dmsB

Summary

Gene Symbol: dmsB
Description: dimethyl sulfoxide reductase, anaerobic, subunit B
Alias: ECK0886, JW0878
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Cotter P, Gunsalus R. Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli. J Bacteriol. 1989;171:3817-23 pubmed
    ..Additionally, expression of dmsABC varied depending on the carbon source. Expression was highest when cells were grown on sorbitol. ..
  2. Bilous P, Cole S, Anderson W, Weiner J. Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli. Mol Microbiol. 1988;2:785-95 pubmed
    ..The DMS C polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the enzyme to the membrane. ..
  3. Weiner J, MacIsaac D, Bishop R, Bilous P. Purification and properties of Escherichia coli dimethyl sulfoxide reductase, an iron-sulfur molybdoenzyme with broad substrate specificity. J Bacteriol. 1988;170:1505-10 pubmed
    ..The enzyme displayed a very broad substrate specificity, reducing various N-oxide and sulfoxide compounds as well as chlorate and hydroxylamine. ..
  4. Cheng V, Rothery R, Bertero M, Strynadka N, Weiner J. Investigation of the environment surrounding iron-sulfur cluster 4 of Escherichia coli dimethylsulfoxide reductase. Biochemistry. 2005;44:8068-77 pubmed
    ..The electron transfer subunit (DmsB) of Escherichia coli dimethylsulfoxide reductase contains four [4Fe-4S] clusters (FS1-FS4) with E(m) values between ..
  5. Cammack R, Weiner J. Electron paramagnetic resonance spectroscopic characterization of dimethyl sulfoxide reductase of Escherichia coli. Biochemistry. 1990;29:8410-6 pubmed
    ..acid sequence comparisons, that the molybdenum center is located in DmsA, while four iron-sulfur clusters are in DmsB. The midpoint potentials of the molybdenum and iron-sulfur clusters in the various preparations were determined by ..
  6. Tseng C, Hansen A, Cotter P, Gunsalus R. Effect of cell growth rate on expression of the anaerobic respiratory pathway operons frdABCD, dmsABC, and narGHJI of Escherichia coli. J Bacteriol. 1994;176:6599-605 pubmed
    ..The cell appears to have many ways to adjust cell respiration in response to changes in cell growth conditions. ..
  7. Sambasivarao D, Weiner J. Dimethyl sulfoxide reductase of Escherichia coli: an investigation of function and assembly by use of in vivo complementation. J Bacteriol. 1991;173:5935-43 pubmed
    ..The C-terminal region of DmsB appears to interact with the anchor peptide and facilitates the membrane assembly of the catalytic dimer.
  8. Bearson S, Albrecht J, Gunsalus R. Oxygen and nitrate-dependent regulation of dmsABC operon expression in Escherichia coli: sites for Fnr and NarL protein interactions. BMC Microbiol. 2002;2:13 pubmed
    ..The interplay of these transcription factors insures a hierarchical expression of the dmsABC genes when respiration of the preferred electron acceptors, oxygen and nitrate, is not possible. ..
  9. Tseng C, Albrecht J, Gunsalus R. Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coli. J Bacteriol. 1996;178:1094-8 pubmed
    ..These two transcriptional regulators coordinate the hierarchial control of respiratory pathway gene expression in E. coli to ensure the optimal use of oxygen in the cell environment. ..

More Information

Publications19

  1. Trieber C, Rothery R, Weiner J. Multiple pathways of electron transfer in dimethyl sulfoxide reductase of Escherichia coli. J Biol Chem. 1994;269:7103-9 pubmed
    ..were unable to support quinol oxidation although electron transfer from the quinol pool to the [Fe-S] centers in DmsB was normal...
  2. Stanley N, Sargent F, Buchanan G, Shi J, Stewart V, Palmer T, et al. Behaviour of topological marker proteins targeted to the Tat protein transport pathway. Mol Microbiol. 2002;43:1005-21 pubmed
    ..They also suggest that caution should be observed when using subcellular reporter fusions to determine the topological organization of Tat-dependent membrane protein complexes. ..
  3. Bogachev A, Murtazina R, Skulachev V. H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells. J Bacteriol. 1996;178:6233-7 pubmed
    ..These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force. ..
  4. George G, Doonan C, Rothery R, Boroumand N, Weiner J. X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Inorg Chem. 2007;46:2-4 pubmed
    ..Furthermore, our results suggest that the form found in vivo is the monooxobis(molybdopterin) species. ..
  5. Sambasivarao D, Scraba D, Trieber C, Weiner J. Organization of dimethyl sulfoxide reductase in the plasma membrane of Escherichia coli. J Bacteriol. 1990;172:5938-48 pubmed
    ..electron microscopy of everted membrane vesicles and thin sections by using antibodies to the DmsABC, DmsA, DmsB subunits resulted in specific labeling of the cytoplasmic surface of the inner membrane...
  6. Lubitz S, Weiner J. The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC). Arch Biochem Biophys. 2003;418:205-16 pubmed
    ..ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. YnfI (dmsD) has no dms paralogue...
  7. Jack R, Dubini A, Palmer T, Sargent F. Common principles in the biosynthesis of diverse enzymes. Biochem Soc Trans. 2005;33:105-7 pubmed
  8. Rothery R, Weiner J. Alteration of the iron-sulfur cluster composition of Escherichia coli dimethyl sulfoxide reductase by site-directed mutagenesis. Biochemistry. 1991;30:8296-305 pubmed
    ..The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [..
  9. Rothery R, Grant J, Johnson J, Rajagopalan K, Weiner J. Association of molybdopterin guanine dinucleotide with Escherichia coli dimethyl sulfoxide reductase: effect of tungstate and a mob mutation. J Bacteriol. 1995;177:2057-63 pubmed
    ..These data support a model for Mo-MGD biosynthesis and assembly into DmsABC in which both metal chelation and nucleotide addition to MPT precede cofactor insertion. ..
  10. Tang H, Rothery R, Voss J, Weiner J. Correct assembly of iron-sulfur cluster FS0 into Escherichia coli dimethyl sulfoxide reductase (DmsABC) is a prerequisite for molybdenum cofactor insertion. J Biol Chem. 2011;286:15147-54 pubmed publisher
    ..We then combined this change with a DmsA(R61K) mutation and demonstrated that this additional change partially rescued Mo-bisPGD insertion. ..