Gene Symbol: dhnA
Description: fructose-bisphosphate aldolase class I
Alias: ECK2090, JW5344, dhnA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Baldwin S, Perham R, Stribling D. Purification and characterization of the class-II D-fructose 1,6-bisphosphate aldolase from Escherichia coli (Crookes' strain). Biochem J. 1978;169:633-41 pubmed
    ..The class-II enzymes from the prokaryote E. coli and the eukaryote yeast show no immunological identity. However, the similarity of their structures suggests that they have evolved from a common ancestor. ..
  2. Lacour S, Landini P. SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences. J Bacteriol. 2004;186:7186-95 pubmed
  3. Alefounder P, Baldwin S, Perham R, Short N. Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli. Biochem J. 1989;257:529-34 pubmed
    ..70% of their soluble protein. This unusually high expression of an E. coli gene should greatly facilitate purification of the enzyme for any future structural or mechanistic studies. ..
  4. Babul J, Fraenkel D. Phosphate modification of fructose-1,6-bisphosphate aldolase in Escherichia coli. Biochem Biophys Res Commun. 1988;151:1033-8 pubmed
    ..Incorporation of label also occurred in the presence of chloramphenicol. Immunoprecipitation revealed apparent aldolase labeling in the wild type strain as well. ..
  5. Stribling D, Perham R. Purification and characterization of two fructose diphosphate aldolases from Escherichia coli (Crookes' strain). Biochem J. 1973;131:833-41 pubmed
    ..Only aldolase of class II was found in E. coli that had been grown on glucose. The significance of these results for the evolution of fructose diphosphate aldolases is briefly discussed. ..
  6. Babul J, Clifton D, Kretschmer M, Fraenkel D. Glucose metabolism in Escherichia coli and the effect of increased amount of aldolase. Biochemistry. 1993;32:4685-92 pubmed
  7. Thomson G, Howlett G, Ashcroft A, Berry A. The dhnA gene of Escherichia coli encodes a class I fructose bisphosphate aldolase. Biochem J. 1998;331 ( Pt 2):437-45 pubmed
    ..coli dehydrin in the GenBanktrade mark database [gene dhnA; entry code U73760; Close and Choi (1996) Submission to GenBanktrade mark]...
  8. Schneider D, Gourse R. Changes in the concentrations of guanosine 5'-diphosphate 3'-diphosphate and the initiating nucleoside triphosphate account for inhibition of rRNA transcription in fructose-1,6-diphosphate aldolase (fda) mutants. J Bacteriol. 2003;185:6192-4 pubmed
  9. Rodina E, Vorobieva N, Kurilova S, Mikulovich J, Vainonen J, Aro E, et al. Identification of new protein complexes of Escherichia coli inorganic pyrophosphatase using pull-down assay. Biochimie. 2011;93:1576-83 pubmed publisher
    ..Comparative analysis of the PPase protein partners allowed an insight into its possible involvement in the cell metabolic regulation. ..

More Information


  1. Baldwin S, Perham R. Novel kinetic and structural properties of the class-I D-fructose 1,6-bisphosphate aldolase from Escherichia coli (Crookes' strain). Biochem J. 1978;169:643-52 pubmed
    ..In view of its unusual properties, it is unlikely that aldolase 1 from E. coli is closely related to the class-1 aldolases that have been detected in several other prokaryotes, or to the typical class-1 enzymes from eukaryotes. ..
  2. Singer M, Walter W, Cali B, Rouviere P, Liebke H, Gourse R, et al. Physiological effects of the fructose-1,6-diphosphate aldolase ts8 mutation on stable RNA synthesis in Escherichia coli. J Bacteriol. 1991;173:6249-57 pubmed
    ..This effect is independent of relA and spoT function. Inhibition is dependent upon glucose metabolism past the generation of glucose-6-phosphate; however, the mechanism of this effect is unknown. ..
  3. Scamuffa M, Caprioli R. Comparison of the mechanisms of two distinct aldolases from Escherichia coli grown on gluconeogenic substrates. Biochim Biophys Acta. 1980;614:583-90 pubmed
    ..The results show that the two enzymes have different mechanisms of action and are consistent with a Schiff-base mechanism for the one which was induced by gluconeogenic substrates and metal-chelate mechanism for the constitutive enzyme. ..
  4. Peng L, Arauzo Bravo M, Shimizu K. Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements. FEMS Microbiol Lett. 2004;235:17-23 pubmed
    ..9% of the carbon flux being channeled via the glyoxylate shunt. However, the flux of the pentose phosphate pathway significantly decreased in the ppc mutant. ..