnaA(Ts) mutants...
  9. Riber L, Olsson J, Jensen R, Skovgaard O, Dasgupta S, Marinus M, et al. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes Dev. 2006;20:2121-34 pubmed
    Initiation of DNA replication in Eschericia coli requires the ATP-bound form of the DnaA protein...

More Information

Publications88

  1. Fujikawa N, Kurumizaka H, Nureki O, Terada T, Shirouzu M, Katayama T, et al. Structural basis of replication origin recognition by the DnaA protein. Nucleic Acids Res. 2003;31:2077-86 pubmed
    Escherichia coli DnaA binds to 9 bp sequences (DnaA boxes) in the replication origin, oriC, to form a complex initiating chromosomal DNA replication...
  2. Yung B, Kornberg A. Membrane attachment activates dnaA protein, the initiation protein of chromosome replication in Escherichia coli. Proc Natl Acad Sci U S A. 1988;85:7202-5 pubmed
    ADP and ATP are tightly bound to dnaA protein and are crucial to its function in DNA replication; the exchange of these nucleotides is effected specifically by the acidic phospholipids (cardiolipin and phosphatidylglycerol) present in ..
  3. Nozaki S, Yamada Y, Ogawa T. Initiator titration complex formed at datA with the aid of IHF regulates replication timing in Escherichia coli. Genes Cells. 2009;14:329-41 pubmed publisher
    ..controlled by a mechanism referred to as 'initiator titration', a process by which the initiator protein, DnaA, is titrated to newly replicated binding sequences on the chromosome to reduce the initiation potential for ..
  4. Fujimitsu K, Su etsugu M, Yamaguchi Y, Mazda K, Fu N, Kawakami H, et al. Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli. J Bacteriol. 2008;190:5368-81 pubmed publisher
    The chromosomal replication cycle is strictly coordinated with cell cycle progression in Escherichia coli. ATP-DnaA initiates replication, leading to loading of the DNA polymerase III holoenzyme...
  5. Felczak M, Kaguni J. DnaAcos hyperinitiates by circumventing regulatory pathways that control the frequency of initiation in Escherichia coli. Mol Microbiol. 2009;72:1348-63 pubmed publisher
    ..By comparison, an elevated level of DnaA also induces extra initiations, but lethality occurs only in strains defective in repairing double-strand breaks...
  6. Makise M, Mima S, Tsuchiya T, Mizushima T. Molecular mechanism for functional interaction between DnaA protein and acidic phospholipids: identification of important amino acids. J Biol Chem. 2001;276:7450-6 pubmed
    b>DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli, seems to be reactivated from the ADP-bound form to its ATP-bound form through stimulation of ADP release by acidic phospholipids such as cardiolipin...
  7. Marszalek J, Zhang W, Hupp T, Margulies C, Carr K, Cherry S, et al. Domains of DnaA protein involved in interaction with DnaB protein, and in unwinding the Escherichia coli chromosomal origin. J Biol Chem. 1996;271:18535-42 pubmed
    b>DnaA protein of Escherichia coli is a sequence-specific DNA-binding protein required for the initiation of DNA replication from the chromosomal origin, oriC...
  8. Gon S, Camara J, Klungsøyr H, Crooke E, Skarstad K, Beckwith J. A novel regulatory mechanism couples deoxyribonucleotide synthesis and DNA replication in Escherichia coli. EMBO J. 2006;25:1137-47 pubmed
    ..In Escherichia coli, the ATP-bound DnaA protein initiates chromosomal replication...
  9. Ozaki S, Katayama T. DnaA structure, function, and dynamics in the initiation at the chromosomal origin. Plasmid. 2009;62:71-82 pubmed publisher
    Escherichia coli DnaA is the initiator of chromosomal replication...
  10. Marszalek J, Kaguni J. DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli. J Biol Chem. 1994;269:4883-90 pubmed
    b>DnaA protein of Escherichia coli acts in the initiation of chromosomal replication to bind to sequences in the chromosomal origin...
  11. Weigel C, Schmidt A, Seitz H, Tüngler D, Welzeck M, Messer W. The N-terminus promotes oligomerization of the Escherichia coli initiator protein DnaA. Mol Microbiol. 1999;34:53-66 pubmed
    Initiation of chromosome replication in Escherichia coli is governed by the interaction of the initiator protein DnaA with the replication origin oriC...
  12. Yung B, Crooke E, Kornberg A. Fate of the DnaA initiator protein in replication at the origin of the Escherichia coli chromosome in vitro. J Biol Chem. 1990;265:1282-5 pubmed
    The dnaA initiator protein binds specific sequences in the 245-base pair Escherichia coli origin (oriC) to form a series of complexes which eventually are opened enough to admit dnaB helicase into a prepriming complex (Bramhill, D...
  13. Keyamura K, Fujikawa N, Ishida T, Ozaki S, Su etsugu M, Fujimitsu K, et al. The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes. Genes Dev. 2007;21:2083-99 pubmed
    Escherichia coli DiaA is a DnaA-binding protein that is required for the timely initiation of chromosomal replication during the cell cycle. In this study, we determined the crystal structure of DiaA at 1.8 A resolution...
  14. Nyborg M, Atlung T, Skovgaard O, Hansen F. Two types of cold sensitivity associated with the A184-->V change in the DnaA protein. Mol Microbiol. 2000;35:1202-10 pubmed
    Multicopy dnaA(Ts) strains carrying the dnaA5 or dnaA46 allele are high-temperature resistant but are cold sensitive for colony formation...
  15. Kawakami H, Keyamura K, Katayama T. Formation of an ATP-DnaA-specific initiation complex requires DnaA Arginine 285, a conserved motif in the AAA+ protein family. J Biol Chem. 2005;280:27420-30 pubmed
    Escherichia coli DnaA protein, a member of the AAA+ superfamily, initiates replication from the chromosomal origin oriC in an ATP-dependent manner...
  16. Keyamura K, Katayama T. DnaA protein DNA-binding domain binds to Hda protein to promote inter-AAA+ domain interaction involved in regulatory inactivation of DnaA. J Biol Chem. 2011;286:29336-46 pubmed publisher
    ..replication is initiated from the replication origin oriC in Escherichia coli by the active ATP-bound form of DnaA protein...
  17. Wang Q, Kaguni J. Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Mol Gen Genet. 1987;209:518-25 pubmed
    The promoter region of the dnaA gene and of a gene which encodes a 16 kDa protein contain sites which are recognized and bound by dnaA protein...
  18. Sutton M, Kaguni J. Threonine 435 of Escherichia coli DnaA protein confers sequence-specific DNA binding activity. J Biol Chem. 1997;272:23017-24 pubmed
    The Escherichia coli DnaA protein, as a sequence-specific DNA binding protein, promotes the initiation of chromosomal replication by binding to four asymmetric 9-mer sequences termed DnaA boxes in oriC...
  19. Kato J, Katayama T. Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli. EMBO J. 2001;20:4253-62 pubmed publisher
    The bacterial DnaA protein binds to the chromosomal origin of replication to trigger a series of initiation reactions, which leads to the loading of DNA polymerase III...
  20. Ortenberg R, Gon S, Porat A, Beckwith J. Interactions of glutaredoxins, ribonucleotide reductase, and components of the DNA replication system of Escherichia coli. Proc Natl Acad Sci U S A. 2004;101:7439-44 pubmed
    ..of suppressing the growth defects of the multiply mutant strain was by mutations in the DNA replication genes, dnaA and dnaN...
  21. Campbell J, Kleckner N. E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork. Cell. 1990;62:967-79 pubmed
    ..is specifically sequestered from dam methyltransferase for a significant period of time and then released; that the dnaA promoter region is subject to sequestration analogous to that observed at oriC and thus that hemimethylation-..
  22. Sutton M, Kaguni J. Novel alleles of the Escherichia coli dnaA gene. J Mol Biol. 1997;271:693-703 pubmed
    The Escherichia coli dnaA gene is required for replication of the bacterial chromosome...
  23. Simmons L, Felczak M, Kaguni J. DnaA Protein of Escherichia coli: oligomerization at the E. coli chromosomal origin is required for initiation and involves specific N-terminal amino acids. Mol Microbiol. 2003;49:849-58 pubmed
    Iterated DnaA box sequences within the replication origins of bacteria and prokaryotic plasmids are recognized by the replication initiator, DnaA protein. At the E...
  24. Hwang D, Kaguni J. Purification and characterization of the dnaA46 gene product. J Biol Chem. 1988;263:10625-32 pubmed
    ..Facilitated by elevated expression levels, an improved method for purification of dnaA+ protein and the purification of dnaA46 protein have been obtained...
  25. Nievera C, Torgue J, Grimwade J, Leonard A. SeqA blocking of DnaA-oriC interactions ensures staged assembly of the E. coli pre-RC. Mol Cell. 2006;24:581-92 pubmed
    b>DnaA occupies only the three highest-affinity binding sites in E. coli oriC throughout most of the cell cycle...
  26. Makise M, Mima S, Tsuchiya T, Mizushima T. Identification of amino acids involved in the functional interaction between DnaA protein and acidic phospholipids. J Biol Chem. 2000;275:4513-8 pubmed
    b>DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli, seems to be regulated through its binding to acidic phospholipids, such as cardiolipin. In our previous paper (Hase, M., Yoshimi, T., Ishikawa, Y., Ohba, A...
  27. Kitchen J, Li Z, Crooke E. Electrostatic interactions during acidic phospholipid reactivation of DnaA protein, the Escherichia coli initiator of chromosomal replication. Biochemistry. 1999;38:6213-21 pubmed
    The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP...
  28. Hase M, Yoshimi T, Ishikawa Y, Ohba A, Guo L, Mima S, et al. Site-directed mutational analysis for the membrane binding of DnaA protein. Identification of amino acids involved in the functional interaction between DnaA protein and acidic phospholipids. J Biol Chem. 1998;273:28651-6 pubmed
    b>DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli, interacts with acidic phospholipids, such as cardiolipin, and its activity seems to be regulated by membrane binding in cells...
  29. Chodavarapu S, Gomez R, Vicente M, Kaguni J. Escherichia coli Dps interacts with DnaA protein to impede initiation: a model of adaptive mutation. Mol Microbiol. 2008;67:1331-46 pubmed publisher
    ..We report that Dps specifically interacts with DnaA protein by affinity chromatography and a solid phase binding assay, requiring the N-terminal region of DnaA to ..
  30. Nozaki S, Ogawa T. Determination of the minimum domain II size of Escherichia coli DnaA protein essential for cell viability. Microbiology. 2008;154:3379-84 pubmed publisher
    The DnaA protein is the bacterial initiator of replication at a unique chromosomal site, oriC. It is present in all bacterial species and has a conserved structure with four domains...
  31. Speck C, Messer W. Mechanism of origin unwinding: sequential binding of DnaA to double- and single-stranded DNA. EMBO J. 2001;20:1469-76 pubmed
    The initiator protein DnaA of Escherichia coli binds to a 9mer consensus sequence, the DnaA box (5'-TT(A/T)TNCACA). If complexed with ATP it adopts a new binding specificity for a 6mer consensus sequence, the ATP-DnaA box (5'-AGatct)...
  32. Katayama T, Fujimitsu K, Ogawa T. Multiple pathways regulating DnaA function in Escherichia coli: distinct roles for DnaA titration by the datA locus and the regulatory inactivation of DnaA. Biochimie. 2001;83:13-7 pubmed
    Escherichia coli DnaA protein forms a multimeric complex at the chromosomal origin of replication (oriC), where a series of initiation reactions occurs and DNA polymerase III holoenzyme is loaded...
  33. Abe Y, Jo T, Matsuda Y, Matsunaga C, Katayama T, Ueda T. Structure and function of DnaA N-terminal domains: specific sites and mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC. J Biol Chem. 2007;282:17816-27 pubmed
    b>DnaA forms a homomultimeric complex with the origin of chromosomal replication (oriC) to unwind duplex DNA. The interaction of the DnaA N terminus with the DnaB helicase is crucial for the loading of DnaB onto the unwound region...
  34. Simmons L, Kaguni J. The DnaAcos allele of Escherichia coli: hyperactive initiation is caused by substitution of A184V and Y271H, resulting in defective ATP binding and aberrant DNA replication control. Mol Microbiol. 2003;47:755-65 pubmed
    ..Chromosomal DNA replication is regulated at the level of commitment to this biochemical pathway. In Escherichia coli, DnaA protein appears to regulate this process...
  35. Nozaki S, Niki H, Ogawa T. Replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle. J Bacteriol. 2009;191:4807-14 pubmed publisher
    b>DnaA is a replication initiator protein that is conserved among bacteria. It plays a central role in the initiation of DNA replication...
  36. Felczak M, Simmons L, Kaguni J. An essential tryptophan of Escherichia coli DnaA protein functions in oligomerization at the E. coli replication origin. J Biol Chem. 2005;280:24627-33 pubmed
    In the initiation of bacterial DNA replication, DnaA protein recruits DnaB helicase to the chromosomal origin, oriC, leading to the assemble of the replication fork machinery at this site...
  37. Walker J, Severson K, Hermandson M, Blinkova A, Carr K, Kaguni J. Escherichia coli DnaA protein: specific biochemical defects of mutant DnaAs reduce initiation frequency to suppress a temperature-sensitive dnaX mutation. Biochimie. 2006;88:1-10 pubmed
    ..in a domain that functions in ATP binding and the R432L and T435K substitutions affect residues that recognize the DnaA box motif, they might be expected to reduce ATP and specific DNA binding, respectively...
  38. Schaper S, Messer W. Interaction of the initiator protein DnaA of Escherichia coli with its DNA target. J Biol Chem. 1995;270:17622-6 pubmed
    Equilibrium and kinetic rate constants were determined for the binding of the initiator protein DnaA of Escherichia coli to its binding site, the non-palindromic 9-bp DnaA box, using gel retardation techniques...
  39. Hansen E, Hansen F, von Meyenburg K. The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12. Nucleic Acids Res. 1982;10:7373-85 pubmed
    The nucleotide sequence of the dnaA gene and the first 10% of the dnaN gene was determined. From the nucleotide sequence the amino acid sequence of the dnaA gene product was derived...
  40. Roth A, Messer W. High-affinity binding sites for the initiator protein DnaA on the chromosome of Escherichia coli. Mol Microbiol. 1998;28:395-401 pubmed
    The initiator protein DnaA of Escherichia coli binds with unusually high affinity to five regions on the chromosome, in addition to the replication origin, oriC...
  41. Kitagawa R, Ozaki T, Moriya S, Ogawa T. Negative control of replication initiation by a novel chromosomal locus exhibiting exceptional affinity for Escherichia coli DnaA protein. Genes Dev. 1998;12:3032-43 pubmed
    ..7 min on the genetic map that titrates exceptionally large amounts of the bacterial initiator protein, DnaA. A strain lacking datA grew normally but exhibited an asynchronous initiation phenotype as a result of extra ..
  42. Kitagawa R, Mitsuki H, Okazaki T, Ogawa T. A novel DnaA protein-binding site at 94.7 min on the Escherichia coli chromosome. Mol Microbiol. 1996;19:1137-47 pubmed
    There is a DNA sequence which has unusually high affinity for the DnaA protein of Escherichia coli between the glyV and amiB-mutL operons at 94.7 min on the genetic map...
  43. Fang L, Davey M, O Donnell M. Replisome assembly at oriC, the replication origin of E. coli, reveals an explanation for initiation sites outside an origin. Mol Cell. 1999;4:541-53 pubmed
    This study outlines the events downstream of origin unwinding by DnaA, leading to assembly of two replication forks at the E. coli origin, oriC...
  44. Weigel C, Messer W, Preiss S, Welzeck M, Morigen -, Boye E. The sequence requirements for a functional Escherichia coli replication origin are different for the chromosome and a minichromosome. Mol Microbiol. 2001;40:498-507 pubmed
    ..The mutations were subsequently transferred to a wild-type host by transduction. oriC mutants with a mutated DnaA box R1 were not obtained, suggesting that R1 is essential for chromosomal origin function...
  45. Aranovich A, Gdalevsky G, Cohen Luria R, Fishov I, Parola A. Membrane-catalyzed nucleotide exchange on DnaA. Effect of surface molecular crowding. J Biol Chem. 2006;281:12526-34 pubmed
    b>DnaA is the initiator protein for chromosomal replication in bacteria; its activity plays a central role in the timing of the primary initiations within the Escherichia coli cell cycle...
  46. Simmons L, Breier A, Cozzarelli N, Kaguni J. Hyperinitiation of DNA replication in Escherichia coli leads to replication fork collapse and inviability. Mol Microbiol. 2004;51:349-58 pubmed
    Elevated dnaA expression from a multicopy plasmid induces more frequent initiation from the Escherichia coli replication origin, oriC, but viability is maintained...
  47. Katayama T. Feedback controls restrain the initiation of Escherichia coli chromosomal replication. Mol Microbiol. 2001;41:9-17 pubmed
    ..coli, initiation of chromosomal replication is activated by a nucleoprotein complex formed primarily between the DnaA protein and oriC (replication origin) DNA...
  48. Carr K, Kaguni J. Stoichiometry of DnaA and DnaB protein in initiation at the Escherichia coli chromosomal origin. J Biol Chem. 2001;276:44919-25 pubmed
    ..coli chromosomal origin, oriC, occurs through an ordered series of events that depend first on the binding of DnaA protein, the replication initiator, to DnaA box sequences within oriC followed by unwinding of an AT-rich region ..
  49. Felczak M, Kaguni J. The box VII motif of Escherichia coli DnaA protein is required for DnaA oligomerization at the E. coli replication origin. J Biol Chem. 2004;279:51156-62 pubmed
    Escherichia coli DnaA protein initiates DNA replication from the chromosomal origin, oriC, and regulates the frequency of this process. Structure-function studies indicate that the replication initiator comprises four domains...
  50. Hupp T, Kaguni J. Suppression of the Escherichia coli dnaA46 mutation by a mutation in trxA, the gene for thioredoxin. Mol Gen Genet. 1988;213:471-8 pubmed
    ..These results indicate that dasC is allelic with trxA, the gene encoding thioredoxin. ..
  51. Fujimitsu K, Senriuchi T, Katayama T. Specific genomic sequences of E. coli promote replicational initiation by directly reactivating ADP-DnaA. Genes Dev. 2009;23:1221-33 pubmed publisher
    In Escherichia coli, ATP-DnaA, unlike ADP-DnaA, can initiate chromosomal replication at oriC. The level of cellular ATP-DnaA fluctuates, peaking at around the time of replication initiation...
  52. Camara J, Breier A, Brendler T, Austin S, Cozzarelli N, Crooke E. Hda inactivation of DnaA is the predominant mechanism preventing hyperinitiation of Escherichia coli DNA replication. EMBO Rep. 2005;6:736-41 pubmed
    ..Three mechanisms for regulation of replication initiation have been proposed: titration of free DnaA initiator protein by the datA locus, sequestration of newly replicated origins by SeqA protein and regulatory ..
  53. Crooke E, Castuma C, Kornberg A. The chromosome origin of Escherichia coli stabilizes DnaA protein during rejuvenation by phospholipids. J Biol Chem. 1992;267:16779-82 pubmed
    b>DnaA protein (the initiator protein) binds and clusters at the four DnaA boxes of the Escherichia coli chromosomal origin (oriC) to promote the strand opening for DNA replication...
  54. Kimura M, Miki T, Hiraga S, Nagata T, Yura T. Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication. J Bacteriol. 1979;140:825-34 pubmed
    Three amber mutations, dna-801, dna-803, and dna-806, were isolated by localized mutagenesis of the dnaA-oriC region of the chromosome from an Escherichia coli strain carrying temperature-sensitive amber suppressors...
  55. Saxena R, Fingland N, Patil D, Sharma A, Crooke E. Crosstalk between DnaA protein, the initiator of Escherichia coli chromosomal replication, and acidic phospholipids present in bacterial membranes. Int J Mol Sci. 2013;14:8517-37 pubmed publisher
    ..review intriguing in vitro and in vivo evidence that suggest emergent roles for acidic phospholipids in regulating DnaA protein-mediated initiation of Escherichia coli chromosomal replication...
  56. Kasho K, Katayama T. DnaA binding locus datA promotes DnaA-ATP hydrolysis to enable cell cycle-coordinated replication initiation. Proc Natl Acad Sci U S A. 2013;110:936-41 pubmed publisher
    ..To ensure this in Escherichia coli, multiple systems regulate the activity of the replication initiator ATP-DnaA. The level of ATP-DnaA increases before initiation after which it drops via DnaA-ATP hydrolysis, yielding ..
  57. Sekimizu K, Kornberg A. Cardiolipin activation of dnaA protein, the initiation protein of replication in Escherichia coli. J Biol Chem. 1988;263:7131-5 pubmed
    ATP binding to dnaA protein is essential for its action in initiating the replication of plasmids that bear the unique origin of the Escherichia coli chromosome (oriC). ADP bound to that site renders dnaA protein inactive for replication...
  58. Kogoma T, von Meyenburg K. The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12. EMBO J. 1983;2:463-8 pubmed
    ..DNA replication in the absence of protein synthesis (stable DNA replication), tolerate inactivation of the dnaA gene by insertion of transposon Tn10. Furthermore, oriC, the origin of E...
  59. Katayama T, Crooke E. DnaA protein is sensitive to a soluble factor and is specifically inactivated for initiation of in vitro replication of the Escherichia coli minichromosome. J Biol Chem. 1995;270:9265-71 pubmed
    b>DnaA protein loses the capacity to initiate chromosomal replication when treated with a soluble cell extract. This inactivation depends upon DNA and hydrolyzable ribonucleoside triphosphate...
  60. Keyamura K, Abe Y, Higashi M, Ueda T, Katayama T. DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. J Biol Chem. 2009;284:25038-50 pubmed publisher
    ..Escherichia coli ATP-DnaA forms a specific multimer on oriC, resulting in DNA unwinding and DnaB helicase loading...
  61. Hwang D, Kornberg A. Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF. J Biol Chem. 1992;267:23083-6 pubmed
    ..and Kornberg, A. (1988) Cell 54, 915-918). DnaA, the initiator protein, requires protein HU or IHF, along with a millimolar level of ATP and negative superhelical ..
  62. Miller D, Grimwade J, Betteridge T, Rozgaja T, Torgue J, Leonard A. Bacterial origin recognition complexes direct assembly of higher-order DnaA oligomeric structures. Proc Natl Acad Sci U S A. 2009;106:18479-84 pubmed publisher
    ..eubacterium Escherichia coli, there is a temporally similar nucleoprotein complex comprising the initiator protein DnaA bound to three high-affinity recognition sites in the unique origin of replication, oriC...
  63. Makowska Grzyska M, Kaguni J. Primase directs the release of DnaC from DnaB. Mol Cell. 2010;37:90-101 pubmed publisher
    ..The interaction of primase with DnaB and the release of DnaC mark discrete events in the transition from initiation to the elongation stage of DNA replication. ..
  64. Chodavarapu S, Felczak M, Yaniv J, Kaguni J. Escherichia coli DnaA interacts with HU in initiation at the E. coli replication origin. Mol Microbiol. 2008;67:781-92 pubmed publisher
    ..Other results demonstrate that HU stabilizes the DnaA oligomer bound to oriC, and that the alpha subunit of HU interacts with the N-terminal region of DnaA...
  65. Su etsugu M, Shimuta T, Ishida T, Kawakami H, Katayama T. Protein associations in DnaA-ATP hydrolysis mediated by the Hda-replicase clamp complex. J Biol Chem. 2005;280:6528-36 pubmed
    In Escherichia coli, the activity of ATP-bound DnaA protein in initiating chromosomal replication is negatively controlled in a replication-coordinated manner...
  66. Sutton M, Kaguni J. The Escherichia coli dnaA gene: four functional domains. J Mol Biol. 1997;274:546-61 pubmed
    The Escherichia coli DnaA protein is a sequence-specific DNA binding protein that promotes the initiation of replication of the bacterial chromosome, and of several plasmids including pSC101. Twenty-eight novel missense mutations of the E...
  67. Løbner Olesen A, Skarstad K, Hansen F, von Meyenburg K, Boye E. The DnaA protein determines the initiation mass of Escherichia coli K-12. Cell. 1989;57:881-9 pubmed
    DNA replication was studied in a dnaA(Ts) strain containing a plasmid with the dnaA+ gene under plac control...
  68. Aranovich A, Parola A, Fishov I. The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli. FEBS Lett. 2007;581:4439-42 pubmed
    b>DnaA(L366K), in concert with a wild-type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids...
  69. Ishida T, Akimitsu N, Kashioka T, Hatano M, Kubota T, Ogata Y, et al. DiaA, a novel DnaA-binding protein, ensures the timely initiation of Escherichia coli chromosome replication. J Biol Chem. 2004;279:45546-55 pubmed
    The DnaA protein is the initiator of Escherichia coli chromosomal replication. In this study, we identify a novel DnaA-associating protein, DiaA, that is required for the timely initiation of replication during the cell cycle...
  70. Fujimitsu K, Katayama T. Reactivation of DnaA by DNA sequence-specific nucleotide exchange in vitro. Biochem Biophys Res Commun. 2004;322:411-9 pubmed
    In Escherichia coli, ATP-bound DnaA protein can initiate chromosomal replication. After initiation, DnaA-ATP is hydrolyzed by interactions with a complex containing a replicase subunit to yield the inactive ADP-DnaA...
  71. Glinkowska M, Majka J, Messer W, Wegrzyn G. The mechanism of regulation of bacteriophage lambda pR promoter activity by Escherichia coli DnaA protein. J Biol Chem. 2003;278:22250-6 pubmed
    Apart from its function as an initiator of DNA replication, the Escherichia coli DnaA protein is also a specific transcription factor. It activates and represses a number of promoters...
  72. Nishida S, Fujimitsu K, Sekimizu K, Ohmura T, Ueda T, Katayama T. A nucleotide switch in the Escherichia coli DnaA protein initiates chromosomal replication: evidnece from a mutant DnaA protein defective in regulatory ATP hydrolysis in vitro and in vivo. J Biol Chem. 2002;277:14986-95 pubmed
    The ATP-bound DnaA protein opens duplex DNA at the Escherichia coli origin of replication, leading to a series of initiation reactions in vitro...
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