djlA

Summary

Gene Symbol: djlA
Description: membrane-anchored DnaK co-chaperone, DNA-binding protein
Alias: ECK0056, JW0054, yabH
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Clarke D, Jacq A, Holland I. A novel DnaJ-like protein in Escherichia coli inserts into the cytoplasmic membrane with a type III topology. Mol Microbiol. 1996;20:1273-86 pubmed
    We describe a novel Escherichia coli protein, DjlA, containing a highly conserved J-region motif, which is present in the DnaJ protein chaperone family and required for interaction with DnaK...
  2. Bernard S, Clarke D, Chen M, Holland I, Jacq A. Increased sensitivity of E. coli to novobiocin, EDTA and the anticalmodulin drug W7 following overproduction of DjlA requires a functional transmembrane domain. Mol Gen Genet. 1998;259:645-55 pubmed
    ..We found that this clone in fact suppresses W7 resistance through expression of djlA, which encodes a novel DnaJ-like protein...
  3. Chen M, Takeda S, Yamada H, Ishii Y, Yamashino T, Mizuno T. Characterization of the RcsC-->YojN-->RcsB phosphorelay signaling pathway involved in capsular synthesis in Escherichia coli. Biosci Biotechnol Biochem. 2001;65:2364-7 pubmed
    ..coli. We identified several such genes, namely, rcsB, rcsA, djlA, lolA, and ompG...
  4. Lenski R, Winkworth C, Riley M. Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations. J Mol Evol. 2003;56:498-508 pubmed publisher
    ..However, this limited variation also provides a useful baseline for research aimed at finding the beneficial substitutions in these populations...
  5. Gur E, Katz C, Ron E. All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins. FEBS Lett. 2005;579:1935-9 pubmed
    DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well...
  6. Hennessy F, Nicoll W, Zimmermann R, Cheetham M, Blatch G. Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions. Protein Sci. 2005;14:1697-709 pubmed
    ..We also propose a model in which the J domain is able to integrate specificity and chaperone activity. ..
  7. Castanie Cornet M, Cam K, Jacq A. RcsF is an outer membrane lipoprotein involved in the RcsCDB phosphorelay signaling pathway in Escherichia coli. J Bacteriol. 2006;188:4264-70 pubmed
    ..In contrast, activation of this pathway by overproduction of the membrane chaperone-like protein DjlA does not require RcsF...
  8. Kelley W, Georgopoulos C. Positive control of the two-component RcsC/B signal transduction network by DjlA: a member of the DnaJ family of molecular chaperones in Escherichia coli. Mol Microbiol. 1997;25:913-31 pubmed
  9. Genevaux P, Schwager F, Georgopoulos C, Kelley W. The djlA gene acts synergistically with dnaJ in promoting Escherichia coli growth. J Bacteriol. 2001;183:5747-50 pubmed
    The DnaK chaperone of Escherichia coli is known to interact with the J domains of DnaJ, CbpA, and DjlA. By constructing multiple mutants, we found that the djlA gene was essential for bacterial growth above 37 degrees C in the absence of ..

More Information

Publications16

  1. Toutain C, Clarke D, Leeds J, Kuhn J, Beckwith J, Holland I, et al. The transmembrane domain of the DnaJ-like protein DjlA is a dimerisation domain. Mol Genet Genomics. 2003;268:761-70 pubmed
    b>DjlA is a bitopic inner membrane protein, which belongs to the DnaJ co-chaperone family in Escherichia coli...
  2. Gur E, Biran D, Shechter N, Genevaux P, Georgopoulos C, Ron E. The Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation. J Bacteriol. 2004;186:7236-42 pubmed
    ..Two other E. coli proteins, CbpA and DjlA, which exhibit homology with DnaJ, are known to interact with DnaK and to stimulate its chaperone activity...
  3. Qiu X, Shao Y, Miao S, Wang L. The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. Cell Mol Life Sci. 2006;63:2560-70 pubmed
    ..This review highlights the progress in studying the domains in DnaJ/Hsp40 proteins, introduces the mechanisms by which they interact with Hsp70s, and stresses their functional diversity. ..
  4. Genevaux P, Georgopoulos C, Kelley W. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol Microbiol. 2007;66:840-57 pubmed
    ..In particular, recent advances in structure determination have led to significant insights of the underlying complexities and functional elegance of the Hsp70 chaperone machine. ..
  5. Clarke D, Holland I, Jacq A. Point mutations in the transmembrane domain of DjlA, a membrane-linked DnaJ-like protein, abolish its function in promoting colanic acid production via the Rcs signal transduction pathway. Mol Microbiol. 1997;25:933-44 pubmed
    ..We propose that these mutations are affecting specific interactions between the TMD of DjIA and its substrate protein(s), for example RcsC, the membrane sensor kinase partner of the Rcs/B signal transduction pathway. ..
  6. Genevaux P, Wawrzynow A, Zylicz M, Georgopoulos C, Kelley W. DjlA is a third DnaK co-chaperone of Escherichia coli, and DjlA-mediated induction of colanic acid capsule requires DjlA-DnaK interaction. J Biol Chem. 2001;276:7906-12 pubmed
    b>DjlA is a 30-kDa type III membrane protein of Escherichia coli with the majority, including an extreme C-terminal putative J-domain, oriented toward the cytoplasm...
  7. Shiba Y, Matsumoto K, Hara H. DjlA negatively regulates the Rcs signal transduction system in Escherichia coli. Genes Genet Syst. 2006;81:51-6 pubmed
    ..of Escherichia coli regulating capsular polysaccharide synthesis (cps) genes is activated by overexpression of the djlA gene encoding a cytoplasmic membrane-anchored DnaJ-like protein...