dgt

Summary

Gene Symbol: dgt
Description: deoxyguanosine triphosphate triphosphohydrolase
Alias: ECK0159, JW0156, optA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Degryse E. Polymorphism in the dgt-dapD-tsf region of Escherichia coli K-12 strains. Gene. 1991;102:141-2 pubmed
    ..This RFLP, which includes the BamHI, EcoRI and SalI sites, may be useful in classification of various E. coli strains. ..
  2. Quirk S, Bhatnagar S, Bessman M. Primary structure of the deoxyguanosine triphosphate triphosphohydrolase-encoding gene (dgt) of Escherichia coli. Gene. 1990;89:13-8 pubmed
    The complete nucleotide sequence has been determined for a 2027-bp region that encompasses the structural gene (dgt) encoding deoxyguanosine triphosphate triphosphohydrolase (dGTPase) from Escherichia coli...
  3. Wurgler S, Richardson C. Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli. Proc Natl Acad Sci U S A. 1990;87:2740-4 pubmed
    ..6 min on the E. coli chromosome and is closely linked to dapD. We have isolated the gene encoding dGTPase (dgt) from wild-type E. coli and determined its nucleotide sequence...
  4. Seto D, Bhatnagar S, Bessman M. The purification and properties of deoxyguanosine triphosphate triphosphohydrolase from Escherichia coli. J Biol Chem. 1988;263:1494-9 pubmed
    ..Preliminary evidence suggest that this enzyme is responsible for the optA mutant phenotype first described by Saito and Richardson (Saito, H., and Richardson, C.C. (1981) J. Virol...
  5. Beauchamp B, Richardson C. A unique deoxyguanosine triphosphatase is responsible for the optA1 phenotype of Escherichia coli. Proc Natl Acad Sci U S A. 1988;85:2563-7 pubmed
    ..Extracts of E. coli optA1 catalyze the hydrolysis of dGTP at a rate 50-fold greater than do extracts of E. coli optA+. The dGTPase responsible for the increased hydrolysis has been purified to apparent homogeneity...
  6. Wurgler S, Richardson C. DNA binding properties of the deoxyguanosine triphosphate triphosphohydrolase of Escherichia coli. J Biol Chem. 1993;268:20046-54 pubmed
    The dgt gene of Escherichia coli encodes a deoxyguanosine triphosphate triphosphohydrolase (dGTPase) that hydrolyzes dGTP to deoxyguanosine and tripolyphosphate...
  7. KORNBERG S, Lehman I, Bessman M, Simms E, Kornberg A. Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate. J Biol Chem. 1958;233:159-62 pubmed
  8. Gawel D, Hamilton M, Schaaper R. A novel mutator of Escherichia coli carrying a defect in the dgt gene, encoding a dGTP triphosphohydrolase. J Bacteriol. 2008;190:6931-9 pubmed publisher
    ..Several mutators in this collection were found to have an insertion in the dgt gene, encoding a previously characterized dGTP triphosphohydrolase...
  9. Quirk S, Bessman M. dGTP triphosphohydrolase, a unique enzyme confined to members of the family Enterobacteriaceae. J Bacteriol. 1991;173:6665-9 pubmed
    ..Mol. Biol. 193:579-584, 1987) and may account for the enzyme's observed interaction with DNA. As far as we are aware, this is the only enzymatic activity so far reported to be present solely in the enteric bacteria...

More Information

Publications14

  1. Nakai H, Richardson C. The gene 1.2 protein of bacteriophage T7 interacts with the Escherichia coli dGTP triphosphohydrolase to form a GTP-binding protein. J Biol Chem. 1990;265:4411-9 pubmed
    ..dGTP is most effective in preventing the association of the enzyme with the inhibitor whereas GTP is most effective in preventing the dissociation of the enzyme-inhibitor complex. ..
  2. Quirk S, Seto D, Bhatnagar S, Gauss P, Gold L, Bessman M. Location and molecular cloning of the structural gene for the deoxyguanosine triphosphate triphosphohydrolase of Escherichia coli. Mol Microbiol. 1989;3:1391-5 pubmed
    The structural gene for deoxyguanosine triphosphate triphosphohydrolase (dGTPase) (EC 3.1.5.1) and its regulator, optA, have been located on a lambda phage carrying a 17.5kb Escherichia coli DNA insert...
  3. Kim Y, Lew C, Gralla J. Escherichia coli pfs transcription: regulation and proposed roles in autoinducer-2 synthesis and purine excretion. J Bacteriol. 2006;188:7457-63 pubmed
    ..The negative element is contained within the overlapping dgt promoter, which is involved in purine metabolism...
  4. Itsko M, Schaaper R. The dgt gene of Escherichia coli facilitates thymine utilization in thymine-requiring strains. Mol Microbiol. 2011;81:1221-32 pubmed publisher
    The Escherichia coli dGTP triphosphohydrolase (dGTPase) encoded by the dgt gene catalyses the hydrolysis of dGTP to deoxyguanosine and triphosphate...
  5. Singh D, Gawel D, Itsko M, Hochkoeppler A, Krahn J, London R, et al. Structure of Escherichia coli dGTP triphosphohydrolase: a hexameric enzyme with DNA effector molecules. J Biol Chem. 2015;290:10418-29 pubmed publisher
    The Escherichia coli dgt gene encodes a dGTP triphosphohydrolase whose detailed role still remains to be determined...