Gene Symbol: cutA
Description: divalent-cation tolerance protein, copper sensitivity
Alias: ECK4131, JW4097, cutA1, cycY
Species: Escherichia coli str. K-12 substr. MG1655
Arnesano F, Banci L, Benvenuti M, Bertini I, Calderone V, Mangani S, et al
. The evolutionarily conserved trimeric structure of CutA1 proteins suggests a role in signal transduction. J Biol Chem. 2003;278:45999-6006 pubmed
b>CutA1 are a protein family present in bacteria, plants, and animals, including humans...
Tanaka T, Sawano M, Ogasahara K, Sakaguchi Y, Bagautdinov B, Katoh E, et al
. Hyper-thermostability of CutA1 protein, with a denaturation temperature of nearly 150 degrees C. FEBS Lett. 2006;580:4224-30 pubmed
We found that the CutA1 protein, from Pyrococcus horikoshii (PhCutA1), has an extremely high denaturation temperature (T(d)) of nearly 150 degrees C, which exceeds the highest record determined by DSC by about 30 degrees C...
Fong S, Camakaris J, Lee B. Molecular genetics of a chromosomal locus involved in copper tolerance in Escherichia coli K-12. Mol Microbiol. 1995;15:1127-37 pubmed
The cutA locus, presumably involved in copper tolerance in Escherichia coli, was characterized by a mutation leading to copper sensitivity...
Crooke H, Cole J. The biogenesis of c-type cytochromes in Escherichia coli requires a membrane-bound protein, DipZ, with a protein disulphide isomerase-like domain. Mol Microbiol. 1995;15:1139-50 pubmed
..It is suggested that DipZ is essential for maintaining cytochrome c apoproteins in the correct conformations for the covalent attachment of haem groups to the appropriate pairs of cysteine residues. ..
Iobbi Nivol C, Crooke H, Griffiths L, Grove J, Hussain H, Pommier J, et al
. A reassessment of the range of c-type cytochromes synthesized by Escherichia coli K-12. FEMS Microbiol Lett. 1994;119:89-94 pubmed
..No other c-type cytochrome was detected under any growth condition tested. ..
Sambongi Y, Crooke H, Cole J, Ferguson S. A mutation blocking the formation of membrane or periplasmic endogenous and exogenous c-type cytochromes in Escherichia coli permits the cytoplasmic formation of Hydrogenobacter thermophilus holo cytochrome c552. FEBS Lett. 1994;344:207-10 pubmed
..thermophilus cytochrome c552 in E. coli does not involve the physiological pathway of c-type cytochrome biosynthesis in E. coli and that the haem insertion may be catalysed...
Bertini I, Jimenez B, Pierattelli R, Wedd A, Xiao Z. Protonless 13C direct detection NMR: characterization of the 37 kDa trimeric protein CutA1. Proteins. 2008;70:1196-205 pubmed
..We applied this novel methodology to the study of the CutA1 protein (12.3 kDa) from E. coli that forms a homotrimer in solution with a total molecular mass of 37 kDa...
Matsuura Y, Ota M, Tanaka T, Takehira M, Ogasahara K, Bagautdinov B, et al
. Remarkable improvement in the heat stability of CutA1 from Escherichia coli by rational protein design. J Biochem. 2010;148:449-58 pubmed publisher
To enhance the heat stability of the CutA1 protein from Escherichia coli (EcCutA1) so that it has comparable stability to CutA1 from Pyrococcus horikoshii with a denaturation temperature (T(d)) of 150°C, we used the Stability Profile of ..