Gene Symbol: cynT
Description: carbonic anhydrase
Alias: ECK0336, JW0330
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Guilloton M, Korte J, Lamblin A, Fuchs J, Anderson P. Carbonic anhydrase in Escherichia coli. A product of the cyn operon. J Biol Chem. 1992;267:3731-4 pubmed
    The product of the cynT gene of the cyn operon in Escherichia coli has been identified as a carbonic anhydrase. The cyn operon also includes the gene cynS, encoding the enzyme cyanase...
  2. Hashimoto M, Kato J. Indispensability of the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure. Biosci Biotechnol Biochem. 2003;67:919-22 pubmed
    We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli...
  3. Kozliak E, Fuchs J, Guilloton M, Anderson P. Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate. J Bacteriol. 1995;177:3213-9 pubmed
    ..The gene for cyanase is part of the cyn operon, which includes cynT and cynS, encoding carbonic anhydrase and cyanase, respectively...
  4. Guilloton M, Lamblin A, Kozliak E, Gerami Nejad M, Tu C, Silverman D, et al. A physiological role for cyanate-induced carbonic anhydrase in Escherichia coli. J Bacteriol. 1993;175:1443-51 pubmed
    ..A carbonic anhydrase activity was recently found to be encoded by the cynT gene, the first gene of the cyn operon; it was proposed that carbonic anhydrase prevents depletion of bicarbonate ..
  5. Anderson P, Sung Y, Fuchs J. The cyanase operon and cyanate metabolism. FEMS Microbiol Rev. 1990;7:247-52 pubmed
    ..It is encoded as part of an operon we have named the cyn operon, which includes three genes in the following order: cynT (cyanate permease), cynS (cyanase), and cynX (protein of unknown function)...
  6. Sung Y, Parsell D, Anderson P, Fuchs J. Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12. J Bacteriol. 1987;169:2639-42 pubmed
    ..Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source. ..
  7. Sung Y, Fuchs J. Characterization of the cyn operon in Escherichia coli K12. J Biol Chem. 1988;263:14769-75 pubmed
    ..The cyn operon is approximately 2600 base pairs and includes cynT, cynS, and cynX, which encode cyanate permease, cyanase, and a protein of unknown function, respectively...
  8. Smith K, Ferry J. Prokaryotic carbonic anhydrases. FEMS Microbiol Rev. 2000;24:335-66 pubmed
    ..Investigations into prokaryotic carbonic anhydrase have already led to the identification of a new class (gamma) and future research will undoubtedly reveal novel functions for carbonic anhydrase in prokaryotes. ..
  9. Sung Y, Fuchs J. Identification and characterization of a cyanate permease in Escherichia coli K-12. J Bacteriol. 1989;171:4674-8 pubmed
    ..The 26-kDa protein was identified as a cyanate permease that transports exogenous cyanate by active transport. E. coli was shown to contain a cyanate transport system that is energy dependent and saturable by cyanate. ..

More Information


  1. Lamblin A, Fuchs J. Functional analysis of the Escherichia coli K-12 cyn operon transcriptional regulation. J Bacteriol. 1994;176:6613-22 pubmed
    ..CynR binding was not affected by cyanate or its structural homolog azide, a gratuitous inducer of the operon. However, CynR-induced bending of two different DNA fragments was detected. The amount of bending was decreased by cyanate. ..
  2. Kozliak E, Guilloton M, Gerami Nejad M, Fuchs J, Anderson P. Expression of proteins encoded by the Escherichia coli cyn operon: carbon dioxide-enhanced degradation of carbonic anhydrase. J Bacteriol. 1994;176:5711-7 pubmed
    ..The cynS gene encoding cyanase, together with the cynT gene for carbonic anhydrase, is part of the cyn operon, the expression of which is induced in Escherichia coli by ..
  3. Kozliak E, Guilloton M, Fuchs J, Anderson P. Bacterial carbonic anhydrases. EXS. 2000;:547-65 pubmed
  4. Tripp B, Smith K, Ferry J. Carbonic anhydrase: new insights for an ancient enzyme. J Biol Chem. 2001;276:48615-8 pubmed
  5. Sung Y, Anderson P, Fuchs J. Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase. J Bacteriol. 1987;169:5224-30 pubmed
    ..The cynS gene was located next to the lac operon, and the direction of cynS transcription was opposite that of lac. ..
  6. Merlin C, Masters M, McAteer S, Coulson A. Why is carbonic anhydrase essential to Escherichia coli?. J Bacteriol. 2003;185:6415-24 pubmed
    ..b>CynT, the single E...