Gene Symbol: cysH
Description: phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
Alias: ECK2757, JW2732
Species: Escherichia coli str. K-12 substr. MG1655
Products:     cysH

Top Publications

  1. Chartron J, Shiau C, Stout C, Carroll K. 3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle. Biochemistry. 2007;46:3942-51 pubmed
  2. Krone F, Westphal G, Meyer H, Schwenn J. PAPS-reductase of Escherichia coli. Correlating the N-terminal amino acid sequence with the DNA of gene cys H. FEBS Lett. 1990;260:6-9 pubmed
    ..The open reading frame extends 732 bases and encodes for a peptide of Mr = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme. ..
  3. Ostrowski J, Wu J, Rueger D, Miller B, Siegel L, Kredich N. Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrit. J Biol Chem. 1989;264:15726-37 pubmed
    ..The DNA sequences of cysI and cysH from S. typhimurium and E...
  4. Hunt C, Colless V, Smith M, Molasky D, Malo M, Loughlin R. Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12. J Gen Microbiol. 1987;133:2707-17 pubmed
    ..The direction of transcription of the cysD gene was established, and from physical evidence the size of the 'silent section' between the cysH and cysD genes was estimated to be at least 11 kb.
  5. Li C, Peck H, Przybyla A. Cloning of the 3'-phosphoadenylyl sulfate reductase and sulfite reductase genes from Escherichia coli K-12. Gene. 1987;53:227-34 pubmed
    The structural genes for 3'-phosphoadenylyl sulfate (PAPS) reductase (cysH) and sulfite reductase (alpha and beta subunits; EC and cysJ) of Escherichia coli K-12 have been cloned by complementation...
  6. Berendt U, Haverkamp T, Prior A, Schwenn J. Reaction mechanism of thioredoxin: 3'-phospho-adenylylsulfate reductase investigated by site-directed mutagenesis. Eur J Biochem. 1995;233:347-56 pubmed
    ..Alkylation by SH-group inhibitors irreversibly inactivates the enzyme. The structural gene (cysH) encodes for a small polypeptide with a single Cys residue located in a conserved cluster (KXECGI/LH) of amino ..
  7. Savage H, Montoya G, Svensson C, Schwenn J, Sinning I. Crystal structure of phosphoadenylyl sulphate (PAPS) reductase: a new family of adenine nucleotide alpha hydrolases. Structure. 1997;5:895-906 pubmed
    ..The open, reduced form of PAPS reductase is able to bind PAPS, whereas the closed oxidized form cannot. A movement between the two monomers of the dimer may allow this switch in conformation to occur. ..
  8. Lillig C, Prior A, Schwenn J, Aslund F, Ritz D, Vlamis Gardikas A, et al. New thioredoxins and glutaredoxins as electron donors of 3'-phosphoadenylylsulfate reductase. J Biol Chem. 1999;274:7695-8 pubmed
    ..9 microM), whereas the Km trx2 was considerably higher (34.2 microM). The results agree with previous in vivo data suggesting that Trx1 or Grx1 is essential for sulfate reduction but not for ribonucleotide reduction in E. coli. ..
  9. Helling R, Janes B, Kimball H, Tran T, Bundesmann M, Check P, et al. Toxic waste disposal in Escherichia coli. J Bacteriol. 2002;184:3699-703 pubmed
    ..Nal(r)) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation...

More Information


  1. Lillig C, Potamitou A, Schwenn J, Vlamis Gardikas A, Holmgren A. Redox regulation of 3'-phosphoadenylylsulfate reductase from Escherichia coli by glutathione and glutaredoxins. J Biol Chem. 2003;278:22325-30 pubmed
    ..However, it was absent in better growing cells expressing monothiol Grx2 C12S or wild type Grx2. Reversible glutathionylation may thus regulate the activity of PAPS reductase in vivo. ..
  2. Krone F, Westphal G, Schwenn J. Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli. Mol Gen Genet. 1991;225:314-9 pubmed
    The nucleotide sequence of the gene cysH from Escherichia coli K12 was determined...
  3. Tei H, Murata K, Kimura A. Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12. Biotechnol Appl Biochem. 1990;12:212-6 pubmed
    ..The following results were obtained. First, the cysCDHIJ regulon could be separated into cysCD and cysHIJ regions. Second, the cysG gene was quite different from the cysI and cysJ genes. ..
  4. De Cicco M, Macor F, Robieux I, Zanette G, Fantin D, Fabiani F, et al. Pharmacokinetic and pharmacodynamic effects of high-dose continuous intravenous verapamil infusion: clinical experience in the intensive care unit. Crit Care Med. 1999;27:332-9 pubmed
    ..In the absence of heart disease, following a step by step increase of the dosage, the high plasma verapamil concentrations (617 to 2970 ng/mL) produce frequent but well tolerated hemodynamic and electrocardiogram changes. ..
  5. Rossi E, Motta S, Mauri P, Landini P. Sulfate assimilation pathway intermediate phosphoadenosine 59-phosphosulfate acts as a signal molecule affecting production of curli fibres in Escherichia coli. Microbiology. 2014;160:1832-44 pubmed publisher
    ..In this work, we show that inactivation of the cysH gene, leading to accumulation of phosphoadenosine 59-phosphosulfate (PAPS), also an intermediate of the sulfate ..