Gene Symbol: cysC
Description: adenosine 5'-phosphosulfate kinase
Alias: ECK2745, JW2720
Species: Escherichia coli str. K-12 substr. MG1655
Tei H, Murata K, Kimura A. Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12. Biotechnol Appl Biochem. 1990;12:212-6 pubmed
..The following results were obtained. First, the cysCDHIJ regulon could be separated into cysCD and cysHIJ regions. Second, the cysG gene was quite different from the cysI and cysJ genes. ..
Satishchandran C, Markham G. Mechanistic studies of Escherichia coli adenosine-5'-phosphosulfate kinase. Arch Biochem Biophys. 2000;378:210-5 pubmed
..The affinity for Mn(2+) increases 23-fold when the enzyme is phosphorylated. Two Mn(2+) ions bind per subunit when both APS and the ATP analog AMPPNP are present, indicating a potential dual metal ion catalytic mechanism. ..
Leyh T, Taylor J, Markham G. The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization. J Biol Chem. 1988;263:2409-16 pubmed
..gene into plasmid or chromosomal cysN prevents sulfate activation and decreases expression of the downstream cysC gene. cysC appears to be the APS kinase structural gene and encodes a 21-kDa polypeptide...
Leyh T, Vogt T, Suo Y. The DNA sequence of the sulfate activation locus from Escherichia coli K-12. J Biol Chem. 1992;267:10405-10 pubmed
..The sequence includes the structural genes encoding the enzymes ATP sulfurylase (cysD and cysN) and APS kinase (cysC) which catalyze the synthesis of activated sulfate...
Satishchandran C, Hickman Y, Markham G. Characterization of the phosphorylated enzyme intermediate formed in the adenosine 5'-phosphosulfate kinase reaction. Biochemistry. 1992;31:11684-8 pubmed
..and thus to define the active site region of APS kinase, we have determined the complete sequence of cysC, the structural gene for this enzyme in E. coli...
Malo M, Loughlin R. Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12. Gene. 1990;87:127-31 pubmed
..Evidence is presented for a possible CysB protein binding site around -89, thought to be involved in regulation of expression of the cysD gene. ..
Satishchandran C, Markham G. Adenosine-5'-phosphosulfate kinase from Escherichia coli K12. Purification, characterization, and identification of a phosphorylated enzyme intermediate. J Biol Chem. 1989;264:15012-21 pubmed
..The substrate inhibition by APS is consistent with APS binding to the enzyme, to form a dead-end E.APS complex. ..
Hunt C, Colless V, Smith M, Molasky D, Malo M, Loughlin R. Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12. J Gen Microbiol. 1987;133:2707-17 pubmed
..The direction of transcription of the cysD gene was established, and from physical evidence the size of the 'silent section' between the cysH and cysD genes was estimated to be at least 11 kb. ..
Fuentes D, Fuentes E, Castro M, Perez J, Araya M, Chasteen T, et al
. Cysteine metabolism-related genes and bacterial resistance to potassium tellurite. J Bacteriol. 2007;189:8953-60 pubmed
..Results showed that cell tolerance to tellurite correlates with the expression level of the cysteine metabolic genes and that these genes are up-regulated when tellurite is present in the growth medium...