degP

Summary

Gene Symbol: degP
Description: serine endoprotease (protease Do), membrane-associated
Alias: ECK0160, JW0157, htrA, ptd
Species: Escherichia coli str. K-12 substr. MG1655
Products:     degP

Top Publications

  1. Spiers A, Lamb H, Cocklin S, Wheeler K, Budworth J, Dodds A, et al. PDZ domains facilitate binding of high temperature requirement protease A (HtrA) and tail-specific protease (Tsp) to heterologous substrates through recognition of the small stable RNA A (ssrA)-encoded peptide. J Biol Chem. 2002;277:39443-9 pubmed
    The Escherichia coli protease HtrA has two PDZ domains, and sequence alignments predict that the E. coli protease Tsp has a single PDZ domain...
  2. Isaac D, Pinkner J, Hultgren S, Silhavy T. The extracytoplasmic adaptor protein CpxP is degraded with substrate by DegP. Proc Natl Acad Sci U S A. 2005;102:17775-9 pubmed
    ..coli and that mutations in either cpxP or the gene for the periplasmic protease DegP prevent suppression by preventing the degradation of these proteins...
  3. Spiess C, Beil A, Ehrmann M. A temperature-dependent switch from chaperone to protease in a widely conserved heat shock protein. Cell. 1999;97:339-47 pubmed
    ..We show here that the widely conserved heat shock protein DegP (HtrA) has both general molecular chaperone and proteolytic activities...
  4. Lipinska B, Zylicz M, Georgopoulos C. The HtrA (DegP) protein, essential for Escherichia coli survival at high temperatures, is an endopeptidase. J Bacteriol. 1990;172:1791-7 pubmed
    As a preliminary step in the understanding of the function of the Escherichia coli HtrA (DegP) protein, which is indispensable for bacterial survival only at elevated temperatures, the protein was purified and partially characterized...
  5. Skorko Glonek J, Zurawa D, Tanfani F, Scire A, Wawrzynów A, Narkiewicz J, et al. The N-terminal region of HtrA heat shock protease from Escherichia coli is essential for stabilization of HtrA primary structure and maintaining of its oligomeric structure. Biochim Biophys Acta. 2003;1649:171-82 pubmed
    b>HtrA heat shock protease is highly conserved in evolution, and in Escherichia coli, it protects the cell by degradation of proteins denatured by heat and oxidative stress, and also degrades misfolded proteins with reduced disulfide bonds...
  6. Misra R, Peterson A, Ferenci T, Silhavy T. A genetic approach for analyzing the pathway of LamB assembly into the outer membrane of Escherichia coli. J Biol Chem. 1991;266:13592-7 pubmed
    ..These monomers are rapidly degraded in the wild type (htrA+) strain, and the biphasic kinetics of this degradation indicate that the mutation affects the assembly process and ..
  7. Pogliano J, Lynch A, Belin D, Lin E, Beckwith J. Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system. Genes Dev. 1997;11:1169-82 pubmed
    ..of the Cpx pathway induces 5- to 10-fold the synthesis of DsbA, required for disulfide bond formation, and DegP, a major periplasmic protease...
  8. Kim S, Grant R, Sauer R. Covalent linkage of distinct substrate degrons controls assembly and disassembly of DegP proteolytic cages. Cell. 2011;145:67-78 pubmed publisher
    Protein quality control requires careful regulation of intracellular proteolysis. For DegP, a periplasmic protease, substrates promote assembly of inactive hexamers into proteolytically active cages with 12, 18, 24, or 30 subunits...
  9. Skorko Glonek J, Wawrzynow A, Krzewski K, Kurpierz K, Lipinska B. Site-directed mutagenesis of the HtrA (DegP) serine protease, whose proteolytic activity is indispensable for Escherichia coli survival at elevated temperatures. Gene. 1995;163:47-52 pubmed
    The HtrA(DegP) 48-kDa serine protease of Escherichia coli is indispensable for bacterial survival at elevated temperatures...

More Information

Publications75

  1. Krojer T, Garrido Franco M, Huber R, Ehrmann M, Clausen T. Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine. Nature. 2002;416:455-9 pubmed
    ..To investigate the molecular basis of this process, we have solved the crystal structure of DegP (also known as HtrA), a widely conserved heat shock protein that combines refolding and proteolytic activities...
  2. Strozen T, Langen G, Howard S. Adenylate cyclase mutations rescue the degP temperature-sensitive phenotype and induce the sigma E and Cpx extracytoplasmic stress regulons in Escherichia coli. J Bacteriol. 2005;187:6309-16 pubmed
    Inactivation of the gene encoding the periplasmic protease DegP confers a high-temperature-sensitive phenotype in Escherichia coli. We have previously demonstrated that a degP mutant of E...
  3. Skorko Glonek J, Sobiecka Szkatuła A, Lipinska B. Characterization of disulfide exchange between DsbA and HtrA proteins from Escherichia coli. Acta Biochim Pol. 2006;53:585-9 pubmed
    ..work we provided the first detailed characterization of disulfide exchange between DsbA and its natural substrate, HtrA protease. We demonstrated that HtrA oxidation relies on DsbA, both in vivo and in vitro...
  4. Gerken H, Charlson E, Cicirelli E, Kenney L, Misra R. MzrA: a novel modulator of the EnvZ/OmpR two-component regulon. Mol Microbiol. 2009;72:1408-22 pubmed publisher
    ..Furthermore, our data show that MzrA links the two-component envelope stress response regulators, CpxA/CpxR and EnvZ/OmpR. ..
  5. Jiang J, Zhang X, Chen Y, Wu Y, Zhou Z, Chang Z, et al. Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins. Proc Natl Acad Sci U S A. 2008;105:11939-44 pubmed publisher
    Cells use molecular chaperones and proteases to implement the essential quality control mechanism of proteins. The DegP (HtrA) protein, essential for the survival of Escherichia coli cells at elevated temperatures with homologues found in ..
  6. Rizzitello A, Harper J, Silhavy T. Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. J Bacteriol. 2001;183:6794-800 pubmed publisher
    ..Using depletion analysis, we directly demonstrated that null mutations in skp and surA, as well as in degP and surA, result in synthetic phenotypes, suggesting that Skp, SurA, and DegP are functionally redundant...
  7. Sobiecka Szkatula A, Polit A, Scire A, Gieldon A, Tanfani F, Szkarlat Z, et al. Temperature-induced conformational changes within the regulatory loops L1-L2-LA of the HtrA heat-shock protease from Escherichia coli. Biochim Biophys Acta. 2009;1794:1573-82 pubmed publisher
    The present investigation was undertaken to characterize mechanism of thermal activation of serine protease HtrA (DegP) from Escherichia coli...
  8. Krojer T, Sawa J, Huber R, Clausen T. HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues. Nat Struct Mol Biol. 2010;17:844-52 pubmed publisher
    ..To address this, we carried out biochemical and structural studies of DegP from Escherichia coli...
  9. Misra R, CastilloKeller M, Deng M. Overexpression of protease-deficient DegP(S210A) rescues the lethal phenotype of Escherichia coli OmpF assembly mutants in a degP background. J Bacteriol. 2000;182:4882-8 pubmed
    ..Synthesis of all mutant OmpF proteins elevated the expression of periplasmic protease DegP, and synthesis of most of them made its presence obligatory for cell viability...
  10. Strauch K, Johnson K, Beckwith J. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. J Bacteriol. 1989;171:2689-96 pubmed
    The degP gene, required for proteolysis in the cell envelope of Escherichia coli, maps at approximately 3.5 min on the chromosome. Null mutations in degP result in temperature-sensitive growth...
  11. Lipinska B, Fayet O, Baird L, Georgopoulos C. Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures. J Bacteriol. 1989;171:1574-84 pubmed
    We identified and cloned an Escherichia coli gene called htrA (high temperature requirement). The htrA gene was originally discovered because mini-Tn10 transposon insertions in it allowed E...
  12. Skorko Glonek J, Lipinska B, Krzewski K, Zolese G, Bertoli E, Tanfani F. HtrA heat shock protease interacts with phospholipid membranes and undergoes conformational changes. J Biol Chem. 1997;272:8974-82 pubmed
    The HtrA (DegP) protein of Escherichia coli is a heat shock serine protease, essential for cell survival only at temperatures above 42 degrees C...
  13. Sklar J, Wu T, Kahne D, Silhavy T. Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Genes Dev. 2007;21:2473-84 pubmed
    ..through an unknown mechanism that requires the outer membrane YaeT complex and the periplasmic chaperones SurA, DegP, and Skp...
  14. Skorko Glonek J, Zurawa D, Kuczwara E, Wozniak M, Wypych Z, Lipinska B. The Escherichia coli heat shock protease HtrA participates in defense against oxidative stress. Mol Gen Genet. 1999;262:342-50 pubmed
    The serine protease HtrA (DegP), which is indispensable for cell survival at elevated temperatures, is a peripheral membrane protein, localized on the periplasmic side of the inner membrane in Escherichia coli, and the biochemical and ..
  15. Shen Q, Bai X, Chang L, Wu Y, Wang H, Sui S. Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control. Proc Natl Acad Sci U S A. 2009;106:4858-63 pubmed publisher
    In the periplasm of Escherichia coli, DegP (also known as HtrA), which has both chaperone-like and proteolytic activities, prevents the accumulation of toxic misfolded and unfolded polypeptides...
  16. Krojer T, Sawa J, Schäfer E, Saibil H, Ehrmann M, Clausen T. Structural basis for the regulated protease and chaperone function of DegP. Nature. 2008;453:885-90 pubmed publisher
    All organisms have to monitor the folding state of cellular proteins precisely. The heat-shock protein DegP is a protein quality control factor in the bacterial envelope that is involved in eliminating misfolded proteins and in the ..
  17. Skorko Glonek J, Sobiecka Szkatula A, Narkiewicz J, Lipinska B. The proteolytic activity of the HtrA (DegP) protein from Escherichia coli at low temperatures. Microbiology. 2008;154:3649-58 pubmed publisher
    The HtrA (DegP) protein from Escherichia coli is a periplasmic protease whose function is to protect cells from the deleterious effects of various stress conditions...
  18. Skorko Glonek J, Krzewski K, Lipinska B, Bertoli E, Tanfani F. Comparison of the structure of wild-type HtrA heat shock protease and mutant HtrA proteins. A Fourier transform infrared spectroscopic study. J Biol Chem. 1995;270:11140-6 pubmed
    The HtrA protease of Escherichia coli, identical with the DegP protease, is a 48-kDa heat shock protein, indispensable for bacterial survival only at temperatures above 42 degrees C...
  19. DiGiuseppe P, Silhavy T. Signal detection and target gene induction by the CpxRA two-component system. J Bacteriol. 2003;185:2432-40 pubmed
    ..Finally, we show that two Cpx-regulated genes are differentially upregulated in response to different envelope stresses, suggesting the existence of three stress-responsive systems. ..
  20. CastilloKeller M, Misra R. Protease-deficient DegP suppresses lethal effects of a mutant OmpC protein by its capture. J Bacteriol. 2003;185:148-54 pubmed
    ..due to the presence of two non-native cysteine residues, is lethal in cells lacking the major periplasmic protease, DegP. This lethality is not observed in dsbA strains that have diminished ability to form periplasmic disulfide bonds...
  21. Lipinska B, Sharma S, Georgopoulos C. Sequence analysis and regulation of the htrA gene of Escherichia coli: a sigma 32-independent mechanism of heat-inducible transcription. Nucleic Acids Res. 1988;16:10053-67 pubmed
    Previous work has established that the E. coli htrA gene product is essential for bacterial survival at temperatures above 42 degrees...
  22. Kadokura H, Kawasaki H, Yoda K, Yamasaki M, Kitamoto K. Efficient export of alkaline phosphatase overexpressed from a multicopy plasmid requires degP, a gene encoding a periplasmic protease of Escherichia coli. J Gen Appl Microbiol. 2001;47:133-141 pubmed
    Escherichia coli DegP is an inducible serine protease which is involved in the breakdown of abberant proteins arising in the periplasmic compartment. Overexpression of alkaline phosphatase (PhoA) increased transcription of degP by twofold...
  23. Clausen T, Southan C, Ehrmann M. The HtrA family of proteases: implications for protein composition and cell fate. Mol Cell. 2002;10:443-55 pubmed
    ..One quality control factor is HtrA, one of a new class of oligomeric serine proteases...
  24. Jones C, Dexter P, Evans A, Liu C, Hultgren S, Hruby D. Escherichia coli DegP protease cleaves between paired hydrophobic residues in a natural substrate: the PapA pilin. J Bacteriol. 2002;184:5762-71 pubmed
    The DegP protein, a multifunctional chaperone and protease, is essential for clearance of denatured or aggregated proteins from the inner-membrane and periplasmic space in Escherichia coli...
  25. Cavard D. Role of DegP protease on levels of various forms of colicin A lysis protein. FEMS Microbiol Lett. 1995;125:173-8 pubmed
    ..It depended mainly on the presence in the producing strain of a degP gene which encodes the DegP protease...
  26. Sobiecka Szkatula A, Gieldon A, Scire A, Tanfani F, Figaj D, Koper T, et al. The role of the L2 loop in the regulation and maintaining the proteolytic activity of HtrA (DegP) protein from Escherichia coli. Arch Biochem Biophys. 2010;500:123-30 pubmed publisher
    ..particular elements of the regulatory loop L2 in the activation process and maintaining the proteolytic activity of HtrA (DegP) from Escherichia coli...
  27. Gatsos X, Perry A, Anwari K, Dolezal P, Wolynec P, Likic V, et al. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiol Rev. 2008;32:995-1009 pubmed publisher
  28. Ortega J, Iwanczyk J, Jomaa A. Escherichia coli DegP: a structure-driven functional model. J Bacteriol. 2009;191:4705-13 pubmed publisher
  29. Wurgler S, Richardson C. Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli. Proc Natl Acad Sci U S A. 1990;87:2740-4 pubmed
    ..coli and determined its nucleotide sequence. The dgt gene lies immediately upstream of htrA and 6 kilobases from dapD, in the same region as the optA1 mutation...
  30. Pallen M, Wren B. The HtrA family of serine proteases. Mol Microbiol. 1997;26:209-21 pubmed
    HtrA, also known as DegP and probably identical to the Do protease, is a heat shock-induced serine protease that is active in the periplasm of Escherichia coli...
  31. Soltes G, Martin N, Park E, Sutterlin H, Silhavy T. Distinctive Roles for Periplasmic Proteases in the Maintenance of Essential Outer Membrane Protein Assembly. J Bacteriol. 2017;199: pubmed publisher
    ..mutants of LptD, the essential lipopolysaccharide assembly translocon, to show that the periplasmic protease DegP degrades substrates with assembly defects that prevent or impair initial contact with Bam, causing the mutant ..
  32. Lyu Z, Zhao X. Periplasmic quality control in biogenesis of outer membrane proteins. Biochem Soc Trans. 2015;43:133-8 pubmed publisher
    ..In Escherichia coli, Skp, SurA and DegP are the most prominent factors identified to guide OMPs across the periplasm and to play the role of quality ..
  33. Danese P, Oliver G, Barr K, Bowman G, Rick P, Silhavy T. Accumulation of the enterobacterial common antigen lipid II biosynthetic intermediate stimulates degP transcription in Escherichia coli. J Bacteriol. 1998;180:5875-84 pubmed
    In Escherichia coli, transcription of the degP locus, which encodes a heat-shock-inducible periplasmic protease, is controlled by two parallel signal transduction systems that each monitor extracytoplasmic protein physiology...
  34. Huber D, Bukau B. DegP: a Protein "Death Star". Structure. 2008;16:989-90 pubmed publisher
  35. Kihara A, Ito K. Translocation, folding, and stability of the HflKC complex with signal anchor topogenic sequences. J Biol Chem. 1998;273:29770-5 pubmed
    ..Singly expressed HflK and HflC were unstable in vivo and periplasmic proteases DegP and Prc were involved in the degradation of the HflK subunit...
  36. Werner J, Misra R. YaeT (Omp85) affects the assembly of lipid-dependent and lipid-independent outer membrane proteins of Escherichia coli. Mol Microbiol. 2005;57:1450-9 pubmed
    ..Our data suggest that YaeT's role in the assembly of OMPs is not mediated through a role in lipid biogenesis, as debated for Omp85 in Neisseria, thus advocating a conserved OMP assembly function of Omp85 homologues. ..
  37. Miyadai H, Tanaka Masuda K, Matsuyama S, Tokuda H. Effects of lipoprotein overproduction on the induction of DegP (HtrA) involved in quality control in the Escherichia coli periplasm. J Biol Chem. 2004;279:39807-13 pubmed
    ..previously identified lipoproteins, only an outer membrane lipoprotein, NlpE, is known to induce expression of the degP gene upon its overproduction...
  38. Sassoon N, Arié J, Betton J. PDZ domains determine the native oligomeric structure of the DegP (HtrA) protease. Mol Microbiol. 1999;33:583-9 pubmed
    b>DegP (HtrA) is a periplasmic heat shock serine protease of Escherichia coli that degrades misfolded proteins at high temperatures. Biochemical and biophysical experiments have indicated that the purified DegP exists as a hexamer...
  39. Kolmar H, Waller P, Sauer R. The DegP and DegQ periplasmic endoproteases of Escherichia coli: specificity for cleavage sites and substrate conformation. J Bacteriol. 1996;178:5925-9 pubmed
    b>DegP and DegQ are homologous endoproteases found in the periplasmic compartment of Escherichia coli...
  40. Leiser O, Charlson E, Gerken H, Misra R. Reversal of the ?degP phenotypes by a novel rpoE allele of Escherichia coli. PLoS ONE. 2012;7:e33979 pubmed publisher
    ..Revertants of a ?degP ?bamB strain, which fails to grow at 37°C due to high envelope stress, harbored mutations in the rseA and rpoE ..
  41. Meltzer M, Hasenbein S, Mamant N, Merdanovic M, Poepsel S, Hauske P, et al. Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli. Res Microbiol. 2009;160:660-6 pubmed publisher
    Two members of the widely conserved HtrA family of serine proteases, DegP and DegS, are key players in extracytoplasmic protein quality control...
  42. Seol J, Woo S, Jung E, Yoo S, Lee C, Kim K, et al. Protease Do is essential for survival of Escherichia coli at high temperatures: its identity with the htrA gene product. Biochem Biophys Res Commun. 1991;176:730-6 pubmed
    ..Moreover, they were unable to survive at high temperatures, similarly to the htrA mutants...
  43. Tran T, Struck D, Young R. The T4 RI antiholin has an N-terminal signal anchor release domain that targets it for degradation by DegP. J Bacteriol. 2007;189:7618-25 pubmed
    ..Moreover, the SAR domain confers both functional lability and DegP-mediated proteolytic instability on the released form of RI, although LIN is not affected in a degP host...
  44. Quirk S, Bhatnagar S, Bessman M. Primary structure of the deoxyguanosine triphosphate triphosphohydrolase-encoding gene (dgt) of Escherichia coli. Gene. 1990;89:13-8 pubmed
    ..The gene resides between the htrA and dapD loci at 3.75-3.8' on the bacterial chromosome...
  45. Day C, Hinds M. HtrA--a renaissance protein. Structure. 2002;10:737-9 pubmed
    Initially identified as proteases, members of the HtrA/DegP family of proteins have also been shown to act as chaperones in bacteria, and more recently implicated, as regulators of apoptosis in mammals...
  46. Forns N, Juárez A, Madrid C. Osmoregulation of the HtrA (DegP) protease of Escherichia coli: an Hha-H-NS complex represses HtrA expression at low osmolarity. FEMS Microbiol Lett. 2005;251:75-80 pubmed
    The HtrA protein of Escherichia coli is a heat-shock inducible periplasmic protease, essential for bacterial survival at high temperatures...
  47. Iwanczyk J, Damjanovic D, Kooistra J, Leong V, Jomaa A, Ghirlando R, et al. Role of the PDZ domains in Escherichia coli DegP protein. J Bacteriol. 2007;189:3176-86 pubmed
    ..The Escherichia coli essential periplasmic protein DegP contains two PDZ domains attached to the C-terminal end of the protease domain...
  48. Swamy K, Chung C, Goldberg A. Isolation and characterization of protease do from Escherichia coli, a large serine protease containing multiple subunits. Arch Biochem Biophys. 1983;224:543-54 pubmed
    ..When cells growing in rich broth enter stationary phase, the relative concentration of protease Do increases more than twofold. ..
  49. Hiratsu K, Amemura M, Nashimoto H, Shinagawa H, Makino K. The rpoE gene of Escherichia coli, which encodes sigma E, is essential for bacterial growth at high temperature. J Bacteriol. 1995;177:2918-22 pubmed
    ..containing an alternative sigma subunit, sigma E, activates transcription from one of the rpoH promoters and the htrA promoter...
  50. Subrini O, Betton J. Assemblies of DegP underlie its dual chaperone and protease function. FEMS Microbiol Lett. 2009;296:143-8 pubmed publisher
    ..Recent structural studies showed how DegP, a periplasmic heat-shock protease of Escherichia coli, assembles into large homooligomers with an internal cavity ..
  51. Windbichler N, von Pelchrzim F, Mayer O, Csaszar E, Schroeder R. Isolation of small RNA-binding proteins from E. coli: evidence for frequent interaction of RNAs with RNA polymerase. RNA Biol. 2008;5:30-40 pubmed
    ..Our observations show that RNA polymerase is able to bind and react with many different RNAs and we suggest that RNAs are involved in transcriptional regulation more frequently than anticipated. ..
  52. Zurawa Janicka D, Narkiewicz J, Lipinska B. [Characterization of the HtrA family of proteins]. Postepy Biochem. 2007;53:27-36 pubmed
    The HtrA family of proteins consists of evolutionary conserved serine proteases, which are homologues of the HtrA protein from a model bacterium Escherichia coli...
  53. Buelow D, Raivio T. Cpx signal transduction is influenced by a conserved N-terminal domain in the novel inhibitor CpxP and the periplasmic protease DegP. J Bacteriol. 2005;187:6622-30 pubmed
    ..of this study, we also found that CpxP'-'Bla stability is differentially controlled by the periplasmic protease DegP in response to inducing cues and that mutation of degP diminishes Cpx pathway activity...
  54. Matsuyama S, Tokuda H. [Transport of outer membrane proteins]. Tanpakushitsu Kakusan Koso. 2004;49:965-6 pubmed
  55. Ting H, Kouzminova E, Kuzminov A. Synthetic lethality with the dut defect in Escherichia coli reveals layers of DNA damage of increasing complexity due to uracil incorporation. J Bacteriol. 2008;190:5841-54 pubmed publisher
    ..In addition, two SL(dut) inserts (phoU and degP) identify functions that could act to support the weakened activity of the Dut-1 mutant enzyme, suggesting the &..
  56. Langen G, Harper J, Silhavy T, Howard S. Absence of the outer membrane phospholipase A suppresses the temperature-sensitive phenotype of Escherichia coli degP mutants and induces the Cpx and sigma(E) extracytoplasmic stress responses. J Bacteriol. 2001;183:5230-8 pubmed
    b>DegP is a periplasmic protease that is a member of both the sigma(E) and Cpx extracytoplasmic stress regulons of Escherichia coli and is essential for viability at temperatures above 42 degrees C...
  57. Cavard D, Lazdunski C, Howard S. The acylated precursor form of the colicin A lysis protein is a natural substrate of the DegP protease. J Bacteriol. 1989;171:6316-22 pubmed
    The acylated precursor form of the colicin A lysis protein (pCalm) is specifically cleaved by the DegP protease into two acylated fragments of 6 and 4.5 kilodaltons (kDa)...
  58. Gill R, Valdes J, Bentley W. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metab Eng. 2000;2:178-89 pubmed
    ..Specifically, the responses included significantly increased upregulation of heat shock (ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and bacteriophage ..
  59. Ulvatne H, Haukland H, Samuelsen Ø, Kramer M, Vorland L. Proteases in Escherichia coli and Staphylococcus aureus confer reduced susceptibility to lactoferricin B. J Antimicrob Chemother. 2002;50:461-7 pubmed
    ..coli and S. aureus. We also report that genetic inactivation of the heat shock-induced serine protease DegP increased the susceptibility to lactoferricin B in E...
  60. McBroom A, Johnson A, Vemulapalli S, Kuehn M. Outer membrane vesicle production by Escherichia coli is independent of membrane instability. J Bacteriol. 2006;188:5385-92 pubmed
    ..Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesiculation levels, demonstrating that vesicle production is not predictive of envelope instability. ..
  61. Jomaa A, Damjanovic D, Leong V, Ghirlando R, Iwanczyk J, Ortega J. The inner cavity of Escherichia coli DegP protein is not essential for molecular chaperone and proteolytic activity. J Bacteriol. 2007;189:706-16 pubmed
    The Escherichia coli DegP protein is an essential periplasmic protein for bacterial survival at high temperatures...
  62. Gao T, O Brian M. Control of DegP-dependent degradation of c-type cytochromes by heme and the cytochrome c maturation system in Escherichia coli. J Bacteriol. 2007;189:6253-9 pubmed
    ..These levels were restored in a degP mutant strain, showing that apocytochrome c550 is degraded by the periplasmic protease DegP...
  63. Andersen C, Holland I, Jacq A. Verapamil, a Ca2+ channel inhibitor acts as a local anesthetic and induces the sigma E dependent extra-cytoplasmic stress response in E. coli. Biochim Biophys Acta. 2006;1758:1587-95 pubmed
    ..This is accompanied by induction of degP expression, as a result of the activation of the RpoE (SigmaE) extra-cytoplasmic stress response, and activation of ..
  64. Kim K, Park S, Kang S, Cheong G, Chung C. Selective degradation of unfolded proteins by the self-compartmentalizing HtrA protease, a periplasmic heat shock protein in Escherichia coli. J Mol Biol. 1999;294:1363-74 pubmed
    b>HtrA, which has a high molecular mass of about 500 kDa, is a periplasmic heat shock protein whose proteolytic activity is essential for the survival of Escherichia coli at high temperatures...
  65. Cavard D. Inhibition of colicin synthesis by the antibiotic globomycin. Arch Microbiol. 1998;171:50-8 pubmed
    ..The Gbc phenotype was alleviated by a null mutation in the degP gene encoding the DegP/HtrA protease, abolished by a null mutation in the lpp gene encoding the murein-lipoprotein, ..
  66. Park H, Kim Y, Choi C, Kim S. Tripodal Lipoprotein Variants with C-Terminal Hydrophobic Residues Allosterically Modulate Activity of the DegP Protease. J Mol Biol. 2017;429:3090-3101 pubmed publisher
    b>DegP, a member of the highly conserved HtrA family of proteases, performs a regulated proteolysis of toxic misfolded proteins in the periplasm of Gram-negative bacteria...