Gene Symbol: cysM
Description: cysteine synthase B (O-acetylserine sulfhydrolase B)
Alias: ECK2416, JW2414
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Helling R, Janes B, Kimball H, Tran T, Bundesmann M, Check P, et al. Toxic waste disposal in Escherichia coli. J Bacteriol. 2002;184:3699-703 pubmed
    ..The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism. ..
  2. Sirko A, Hryniewicz M, Hulanicka D, Bock A. Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster. J Bacteriol. 1990;172:3351-7 pubmed
    ..Four open reading frames, designated cysT, cysW, cysA, and cysM, have been identified...
  3. Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, Nakamori S. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl Microbiol Biotechnol. 2003;62:239-43 pubmed
    ..These results indicated that TNase and CBL did act on L-cysteine degradation in E. coli cells. ..
  4. Claus M, Zocher G, Maier T, Schulz G. Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli. Biochemistry. 2005;44:8620-6 pubmed
    ..The structure of isoenzyme B (CysM) from Escherichia coli was established in a hexagonal crystal form at 2...
  5. Brun Y, Lapointe J. Locations of genes in the 52-minute region on the physical map of Escherichia coli K-12. J Bacteriol. 1990;172:4746-7 pubmed
  6. Akiyama Y, Shirai Y, Ito K. Involvement of FtsH in protein assembly into and through the membrane. II. Dominant mutations affecting FtsH functions. J Biol Chem. 1994;269:5225-9 pubmed
    ..It was suggested that the amino-terminal membrane region of FtsH interacts with other component(s), and that the two putative ATP binding sites are of vital importance for the FtsH functions. ..
  7. Saffen D, Presper K, Doering T, Roseman S. Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes. J Biol Chem. 1987;262:16241-53 pubmed
    Specialized lambda-transducing phages that carry the Escherichia coli genes ptsH, ptsI, crr, cysM, and cysA have been isolated, and the genes were subcloned in plasmid pBR322...
  8. Sirko A, Zatyka M, Hulanicka M. Identification of the Escherichia coli cysM gene encoding O-acetylserine sulphydrylase B by cloning with mini-Mu-lac containing a plasmid replicon. J Gen Microbiol. 1987;133:2719-25 pubmed
    ..Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.
  9. Awano N, Wada M, Mori H, Nakamori S, Takagi H. Identification and functional analysis of Escherichia coli cysteine desulfhydrases. Appl Environ Microbiol. 2005;71:4149-52 pubmed
    ..The gene disruption for each protein was significantly effective for overproduction of L-cysteine and L-cystine. Growth phenotype and transcriptional analyses suggest that tryptophanase contributes primarily to L-cysteine degradation. ..

More Information


  1. Hryniewicz M, Sirko A, PaƂucha A, Bock A, Hulanicka D. Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding. J Bacteriol. 1990;172:3358-66 pubmed
    ..Preliminary sequence data from the corresponding region of the Salmonella typhimurium chromosome showed strictly homologous counterparts of the E. coli cysP and cysT genes. ..
  2. Brun Y, Breton R, Lanouette P, Lapointe J. Precise mapping and comparison of two evolutionarily related regions of the Escherichia coli K-12 chromosome. Evolution of valU and lysT from an ancestral tRNA operon. J Mol Biol. 1990;214:825-43 pubmed
    ..4 kb-ptsH greater than -0.05 kb-pstI greater than -0.05 kb-crr greater than -cysM-cysA in the clockwise order (greater than and less than indicate the direction of transcription; kb, 10(3) bases)...
  3. Maier T. Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine-biosynthetic pathway. Nat Biotechnol. 2003;21:422-7 pubmed
    ..O-acetyl-L-serine extruded from the cells by way of the O-acetylserine efflux protein was amenable to further biotransformations. ..
  4. Zocher G, Wiesand U, Schulz G. High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. FEBS J. 2007;274:5382-9 pubmed
    The crystal structure of the dimeric O-acetylserine sulfhydrylase isozyme B from Escherichia coli (CysM), complexed with the substrate analog citrate, has been determined at 1.33 A resolution by X-ray diffraction analysis...
  5. Flint D, Tuminello J, Miller T. Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to par. J Biol Chem. 1996;271:16053-67 pubmed
  6. Zhao C, Kumada Y, Imanaka H, Imamura K, Nakanishi K. Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli. Protein Expr Purif. 2006;47:607-13 pubmed
    ..The cysM gene encoding OASS-B was cloned and the enzyme was overexpressed in E. coli using pUC19 with a lacUV5 promoter...
  7. Fuentes D, Fuentes E, Castro M, Perez J, Araya M, Chasteen T, et al. Cysteine metabolism-related genes and bacterial resistance to potassium tellurite. J Bacteriol. 2007;189:8953-60 pubmed
    ..Results showed that cell tolerance to tellurite correlates with the expression level of the cysteine metabolic genes and that these genes are up-regulated when tellurite is present in the growth medium...