cysB

Summary

Gene Symbol: cysB
Description: N-acetylserine-responsive cysteine regulon transcriptional activator; autorepressor
Alias: ECK1269, JW1267
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Tyrrell R, Verschueren K, Dodson E, Murshudov G, Addy C, Wilkinson A. The structure of the cofactor-binding fragment of the LysR family member, CysB: a familiar fold with a surprising subunit arrangement. Structure. 1997;5:1017-32 pubmed
    b>CysB is a tetrameric protein of identical subunits (M(r) = 36,000) which controls the expression of genes associated with the biosynthesis of cysteine in bacteria...
  2. Gyaneshwar P, Paliy O, McAuliffe J, Popham D, Jordan M, Kustu S. Sulfur and nitrogen limitation in Escherichia coli K-12: specific homeostatic responses. J Bacteriol. 2005;187:1074-90 pubmed
    ..tools, we confirmed known homeostatic responses to global S limitation, which are controlled by the activators CysB and Cbl, and found that S limitation propagated into methionine metabolism, synthesis of FeS clusters, and ..
  3. Jovanovic M, Lilic M, Savic D, Jovanovic G. The LysR-type transcriptional regulator CysB controls the repression of hslJ transcription in Escherichia coli. Microbiology. 2003;149:3449-59 pubmed
    The LysR-type transcriptional regulator (LTTR) CysB is a transcription factor in Escherichia coli cells, where as a homotetramer it binds the target promoter regions and activates the genes involved in sulphur utilization and sulphonate-..
  4. Ostrowski J, Jagura Burdzy G, Kredich N. DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli. J Biol Chem. 1987;262:5999-6005 pubmed
    Nucleotide sequences of the cysB region of Salmonella typhimurium and Escherichia coli have been determined and compared. A total of 1759 nucleotides were sequenced in S. typhimurium and 1840 in E. coli...
  5. Bielinska A, Hulanicka D. Regulation of the cysB gene expression in Escherichia coli. Acta Biochim Pol. 1986;33:133-7 pubmed
    It was found by Northern-type hybridization that the amount of RNA, the transcription of which begins at the cysB regulatory gene promoter, is significantly reduced after cysB gene introduction into Escherichia coli on multicopy plasmid...
  6. van der Ploeg J, Eichhorn E, Leisinger T. Sulfonate-sulfur metabolism and its regulation in Escherichia coli. Arch Microbiol. 2001;176:1-8 pubmed
    ..Expression of the tau and ssu genes requires the LysR-type transcriptional regulatory proteins CysB and Cbl...
  7. Lochowska A, Iwanicka Nowicka R, Plochocka D, Hryniewicz M. Functional dissection of the LysR-type CysB transcriptional regulator. Regions important for DNA binding, inducer response, oligomerization, and positive control. J Biol Chem. 2001;276:2098-107 pubmed
    b>CysB is a tetrameric LysR-type transcriptional regulator that acts as an activator of cys regulon genes and as an autorepressor. Positive control of cys genes requires the presence of the inducer N-acetylserine...
  8. van der Ploeg J, Iwanicka Nowicka R, Bykowski T, Hryniewicz M, Leisinger T. The Escherichia coli ssuEADCB gene cluster is required for the utilization of sulfur from aliphatic sulfonates and is regulated by the transcriptional activator Cbl. J Biol Chem. 1999;274:29358-65 pubmed
    ..b>CysB, the LysR transcriptional regulator for the cys genes, was not required for expression of a chromosomal ssuE'-lacZ ..
  9. Karpinski P, Grudniak A, Wolska K. Effect of mutations in dnaK and dnaJ genes on cysteine operon expression in Escherichia coli. Folia Microbiol (Praha). 2002;47:371-4 pubmed
    ..The presence of the pMH224 plasmid coding for CysB* protein defective in DNA binding lowered beta-galactosidase expression from cysPTWA::lacZ fusion strain harboring ..

More Information

Publications27

  1. Rowbury R. Regulatory components, including integration host factor, CysB and H-NS, that influence pH responses in Escherichia coli. Lett Appl Microbiol. 1997;24:319-28 pubmed
    ..Bending may allow transcription by bringing activators into juxtaposition with RNA polymerase, which is CysB-associated in several of the responses...
  2. Lilic M, Jovanovic M, Jovanovic G, Savic D. Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype. FEMS Microbiol Lett. 2003;224:239-46 pubmed
    The cysB gene product is a LysR-type regulatory protein required for expression of the cys regulon...
  3. Jagura Burdzy G, Hulanicka D. Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon. J Bacteriol. 1981;147:744-51 pubmed
    Strains of escherichia coli were constructed in which the lacZ gene is fused to cysB, the positive regulator gene of the cysteine regulon...
  4. Bielinska A, Hulanicka D. Effect of DNA gyrase inhibitors and urea on the expression of cysB, the regulatory gene of the cysteine regulon. J Gen Microbiol. 1986;132:2571-6 pubmed
    b>cysB, the regulatory gene of the cysteine regulon, is autoregulated. Inhibitors of both gyrase subunits, nalidixic acid and novobiocin, affect the expression of cysB, as monitored by beta-galactosidase activity in cysB::lac fusion strains...
  5. Awano N, Wada M, Mori H, Nakamori S, Takagi H. Identification and functional analysis of Escherichia coli cysteine desulfhydrases. Appl Environ Microbiol. 2005;71:4149-52 pubmed
    ..The gene disruption for each protein was significantly effective for overproduction of L-cysteine and L-cystine. Growth phenotype and transcriptional analyses suggest that tryptophanase contributes primarily to L-cysteine degradation. ..
  6. Lamark T, Kaasen I, Eshoo M, Falkenberg P, McDougall J, Strøm A. DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol Microbiol. 1991;5:1049-64 pubmed
    ..The bet genes are tightly spaced, with betT located upstream of, and transcribed divergently to, the tandemly linked betIBA genes. ..
  7. Rakonjac J, Milic M, Savic D. cysB and cysE mutants of Escherichia coli K12 show increased resistance to novobiocin. Mol Gen Genet. 1991;228:307-11 pubmed
    Mutations in the cysB and cysE genes of Escherichia coli K12 cause an increase in resistance to the gyrase inhibitor novobiocin but not to coumermycin, acriflavine and rifampicin...
  8. Bykowski T, van der Ploeg J, Iwanicka Nowicka R, Hryniewicz M. The switch from inorganic to organic sulphur assimilation in Escherichia coli: adenosine 5'-phosphosulphate (APS) as a signalling molecule for sulphate excess. Mol Microbiol. 2002;43:1347-58 pubmed
    ..from the ssu promoter, Cbl acts as a direct and sufficient activator of transcription in vivo and in vitro, whereas CysB downregulates the promoter efficiency...
  9. Kredich N. The molecular basis for positive regulation of cys promoters in Salmonella typhimurium and Escherichia coli. Mol Microbiol. 1992;6:2747-53 pubmed
    ..genes required for cysteine biosynthesis in Salmonella typhimurium and Escherichia coli are positively regulated by cysB, which encodes a transcriptional activator belonging to the LysR family of regulatory proteins...
  10. Isak G, Ryden Aulin M. Hypomodification of the wobble base in tRNAGlu, tRNALys, and tRNAGln suppresses the temperature-sensitive phenotype caused by mutant release factor 1. J Bacteriol. 2009;191:1604-9 pubmed publisher
    ..This study describes one such suppressor that is the result of an IS10 insertion into the cysB gene, giving a Cys(-) phenotype...
  11. Leyh T, Taylor J, Markham G. The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization. J Biol Chem. 1988;263:2409-16 pubmed
    ..The genes are adjacent and are transcribed counterclockwise on the E. coli chromosome in the order cysDNC. cysN and cysC are within the same operon and cysDNC are not in an operon containing cysHIJ. ..
  12. Tei H, Watanabe K, Murata K, Kimura A. Analysis of the Escherichia coli K-12 cysB gene and its product using the method of gene fusion. Biochem Biophys Res Commun. 1990;167:962-9 pubmed
    The amino-terminal structure and the essential functional region of the cysB gene product of Escherichia coli K-12 were analyzed by the method of gene fusion...
  13. Prodromou C, Artymiuk P, Guest J. The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases. Eur J Biochem. 1992;204:599-609 pubmed
    ..The acn gene is flanked by the cysB gene and a putative riboflavin biosynthesis gene resembling the ribA gene of Bacillus subtilis...
  14. Castanie Cornet M, Penfound T, Smith D, Elliott J, Foster J. Control of acid resistance in Escherichia coli. J Bacteriol. 1999;181:3525-35 pubmed
    ..5 acid challenge, the structural gene for arginine decarboxylase (adiA), and the regulator cysB, confirming earlier reports. The third AR system required glutamate for protection at pH 2...
  15. van der Ploeg J, Iwanicka Nowicka R, Kertesz M, Leisinger T, Hryniewicz M. Involvement of CysB and Cbl regulatory proteins in expression of the tauABCD operon and other sulfate starvation-inducible genes in Escherichia coli. J Bacteriol. 1997;179:7671-8 pubmed
    ..The protein encoded by cbl shows high sequence similarity to CysB, the LysR-type transcriptional activator of the genes involved in cysteine biosynthesis...
  16. Hryniewicz M, Kredich N. Stoichiometry of binding of CysB to the cysJIH, cysK, and cysP promoter regions of Salmonella typhimurium. J Bacteriol. 1994;176:3673-82 pubmed
    b>CysB is a member of the LysR family of transcriptional activators and regulates genes of the cysteine regulon in Salmonella typhimurium and Escherichia coli...
  17. Lochowska A, Iwanicka Nowicka R, Zaim J, Witkowska Zimny M, Bolewska K, Hryniewicz M. Identification of activating region (AR) of Escherichia coli LysR-type transcription factor CysB and CysB contact site on RNA polymerase alpha subunit at the cysP promoter. Mol Microbiol. 2004;53:791-806 pubmed
    b>CysB is a LysR-type transcriptional regulator (LTTR) controlling the expression of numerous genes involved in bacterial sulphur assimilation via cysteine biosynthesis...
  18. Monroe R, Ostrowski J, Hryniewicz M, Kredich N. In vitro interactions of CysB protein with the cysK and cysJIH promoter regions of Salmonella typhimurium. J Bacteriol. 1990;172:6919-29 pubmed
    The cysteine regulons of Salmonella typhimurium and Escherichia coli are positively regulated by CysB protein and either O-acetyl-L-serine or N-acetyl-L-serine, both of which act as inducers...