Gene Symbol: cysG
Description: fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase
Alias: ECK3356, JW3331
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Spencer J, Stolowich N, Roessner C, Scott A. The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase. FEBS Lett. 1993;335:57-60 pubmed
    Previously, the E. coli cysG gene product had been shown to sequentially methylate uro'gen III to produce precorrin-2, hence it was given the trivial name uro'gen III methylase...
  2. Woodcock S, Raux E, Levillayer F, Thermes C, Rambach A, Warren M. Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of sirohaem synthase (CysG) on sirohaem and cobalamin biosynthesis. Biochem J. 1998;330 ( Pt 1):121-9 pubmed
    The Escherichia coli CysG protein (sirohaem synthase) catalyses four separate reactions that are required for the transformation of uroporphyrinogen III into sirohaem, initially two S-adenosyl-l-methionine-dependent transmethylations at ..
  3. Wu J, Siegel L, Kredich N. High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor. J Bacteriol. 1991;173:325-33 pubmed
    ..overexpression of holoenzyme activity was achieved by the inclusion in such plasmids of Salmonella typhimurium cysG, which encodes a uroporphyrinogen III methyltransferase required for the synthesis of siroheme, a cofactor for the ..
  4. Kulakauskas S, Sukhodolets V. [Generation of deletions in the region of the crp gene on the Escherichia coli chromosome]. Genetika. 1985;21:1068-9 pubmed
    Deletions in the argD, crp, cysG genes (73-74 min of the Escherichia coli genetic map) were obtained by heat induction of the phage lambda c1857 b221 rex::Tn5 integrated previously into the cysG gene by homologous recombination in the ..
  5. Warren M, Roessner C, Santander P, Scott A. The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase. Biochem J. 1990;265:725-9 pubmed
    The Escherichia coli cysG gene was successfully subcloned and over-expressed to produce a 52 kDa protein that was purified to homogeneity...
  6. Harborne N, Griffiths L, Busby S, Cole J. Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon. Mol Microbiol. 1992;6:2805-13 pubmed
    Five open reading frames designated nirB, nirD, nirE, nirC and cysG have been identified from the DNA sequence of the Escherichia coli nir operon...
  7. Warren M, Stolowich N, Santander P, Roessner C, Sowa B, Scott A. Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase. FEBS Lett. 1990;261:76-80 pubmed
    ..coli harbouring a plasmid containing the cysG gene...
  8. Macdonald H, Cole J. Molecular cloning and functional analysis of the cysG and nirB genes of Escherichia coli K12, two closely-linked genes required for NADH-dependent nitrite reductase activity. Mol Gen Genet. 1985;200:328-34 pubmed
    We have cloned two genes, nirB+ and cysG+ which are required for NADH-dependent nitrite reductase to be active, from the 74 min region of the Escherichia coli chromosome...
  9. Bell A, Gaston K, Cole J, Busby S. Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP. Nucleic Acids Res. 1989;17:3865-74 pubmed
    ..Single changes at either of two symmetrically-related positions create sites that are recognised by both FNR and CRP. Changes at both positions result in a site that is not recognised by FNR but which binds CRP tightly. ..

More Information


  1. Jackson R, Cornish Bowden A, Cole J. Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12. Biochem J. 1981;193:861-7 pubmed
    ..The presence of sirohaem in E. coli nitrite reductase explains the apparent identity of the cysG and nirB gene of E. coli and inability of hemA mutants to reduce nitrite.
  2. Warren M, Bolt E, Roessner C, Scott A, Spencer J, Woodcock S. Gene dissection demonstrates that the Escherichia coli cysG gene encodes a multifunctional protein. Biochem J. 1994;302 ( Pt 3):837-44 pubmed
    The C-terminus of the Escherichia coli CysG protein, consisting of amino acids 202-457, was expressed as a recombinant protein using gene dissection methodology...
  3. Tei H, Murata K, Kimura A. Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12. Biotechnol Appl Biochem. 1990;12:212-6 pubmed
    The cysCDHIJ gene region at 59 min and the cysG gene at 74 min on the chromosome of Escherichia coli K-12 were cloned. The ability of these cloned genes to complement mutants was analyzed and their restriction maps were determined...
  4. Woodcock S, Warren M. Evidence for a covalent intermediate in the S-adenosyl-L-methionine-dependent transmethylation reaction catalysed by sirohaem synthase. Biochem J. 1996;313 ( Pt 2):415-21 pubmed
    b>CysG, also known as uroporphyrinogen III methylase and sirohaem synthase (CysG; EC 2.1.1...
  5. Macdonald H, Pope N, Cole J. Isolation, characterization and complementation analysis of nirB mutants of Escherichia coli deficient only in NADH-dependent nitrite reductase activity. J Gen Microbiol. 1985;131:2771-82 pubmed
    ..The defects map in a gene, nirB, which is extremely close to cysG, the gene order being crp, nirB, cysG, aroB...
  6. Cole J, Newman B, White P. Biochemical and genetic characterization of nirB mutants of Escherichia coli K 12 pleiotropically defective in nitrite and sulphite reduction. J Gen Microbiol. 1980;120:475-83 pubmed
    ..coli chromosome. Mapping and reversion studies indicate the nirB is identical to the previously described cysG gene...
  7. Peakman T, Busby S, Cole J. Transcriptional control of the cysG gene of Escherichia coli K-12 during aerobic and anaerobic growth. Eur J Biochem. 1990;191:325-31 pubmed
    ..The first and last of these genes, nirB and cysG, are transcribed in the same direction and both are essential for NADH-dependent nitrite reductase activity...
  8. Peakman T, Crouzet J, Mayaux J, Busby S, Mohan S, Harborne N, et al. Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome. Eur J Biochem. 1990;191:315-23 pubmed
    ..been determined in order to locate the structural gene, nirB, for the NADH-dependent nitrite reductase and a gene, cysG, required for the synthesis of the sirohaem prosthetic group...